The Experts below are selected from a list of 6006 Experts worldwide ranked by ideXlab platform
Tao Yang - One of the best experts on this subject based on the ideXlab platform.
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Dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
Neural regeneration research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia reperfusion and oxygen glucose deprivation
Neural Regeneration Research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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Defective neuropeptide processing and ischemic brain injury: a study on Proprotein Convertase 2 and its substrate neuropeptide in ischemic brains
Journal of Cerebral Blood Flow & Metabolism, 2009Co-Authors: Shuqin Zhan, Tao Yang, Hongbo Zhao, Aaron J White, Manabu Minami, Giuseppe Pignataro, Xiaorong Zhu, Jing-quan Lan, Zhi-gang Xiong, Donald F. SteinerAbstract:Using a focal cerebral ischemia model in rats, brain ischemia-induced changes in expression levels of mRNA and protein, and activities of Proprotein Convertase 2 (PC2) in the cortex were examined. In situ hybridization analyses revealed a transient upregulation of the mRNA level for PC2 at an early reperfusion hour, at which the level of PC2 protein was also high as determined by immunocytochemistry and western blotting. When enzymatic activities of PC2 were analyzed using a synthetic substrate, a significant decrease was observed at early reperfusion hours at which levels of PC2 protein were still high. Also decreased at these reperfusion hours were tissue levels of dynorphin-A(1-8) (DYN-A(1-8)), a PC2 substrate, as determined by radioimmunoassay. Further examination of PC2 protein biosynthesis by metabolic labeling in cultured neuronal cells showed that in ischemic cells, the proteolytic processing of PC2 was greatly attenuated. Finally, in mice, an intracerebroventricular administration of synthetic DYN-A(1-8) significantly reduced the extent of ischemic brain injury. In mice those lack an active PC2, exacerbated brain injury was observed after an otherwise non-lethal focal ischemia. We conclude that brain ischemia attenuates PC2 and PC2-mediated neuropeptide processing. This attenuation may play a role in the pathology of ischemic brain injury.
Shuqin Zhan - One of the best experts on this subject based on the ideXlab platform.
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Dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
Neural regeneration research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia reperfusion and oxygen glucose deprivation
Neural Regeneration Research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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Defective neuropeptide processing and ischemic brain injury: a study on Proprotein Convertase 2 and its substrate neuropeptide in ischemic brains
Journal of Cerebral Blood Flow & Metabolism, 2009Co-Authors: Shuqin Zhan, Tao Yang, Hongbo Zhao, Aaron J White, Manabu Minami, Giuseppe Pignataro, Xiaorong Zhu, Jing-quan Lan, Zhi-gang Xiong, Donald F. SteinerAbstract:Using a focal cerebral ischemia model in rats, brain ischemia-induced changes in expression levels of mRNA and protein, and activities of Proprotein Convertase 2 (PC2) in the cortex were examined. In situ hybridization analyses revealed a transient upregulation of the mRNA level for PC2 at an early reperfusion hour, at which the level of PC2 protein was also high as determined by immunocytochemistry and western blotting. When enzymatic activities of PC2 were analyzed using a synthetic substrate, a significant decrease was observed at early reperfusion hours at which levels of PC2 protein were still high. Also decreased at these reperfusion hours were tissue levels of dynorphin-A(1-8) (DYN-A(1-8)), a PC2 substrate, as determined by radioimmunoassay. Further examination of PC2 protein biosynthesis by metabolic labeling in cultured neuronal cells showed that in ischemic cells, the proteolytic processing of PC2 was greatly attenuated. Finally, in mice, an intracerebroventricular administration of synthetic DYN-A(1-8) significantly reduced the extent of ischemic brain injury. In mice those lack an active PC2, exacerbated brain injury was observed after an otherwise non-lethal focal ischemia. We conclude that brain ischemia attenuates PC2 and PC2-mediated neuropeptide processing. This attenuation may play a role in the pathology of ischemic brain injury.
Howard W. Davidson - One of the best experts on this subject based on the ideXlab platform.
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(Pro)Insulin processing
Cell Biochemistry and Biophysics, 2004Co-Authors: Howard W. DavidsonAbstract:Insulin, the major secreted product of the β-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identification of the central processing enzymes as Proprotein Convertase 1, Proprotein Convertase 2, and carboxypeptidase E. It also discusses the central roles of the intragranular environment and chaperone-like proteins in modulating processing activity.
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(Pro)Insulin processing: a historical perspective.
Cell Biochemistry and Biophysics, 2004Co-Authors: Howard W. DavidsonAbstract:Insulin, the major secreted product of the β-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identification of the central processing enzymes as Proprotein Convertase 1, Proprotein Convertase 2, and carboxypeptidase E. It also discusses the central roles of the intragranular environment and chaperone-like proteins in modulating processing activity.
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(Pro)Insulin processing: a historical perspective.
Cell biochemistry and biophysics, 2004Co-Authors: Howard W. DavidsonAbstract:Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identification of the central processing enzymes as Proprotein Convertase 1, Proprotein Convertase 2, and carboxypeptidase E. It also discusses the central roles of the intragranular environment and chaperone-like proteins in modulating processing activity.
Chelsea Piper - One of the best experts on this subject based on the ideXlab platform.
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Dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
Neural regeneration research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia reperfusion and oxygen glucose deprivation
Neural Regeneration Research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
An Zhou - One of the best experts on this subject based on the ideXlab platform.
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Dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
Neural regeneration research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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dynamic changes in Proprotein Convertase 2 activity in cortical neurons after ischemia reperfusion and oxygen glucose deprivation
Neural Regeneration Research, 2013Co-Authors: Shuqin Zhan, An Zhou, Chelsea Piper, Tao YangAbstract:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein Convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and Proprotein Convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that Proprotein Convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
