The Experts below are selected from a list of 204 Experts worldwide ranked by ideXlab platform
Sergei A Eremin - One of the best experts on this subject based on the ideXlab platform.
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A quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent, reverse micelles of AOT inn-octane: Effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system, n -octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degree W _0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10 nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
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a quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent reverse micelles of aot in n octane effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system,n-octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degreeW 0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
E. G. Matveeva - One of the best experts on this subject based on the ideXlab platform.
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A quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent, reverse micelles of AOT inn-octane: Effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system, n -octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degree W _0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10 nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
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a quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent reverse micelles of aot in n octane effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system,n-octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degreeW 0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
Stephen H Curry - One of the best experts on this subject based on the ideXlab platform.
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Pharmacokinetics of Promazine in patients with hepatic cirrhosis—correlation with a novel galactose single point method
Journal of Pharmaceutical Sciences, 2016Co-Authors: Oliver Yoa Pu Hu, Tuzenyen Y Sheeng, Hung‐shang ‐s Tang, Tung‐chao ‐c Chen, Stephen H CurryAbstract:We examined Promazine pharmacokinetics in nine patients with hepatic cirrhosis and in six healthy subjects. A specific and sensitive HPLC method was used to measure Promazine concentrations in plasma, plasma water (free drug), red blood cells, and urine after oral administration of Promazine (2 × 50 mg tablet). There were highly significant reductions in total plasma clearance (p < 0.01), free drug total plasma clearance (p < 0.01), metabolic clearance (p < 0.01), metabolic clearance of free drug (p < 0.01), and fraction bound (p < 0.01) in the cirrhotic patients. The elimination half‐life and the area under the plasma concentration–time curve were significantly increased (p < 0.001 and p < 0.05, respectively) in the cirrhotic patients. However, the overall excreted Promazine in urine, time to the Promazine peak concentration, distribution half‐life, renal clearance, apparent volume of distribution, and the Promazine concentration ratio between plasma and red blood cells were not different. Thus caution is needed in using Promazine for patients with hepatic cirrhosis. A newly developed galactose single point (GSP) method was applied to quantitatively measure the residual liver function in cirrhosis patients and successfully correlated it with Promazine elimination half‐life (r = 0.770, p < 0.01), total plasma clearance of free drug (r = 0.899, p < 0.005), metabolic clearance of free drug (r 0.902, p < 0.005), and plasma protein binding (r = 0.822, p < 0.005). GSP may be a convenient index for Promazine routine dosage adjustment in patients with liver cirrhosis. Further studies are needed for developing GSP in to a routine index for drug dosage adjustment in metabolically impaired patients.
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pharmacokinetics of Promazine in patients with hepatic cirrhosis correlation with a novel galactose single point method
Journal of Pharmaceutical Sciences, 1995Co-Authors: Oliver Yoa Pu Hu, Hungshang S Tang, Tuzenyen Y Sheeng, Tungchao C Chen, Stephen H CurryAbstract:We examined Promazine pharmacokinetics in nine patients with hepatic cirrhosis and in six healthy subjects. A specific and sensitive HPLC method was used to measure Promazine concentrations in plasma, plasma water (free drug), red blood cells, and urine after oral administration of Promazine (2 × 50 mg tablet). There were highly significant reductions in total plasma clearance (p < 0.01), free drug total plasma clearance (p < 0.01), metabolic clearance (p < 0.01), metabolic clearance of free drug (p < 0.01), and fraction bound (p < 0.01) in the cirrhotic patients. The elimination half‐life and the area under the plasma concentration–time curve were significantly increased (p < 0.001 and p < 0.05, respectively) in the cirrhotic patients. However, the overall excreted Promazine in urine, time to the Promazine peak concentration, distribution half‐life, renal clearance, apparent volume of distribution, and the Promazine concentration ratio between plasma and red blood cells were not different. Thus caution is needed in using Promazine for patients with hepatic cirrhosis. A newly developed galactose single point (GSP) method was applied to quantitatively measure the residual liver function in cirrhosis patients and successfully correlated it with Promazine elimination half‐life (r = 0.770, p < 0.01), total plasma clearance of free drug (r = 0.899, p < 0.005), metabolic clearance of free drug (r 0.902, p < 0.005), and plasma protein binding (r = 0.822, p < 0.005). GSP may be a convenient index for Promazine routine dosage adjustment in patients with liver cirrhosis. Further studies are needed for developing GSP in to a routine index for drug dosage adjustment in metabolically impaired patients.
Jeanne V. Samsonova - One of the best experts on this subject based on the ideXlab platform.
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A quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent, reverse micelles of AOT inn-octane: Effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system, n -octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degree W _0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10 nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
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a quenching fluoroimmunoassay for analysis of the pesticide propazine in an apolar organic solvent reverse micelles of aot in n octane effect of the micellar matrix and labeled antigen structure
Journal of Fluorescence, 1997Co-Authors: E. G. Matveeva, Jeanne V. Samsonova, Sergei A EreminAbstract:A simple way of directly observing antigen-antibody binding in a reverse micellar system,n-octane containing reverse micelles of aerosol OT (AOT), using the hydrophobic pesticide propazine as antigen, is described. We observed two processes during fluorescein-labeled propazine (FP)-antibody (Ab) interaction in reverse micelles: (1) quenching of the fluorescence of FP after mixing of Ab and FP (due immune complex formation) and (2) restoration of FP fluorescence after addition of excess propazine to the immune complex formed. We found that the quenching efficiency depends on both the properties of the reverse micellar system (surfactant concentration, hydration degreeW 0 = [water]/[surfactant]) and the structure of the labeled antigen. A quenching fluoroimmunoassay of propazine both in apolar organic solvents and in water is developed. The method is homogeneous. The quenching time is 10–30 min, and the detection limit of propazine is 100 nM (20 Μg/L) in organic solvent and 10nM (2 Μg/L) in water. Propazine can be added to the reverse micellar system when dissolved in AOT/octane, or in an octane/chloroform mixture, or in chloroform. This makes possible the use of the analysis directly for pesticide extracts in nonpolar organic solvents.
L M Yudi - One of the best experts on this subject based on the ideXlab platform.
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Promazine π mers formation at a 1 2 dichloroethane water interface
Electrochimica Acta, 2008Co-Authors: Lorena M A Monzon, L M YudiAbstract:Abstract The behavior of Promazine in 1,2-dichloroethane was studied using cyclic voltammetry at the ITIES and UV–vis spectrophotometry. The analysis of voltammograms and spectra obtained varying Promazine concentration, pH, nature and concentration of organic electrolyte and applying positive polarisation at the interface allowed us to postulate a reaction scheme consisting in a first step of Promazine partition to organic phase, followed by a charge transfer complex (CTC) formation with 1,2-dichloroethane. This CTC induces the oxidation of Promazine to the corresponding radical cation which is stabilised in organic phase by π-mers formation. π-mer complexes form ion pairs with the anions of the organic base electrolyte. Similar results were found using methotrimeprazine, another phenothiazine derivative, with a methoxyl group attached to the ring. ChlorPromazine, trifluPromazine, perphenazine and fluphenazine were stable in 1,2-dichloroethane.
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Promazine π-mers formation at a 1,2-dichloroethane/water interface
Electrochimica Acta, 2007Co-Authors: Lorena M A Monzon, L M YudiAbstract:Abstract The behavior of Promazine in 1,2-dichloroethane was studied using cyclic voltammetry at the ITIES and UV–vis spectrophotometry. The analysis of voltammograms and spectra obtained varying Promazine concentration, pH, nature and concentration of organic electrolyte and applying positive polarisation at the interface allowed us to postulate a reaction scheme consisting in a first step of Promazine partition to organic phase, followed by a charge transfer complex (CTC) formation with 1,2-dichloroethane. This CTC induces the oxidation of Promazine to the corresponding radical cation which is stabilised in organic phase by π-mers formation. π-mer complexes form ion pairs with the anions of the organic base electrolyte. Similar results were found using methotrimeprazine, another phenothiazine derivative, with a methoxyl group attached to the ring. ChlorPromazine, trifluPromazine, perphenazine and fluphenazine were stable in 1,2-dichloroethane.
