The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Bénédicte Ménez - One of the best experts on this subject based on the ideXlab platform.
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Potential of Cathodoluminescence Microscopy and Spectroscopy for the Detection of Prokaryotic Cells on Minerals
Astrobiology, 2010Co-Authors: Céline Rommevaux-jestin, Bénédicte MénezAbstract:Abstract Detecting mineral-hosted ecosystems to assess the extent and functioning of the biosphere from the surface to deep Earth requires appropriate techniques that provide, beyond the morphological criteria, indubitable clues of the presence of Prokaryotic Cells. Here, we evaluate the capability of cathodoluminescence microscopy and spectroscopy, implemented on a scanning electron microscope, to identify prokaryotes on mineral surfaces. For this purpose, we used, as a first step, a simple model of either unstained or stained cultivable Cells (Escherichia coli, Deinococcus radiodurans) deposited on minerals that are common in the oceanic crust (basaltic glass, amphibole, pyroxene, and magnetite). Our results demonstrate that the detection of Cells is possible at the micrometric level on the investigated minerals through the intrinsic fluorescence of their constituting macromolecules (aromatic amino and nucleic acids, coenzymes). This allows us to distinguish biomorph inorganic phases from Cells. This ea...
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:he involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:International audiencehe involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere
Emmanuelle Gérard - One of the best experts on this subject based on the ideXlab platform.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:he involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:International audiencehe involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere
Céline Rommevaux-jestin - One of the best experts on this subject based on the ideXlab platform.
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Potential of Cathodoluminescence Microscopy and Spectroscopy for the Detection of Prokaryotic Cells on Minerals
Astrobiology, 2010Co-Authors: Céline Rommevaux-jestin, Bénédicte MénezAbstract:Abstract Detecting mineral-hosted ecosystems to assess the extent and functioning of the biosphere from the surface to deep Earth requires appropriate techniques that provide, beyond the morphological criteria, indubitable clues of the presence of Prokaryotic Cells. Here, we evaluate the capability of cathodoluminescence microscopy and spectroscopy, implemented on a scanning electron microscope, to identify prokaryotes on mineral surfaces. For this purpose, we used, as a first step, a simple model of either unstained or stained cultivable Cells (Escherichia coli, Deinococcus radiodurans) deposited on minerals that are common in the oceanic crust (basaltic glass, amphibole, pyroxene, and magnetite). Our results demonstrate that the detection of Cells is possible at the micrometric level on the investigated minerals through the intrinsic fluorescence of their constituting macromolecules (aromatic amino and nucleic acids, coenzymes). This allows us to distinguish biomorph inorganic phases from Cells. This ea...
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:he involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:International audiencehe involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere
Alain Bonneville - One of the best experts on this subject based on the ideXlab platform.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:he involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:International audiencehe involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere
Yuheng Wang - One of the best experts on this subject based on the ideXlab platform.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:he involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere.
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Detection and phylogenetic identification of labeled Prokaryotic Cells on mineral surfaces using Scanning X-ray Microscopy
Chemical Geology, 2007Co-Authors: Bénédicte Ménez, Céline Rommevaux-jestin, Murielle Salomé, Yuheng Wang, Pascal Philippot, Alain Bonneville, Emmanuelle GérardAbstract:International audiencehe involvement of intraterrestrial microbes in geochemical cycles is now well recognized. However, owing to the small number of appropriate methods for probing these ecosystems, the exploration of their metabolic diversity, energy sources, and biogeochemical transformations remains limited. Here we demonstrate the ability of scanning X-ray microscopy using synchrotron radiation to localize and characterize the phylogenetic affiliation of individual Prokaryotic Cells on various mineral surfaces (e.g. carbonates, basaltic glass) when combined with a newly developed protocol based on fluorescence in situ hybridization coupled to ultra-small immunogold. The possibility to associate simultaneously the phylogenetic identification of microorganisms with the chemical and structural characteristics of associated mineral phases (i.e. inorganic substrate and biomineralizations), offers great interest for assessing the geochemical impact of subsurface microbial communities and unraveling microbe-mineral interactions in the deep biosphere
