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G A Patterson - One of the best experts on this subject based on the ideXlab platform.
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equivalent eighteen hour lung preservation with low potassium dextran or euro collins solution after Prostaglandin E1 infusion
The Journal of Thoracic and Cardiovascular Surgery, 1992Co-Authors: John D Puskas, Paulo Francisco Guerreiro Cardoso, A S Slutsky, E Mayer, Shiquig Shi, G A PattersonAbstract:Improved techniques of pulmonary preservation would help alleviate the critical shortage of donor organs in lung transplantation and would improve early graft function. A previous study demonstrated that cold pulmonary artery flush with low-potassium dextran solution was superior to Euro-Collins solution in preservation of canine lung allografts stored for 12 hours when no pulmonary vasodilator was used before donor lung flush. The present study was designed to determine whether donor pretreatment with Prostaglandin E1 would affect the superiority of low-potassium dextran as a preservation solution. Prostaglandin E1 was infused (50 μg/min) in 12 donor dogs until potent vasodilatation was demonstrated. Low-pressure pulmonary artery flush (50 ml/kg) with either Euro-Collins or low-potassium dextran solution (n = 6 for each group) was performed at 4° C in a randomized, blinded fashion. Heart-lung blocks were extracted and stored at 4° C for 18 hours before left lung allografting. Inflatable cuffs were placed around each pulmonary artery, allowing independent study of the native and transplanted lungs. All 12 recipient dogs survived the 3-day assessment period. Lungs flushed and stored in Euro-Collins or low-potassium dextran solution provided equivalent gas exchange function on day 0 (arterial oxygen tension: Euro-Collins 289 ±105 mm Hg versus low-potassium dextran 265 ± 111 mm Hg; mean ± standard error of the mean) and on day 3 (Euro-Collins 516 ± 45 mm Hg versus low-potassium dextran 354 ± 77 mm Hg; p = 0.10). Mean pulmonary artery pressures in the transplanted lung were not significantly different in the Euro-Collins and low-potassium dextran groups on day 0 (21.4 ± 2 mm Hg versus 33.7 ± 5 mm Hg, respectively; p = 0.09) or on day 3 (20.2 ± 2.7 mm Hg versus 24.2 ± 5.1 mm Hg, respectively; p = 0.50). We conclude that there was no advantage of low-potassium dextran over Euro-Collins as a flush solution in this 18-hour canine single lung allograft model in which Prostaglandin E1 was administered before pulmonary artery flush. (J T horac C ardiovasc S urg 1992;104:83–9)
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equivalent eighteen hour lung preservation with low potassium dextran or euro collins solution after Prostaglandin E1 infusion
The Journal of Thoracic and Cardiovascular Surgery, 1992Co-Authors: John D Puskas, Paulo Francisco Guerreiro Cardoso, A S Slutsky, E Mayer, Shiquig Shi, G A PattersonAbstract:Improved techniques of pulmonary preservation would help alleviate the critical shortage of donor organs in lung transplantation and would improve early graft function. A previous study demonstrated that cold pulmonary artery flush with low-potassium dextran solution was superior to Euro-Collins solution in preservation of canine lung allografts stored for 12 hours when no pulmonary vasodilator was used before donor lung flush. The present study was designed to determine whether donor pretreatment with Prostaglandin E1 would affect the superiority of low-potassium dextran as a preservation solution. Prostaglandin E1 was infused (50 micrograms/min) in 12 donor dogs until potent vasodilation was demonstrated. Low-pressure pulmonary artery flush (50 ml/kg) with either Euro-Collins or low-potassium dextran solution (n = 6 for each group) was performed at 4 degrees C in a randomized, blinded fashion. Heart-lung blocks were extracted and stored at 4 degrees C for 18 hours before left lung allografting. Inflatable cuffs were placed around each pulmonary artery, allowing independent study of the native and transplanted lungs. All 12 recipient dogs survived the 3-day assessment period. Lungs flushed and stored in Euro-Collins or low-potassium dextran solution provided equivalent gas exchange function on day 0 (arterial oxygen tension: Euro-Collins 289 +/- 105 mm Hg versus low-potassium dextran 265 +/- 111 mm Hg; mean +/- standard error of the mean) and on day 3 (Euro-Collins 516 +/- 45 mm Hg versus low-potassium dextran 354 +/- 77 mm Hg; p = 0.10). Mean pulmonary artery pressures in the transplanted lung were not significantly different in the Euro-Collins and low-potassium dextran groups on day 0 (21.4 +/- 2 mm Hg versus 33.7 +/- 5 mm Hg, respectively; p = 0.09) or on day 3 (20.2 +/- 2.7 mm Hg versus 24.2 +/- 5.1 mm Hg, respectively; p = 0.50). We conclude that there was no advantage of low-potassium dextran over Euro-Collins as a flush solution in this 18-hour canine single lung allograft model in which Prostaglandin E1 was administered before pulmonary artery flush.
Wayne J G Hellstrom - One of the best experts on this subject based on the ideXlab platform.
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standardization of penile blood flow parameters in normal men using intracavernous Prostaglandin E1 and visual sexual stimulation
The Journal of Urology, 1993Co-Authors: Suresh C Sikka, Peggy Villemarette, John F Hower, Eduardo Randrup, Neil Baum, Wayne J G HellstromAbstract:AbstractThe evaluation of vasculogenic impotence by color flow Doppler ultrasound after injection of intracavernous vasoactive agents allows for simultaneous visualization in real time of arterial and venous blood flow. Normal arterial blood flow parameters after Prostaglandin E1 injection have yet to be standardized. Our study was initiated to evaluate blood flow parameters in a normal control population after Prostaglandin E1 and visual stimulation.A total of 20 healthy male volunteers 45 to 60 years old with histories of normal sexual function was selected. All volunteers were given intracavernous injections of 10 μg. Prostaglandin E1 and received concurrent visual stimulation by means of an erotic video. All patients developed rigid erections with no complications. Using color flow Doppler ultrasound measurements were done before and after Prostaglandin E1 injection of right and left superficial and deep cavernous artery diameters, peak blood flow velocities and blood flow volumes.Results (mean plus o...
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standardization of penile blood flow parameters in normal men using intracavernous Prostaglandin E1 and visual sexual stimulation
The Journal of Urology, 1993Co-Authors: Barry Lee, Peggy Villemarette, John F Hower, Eduardo Randrup, Suresh C Sikka, Neil Baum, Wayne J G HellstromAbstract:The evaluation of vasculogenic impotence by color flow Doppler ultrasound after injection of intracavernous vasoactive agents allows for simultaneous visualization in real time of arterial and venous blood flow. Normal arterial blood flow parameters after Prostaglandin E1 injection have yet to be standardized. Our study was initiated to evaluate blood flow parameters in a normal control population after Prostaglandin E1 and visual stimulation. A total of 20 healthy male volunteers 45 to 60 years old with histories of normal sexual function was selected. All volunteers were given intracavernous injections of 10 micrograms Prostaglandin E1 and received concurrent visual stimulation by means of an erotic video. All patients developed rigid erections with no complications. Using color flow Doppler ultrasound measurements were done before and after Prostaglandin E1 injection of right and left superficial and deep cavernous artery diameters, peak blood flow velocities and blood flow volumes. Results (mean plus or minus standard error) showed a significant increase in diameters after Prostaglandin E1 in the superficial (20% increase) and deep (70% increase) penile arteries. Blood flow volume increased 3-fold for the superficial penile arteries (from 7.3 +/- 1.4 to 20 +/- 3.5 cc per minute) and 4-fold for the deep cavernous arteries (from 3.8 +/- 1 to 12.5 +/- 1.8 cc per minute). Peak blood flow velocity increased 2-fold (from 22 +/- 3 to 46 +/- 7 cm. per second) for the superficial arteries and 3-fold (from 12.5 +/- 2 to 37 +/- 5 cm. per second) for the deep cavernous arteries. These data suggest control values for normal erectile function in middle-aged men as a 70% increase in deep cavernous artery diameter, a systolic peak blood flow velocity greater than 30 cm. per second and more than 10 cc per minute of blood flow volume. With these standards the clinician may assess, design and follow treatment strategies for vasculogenic impotence.
N Fredotovich - One of the best experts on this subject based on the ideXlab platform.
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comparative study of papaverine plus phentolamine versus Prostaglandin E1 in erectile dysfunction
The Journal of Urology, 1997Co-Authors: Antoine Bechara, G Cheliz, S. V. Romano, Adolfo Casabé, N FredotovichAbstract:AbstractPurpose: We compared the efficacy and short-term adverse effects of 1 ml. 30 mg./ml. papaverine plus 0.5 mg./ml. phentolamine versus 1 ml. 30 micro g./ml. Prostaglandin E1 in patients undergoing pharmacological erection testing.Materials and Methods: A total of 60 patients (mean age 58 years) with a history of sexual erectile dysfunction longer than 6 months was randomly classified into 6 groups to be tested 1 week apart with the 2 solutions and with placebo to evaluate erection response and short-term adverse effects.Results: Of the patients tested with papaverine plus phentolamine 54% responded with erections adequate for penetration, compared to 50% of those tested with Prostaglandin E1 (p >0.05). Prolonged erection occurred in 18% of patients tested with papaverine plus phentolamine and 15% of those tested with Prostaglandin E1 (p >0.05). Pain was reported by 15 and 35% of patients, respectively (p <0.05).Conclusions: One ml. 30 mg./ml. papaverine plus 0.5 mg./ml. phentolamine has the same eff...
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Prostaglandin E1 versus mixture of Prostaglandin E1 papaverine and phentolamine in nonresponders to high papaverine plus phentolamine doses
The Journal of Urology, 1996Co-Authors: Antoine Bechara, G Cheliz, S. V. Romano, Adolfo Casabé, N FredotovichAbstract:AbstractPurpose: We evaluated the efficacy of 40 microgram/ml. Prostaglandin E1 versus a combination of 17.64 mg./ml. papaverine hydrochloride, 0.58 mg./ml. phentolamine mesylate and 5.8 microgram/ml. Prostaglandin E1 (3-drug mixture).Materials and Methods: A total of 32 patients randomly received 1 ml. of either medication by the intracavernous route. All patients had presented with erectile dysfunction longer than 6 months in duration and had failed to respond to high doses of papaverine (60 mg.) plus phentolamine (1 mg.).Results: Of 32 patients 7 (22 percent) responded to Prostaglandin E1 and 16 (50 percent) to the 3-drug mixture, achieving erections allowing penetration (grade E4 or E5, p less than 0.05). Pain was reported by 41 percent of the patients receiving Prostaglandin E1 and by 12.5 percent administered the 3-drug mixture.Conclusions: The 3-drug mixture may be regarded as more effective than Prostaglandin E1 alone in inducing an erectile response with a decreased incidence of pain.
John D Puskas - One of the best experts on this subject based on the ideXlab platform.
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equivalent eighteen hour lung preservation with low potassium dextran or euro collins solution after Prostaglandin E1 infusion
The Journal of Thoracic and Cardiovascular Surgery, 1992Co-Authors: John D Puskas, Paulo Francisco Guerreiro Cardoso, A S Slutsky, E Mayer, Shiquig Shi, G A PattersonAbstract:Improved techniques of pulmonary preservation would help alleviate the critical shortage of donor organs in lung transplantation and would improve early graft function. A previous study demonstrated that cold pulmonary artery flush with low-potassium dextran solution was superior to Euro-Collins solution in preservation of canine lung allografts stored for 12 hours when no pulmonary vasodilator was used before donor lung flush. The present study was designed to determine whether donor pretreatment with Prostaglandin E1 would affect the superiority of low-potassium dextran as a preservation solution. Prostaglandin E1 was infused (50 μg/min) in 12 donor dogs until potent vasodilatation was demonstrated. Low-pressure pulmonary artery flush (50 ml/kg) with either Euro-Collins or low-potassium dextran solution (n = 6 for each group) was performed at 4° C in a randomized, blinded fashion. Heart-lung blocks were extracted and stored at 4° C for 18 hours before left lung allografting. Inflatable cuffs were placed around each pulmonary artery, allowing independent study of the native and transplanted lungs. All 12 recipient dogs survived the 3-day assessment period. Lungs flushed and stored in Euro-Collins or low-potassium dextran solution provided equivalent gas exchange function on day 0 (arterial oxygen tension: Euro-Collins 289 ±105 mm Hg versus low-potassium dextran 265 ± 111 mm Hg; mean ± standard error of the mean) and on day 3 (Euro-Collins 516 ± 45 mm Hg versus low-potassium dextran 354 ± 77 mm Hg; p = 0.10). Mean pulmonary artery pressures in the transplanted lung were not significantly different in the Euro-Collins and low-potassium dextran groups on day 0 (21.4 ± 2 mm Hg versus 33.7 ± 5 mm Hg, respectively; p = 0.09) or on day 3 (20.2 ± 2.7 mm Hg versus 24.2 ± 5.1 mm Hg, respectively; p = 0.50). We conclude that there was no advantage of low-potassium dextran over Euro-Collins as a flush solution in this 18-hour canine single lung allograft model in which Prostaglandin E1 was administered before pulmonary artery flush. (J T horac C ardiovasc S urg 1992;104:83–9)
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equivalent eighteen hour lung preservation with low potassium dextran or euro collins solution after Prostaglandin E1 infusion
The Journal of Thoracic and Cardiovascular Surgery, 1992Co-Authors: John D Puskas, Paulo Francisco Guerreiro Cardoso, A S Slutsky, E Mayer, Shiquig Shi, G A PattersonAbstract:Improved techniques of pulmonary preservation would help alleviate the critical shortage of donor organs in lung transplantation and would improve early graft function. A previous study demonstrated that cold pulmonary artery flush with low-potassium dextran solution was superior to Euro-Collins solution in preservation of canine lung allografts stored for 12 hours when no pulmonary vasodilator was used before donor lung flush. The present study was designed to determine whether donor pretreatment with Prostaglandin E1 would affect the superiority of low-potassium dextran as a preservation solution. Prostaglandin E1 was infused (50 micrograms/min) in 12 donor dogs until potent vasodilation was demonstrated. Low-pressure pulmonary artery flush (50 ml/kg) with either Euro-Collins or low-potassium dextran solution (n = 6 for each group) was performed at 4 degrees C in a randomized, blinded fashion. Heart-lung blocks were extracted and stored at 4 degrees C for 18 hours before left lung allografting. Inflatable cuffs were placed around each pulmonary artery, allowing independent study of the native and transplanted lungs. All 12 recipient dogs survived the 3-day assessment period. Lungs flushed and stored in Euro-Collins or low-potassium dextran solution provided equivalent gas exchange function on day 0 (arterial oxygen tension: Euro-Collins 289 +/- 105 mm Hg versus low-potassium dextran 265 +/- 111 mm Hg; mean +/- standard error of the mean) and on day 3 (Euro-Collins 516 +/- 45 mm Hg versus low-potassium dextran 354 +/- 77 mm Hg; p = 0.10). Mean pulmonary artery pressures in the transplanted lung were not significantly different in the Euro-Collins and low-potassium dextran groups on day 0 (21.4 +/- 2 mm Hg versus 33.7 +/- 5 mm Hg, respectively; p = 0.09) or on day 3 (20.2 +/- 2.7 mm Hg versus 24.2 +/- 5.1 mm Hg, respectively; p = 0.50). We conclude that there was no advantage of low-potassium dextran over Euro-Collins as a flush solution in this 18-hour canine single lung allograft model in which Prostaglandin E1 was administered before pulmonary artery flush.
Jindřich Kopecek - One of the best experts on this subject based on the ideXlab platform.
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efficiency of high molecular weight backbone degradable hpma copolymer Prostaglandin E1 conjugate in promotion of bone formation in ovariectomized rats
Biomaterials, 2013Co-Authors: Huaizhong Pan, Pavla Kopeckova, Jiyuan Yang, Scott C Miller, Monika Sima, Jindřich KopecekAbstract:Multiblock, high molecular weight, linear, backbone degradable HPMA copolymer-Prostaglandin E1 (PGE1) conjugate has been synthesized by RAFT polymerization mediated by a new bifunctional chain transfer agent (CTA), which contains an enzymatically degradable oligopeptide sequence flanked by two dithiobenzoate groups, followed by postpolymerization aminolysis and thiol-ene chain extension. The multiblock conjugate contains Asp8 as the bone targeting moiety and enzymatically degradable bonds in the polymer backbone; in vivo degradation produces cleavage products that are below the renal threshold. Using an ovariectomized (OVX) rat model, the accumulation in bone and efficacy to promote bone formation was evaluated; low molecular weight conjugates served as control. The results indicated a higher accumulation in bone, greater enhancement of bone density, and higher plasma osteocalcin levels for the backbone degradable conjugate.
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water soluble hpma copolymer Prostaglandin E1 conjugates containing a cathepsin k sensitive spacer
Journal of Drug Targeting, 2006Co-Authors: Huaizhong Pan, Pavla Kopeckova, Dong Wang, Jiyuan Yang, Scott C Miller, Jindřich KopecekAbstract:A novel bone targeting, N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer based, Prostaglandin E1 (PGE1) delivery system was designed, synthesized and characterized. PGE1 was bound to the polymer backbone via a spacer, composed of a cathepsin K sensitive tetrapeptide (Gly-Gly-Pro-Nle) and a self-eliminating 4-aminobenzyl alcohol structure. The HPMA copolymer conjugates were prepared by photo–initiated free radical copolymerization of HPMA, PGE1-containing macromonomer, and optionally a comonomer containing a reactive p-nitrophenyl ester group. The latter group was used as attachment points for the d-aspartic acid octapeptide targeting moieties. Incubation of the PGE1-containing macromonomer and HPMA copolymer-PGE1 conjugates with cathepsin K resulted in release of unmodified PGE1. The rate of release depended on the composition of the conjugate. The higher the PGE1 content in the conjugate, the slower the PGE1 release. This appeared to be the result of association of hydrophobic side-chains in aqueous me...
