The Experts below are selected from a list of 10038 Experts worldwide ranked by ideXlab platform
Narutoshi Hibino - One of the best experts on this subject based on the ideXlab platform.
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mechanical stimulation enhances development of Scaffold free 3d printed engineered heart tissue grafts
Journal of Tissue Engineering and Regenerative Medicine, 2021Co-Authors: Cecillia Lui, Alexander F Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon F Tomaselli, Yun Chen, Narutoshi HibinoAbstract:Current efforts to engineer a clinically relevant tissue graft from human induced pluripotent stem cells (hiPSCs) have relied on the addition or utilization of external Scaffolding material. However any imbalance in the interactions between embedded cells and their surroundings may hinder the success of the resulting tissue graft. Therefore the goal of our study was to create Scaffold-free 3D-printed cardiac tissue grafts from hiPSC-derived cardiomyocytes (CMs), and to evaluate whether or not mechanical stimulation would result in improved graft maturation. To explore this, we used a 3D bioprinter to Produce Scaffold-free cardiac tissue grafts from hiPSC-derived CM cell spheroids. Static mechanical stretching of these grafts significantly increased sarcomere length compared to unstimulated free-floating tissues, as determined by immunofluorescent image analysis. Stretched tissue was found to have decreased elastic modulus, increased maximal contractile force, and increased alignment of formed extracellular matrix, as expected in a functionally maturing tissue graft. Additionally, stretched tissues had upregulated expression of cardiac-specific gene transcripts, consistent with increased cardiac-like cellular identity. Finally, analysis of extracellular matrix organization in stretched grafts suggests improved remodeling by embedded cardiac fibroblasts. Taken together, our results suggest that mechanical stretching stimulates hiPSC derived-CMs in a 3D-printed Scaffold-free tissue graft to develop mature cardiac material structuring and cellular fates. Our work highlights the critical role of mechanical conditioning as an important engineering strategy toward developing clinically applicable, Scaffold-free human cardiac tissue grafts. This article is protected by copyright. All rights reserved.
Gordon F Tomaselli - One of the best experts on this subject based on the ideXlab platform.
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mechanical stimulation enhances development of Scaffold free 3d printed engineered heart tissue grafts
Journal of Tissue Engineering and Regenerative Medicine, 2021Co-Authors: Cecillia Lui, Alexander F Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon F Tomaselli, Yun Chen, Narutoshi HibinoAbstract:Current efforts to engineer a clinically relevant tissue graft from human induced pluripotent stem cells (hiPSCs) have relied on the addition or utilization of external Scaffolding material. However any imbalance in the interactions between embedded cells and their surroundings may hinder the success of the resulting tissue graft. Therefore the goal of our study was to create Scaffold-free 3D-printed cardiac tissue grafts from hiPSC-derived cardiomyocytes (CMs), and to evaluate whether or not mechanical stimulation would result in improved graft maturation. To explore this, we used a 3D bioprinter to Produce Scaffold-free cardiac tissue grafts from hiPSC-derived CM cell spheroids. Static mechanical stretching of these grafts significantly increased sarcomere length compared to unstimulated free-floating tissues, as determined by immunofluorescent image analysis. Stretched tissue was found to have decreased elastic modulus, increased maximal contractile force, and increased alignment of formed extracellular matrix, as expected in a functionally maturing tissue graft. Additionally, stretched tissues had upregulated expression of cardiac-specific gene transcripts, consistent with increased cardiac-like cellular identity. Finally, analysis of extracellular matrix organization in stretched grafts suggests improved remodeling by embedded cardiac fibroblasts. Taken together, our results suggest that mechanical stretching stimulates hiPSC derived-CMs in a 3D-printed Scaffold-free tissue graft to develop mature cardiac material structuring and cellular fates. Our work highlights the critical role of mechanical conditioning as an important engineering strategy toward developing clinically applicable, Scaffold-free human cardiac tissue grafts. This article is protected by copyright. All rights reserved.
Cecillia Lui - One of the best experts on this subject based on the ideXlab platform.
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mechanical stimulation enhances development of Scaffold free 3d printed engineered heart tissue grafts
Journal of Tissue Engineering and Regenerative Medicine, 2021Co-Authors: Cecillia Lui, Alexander F Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon F Tomaselli, Yun Chen, Narutoshi HibinoAbstract:Current efforts to engineer a clinically relevant tissue graft from human induced pluripotent stem cells (hiPSCs) have relied on the addition or utilization of external Scaffolding material. However any imbalance in the interactions between embedded cells and their surroundings may hinder the success of the resulting tissue graft. Therefore the goal of our study was to create Scaffold-free 3D-printed cardiac tissue grafts from hiPSC-derived cardiomyocytes (CMs), and to evaluate whether or not mechanical stimulation would result in improved graft maturation. To explore this, we used a 3D bioprinter to Produce Scaffold-free cardiac tissue grafts from hiPSC-derived CM cell spheroids. Static mechanical stretching of these grafts significantly increased sarcomere length compared to unstimulated free-floating tissues, as determined by immunofluorescent image analysis. Stretched tissue was found to have decreased elastic modulus, increased maximal contractile force, and increased alignment of formed extracellular matrix, as expected in a functionally maturing tissue graft. Additionally, stretched tissues had upregulated expression of cardiac-specific gene transcripts, consistent with increased cardiac-like cellular identity. Finally, analysis of extracellular matrix organization in stretched grafts suggests improved remodeling by embedded cardiac fibroblasts. Taken together, our results suggest that mechanical stretching stimulates hiPSC derived-CMs in a 3D-printed Scaffold-free tissue graft to develop mature cardiac material structuring and cellular fates. Our work highlights the critical role of mechanical conditioning as an important engineering strategy toward developing clinically applicable, Scaffold-free human cardiac tissue grafts. This article is protected by copyright. All rights reserved.
Alexander F Chin - One of the best experts on this subject based on the ideXlab platform.
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mechanical stimulation enhances development of Scaffold free 3d printed engineered heart tissue grafts
Journal of Tissue Engineering and Regenerative Medicine, 2021Co-Authors: Cecillia Lui, Alexander F Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon F Tomaselli, Yun Chen, Narutoshi HibinoAbstract:Current efforts to engineer a clinically relevant tissue graft from human induced pluripotent stem cells (hiPSCs) have relied on the addition or utilization of external Scaffolding material. However any imbalance in the interactions between embedded cells and their surroundings may hinder the success of the resulting tissue graft. Therefore the goal of our study was to create Scaffold-free 3D-printed cardiac tissue grafts from hiPSC-derived cardiomyocytes (CMs), and to evaluate whether or not mechanical stimulation would result in improved graft maturation. To explore this, we used a 3D bioprinter to Produce Scaffold-free cardiac tissue grafts from hiPSC-derived CM cell spheroids. Static mechanical stretching of these grafts significantly increased sarcomere length compared to unstimulated free-floating tissues, as determined by immunofluorescent image analysis. Stretched tissue was found to have decreased elastic modulus, increased maximal contractile force, and increased alignment of formed extracellular matrix, as expected in a functionally maturing tissue graft. Additionally, stretched tissues had upregulated expression of cardiac-specific gene transcripts, consistent with increased cardiac-like cellular identity. Finally, analysis of extracellular matrix organization in stretched grafts suggests improved remodeling by embedded cardiac fibroblasts. Taken together, our results suggest that mechanical stretching stimulates hiPSC derived-CMs in a 3D-printed Scaffold-free tissue graft to develop mature cardiac material structuring and cellular fates. Our work highlights the critical role of mechanical conditioning as an important engineering strategy toward developing clinically applicable, Scaffold-free human cardiac tissue grafts. This article is protected by copyright. All rights reserved.
Seungman Park - One of the best experts on this subject based on the ideXlab platform.
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mechanical stimulation enhances development of Scaffold free 3d printed engineered heart tissue grafts
Journal of Tissue Engineering and Regenerative Medicine, 2021Co-Authors: Cecillia Lui, Alexander F Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon F Tomaselli, Yun Chen, Narutoshi HibinoAbstract:Current efforts to engineer a clinically relevant tissue graft from human induced pluripotent stem cells (hiPSCs) have relied on the addition or utilization of external Scaffolding material. However any imbalance in the interactions between embedded cells and their surroundings may hinder the success of the resulting tissue graft. Therefore the goal of our study was to create Scaffold-free 3D-printed cardiac tissue grafts from hiPSC-derived cardiomyocytes (CMs), and to evaluate whether or not mechanical stimulation would result in improved graft maturation. To explore this, we used a 3D bioprinter to Produce Scaffold-free cardiac tissue grafts from hiPSC-derived CM cell spheroids. Static mechanical stretching of these grafts significantly increased sarcomere length compared to unstimulated free-floating tissues, as determined by immunofluorescent image analysis. Stretched tissue was found to have decreased elastic modulus, increased maximal contractile force, and increased alignment of formed extracellular matrix, as expected in a functionally maturing tissue graft. Additionally, stretched tissues had upregulated expression of cardiac-specific gene transcripts, consistent with increased cardiac-like cellular identity. Finally, analysis of extracellular matrix organization in stretched grafts suggests improved remodeling by embedded cardiac fibroblasts. Taken together, our results suggest that mechanical stretching stimulates hiPSC derived-CMs in a 3D-printed Scaffold-free tissue graft to develop mature cardiac material structuring and cellular fates. Our work highlights the critical role of mechanical conditioning as an important engineering strategy toward developing clinically applicable, Scaffold-free human cardiac tissue grafts. This article is protected by copyright. All rights reserved.