The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
António J. A. Nogueira - One of the best experts on this subject based on the ideXlab platform.
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Identification of predator–prey Relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay
Journal of Pest Science, 2009Co-Authors: Sónia A. P. Santos, José A. Pereira, Maria Conceição Rodrigues, Laura M. Torres, Ana Maria N. Pereira, António J. A. NogueiraAbstract:A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae , in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 μg mL^−1. The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive–negative threshold was established at 0.674 μg mL^−1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-os-Montes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Field-collected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and mid-October coinciding with the occurrence of the first, second and third instar nymphs of S. oleae , respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.
Sónia A. P. Santos - One of the best experts on this subject based on the ideXlab platform.
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Identification of predator–prey Relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay
Journal of Pest Science, 2009Co-Authors: Sónia A. P. Santos, José A. Pereira, Maria Conceição Rodrigues, Laura M. Torres, Ana Maria N. Pereira, António J. A. NogueiraAbstract:A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae , in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 μg mL^−1. The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive–negative threshold was established at 0.674 μg mL^−1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-os-Montes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Field-collected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and mid-October coinciding with the occurrence of the first, second and third instar nymphs of S. oleae , respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.
Yanhong Zeng - One of the best experts on this subject based on the ideXlab platform.
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biomagnification of pbdes and alternative brominated flame retardants in a predatory fish using fatty acid signature as a primer
Environment International, 2019Co-Authors: Ying Zhang, Jiangping Wu, Sikang Wu, Yanhong ZengAbstract:Abstract Information on biomagnification of alternative brominated flame retardants (ABFRs) is limited and results are inconclusive, due in part to uncertainty in the understanding of predator/prey Relationships. In the present study, a predatory fish, Channa argus, and several forage fish species were obtained from an ABFR contaminated site. The predator/prey Relationships were identified based on fatty acid (FA) signatures in the predator and prey. Biomagnification factors (BMFs) for several ABFRs including decabromodiphenyl ethane (DBDPE), 1,2‑bis(2,4,6‑tribromophenoxy) ethane (BTBPE), hexabromobenzene (HBB), pentabromotoluene (PBT), and pentabromoethylbenzene (PBEB) were estimated based on the identified predator/prey Relationships. The results showed that crucian carp was the main prey of C. argus, contributing to 71%–100% to its total diet. The mean BMFs for DBDPE, BTBPE, and HBB were 0.06, 0.40, and 0.91, respectively, indicating trophic dilution of these ABFRs. However, biomagnification of PBT and PBEB, with BMFs of 2.09 and 2.13, respectively, was observed. The BMFs for PBT, PBEB and HBB were comparable to or even higher than those for some polybrominated diphenyl ether (PBDE) congeners estimated in the same individual predator, indicating that these emerging pollutants may pose significant environmental risks. The BMFs for ABFRs and PBDEs were significantly and negatively correlated to the log KOWs of these chemicals, suggesting that the biomagnification of these chemicals was depressed due to their superhydrophobic nature.
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biomagnification of pbdes and alternative brominated flame retardants in a predatory fish using fatty acid signature as a primer
Environment International, 2019Co-Authors: Ying Zhang, Jiangping Wu, Sikang Wu, Yanhong ZengAbstract:Abstract Information on biomagnification of alternative brominated flame retardants (ABFRs) is limited and results are inconclusive, due in part to uncertainty in the understanding of predator/prey Relationships. In the present study, a predatory fish, Channa argus, and several forage fish species were obtained from an ABFR contaminated site. The predator/prey Relationships were identified based on fatty acid (FA) signatures in the predator and prey. Biomagnification factors (BMFs) for several ABFRs including decabromodiphenyl ethane (DBDPE), 1,2‑bis(2,4,6‑tribromophenoxy) ethane (BTBPE), hexabromobenzene (HBB), pentabromotoluene (PBT), and pentabromoethylbenzene (PBEB) were estimated based on the identified predator/prey Relationships. The results showed that crucian carp was the main prey of C. argus, contributing to 71%–100% to its total diet. The mean BMFs for DBDPE, BTBPE, and HBB were 0.06, 0.40, and 0.91, respectively, indicating trophic dilution of these ABFRs. However, biomagnification of PBT and PBEB, with BMFs of 2.09 and 2.13, respectively, was observed. The BMFs for PBT, PBEB and HBB were comparable to or even higher than those for some polybrominated diphenyl ether (PBDE) congeners estimated in the same individual predator, indicating that these emerging pollutants may pose significant environmental risks. The BMFs for ABFRs and PBDEs were significantly and negatively correlated to the log KOWs of these chemicals, suggesting that the biomagnification of these chemicals was depressed due to their superhydrophobic nature.
Ana Maria N. Pereira - One of the best experts on this subject based on the ideXlab platform.
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Identification of predator–prey Relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay
Journal of Pest Science, 2009Co-Authors: Sónia A. P. Santos, José A. Pereira, Maria Conceição Rodrigues, Laura M. Torres, Ana Maria N. Pereira, António J. A. NogueiraAbstract:A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae , in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 μg mL^−1. The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive–negative threshold was established at 0.674 μg mL^−1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-os-Montes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Field-collected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and mid-October coinciding with the occurrence of the first, second and third instar nymphs of S. oleae , respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.
Laura M. Torres - One of the best experts on this subject based on the ideXlab platform.
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Identification of predator–prey Relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay
Journal of Pest Science, 2009Co-Authors: Sónia A. P. Santos, José A. Pereira, Maria Conceição Rodrigues, Laura M. Torres, Ana Maria N. Pereira, António J. A. NogueiraAbstract:A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae , in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 μg mL^−1. The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive–negative threshold was established at 0.674 μg mL^−1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-os-Montes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Field-collected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and mid-October coinciding with the occurrence of the first, second and third instar nymphs of S. oleae , respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.