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Gang Hao - One of the best experts on this subject based on the ideXlab platform.

Michał Gleńsk - One of the best experts on this subject based on the ideXlab platform.

  • In Search of High-Yielding and Single-Compound-Yielding Plants: New Sources of Pharmaceutically Important Saponins from the Primulaceae Family.
    Biomolecules, 2020
    Co-Authors: Maciej Włodarczyk, Paweł Pasikowski, Kinga Osiewała, Aleksandra Frankiewicz, Andrzej Dryś, Michał Gleńsk
    Abstract:

    So far, only a few primrose species have been analyzed regarding their saponin composition and content. Moreover, the roots of only two of them are defined by the European Union (EU) Pharmacopoeia monograph and commercially utilized by the pharmaceutical industry. Thus, this study intended to find some new sources of main triterpene saponins from Primulae radix, namely primulasaponins I and II together with the closely related sakurasosaponin. Using isolated standards, UHPLC-ESI-HRMS served to assess over 155 Primulaceae members qualitatively and quantitatively. Nine examples of plants accumulating over 5% of primulasaponin I in their roots were found. Among them, in one case, it was found as the almost sole secondary metabolite with the concentration of 15–20% (Primula grandis L.). A reasonable content of primulasaponin II was found to be typical for Primula vulgaris Huds. and P. megaseifolia Boiss. & Bal. The sakurasosaponin level was found in seven species to exceed 5%. The finding of new, single and rich sources of the abovementioned biomolecules among species that were never analyzed phytochemically is important for future research and economic benefit. The chemotaxonomic significance of the occurrence of these three saponins in Primulaceae is discussed.

  • Lanostane-Type Saponins from Vitaliana primuliflora
    MDPI AG, 2019
    Co-Authors: Maciej Włodarczyk, Antoni Szumny, Michał Gleńsk
    Abstract:

    The phytochemistry of the genera Androsace, Cortusa, Soldanella, and Vitaliana, belonging to the Primulaceae family is not well studied so far. Hence, in this paper, we present the results of UHPLC-MS/MS analysis of several primrose family members as well as isolation and structure determination of two new saponins from Vitaliana primuliflora subsp. praetutiana. These two nor-triterpenoid saponins were characterized as (23S)-17α,23-epoxy-29-hydroxy-3β-[(O-β-d-glucopyranosyl-(1→2)-O-α-l-rhamnopyranosyl-(1→2)-O-β-d-glucopyranosyl-(1→2)-O-α-l-arabinopyranosyl-(1→6)-β-d-glucopyranosyl)oxy]-27-nor-lanost-8-en-25-one and (23S)-17α,23-epoxy-29-hydroxy-3β-[(O-α-l-rhamnopyranosyl-(1→2)-O-β-d-glucopyranosyl-(1→2)-O-α-l-arabinopyranosyl-(1→6)-β-d-glucopyranosyl)oxy]-27-nor-lanost-8-en-25-one, respectively. Their structures were determined by high resolution mass spectrometry (HRMS), tandem mass spectrometry (MS/MS), one- and two-dimensional nuclear magnetic resonance spectroscopy (1D-, and 2D-NMR) analyses. So far, the 27-nor-lanostane monodesmosides were rarely found in dicotyledon plants. Therefore their presence in Vitaliana and also in Androsace species belonging to the Aretia section is unique and reported here for the first time. Additionally, eleven other saponins were determined by HRMS and MS/MS spectra. The isolated lanostane saponins can be considered as chemotaxonomic markers of the family Primulaceae

Arne A. Anderberg - One of the best experts on this subject based on the ideXlab platform.

  • Phylogeny and Biogeography of Dionysia (Primulaceae)
    International Journal of Plant Sciences, 2004
    Co-Authors: Ida Trift, Magnus Lidén, Arne A. Anderberg
    Abstract:

    Cladistic analyses using parsimony jackknifing and Bayesian inference were performed to elucidate phylogenetic relationships in the genus Dionysia. DNA sequences from one nuclear DNA region (ITS) and from two chloroplast genome regions (rps16 intron and trnL‐trnF) were used with and without the addition of a morphological character matrix. A total of 43 species from Primulaceae were sequenced, including 39 species of Dionysia. The results strongly suggest that the sister species to the rest of genus Dionysia are Dionysia balsamea and D. hissarica, not D. mira. No section and subsection with more than one species of Dionysia as currently circumscribed appears to be monophyletic. Instead, species often belong to clades corresponding to geographical proximity. Earlier proposed notions of evolutionary trends and geographical migrations in Dionysia are discussed.

  • The Monophyly of Primula (Primulaceae) Evaluated by Analysis of Sequences from the Chloroplast Gene rbcL
    Systematic Botany, 2002
    Co-Authors: Ida Trift, Mari Källersjö, Arne A. Anderberg
    Abstract:

    Abstract Cladistic analyses using parsimony jackknifing were performed on DNA sequences from the chloroplast gene rbcL to elucidate phylogenetic relationships in Primula and its relationships to other genera of the Primulaceae. A total of 51 species from Primulaceae and 34 species from related families were included. The results showed that the genus Primula was not monophyletic because the genera Dodecatheon, Cortusa, Sredinskya, and Dionysia had their closest relatives within Primula. The results also showed that the genera Pomatosace and Douglasia were derived relatives of Androsace. Diagnostic characters such as leaf vernation, pollen type, and base chromosome numbers were discussed. Communicating Editor: John V. Freudenstein

  • phylogeny of cyclamen l Primulaceae evidence from morphology and sequence data from the internal transcribed spacers of nuclear ribosomal dna
    Plant Systematics and Evolution, 2000
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    A molecular phylogenetic study of the genusCyclamen L. (Primulaceae) has been undertaken, based on sequence data from the 5.8S gene in the ribosomal nuclear DNA and its flanking internal transcribed spacers ITS1 and ITS2. Sequence data from 15 species ofCyclamen and seven outgroup taxa selected from Primulaceae and Myrsinaceae were analyzed phylogenetically. A second analysis based on a combined morphological and molecular dataset was performed to evaluate earlier hypotheses of character evolution in the genus. The results indicate that four monophyletic subgroups may be recognized in the genus, viz.Cyclamen, Psilanthum Schwarz,Eucosme Schwarz andGyrophoebe Schwarz. Each of the four subgenera is diagnosed by distinct basic chromosome number, as well as by morphological and molecular characteristics.

  • Phylogeny ofCyclamen L. (Primulaceae): Evidence from morphology and sequence data from the internal transcribed spacers of nuclear ribosomal DNA
    Plant Systematics and Evolution, 2000
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    A molecular phylogenetic study of the genus Cyclamen L. (Primulaceae) has been undertaken, based on sequence data from the 5.8S gene in the ribosomal nuclear DNA and its flanking internal transcribed spacers ITS1 and ITS2. Sequence data from 15 species of Cyclamen and seven outgroup taxa selected from Primulaceae and Myrsinaceae were analyzed phylogenetically. A second analysis based on a combined morphological and molecular dataset was performed to evaluate earlier hypotheses of character evolution in the genus. The results indicate that four monophyletic subgroups may be recognized in the genus, viz. Cyclamen, Psilanthum Schwarz, Eucosme Schwarz and Gyrophoebe Schwarz. Each of the four subgenera is diagnosed by distinct basic chromosome number, as well as by morphological and molecular characteristics.

  • On the systematic position of the genus Coris (Primulaceae)
    Nordic Journal of Botany, 1998
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    The systematic position of the aberrant primulaceous genus Coris was investigated by means of cladistic analysis of nucleotide sequence data from the chloroplast gene rbcL. One new sequence (Coris) was added to a data matrix composed of a set of previously published sequences. The notion that Coris is most closely related to the Lythraceae is rejected, and very strong support for its position within the Primulaceae was found. The clarincation of this issue is important for future analyses of phylogenetic interrelationships in the Primulales.

Mari Källersjö - One of the best experts on this subject based on the ideXlab platform.

  • The Monophyly of Primula (Primulaceae) Evaluated by Analysis of Sequences from the Chloroplast Gene rbcL
    Systematic Botany, 2002
    Co-Authors: Ida Trift, Mari Källersjö, Arne A. Anderberg
    Abstract:

    Abstract Cladistic analyses using parsimony jackknifing were performed on DNA sequences from the chloroplast gene rbcL to elucidate phylogenetic relationships in Primula and its relationships to other genera of the Primulaceae. A total of 51 species from Primulaceae and 34 species from related families were included. The results showed that the genus Primula was not monophyletic because the genera Dodecatheon, Cortusa, Sredinskya, and Dionysia had their closest relatives within Primula. The results also showed that the genera Pomatosace and Douglasia were derived relatives of Androsace. Diagnostic characters such as leaf vernation, pollen type, and base chromosome numbers were discussed. Communicating Editor: John V. Freudenstein

  • phylogeny of cyclamen l Primulaceae evidence from morphology and sequence data from the internal transcribed spacers of nuclear ribosomal dna
    Plant Systematics and Evolution, 2000
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    A molecular phylogenetic study of the genusCyclamen L. (Primulaceae) has been undertaken, based on sequence data from the 5.8S gene in the ribosomal nuclear DNA and its flanking internal transcribed spacers ITS1 and ITS2. Sequence data from 15 species ofCyclamen and seven outgroup taxa selected from Primulaceae and Myrsinaceae were analyzed phylogenetically. A second analysis based on a combined morphological and molecular dataset was performed to evaluate earlier hypotheses of character evolution in the genus. The results indicate that four monophyletic subgroups may be recognized in the genus, viz.Cyclamen, Psilanthum Schwarz,Eucosme Schwarz andGyrophoebe Schwarz. Each of the four subgenera is diagnosed by distinct basic chromosome number, as well as by morphological and molecular characteristics.

  • Phylogeny ofCyclamen L. (Primulaceae): Evidence from morphology and sequence data from the internal transcribed spacers of nuclear ribosomal DNA
    Plant Systematics and Evolution, 2000
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    A molecular phylogenetic study of the genus Cyclamen L. (Primulaceae) has been undertaken, based on sequence data from the 5.8S gene in the ribosomal nuclear DNA and its flanking internal transcribed spacers ITS1 and ITS2. Sequence data from 15 species of Cyclamen and seven outgroup taxa selected from Primulaceae and Myrsinaceae were analyzed phylogenetically. A second analysis based on a combined morphological and molecular dataset was performed to evaluate earlier hypotheses of character evolution in the genus. The results indicate that four monophyletic subgroups may be recognized in the genus, viz. Cyclamen, Psilanthum Schwarz, Eucosme Schwarz and Gyrophoebe Schwarz. Each of the four subgenera is diagnosed by distinct basic chromosome number, as well as by morphological and molecular characteristics.

  • On the systematic position of the genus Coris (Primulaceae)
    Nordic Journal of Botany, 1998
    Co-Authors: Arne A. Anderberg, Ida Trift, Mari Källersjö
    Abstract:

    The systematic position of the aberrant primulaceous genus Coris was investigated by means of cladistic analysis of nucleotide sequence data from the chloroplast gene rbcL. One new sequence (Coris) was added to a data matrix composed of a set of previously published sequences. The notion that Coris is most closely related to the Lythraceae is rejected, and very strong support for its position within the Primulaceae was found. The clarincation of this issue is important for future analyses of phylogenetic interrelationships in the Primulales.

Fergan Karaer - One of the best experts on this subject based on the ideXlab platform.