The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
L. I. Terrazas-vldés - One of the best experts on this subject based on the ideXlab platform.
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The descrease of thein vitro Proliferative Response of zinc-treated stressed mice’s thymic lymphocytes
InflammoPharmacology, 1999Co-Authors: F. García-tamayo, N. Malpica López, M. Aguirre, L. I. Terrazas-vldésAbstract:Prolonged stimulation of newborn mice by intraperitoneal injections with inactivated staphylococci induces a chronic neonatal inflammatory reaction and an associated oxidative-stress Response. The chronically stimulated animals exhibit anorexy. show a reduction in their body weight and undergo a depression in both antibody synthesis and in vitro proliferativc Response of Con A-stimulated splenic T-lymphocytes. These stressed animals also develop adrenal hyperplasia, hypozincamia and thymic hypoplasia. Despite this stress-mediated thymic involution, Con-A stimulated T-lymphocytes from thymus displayed increased their in vitro Proliferative Response. Results of the present work show that intramuscular injections of zinc acetate in stressed mice, one single dose (5 μg) every other day for two weeks, reduce both the zinc concentration in the thymus gland and the in vitro Proliferative Response of their Con A-stimulated T-lymphocytes. The results suggest that prophylactic administration of zinc can have benefical consequences on the immunity of chronically stressed mice.
F. García-tamayo - One of the best experts on this subject based on the ideXlab platform.
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The descrease of thein vitro Proliferative Response of zinc-treated stressed mice’s thymic lymphocytes
InflammoPharmacology, 1999Co-Authors: F. García-tamayo, N. Malpica López, M. Aguirre, L. I. Terrazas-vldésAbstract:Prolonged stimulation of newborn mice by intraperitoneal injections with inactivated staphylococci induces a chronic neonatal inflammatory reaction and an associated oxidative-stress Response. The chronically stimulated animals exhibit anorexy. show a reduction in their body weight and undergo a depression in both antibody synthesis and in vitro proliferativc Response of Con A-stimulated splenic T-lymphocytes. These stressed animals also develop adrenal hyperplasia, hypozincamia and thymic hypoplasia. Despite this stress-mediated thymic involution, Con-A stimulated T-lymphocytes from thymus displayed increased their in vitro Proliferative Response. Results of the present work show that intramuscular injections of zinc acetate in stressed mice, one single dose (5 μg) every other day for two weeks, reduce both the zinc concentration in the thymus gland and the in vitro Proliferative Response of their Con A-stimulated T-lymphocytes. The results suggest that prophylactic administration of zinc can have benefical consequences on the immunity of chronically stressed mice.
Elisabetta Vegeto - One of the best experts on this subject based on the ideXlab platform.
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Selective Proliferative Response of microglia to alternative polarization signals
Journal of Neuroinflammation, 2017Co-Authors: Giovanna Pepe, Marcella De Maglie, Lucia Minoli, Alessandro Villa, Adriana Maggi, Elisabetta VegetoAbstract:Background Microglia are resident myeloid cells of the central nervous system (CNS) that are maintained by self-renewal and actively participate in tissue homeostasis and immune defense. Under the influence of endogenous or pathological signals, microglia undertake biochemical transformations that are schematically classified as the pro-inflammatory M1 phenotype and the alternatively activated M2 state. Dysregulated proliferation of M1-activated microglia has detrimental effects, while an increased number of microglia with the alternative, pro-resolving phenotype might be beneficial in brain pathologies; however, the Proliferative Response of microglia to M2 signals is not yet known. We thus evaluated the ability of interleukin-4 (IL-4), a typical M2 and Proliferative signal for peripheral macrophages, to induce microglia proliferation and compared it with other Proliferative and M2 polarizing stimuli for macrophages, namely colony-stimulating factor-1 (CSF-1) and the estrogen hormone, 17β-estradiol (E_2). Methods Recombinant IL-4 was delivered to the brain of adult mice by intracerebroventricular (i.c.v.) injection; whole brain areas or ex vivo-sorted microglia were analyzed by real-time PCR for assessing the mRNA levels of genes related with cell proliferation ( Ki67 , CDK-1 , and CcnB2 ) and M2 polarization ( Arg1 , Fizz1 , Ym-1 ) or by FACS analyses of in vivo BrdU incorporation in microglia. Primary cultures of microglia and astrocytes were also tested for Proliferative effects. Results Our results show that IL-4 only slightly modified the expression of cell cycle-related genes in some brain areas but not in microglia, where it strongly enhanced M2 gene expression; on the contrary, brain delivery of CSF-1 triggered proliferation as well as M2 polarization of microglia both in vivo and in vitro. Similar to IL-4, the systemic E_2 administration failed to induce microglia proliferation while it increased M2 gene expression. Conclusions Our data show that, in contrast to the wider responsiveness of peripheral macrophages, microglia proliferation is stimulated by selected M2 polarizing stimuli suggesting a role for the local microenvironment and developmental origin of tissue macrophages in regulating self-renewal following alternative activating stimuli.
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selective Proliferative Response of microglia to alternative polarization signals
Journal of Neuroinflammation, 2017Co-Authors: Giovanna Pepe, Marcella De Maglie, Lucia Minoli, Alessandro Villa, Adriana Maggi, Elisabetta VegetoAbstract:Microglia are resident myeloid cells of the central nervous system (CNS) that are maintained by self-renewal and actively participate in tissue homeostasis and immune defense. Under the influence of endogenous or pathological signals, microglia undertake biochemical transformations that are schematically classified as the pro-inflammatory M1 phenotype and the alternatively activated M2 state. Dysregulated proliferation of M1-activated microglia has detrimental effects, while an increased number of microglia with the alternative, pro-resolving phenotype might be beneficial in brain pathologies; however, the Proliferative Response of microglia to M2 signals is not yet known. We thus evaluated the ability of interleukin-4 (IL-4), a typical M2 and Proliferative signal for peripheral macrophages, to induce microglia proliferation and compared it with other Proliferative and M2 polarizing stimuli for macrophages, namely colony-stimulating factor-1 (CSF-1) and the estrogen hormone, 17β-estradiol (E2). Recombinant IL-4 was delivered to the brain of adult mice by intracerebroventricular (i.c.v.) injection; whole brain areas or ex vivo-sorted microglia were analyzed by real-time PCR for assessing the mRNA levels of genes related with cell proliferation (Ki67, CDK-1, and CcnB2) and M2 polarization (Arg1, Fizz1, Ym-1) or by FACS analyses of in vivo BrdU incorporation in microglia. Primary cultures of microglia and astrocytes were also tested for Proliferative effects. Our results show that IL-4 only slightly modified the expression of cell cycle-related genes in some brain areas but not in microglia, where it strongly enhanced M2 gene expression; on the contrary, brain delivery of CSF-1 triggered proliferation as well as M2 polarization of microglia both in vivo and in vitro. Similar to IL-4, the systemic E2 administration failed to induce microglia proliferation while it increased M2 gene expression. Our data show that, in contrast to the wider responsiveness of peripheral macrophages, microglia proliferation is stimulated by selected M2 polarizing stimuli suggesting a role for the local microenvironment and developmental origin of tissue macrophages in regulating self-renewal following alternative activating stimuli.
M. Aguirre - One of the best experts on this subject based on the ideXlab platform.
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The descrease of thein vitro Proliferative Response of zinc-treated stressed mice’s thymic lymphocytes
InflammoPharmacology, 1999Co-Authors: F. García-tamayo, N. Malpica López, M. Aguirre, L. I. Terrazas-vldésAbstract:Prolonged stimulation of newborn mice by intraperitoneal injections with inactivated staphylococci induces a chronic neonatal inflammatory reaction and an associated oxidative-stress Response. The chronically stimulated animals exhibit anorexy. show a reduction in their body weight and undergo a depression in both antibody synthesis and in vitro proliferativc Response of Con A-stimulated splenic T-lymphocytes. These stressed animals also develop adrenal hyperplasia, hypozincamia and thymic hypoplasia. Despite this stress-mediated thymic involution, Con-A stimulated T-lymphocytes from thymus displayed increased their in vitro Proliferative Response. Results of the present work show that intramuscular injections of zinc acetate in stressed mice, one single dose (5 μg) every other day for two weeks, reduce both the zinc concentration in the thymus gland and the in vitro Proliferative Response of their Con A-stimulated T-lymphocytes. The results suggest that prophylactic administration of zinc can have benefical consequences on the immunity of chronically stressed mice.
N. Malpica López - One of the best experts on this subject based on the ideXlab platform.
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The descrease of thein vitro Proliferative Response of zinc-treated stressed mice’s thymic lymphocytes
InflammoPharmacology, 1999Co-Authors: F. García-tamayo, N. Malpica López, M. Aguirre, L. I. Terrazas-vldésAbstract:Prolonged stimulation of newborn mice by intraperitoneal injections with inactivated staphylococci induces a chronic neonatal inflammatory reaction and an associated oxidative-stress Response. The chronically stimulated animals exhibit anorexy. show a reduction in their body weight and undergo a depression in both antibody synthesis and in vitro proliferativc Response of Con A-stimulated splenic T-lymphocytes. These stressed animals also develop adrenal hyperplasia, hypozincamia and thymic hypoplasia. Despite this stress-mediated thymic involution, Con-A stimulated T-lymphocytes from thymus displayed increased their in vitro Proliferative Response. Results of the present work show that intramuscular injections of zinc acetate in stressed mice, one single dose (5 μg) every other day for two weeks, reduce both the zinc concentration in the thymus gland and the in vitro Proliferative Response of their Con A-stimulated T-lymphocytes. The results suggest that prophylactic administration of zinc can have benefical consequences on the immunity of chronically stressed mice.
