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José M. Franco - One of the best experts on this subject based on the ideXlab platform.
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morphology molecular phylogeny and toxinology of coolia and Prorocentrum strains isolated from the tropical south western atlantic ocean
Botanica Marina, 2019Co-Authors: Maria Cristina De Queiroz Mendes, Pilar Riobó, José M. Franco, Francisco Rodriguez, Suema Branco, Jose Marcos De Castro Nunes, Santiago Fraga, Mariângela MenezesAbstract:The morphology, molecular phylogeny and toxinology of two Coolia and one Prorocentrum dinoflagellate strains from Brazil were characterized. They matched with Coolia malayensis and Coolia tropicalis morphotypes, while the Prorocentrum strain fitted well with the morphology of Prorocentrum emarginatum. Complementary identification by molecular analyses was carried out based on LSU and ITS-5.8S rDNA. Phylogenetic analyses of Coolia strains (D1/D2 region, LSU rDNA), showed that C. malayensis (strain UFBA044) segregated together with sequences of this species from other parts of the world, but diverged earlier in a separate branch to sequences from Sao Paulo (Brazil) or Caribbean areas. Coolia tropicalis (strain UFBA055) grouped with other sequences of this species, in a subclade with an isolate from Belize, closer to a subgroup including isolates from Thailand, Australia and Hong Kong. Phylogenetic analyses (ITS-5.8S rDNA) of P. emarginatum (strain UFBA033) grouped together with another sequence of this species from China Sea. Diarrhetic shellfish poisoning toxins (OA, DTXs and PTX2) were not detected in P. emarginatum by mass spectrometry analyses. However, hemolytic assays in P. emarginatum and both Coolia strains in this study showed positive results.
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preliminary study for rapid determination of phycotoxins in microalgae whole cells using matrix assisted laser desorption ionization time of flight mass spectrometry
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Beatriz Paz, Pilar Riobó, José M. FrancoAbstract:Rapid and sensitive methods for identification of several phycotoxins produced by microalgae species such as yessotoxins (YTXs) for Protoceratium reticulatum, okadaic acid (OA) and pectenotoxins (PTXs) for Prorocentrum spp. and Dinophysis spp., Palytoxins (PLTXs) for Ostreopsis spp., ciguatoxins (CTXs) for Gambierdiscus spp. or domoic acid (DA) for Pseudo-nitzschia spp. are of great importance to the shellfish and fish industry. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to detect several phycotoxins in whole cells of some microalgae which are known as toxin producers. To achieve an appropriate MALDI matrix and a sample preparation method, several matrices and solvent mixtures were tested. The most appropriate matrix system for toxin detection was obtained with 10 µg μL–1 of DHB in 0.1% TFA/ACN (3:7, v/v) by mixing the intact cells with the matrix solution directly on the MALDI target (dried-droplet technique). Toxin detection by this procedure is much faster than current procedures based on solvent extraction and chromatographic separation. This method allowed the rapid detection of main phycotoxins in some dinoflagellate cells of genus Ostreopsis, Prorocentrum, Protoceratium, Gambierdiscus, Dinophysis and diatoms from Pseudo-nitzschia genus. Copyright © 2011 John Wiley & Sons, Ltd.
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identification of 19 epi okadaic acid a new diarrhetic shellfish poisoning toxin by liquid chromatography with mass spectrometry detection
Marine Drugs, 2008Co-Authors: Beatriz Paz, José M. Franco, Manuel Norte, Antonio Hernandez Daranas, Patricia G Cruz, Jose J FernandezAbstract:Okadaic acid (1) (OA) and its congeners are mainly responsible for diarrhetic shellfish poisoning (DSP) syndrome. The presence of several OA derivatives have already been confirmed in Prorocentrum and Dinophysis spp. In this paper, we report on the detection and identification of a new DSP toxin, the OA isomer 19-epi-okadaic acid (2) (19-epi-OA), isolated from cultures of Prorocentrum belizeanum, by determining its retention time (RT) and fragmentation pattern using liquid chromatography coupled with mass spectrometry (LC–MS/MS).
Pilar Riobó - One of the best experts on this subject based on the ideXlab platform.
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morphology genetics and toxin profile of Prorocentrum texanum dinophyceae from argentinian marine coastal waters
Phycologia, 2020Co-Authors: Ines Sunesen, Pilar Riobó, Francisco Rodriguez Hernandez, Delfina Aguiar Juarez, Jonas Adrian Tardivo Kubis, Andrea S Lavigne, Araceli E Rossignoli, Eugenia A SarAbstract:Two strains of Prorocentrum texanum (LPCc020 and LPCc032) were established from coastal waters of the Province of Buenos Aires, Argentina. Field and cultured materials were examined using light and...
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morphology molecular phylogeny and toxinology of coolia and Prorocentrum strains isolated from the tropical south western atlantic ocean
Botanica Marina, 2019Co-Authors: Maria Cristina De Queiroz Mendes, Pilar Riobó, José M. Franco, Francisco Rodriguez, Suema Branco, Jose Marcos De Castro Nunes, Santiago Fraga, Mariângela MenezesAbstract:The morphology, molecular phylogeny and toxinology of two Coolia and one Prorocentrum dinoflagellate strains from Brazil were characterized. They matched with Coolia malayensis and Coolia tropicalis morphotypes, while the Prorocentrum strain fitted well with the morphology of Prorocentrum emarginatum. Complementary identification by molecular analyses was carried out based on LSU and ITS-5.8S rDNA. Phylogenetic analyses of Coolia strains (D1/D2 region, LSU rDNA), showed that C. malayensis (strain UFBA044) segregated together with sequences of this species from other parts of the world, but diverged earlier in a separate branch to sequences from Sao Paulo (Brazil) or Caribbean areas. Coolia tropicalis (strain UFBA055) grouped with other sequences of this species, in a subclade with an isolate from Belize, closer to a subgroup including isolates from Thailand, Australia and Hong Kong. Phylogenetic analyses (ITS-5.8S rDNA) of P. emarginatum (strain UFBA033) grouped together with another sequence of this species from China Sea. Diarrhetic shellfish poisoning toxins (OA, DTXs and PTX2) were not detected in P. emarginatum by mass spectrometry analyses. However, hemolytic assays in P. emarginatum and both Coolia strains in this study showed positive results.
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the toxic benthic dinoflagellate Prorocentrum maculosum faust is a synonym of Prorocentrum hoffmannianum faust
Harmful Algae, 2018Co-Authors: Francisco Rodriguez, Pilar Riobó, Manuel Norte, Jose J Fernandez, Antonio Hernandez Daranas, Caterina R. De Vera, Guillermo Díaz Crespín, Santiago FragaAbstract:Abstract Three strains of the toxic benthic dinoflagellate Prorocentrum hoffmannianum were isolated in the Canary Islands (north-east Atlantic Ocean, Spain). The identity of the strains was determined by phylogenetic analyses of partial LSU rDNA (D1-D2 regions) but their morphology based on SEM images corresponded to P. maculosum. Their toxin profiles were analyzed by liquid chromatography and high resolution mass spectrometry analysis (LC–HRMS) on cell extracts and culture media. Okadaic acid and three analogs were detected in all strains. Rather, in culture media the detected compounds were variable among strains, two of them being okadaic acid analogs not found on cell extracts. As a result, the taxonomy of the species was revised and P. maculosum is proposed as a junior synonym of P. hoffmannianum whose description is emended.
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morphology and phylogeny of Prorocentrum caipirignum sp nov dinophyceae a new tropical toxic benthic dinoflagellate
Harmful Algae, 2017Co-Authors: Silvia M Nascimento, Pilar Riobó, Cristina M Q Mendes, Mariângela Menezes, Francisco Rodriguez, Catharina Alvesdesouza, Suema Branco, J M Franco, Jose Marcos De Castro Nunes, Mariusz HukAbstract:A new species of toxic benthic dinoflagellate is described based on laboratory cultures isolated from two locations from Brazil, Rio de Janeiro and Bahia. The morphology was studied with SEM and LM. Cells are elliptical in right thecal view and flat. They are 37-44μm long and 29-36μm wide. The right thecal plate has a V shaped indentation where six platelets can be identified. The thecal surface of both thecal plates is smooth and has round or kidney shaped and uniformly distributed pores except in the central area of the cell, and a line of marginal pores. Some cells present an elongated depression on the central area of the apical part of the right thecal plate. Prorocentrum caipirignum is similar to Prorocentrum lima in its morphology, but can be differentiated by the general cell shape, being elliptical while P. lima is ovoid. In the phylogenetic trees based on ITS and LSU rDNA sequences, the P. caipirignum clade appears close to the clades of P. lima and Prorocentrum hoffmannianum. The Brazilian strains of P. caipirignum formed a clade with strains from Cuba, Hainan Island and Malaysia and it is therefore likely that this new species has a broad tropical distribution. Prorocentrum caipirignum is a toxic species that produces okadaic acid and the fast acting toxin prorocentrolide.
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preliminary study for rapid determination of phycotoxins in microalgae whole cells using matrix assisted laser desorption ionization time of flight mass spectrometry
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Beatriz Paz, Pilar Riobó, José M. FrancoAbstract:Rapid and sensitive methods for identification of several phycotoxins produced by microalgae species such as yessotoxins (YTXs) for Protoceratium reticulatum, okadaic acid (OA) and pectenotoxins (PTXs) for Prorocentrum spp. and Dinophysis spp., Palytoxins (PLTXs) for Ostreopsis spp., ciguatoxins (CTXs) for Gambierdiscus spp. or domoic acid (DA) for Pseudo-nitzschia spp. are of great importance to the shellfish and fish industry. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to detect several phycotoxins in whole cells of some microalgae which are known as toxin producers. To achieve an appropriate MALDI matrix and a sample preparation method, several matrices and solvent mixtures were tested. The most appropriate matrix system for toxin detection was obtained with 10 µg μL–1 of DHB in 0.1% TFA/ACN (3:7, v/v) by mixing the intact cells with the matrix solution directly on the MALDI target (dried-droplet technique). Toxin detection by this procedure is much faster than current procedures based on solvent extraction and chromatographic separation. This method allowed the rapid detection of main phycotoxins in some dinoflagellate cells of genus Ostreopsis, Prorocentrum, Protoceratium, Gambierdiscus, Dinophysis and diatoms from Pseudo-nitzschia genus. Copyright © 2011 John Wiley & Sons, Ltd.
Beatriz Paz - One of the best experts on this subject based on the ideXlab platform.
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preliminary study for rapid determination of phycotoxins in microalgae whole cells using matrix assisted laser desorption ionization time of flight mass spectrometry
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Beatriz Paz, Pilar Riobó, José M. FrancoAbstract:Rapid and sensitive methods for identification of several phycotoxins produced by microalgae species such as yessotoxins (YTXs) for Protoceratium reticulatum, okadaic acid (OA) and pectenotoxins (PTXs) for Prorocentrum spp. and Dinophysis spp., Palytoxins (PLTXs) for Ostreopsis spp., ciguatoxins (CTXs) for Gambierdiscus spp. or domoic acid (DA) for Pseudo-nitzschia spp. are of great importance to the shellfish and fish industry. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to detect several phycotoxins in whole cells of some microalgae which are known as toxin producers. To achieve an appropriate MALDI matrix and a sample preparation method, several matrices and solvent mixtures were tested. The most appropriate matrix system for toxin detection was obtained with 10 µg μL–1 of DHB in 0.1% TFA/ACN (3:7, v/v) by mixing the intact cells with the matrix solution directly on the MALDI target (dried-droplet technique). Toxin detection by this procedure is much faster than current procedures based on solvent extraction and chromatographic separation. This method allowed the rapid detection of main phycotoxins in some dinoflagellate cells of genus Ostreopsis, Prorocentrum, Protoceratium, Gambierdiscus, Dinophysis and diatoms from Pseudo-nitzschia genus. Copyright © 2011 John Wiley & Sons, Ltd.
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identification of 19 epi okadaic acid a new diarrhetic shellfish poisoning toxin by liquid chromatography with mass spectrometry detection
Marine Drugs, 2008Co-Authors: Beatriz Paz, José M. Franco, Manuel Norte, Antonio Hernandez Daranas, Patricia G Cruz, Jose J FernandezAbstract:Okadaic acid (1) (OA) and its congeners are mainly responsible for diarrhetic shellfish poisoning (DSP) syndrome. The presence of several OA derivatives have already been confirmed in Prorocentrum and Dinophysis spp. In this paper, we report on the detection and identification of a new DSP toxin, the OA isomer 19-epi-okadaic acid (2) (19-epi-OA), isolated from cultures of Prorocentrum belizeanum, by determining its retention time (RT) and fragmentation pattern using liquid chromatography coupled with mass spectrometry (LC–MS/MS).
Wardhana Wisnu - One of the best experts on this subject based on the ideXlab platform.
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SEBARAN SPASIAL SPESIES PENYEBAB HARMFUL ALGAL BLOOM (HAB) DI LOKASI BUDIDAYA KERANG HIJAU (Perna viridis) KAMAL MUARA, JAKARTA UTARA, PADA BULAN MEI 2011
'Fakultas Perikanan dan Ilmu Kelautan Universitas Papua', 2012Co-Authors: - Mulyani, Widiarti Riani, Wardhana WisnuAbstract:Telah dilakukan penelitian tentang sebaran spasial spesies HAB di lokasi budidaya kerang hijau (Perna viridis), Kamal Muara, Jakarta Utara pada bulan Mei 2011. Sampel diambil secara vertikal di sembilan stasiun dengan plankton-net. Spesies HAB yang ditemukan berasal dari kelas Dinophyceae (Ceratium furca, Dinophysis caudata, Gonyaulax polygramma, Gonyaulax spinifera, Gymnodinium catenatum, Gymnodinium sanguineum, Prorocentrum micans, dan Prorocentrum sigmoides); Bacillariophyceae (Nitzschia spp., Chaetoceros spp., Skeletonema costatum, dan Thalassiosira spp.) dan Raphidophyceae (Chattonella spp.). Berdasarkan peta isoplank diketahui bahwa sebaran terpadat pada bulan Mei 2011 adalah di stasiun dekat muara. Kata kunci : Harmful Algal Blooms, Kamal Muara, kerang hijau, dan sebaran spasia
Wardhana W. - One of the best experts on this subject based on the ideXlab platform.
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Sebaran Spasial Spesies Penyebab Harmful Algal Bloom (Hab) di Lokasi Budidaya Kerang Hijau (Perna Viridis) Kamal Muara, Jakarta Utara, pada Bulan Mei 2011
Padjadjaran University, 2012Co-Authors: Mulyani M., Widiarti R., Wardhana W.Abstract:Telah dilakukan penelitian tentang sebaran spasial spesies HAB di lokasi budidaya kerang hijau (Perna viridis), Kamal Muara, Jakarta Utara pada bulan Mei 2011. Sampel diambil secara vertikal di sembilan stasiun dengan plankton-net. Spesies HAB yang ditemukan berasal dari kelas Dinophyceae (Ceratium furca, Dinophysis caudata, Gonyaulax polygramma, Gonyaulax spinifera, Gymnodinium catenatum, Gymnodinium sanguineum, Prorocentrum micans, dan Prorocentrum sigmoides); Bacillariophyceae (Nitzschia spp., Chaetoceros spp., Skeletonema costatum, dan Thalassiosira spp.) dan Raphidophyceae (Chattonella spp.). Berdasarkan peta isoplank diketahui bahwa sebaran terpadat pada bulan Mei 2011 adalah di stasiun dekat muara
