The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Krzysztof Lewiński - One of the best experts on this subject based on the ideXlab platform.
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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.
International journal of biological macromolecules, 2015Co-Authors: J.i. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Krzysztof LewińskiAbstract:Abstract Interactions between bovine and goat β-lactoglobulin and tetracaine and Pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and Pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that Pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 A revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and Pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein–drug interactions.
J.i. Loch - One of the best experts on this subject based on the ideXlab platform.
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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.
International journal of biological macromolecules, 2015Co-Authors: J.i. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Krzysztof LewińskiAbstract:Abstract Interactions between bovine and goat β-lactoglobulin and tetracaine and Pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and Pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that Pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 A revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and Pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein–drug interactions.
Piotr Bonarek - One of the best experts on this subject based on the ideXlab platform.
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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.
International journal of biological macromolecules, 2015Co-Authors: J.i. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Krzysztof LewińskiAbstract:Abstract Interactions between bovine and goat β-lactoglobulin and tetracaine and Pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and Pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that Pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 A revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and Pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein–drug interactions.
Agnieszka Polit - One of the best experts on this subject based on the ideXlab platform.
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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.
International journal of biological macromolecules, 2015Co-Authors: J.i. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Krzysztof LewińskiAbstract:Abstract Interactions between bovine and goat β-lactoglobulin and tetracaine and Pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and Pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that Pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 A revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and Pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein–drug interactions.
Mateusz Jabłoński - One of the best experts on this subject based on the ideXlab platform.
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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.
International journal of biological macromolecules, 2015Co-Authors: J.i. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Krzysztof LewińskiAbstract:Abstract Interactions between bovine and goat β-lactoglobulin and tetracaine and Pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and Pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that Pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 A revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and Pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein–drug interactions.