The Experts below are selected from a list of 32586 Experts worldwide ranked by ideXlab platform
Leonor Silveira - One of the best experts on this subject based on the ideXlab platform.
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characterization of multidrug resistant isolates of salmonella enterica serovars heidelberg and minnesota from fresh Poultry Meat imported to portugal
Microbial Drug Resistance, 2021Co-Authors: Leonor Silveira, Alexandra Nunes, ângela Pista, Joana Isidro, Cristina Correia, Margarida Saraiva, Rita Batista, Isabel Castanheira, Jorge Machado, Joao Paulo GomesAbstract:Salmonella enterica serovars Heidelberg and Minnesota frequently display several genetic mobile elements making them potential spreaders of resistance genes. Here, we phenotypically determined the antibiotic resistance profile and subsequently performed whole-genome sequencing on 36 isolates recovered from samples of fresh Poultry Meat, within the Portuguese Official Inspection Plan for Imported Foodstuffs. Several isolates of both serovars showed high genetic relatedness either with isolates from raw Poultry Meat imported to the Netherlands from Brazil or with isolates from samples from the broiler production chain in Brazil. The multidrug-resistant (MDR) character was common to the vast majority (94.4%) of isolates from both serovars, and several isolates carried the plasmid IncA/C2 containing the β-lactamase gene blaCMY-2 and IncX1 containing a type IV secretion system. These results somehow mirror the scenario observed in the Netherlands, showing the introduction, through fresh imported Poultry Meat in compliance with European legislation, of MDR Salmonella enterica serovars Heidelberg and Minnesota in Europe, with the potential spread of resistance markers. These data suggest the need to revise the hygiene criteria for foodstuffs monitoring before its placement on the market, with the determination of the resistome being an invaluable contribute to limit the dissemination of resistance markers.
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imported Poultry Meat as a source of extended spectrum cephalosporin resistant cmy 2 producing salmonella heidelberg and salmonella minnesota in the european union 2014 2015
International Journal of Antimicrobial Agents, 2018Co-Authors: Joana Campos, Joana Mourão, Luísa Peixe, Leonor Silveira, Cristina Correia, Margarida Saraiva, Ana Paula Macas, Patrícia AntunesAbstract:Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of Poultry Meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from Poultry Meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh Meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported Poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant Poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations.
Margarida Saraiva - One of the best experts on this subject based on the ideXlab platform.
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characterization of multidrug resistant isolates of salmonella enterica serovars heidelberg and minnesota from fresh Poultry Meat imported to portugal
Microbial Drug Resistance, 2021Co-Authors: Leonor Silveira, Alexandra Nunes, ângela Pista, Joana Isidro, Cristina Correia, Margarida Saraiva, Rita Batista, Isabel Castanheira, Jorge Machado, Joao Paulo GomesAbstract:Salmonella enterica serovars Heidelberg and Minnesota frequently display several genetic mobile elements making them potential spreaders of resistance genes. Here, we phenotypically determined the antibiotic resistance profile and subsequently performed whole-genome sequencing on 36 isolates recovered from samples of fresh Poultry Meat, within the Portuguese Official Inspection Plan for Imported Foodstuffs. Several isolates of both serovars showed high genetic relatedness either with isolates from raw Poultry Meat imported to the Netherlands from Brazil or with isolates from samples from the broiler production chain in Brazil. The multidrug-resistant (MDR) character was common to the vast majority (94.4%) of isolates from both serovars, and several isolates carried the plasmid IncA/C2 containing the β-lactamase gene blaCMY-2 and IncX1 containing a type IV secretion system. These results somehow mirror the scenario observed in the Netherlands, showing the introduction, through fresh imported Poultry Meat in compliance with European legislation, of MDR Salmonella enterica serovars Heidelberg and Minnesota in Europe, with the potential spread of resistance markers. These data suggest the need to revise the hygiene criteria for foodstuffs monitoring before its placement on the market, with the determination of the resistome being an invaluable contribute to limit the dissemination of resistance markers.
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imported Poultry Meat as a source of extended spectrum cephalosporin resistant cmy 2 producing salmonella heidelberg and salmonella minnesota in the european union 2014 2015
International Journal of Antimicrobial Agents, 2018Co-Authors: Joana Campos, Joana Mourão, Luísa Peixe, Leonor Silveira, Cristina Correia, Margarida Saraiva, Ana Paula Macas, Patrícia AntunesAbstract:Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of Poultry Meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from Poultry Meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh Meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported Poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant Poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations.
Cristina Correia - One of the best experts on this subject based on the ideXlab platform.
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characterization of multidrug resistant isolates of salmonella enterica serovars heidelberg and minnesota from fresh Poultry Meat imported to portugal
Microbial Drug Resistance, 2021Co-Authors: Leonor Silveira, Alexandra Nunes, ângela Pista, Joana Isidro, Cristina Correia, Margarida Saraiva, Rita Batista, Isabel Castanheira, Jorge Machado, Joao Paulo GomesAbstract:Salmonella enterica serovars Heidelberg and Minnesota frequently display several genetic mobile elements making them potential spreaders of resistance genes. Here, we phenotypically determined the antibiotic resistance profile and subsequently performed whole-genome sequencing on 36 isolates recovered from samples of fresh Poultry Meat, within the Portuguese Official Inspection Plan for Imported Foodstuffs. Several isolates of both serovars showed high genetic relatedness either with isolates from raw Poultry Meat imported to the Netherlands from Brazil or with isolates from samples from the broiler production chain in Brazil. The multidrug-resistant (MDR) character was common to the vast majority (94.4%) of isolates from both serovars, and several isolates carried the plasmid IncA/C2 containing the β-lactamase gene blaCMY-2 and IncX1 containing a type IV secretion system. These results somehow mirror the scenario observed in the Netherlands, showing the introduction, through fresh imported Poultry Meat in compliance with European legislation, of MDR Salmonella enterica serovars Heidelberg and Minnesota in Europe, with the potential spread of resistance markers. These data suggest the need to revise the hygiene criteria for foodstuffs monitoring before its placement on the market, with the determination of the resistome being an invaluable contribute to limit the dissemination of resistance markers.
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imported Poultry Meat as a source of extended spectrum cephalosporin resistant cmy 2 producing salmonella heidelberg and salmonella minnesota in the european union 2014 2015
International Journal of Antimicrobial Agents, 2018Co-Authors: Joana Campos, Joana Mourão, Luísa Peixe, Leonor Silveira, Cristina Correia, Margarida Saraiva, Ana Paula Macas, Patrícia AntunesAbstract:Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of Poultry Meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from Poultry Meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh Meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported Poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant Poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations.
Patrícia Antunes - One of the best experts on this subject based on the ideXlab platform.
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imported Poultry Meat as a source of extended spectrum cephalosporin resistant cmy 2 producing salmonella heidelberg and salmonella minnesota in the european union 2014 2015
International Journal of Antimicrobial Agents, 2018Co-Authors: Joana Campos, Joana Mourão, Luísa Peixe, Leonor Silveira, Cristina Correia, Margarida Saraiva, Ana Paula Macas, Patrícia AntunesAbstract:Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of Poultry Meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from Poultry Meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh Meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported Poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant Poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations.
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Salmonellosis: the role of Poultry Meat.
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2015Co-Authors: Patrícia Antunes, Joana Mourão, Joana Campos, Luísa PeixeAbstract:Salmonellosis remains one of the most frequent food-borne zoonoses, constituting a worldwide major public health concern. Currently, at a global level, the main sources of infection for humans include Meat products, including the consumption of contaminated Poultry Meat, in spite of the success of Salmonella control measures implemented in food-animal production of industrialized countries. In recent years, a shift in Salmonella serotypes related to Poultry and Poultry production has been reported in diverse geographical regions, being particularly associated with the spread of certain well-adapted clones. Moreover, antimicrobial resistance in non-typhoidal Salmonella is considered one of the major public health threats related with food-animal production, including the Poultry production chain and Poultry Meat, which is an additional concern in the management of salmonellosis. The circulation of the same multidrug-resistant Salmonella clones and/or identical mobile genetic elements encoding antibiotic resistance genes from Poultry to humans highlights this scenario. The purpose of this review was to provide an overview of the role of Poultry Meat on salmonellosis at a global scale and the main problems that could hinder the success of Salmonella control measures at animal production level. With the increasing globalization of foodstuffs like Poultry Meat, new problems and challenges might arise regarding salmonellosis control, making new integrated intervention strategies necessary along the food chain.
Imca Sampers - One of the best experts on this subject based on the ideXlab platform.
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survey of belgian consumption patterns and consumer behaviour of Poultry Meat to provide insight in risk factors for campylobacteriosis
Food Control, 2012Co-Authors: Imca Sampers, Dirk Berkvens, Liesbeth Jacxsens, Mariacristina Ciocci, Ann Dumoulin, Mieke UyttendaeleAbstract:Abstract A survey was set-up using a web-application supplemented with a print-out questionnaire to gather knowledge on consumption patterns and consumer behaviour in the storage and preparation of Poultry Meat, in particular Poultry Meat preparations for Belgian adults. In total 1618 respondents were reached. The survey included questions on risk factors associated with campylobacteriosis i.e. storage conditions, potential for cross-contamination and undercooking. Almost 61% of the respondents have identified home freezing of fresh Poultry Meat as a common storage technique. Several cross-contamination routes in the kitchen are confirmed, e.g. putting cooked Poultry Meat at the same plate of the raw Poultry Meat (11.2%), not washing hands after handling raw Poultry Meat (11%), using the same knife (6.7%) or cutting board (8.2%) for the vegetables which was prior used for the Poultry Meat. Although the majority of the respondents indicated to eat their Poultry Meat well-cooked (96% for Poultry Meat preparations to 98.4% for broiler), 16% would eat not well-cooked Poultry Meat if served at a barbecue. Poultry parts not marinated or seasoned was ranked as eaten the most, followed by broiler and next Meat preparations (with first the marinated/seasoned Poultry cuts and second the minced Poultry Meat preparations). The gathered consumer data may now be incorporated into a retail to fork quantitative risk assessment model for Poultry Meat preparations to estimate the risk for campylobacteriosis to the Belgian consumer and to enable the evaluation of risk mitigation strategies (to support risk managers) in the consumer phase. A proper comparison of the contribution of the different pathways to consumers’ exposure (i.e. effect of freezing during storage, prevalence of undercooking and cross-contamination) to Campylobacter bacteria may now be performed.
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performance of food safety management systems in Poultry Meat preparation processing plants in relation to campylobacter spp contamination
Journal of Food Protection, 2010Co-Authors: Imca Sampers, Liesbeth Jacxsens, Ann Dumoulin, Pieternel A Luning, Willem J Marcelis, Mieke UyttendaeleAbstract:A diagnostic instrument comprising a combined assessment of core control and assurance activities and a microbial assessment instrument were used to measure the performance of current food safety management systems (FSMSs) of two Poultry Meat preparation companies. The high risk status of the company's contextual factors, i.e., starting from raw materials (Poultry carcasses) with possible high numbers and prevalence of pathogens such as Campylobacter spp., requires advanced core control and assurance activities in the FSMS to guarantee food safety. The level of the core FSMS activities differed between the companies, and this difference was reflected in overall microbial quality (mesophilic aerobic count), presence of hygiene indicators (Enterobacteriaceae, Staphylococcus aureus, and Escherichia coli), and contamination with pathogens such as Salmonella, Listeria monocytogenes, and Campylobacter spp. The food safety output expressed as a microbial safety profile was related to the variability in the prevalence and contamination levels of Campylobacter spp. in Poultry Meat preparations found in a Belgian nationwide study. Although a Poultry Meat processing company could have an advanced FSMS in place and a good microbial profile (i.e., lower prevalence of pathogens, lower microbial numbers, and less variability in microbial contamination), these positive factors might not guarantee pathogen-free products. Contamination could be attributed to the inability to apply effective interventions to reduce or eliminate pathogens in the production chain of (raw) Poultry Meat preparations.