The Experts below are selected from a list of 27354 Experts worldwide ranked by ideXlab platform
Daniel Eikel - One of the best experts on this subject based on the ideXlab platform.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Carol Aso, Suzie Yeh, Walte A Korfmache, Jack HenioAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections. Copyright © 2011 John Wiley & Sons, Ltd.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Suzie Yeh, Carol Bason, Walter A Korfmacher, Jack HenionAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections.
Jack Henion - One of the best experts on this subject based on the ideXlab platform.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Suzie Yeh, Carol Bason, Walter A Korfmacher, Jack HenionAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections.
Jack Henio - One of the best experts on this subject based on the ideXlab platform.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Carol Aso, Suzie Yeh, Walte A Korfmache, Jack HenioAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections. Copyright © 2011 John Wiley & Sons, Ltd.
Suzie Yeh - One of the best experts on this subject based on the ideXlab platform.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Carol Aso, Suzie Yeh, Walte A Korfmache, Jack HenioAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections. Copyright © 2011 John Wiley & Sons, Ltd.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Suzie Yeh, Carol Bason, Walter A Korfmacher, Jack HenionAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections.
Sheri Smith - One of the best experts on this subject based on the ideXlab platform.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Carol Aso, Suzie Yeh, Walte A Korfmache, Jack HenioAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections. Copyright © 2011 John Wiley & Sons, Ltd.
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liquid extraction surface analysis mass spectrometry lesa ms as a novel Profiling Tool for drug distribution and metabolism analysis the terfenadine example
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Daniel Eikel, Marissa Vavrek, Sheri Smith, Suzie Yeh, Carol Bason, Walter A Korfmacher, Jack HenionAbstract:Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel surface Profiling technique that combines micro-liquid extraction from a solid surface with nano-electrospray mass spectrometry. One potential application is the examination of the distribution of drugs and their metabolites by analyzing ex vivo tissue sections, an area where quantitative whole body autoradiography (QWBA) is traditionally employed. However, QWBA relies on the use of radiolabeled drugs and is limited to total radioactivity measured whereas LESA-MS can provide drug- and metabolite-specific distribution information. Here, we evaluate LESA-MS, examining the distribution and biotransformation of unlabeled terfenadine in mice and compare our findings to QWBA, whole tissue LC/MS/MS and MALDI-MSI. The spatial resolution of LESA-MS can be optimized to ca. 1 mm on tissues such as brain, liver and kidney, also enabling drug Profiling within a single organ. LESA-MS can readily identify the biotransformation of terfenadine to its major, active metabolite fexofenadine. Relative quantification can confirm the rapid absorption of terfendine after oral dosage, its extensive first pass metabolism and the distribution of both compounds into systemic tissues such as muscle, spleen and kidney. The elimination appears to be consistent with biliary excretion and only trace levels of fexofenadine could be confirmed in brain. We found LESA-MS to be more informative in terms of drug distribution than a comparable MALDI-MS imaging study, likely due to its favorable overall sensitivity due to the larger surface area sampled. LESA-MS appears to be a useful new Profiling Tool for examining the distribution of drugs and their metabolites in tissue sections.
