The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
James Mccluskey - One of the best experts on this subject based on the ideXlab platform.
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restricted epitope recognition by Precipitin negative anti la ss b positive sera
Arthritis & Rheumatism, 1992Co-Authors: Tom P Gordon, Chris Mavrangelos, James MccluskeyAbstract:Objective. To determine whether anti–La/SS-B–positive sera that are Precipitin negative show a distinct B cell epitope pattern. Methods. Serum reactivity was tested with recombinant La/SS-B fusion proteins. Results. Among the 18 Precipitin-negative anti-La/SS-B–positive sera, reactivity was confined to the full-length recombinant protein (La33.3) in 8 (44%); 5 of 18 (28%) reacted only with La33.3 and with the first 107 N-terminal amino acids (LaA), and 4 (22%) reacted with La33.3, LaA, and the middle region of the La molecule (LaC; amino acids 111–242). One serum reacted with La33.3 and LaC. None of the 18 Precipitin-negative sera was positive on a carboxy-terminal fragment (LaL2/3; amino acids 346–408). In contrast, all 26 Precipitin-positive anti–La/SS-B–positive sera reacted with La33.3, LaA, and LaC, and 92% reacted with LaL2/3. Rheumatoid factor and serum IgG levels were significantly lower in the Precipitin-negative group, providing further evidence of a distinct serologic subset. Conclusion. The restricted epitope recognition by these sera may explain the lack of Precipitin formation and may represent an early autoantibody response to La/SS-B.
Leonard I Bernstein - One of the best experts on this subject based on the ideXlab platform.
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machine operator s lung a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols
Chest, 1995Co-Authors: David I Bernstein, Greg Santilli, James Siskosky, Zana L Lummus, Leonard I BernsteinAbstract:Six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. Two workers also reported fever and weight loss. All six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. Chest radiographs revealed pulmonary interstitial infiltrates in four workers. Lung function tests showed that four workers had decreased diffusing capacity. After removal from the work area, all workers recovered. The metalworking fluid was cultured for bacteria and fungi. Isolates from broth cultures were sonicated to obtain antigen extracts. Serum Precipitins to one or more of the microbial isolates were identified in all six workers but not in eight of nine nonexposed control subjects. The most frequent Precipitin response (six of six workers) was against antigens of Pseudomonas fluorescens, which was cultured from the metalworking fluid. In all workers, Precipitins to at least one other cultured organism were detected; these included Aspergillus niger, Staphylococcus capitas, an acid-fast Rhodococcus sp, and Bacillus pumilus. This represents the first report of hypersensitivity pneumonitis associated with industrial exposure to aerosolized metalworking fluid. Observed Precipitin responses to a variety of microbial contaminants in metalworking fluid strongly suggest a causative role for microbial antigens in the induction and elicitation of this manifestation of hypersensitivity pneumonitis.
John D Reveille - One of the best experts on this subject based on the ideXlab platform.
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contrasting molecular patterns of mhc class ii alleles associated with the anti sm and anti rnp Precipitin autoantibodies in systemic lupus erythematosus
Arthritis & Rheumatism, 1993Co-Authors: Mary L Olsen, Frank C Arnett, John D ReveilleAbstract:Objective. To find evidence of a potential genetic predisposition to the anti-Sm or anti-RNP Precipitin autoantibody responses. Methods. HLA–DR and DQ alleles determined by restriction fragment length polymorphism and/or oligo-typing in 49 subjects with either anti-Sm alone or anti-RNP alone were compared with those in 139 race-matched normal control subjects and 59 race-matched lupus patients without anti-Sm and anti-RNP auto-antibodies. Results. Black patients with anti-Sm Precipitin had increased frequencies of HLA-DR2 and the DQw6-associated DQA1*0102 (P = 0.007, odds ratio [OR] = 6.7) and DQB1*0602 (P = 0.001, OR = 9.1) chain alleles compared with normal black control subjects. Black patients with anti-RNP Precipitin showed significant increases in the DQw5-associated DQA1*0101 (P = 0.03, OR = 5.5) and DQB1*0501 (P = 0.002, OR = 23.3) chain alleles compared with lupus patients without anti-Sm or RNP. White patients with anti-RNP Precipitin showed an increased frequency of the DQw8-associated allele DQB1*0302 (P = 0.02, OR = 3.7) compared with normal controls, as well as an increased frequency of the DQw5-associated alleles DQA1*0101 and DQB1*0501 (P = 0.05, OR = 4.2) compared with lupus patients without anti-Sm or RNP. There were no specific HLA–DR2 or DR4 subtype associations found with either anti-Sm or RNP Precipitin autoantibodies. Conclusion. There are distinct patterns of major histocompatibility complex class II allele associations with the anti-Sm versus the anti-RNP Precipitin autoantibody responses, and HLA–DQ associations may be more primary than HLA–DR associations.
Tom P Gordon - One of the best experts on this subject based on the ideXlab platform.
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restricted epitope recognition by Precipitin negative anti la ss b positive sera
Arthritis & Rheumatism, 1992Co-Authors: Tom P Gordon, Chris Mavrangelos, James MccluskeyAbstract:Objective. To determine whether anti–La/SS-B–positive sera that are Precipitin negative show a distinct B cell epitope pattern. Methods. Serum reactivity was tested with recombinant La/SS-B fusion proteins. Results. Among the 18 Precipitin-negative anti-La/SS-B–positive sera, reactivity was confined to the full-length recombinant protein (La33.3) in 8 (44%); 5 of 18 (28%) reacted only with La33.3 and with the first 107 N-terminal amino acids (LaA), and 4 (22%) reacted with La33.3, LaA, and the middle region of the La molecule (LaC; amino acids 111–242). One serum reacted with La33.3 and LaC. None of the 18 Precipitin-negative sera was positive on a carboxy-terminal fragment (LaL2/3; amino acids 346–408). In contrast, all 26 Precipitin-positive anti–La/SS-B–positive sera reacted with La33.3, LaA, and LaC, and 92% reacted with LaL2/3. Rheumatoid factor and serum IgG levels were significantly lower in the Precipitin-negative group, providing further evidence of a distinct serologic subset. Conclusion. The restricted epitope recognition by these sera may explain the lack of Precipitin formation and may represent an early autoantibody response to La/SS-B.
David I Bernstein - One of the best experts on this subject based on the ideXlab platform.
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machine operator s lung a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols
Chest, 1995Co-Authors: David I Bernstein, Greg Santilli, James Siskosky, Zana L Lummus, Leonard I BernsteinAbstract:Six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. Two workers also reported fever and weight loss. All six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. Chest radiographs revealed pulmonary interstitial infiltrates in four workers. Lung function tests showed that four workers had decreased diffusing capacity. After removal from the work area, all workers recovered. The metalworking fluid was cultured for bacteria and fungi. Isolates from broth cultures were sonicated to obtain antigen extracts. Serum Precipitins to one or more of the microbial isolates were identified in all six workers but not in eight of nine nonexposed control subjects. The most frequent Precipitin response (six of six workers) was against antigens of Pseudomonas fluorescens, which was cultured from the metalworking fluid. In all workers, Precipitins to at least one other cultured organism were detected; these included Aspergillus niger, Staphylococcus capitas, an acid-fast Rhodococcus sp, and Bacillus pumilus. This represents the first report of hypersensitivity pneumonitis associated with industrial exposure to aerosolized metalworking fluid. Observed Precipitin responses to a variety of microbial contaminants in metalworking fluid strongly suggest a causative role for microbial antigens in the induction and elicitation of this manifestation of hypersensitivity pneumonitis.
