The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform
Chiaohsiung Chuang - One of the best experts on this subject based on the ideXlab platform.
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a defoaming agent should be used with Pronase premedication to improve visibility in upper gastrointestinal endoscopy
Endoscopy, 2002Co-Authors: Borshyang Sheu, C H Wu, Chiaohsiung ChuangAbstract:BACKGROUND AND STUDY AIMS: The study tested whether Pronase can improve endoscopic visibility and alter the accuracy of the CLO test for H. pylori detection. PATIENTS AND METHODS: A total of 160 patients were randomly assigned to receive one of five premedications for endoscopy: group A: dimethylpolysiloxane (DMPS) alone; group B: DMPS plus water (up to 100 ml); group C: Pronase only, with 100 ml water; group D: Pronase and sodium bicarbonate plus water up to 100 ml; group E: Pronase, sodium bicarbonate, and DMPS, plus water up to 100 ml. Endoscopists, who were unaware of the premedication method administered, assessed visibility scores (range 1 - 4) for the antrum, lower gastric body, upper gastric body, and fundus. The higher the score, the less clear the visibility. The sum of scores from the four locations was defined as the total visibility score. A CLO test was also done during the endoscopy. One week after their endoscopy, patients in groups C, D, and E were scheduled for a (13)C-urea breath test (UBT). RESULTS: Group E patients had a significantly lower total visibility score than those in the other four groups ( P < 0.05). Groups C and D had higher total visibility scores than the other three groups ( P < 0.05). The scores did not significantly differ between groups A and B. Based on the UBT results, the sensitivity and specificity of the CLO test were 92.6 % and 96.2 %, respectively. CONCLUSIONS: Premedication as in group E provided the clearest endoscopic visibility. Without the application of DMPS, Pronase alone cannot improve endoscopic visibility. Pronase does not influence H. pylori identification using the CLO test.
Masahiro Asaka - One of the best experts on this subject based on the ideXlab platform.
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removal of mucus for ultrastructural observation of the surface of human gastric epithelium using Pronase
Helicobacter, 2002Co-Authors: Nagahito Saito, Fujio Sato, Mototsugu Kato, Hiroshi Takeda, Toshiro Sugiyama, Masahiro AsakaAbstract:Background.Helicobacter pylori adhering to the human gastric epithelium causes gastric diseases such as ulcer, carcinoma and lymphoma. It is thus important to observe in detail both the surface of the epithelial cells and the H. pylori that adhered to it for the elucidation of H. pylori-induced diseases by scanning electron microscopy (SEM). Since the thick mucus layer blocks the observation of the cell surface and the bacteria, it is generally eliminated during the processing for SEM by roughly mechanical methods, but these treatments also demolish the ultrastructure of the cells. We studied the nonmechanical method for removal of mucus layer of gastric epithelium using Pronase. Materials and Methods. To determine the optimal concentration of Pronase, mucin was used as a substrate for inhibition of the viscosity. Pronase was added in 2% mucin at the concentration of 10, 50, 100, 500, 1000, 2000 or 5000 unit/ml and the flowing time of the mixture was measured. Based on the digestion experiment, biopsied specimens from 24 patients with dyspepsic symptoms were fixed in glutaraldehyde and then washed in rolling with different concentration of Pronase. After the pretreatment by Pronase, the specimens were treated according to the standard process for SEM. Results. We succeeded in removing the mucus layer on the surface of epithelial cells from the biopsied specimens fixed in glutaraldehyde by rinsing with 2000 unit/ml Pronase for 24 hours. Conclusions. Using our digestive method without destroying the ultrastructure, the earliest stage which H. pylori has adhered onto the human gastric epithelium can be observed for the investigation of H. pylori-induced gastric disorders by SEM.
J A Ordonez - One of the best experts on this subject based on the ideXlab platform.
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combined use of Pronase e and a fungal extract penicillium aurantiogriseum to potentiate the sensory characteristics of dry fermented sausages
Meat Science, 2000Co-Authors: Jose M Bruna, Manuela Fernandez, Eva Hierro, J A OrdonezAbstract:Abstract A combination of an extract of Penicillium aurantiogriseum and Pronase E was used in an attempt to enhance the sensory characteristics of dry fermented sausages, specifically, a salami-like one named “salchichon”. The addition of Pronase E alone (600 units/kg) produced a rise in free amino acids and biogenic amines and also an increase in the ammonia content. Addition of the protease and fungal extract (100.87 mg protein/kg of mixture) brought about a decrease in the level of free amino acids and a larger increase in the ammonia content than the batch added with only Pronase E. There was also an increase in the amount of specific volatile compounds such as 2- and 3-methylbutanal, 2- and 3-methylpropanal and 2- and 3-methyl-1-butanol produced by the breakdown of amino acids in these fermented sausages. Values of pH, a w and dry matter were not affected by addition of the protease or fungal extract. In contrast, addition of Pronase E produced a notable change in the textural characteristics, reducing the hardness, cohesiveness, gumminess, chewiness and cutting force. Sensory analysis showed that fermented sausages manufactured with Pronase E and Penicillium aurantiogriseum extract had better odour, flavour, texture and, as a consequence, better general acceptability.
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effect of the addition of Pronase e on the proteolysis in dry fermented sausages
Meat Science, 1993Co-Authors: Olga Heredero Diaz, Manuela Fernandez, Gonzalo Garcia D De Fernando, J A OrdonezAbstract:Abstract The effect of the addition of Pronase E at two different concentrations on protein breakdown during the ripening of dry fermented sausage was studied. In all batches, water-soluble, non-protein and 5% phosphotungstic acid soluble nitrogens increased sharply during the first days of ripening, then became stabilized until the end of the process (26th day), and the total volatile nitrogen consistently increased during ripening. The greater the Pronase E added the higher were the values reached for all these fractions. The changes in total free amino acids showed a similar pattern to that observed for the phosphotungstic acid soluble nitrogen. Histamine and tyramine progressively increased throughout the ripening. By sensory evaluation, no significant differences between the control batch and the batch with the lowest amount of added Pronase E were found, but both batches were significantly different (P
Ilkwun Chung - One of the best experts on this subject based on the ideXlab platform.
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efforts to increase image quality during endoscopy the role of Pronase
World Journal of Gastrointestinal Endoscopy, 2016Co-Authors: Ilkwun ChungAbstract:Core tip: The present review discusses the role of Pronase in increasing image quality during endoscopy. Premedication with Pronase 10 to 20 min before endoscopy significantly improves mucosal visibility without affecting the accuracy of Helicobacter pylori identification. The effects of Pronase as premedication are also applicable in advanced endoscopic procedures such as narrow-band imaging, magnifying endoscopy, or endoscopic ultrasonography. Although improved mucosal visibility does not necessarily improve clinical outcomes, premedication with Pronase may be helpful for increasing the detection rate of early cancers.
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effect of Pronase as mucolytic agent on imaging quality of magnifying endoscopy
World Journal of Gastroenterology, 2015Co-Authors: Ilkwun ChungAbstract:AIM: To investigate the efficacy of premedication with Pronase, a proteolytic enzyme, in improving image quality during magnifying endoscopy. METHODS: The study was of a blinded, randomized, prospective design. Patients were assigned to groups administered oral premedication of either Pronase and simethicone (Group A) or simethicone alone (Group B). First, the gastric mucosal visibility grade (1-4) was determined during conventional endoscopy, and then a magnifying endoscopic examination was conducted. The quality of images obtained by magnifying endoscopy at the stomach and the esophagus was scored from 1 to 3, with a lower score indicating better visibility. The endoscopist used water flushes as needed to obtain satisfactory magnifying endoscopic views. The main study outcomes were the visibility scores during magnifying endoscopy and the number of water flushes. RESULTS: A total of 144 patients were enrolled, and data from 143 patients (M:F = 90:53, mean age 57.5 years) were analyzed. The visibility score was significantly higher in the stomach following premedication with Pronase (73% with a score of 1 in Group A vs 49% in Group B, P < 0.05), but there was no difference in the esophagus visibility scores (67% with a score of 1 in Group A vs 58% in Group B). Fewer water flushes [mean 0.7 ± 0.9 times (range: 0-3 times) in Group A vs 1.9 ± 1.5 times (range: 0-6 times) in Group B, P < 0.05] in the Pronase premedication group did not affect the endoscopic procedure times [mean 766 s (range: 647-866 s) for Group A vs 760 s (range: 678-854 s) for Group B, P = 0.88]. The total gastric mucosal visibility score was also lower in Group A (4.9 ± 1.5 vs 8.3 ± 1.8 in Group B, P < 0.01). CONCLUSION: The addition of Pronase to simethicone premedication resulted in clearer images during magnifying endoscopy and reduced the need for water flushes.
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endoscopic flushing with Pronase improves the quantity and quality of gastric biopsy a prospective study
Endoscopy, 2014Co-Authors: Ilkwun ChungAbstract:Background and study aims: Pronase, a proteolytic enzyme, is known to improve mucosal visibility during esophagogastroduodenoscopy (EGD), but little is known about its effects on gastric biopsy. This study assessed whether endoscopic flushing with Pronase improves the quality of gastric biopsy. Patients and methods: Consecutive patients who underwent EGD were randomly assigned to either the control group or the Pronase group in a prospective setting. The first biopsy of the identified lesion was performed during endoscopy. Endoscopic flushing with either 50 mL of water and dimethylpolysiloxane (DMPS; control group) or 50 mL of water, Pronase, sodium bicarbonate, and DMPS (Pronase group) was then applied to the lesion. After 5 minutes, the second biopsy was performed 2 – 3 mm away from the first biopsy site. The thickness of mucus, depth of the specimen, overall diagnostic adequacy, anatomical orientation, and crush artifact were measured to assess the quality of the biopsy. Results: Of the 208 patients, 10 were not analyzed due to the absence of an identifiable lesion. Compared with the control group, the Pronase group showed significantly decreased thickness of mucus (P Conclusions: Endoscopic flushing with Pronase not only improved the depth of biopsy but also the anatomical orientation and overall diagnostic adequacy. Pronase can be recommended for flushing during EGD to improve the quantity and quality of biopsy.
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Endoscopic flushing with Pronase improves the quantity and quality of gastric biopsy: a prospective study.
Endoscopy, 2014Co-Authors: Ilkwun ChungAbstract:Pronase, a proteolytic enzyme, is known to improve mucosal visibility during esophagogastroduodenoscopy (EGD), but little is known about its effects on gastric biopsy. This study assessed whether endoscopic flushing with Pronase improves the quality of gastric biopsy. Consecutive patients who underwent EGD were randomly assigned to either the control group or the Pronase group in a prospective setting. The first biopsy of the identified lesion was performed during endoscopy. Endoscopic flushing with either 50 mL of water and dimethylpolysiloxane (DMPS; control group) or 50 mL of water, Pronase, sodium bicarbonate, and DMPS (Pronase group) was then applied to the lesion. After 5 minutes, the second biopsy was performed 2 - 3 mm away from the first biopsy site. The thickness of mucus, depth of the specimen, overall diagnostic adequacy, anatomical orientation, and crush artifact were measured to assess the quality of the biopsy. Of the 208 patients, 10 were not analyzed due to the absence of an identifiable lesion. Compared with the control group, the Pronase group showed significantly decreased thickness of mucus (P < 0.001), increased depth of biopsy (P < 0.001), improved anatomical orientation (P = 0.010), and improved overall diagnostic assessment (P = 0.011) in the second biopsied specimen following endoscopic flushing. The crush artifact and hemorrhage did not differ between the groups. Endoscopic flushing with Pronase not only improved the depth of biopsy but also the anatomical orientation and overall diagnostic adequacy. Pronase can be recommended for flushing during EGD to improve the quantity and quality of biopsy. © Georg Thieme Verlag KG Stuttgart · New York.
Won Moon - One of the best experts on this subject based on the ideXlab platform.
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effectiveness of premedication with Pronase for visualization of the mucosa during endoscopy a randomized controlled trial
Clinical Endoscopy, 2012Co-Authors: Seun Ja Park, Moo In Park, Won MoonAbstract:BACKGROUND/AIMS: Gastric mucus should be removed before endoscopic examination to increase visibility. In this study, the effectiveness of premedication with Pronase for improving visibility during endoscopy was investigated. METHODS: From April 2010 to February 2011, 400 outpatients were randomly assigned to receive endoscopy with one of four premedications as follows: dimethylpolysiloxane (DMPS), Pronase and sodium bicarbonate with 10 minutes premedication time (group A, n=100), DMPS and sodium bicarbonate with 10 minutes premedication time (group B, n=100), DMPS, Pronase and sodium bicarbonate with 20 minutes premedication time (group C, n=100), and DMPS and sodium bicarbonate with 20 minute premedication time (group D, n=100). One endoscopist, who was unaware of the premedication types, calculated the visibility scores (range, 1 to 3) of the antrum, lower gastric body, upper gastric body and fundus. The sum of the scores from the four locations was defined as the total visibility score. RESULTS: Group C showed significantly lower scores than other groups (p=0.002). Group C also had the lowest frequency of flushing, which was significantly lower than that of group D. Groups C and D had significantly shorter durations of examination than groups A and B. CONCLUSIONS: Using Pronase 20 minutes before endoscopy significantly improved endoscopic visualization and decreased the frequency of water flushing.
