The Experts below are selected from a list of 17385 Experts worldwide ranked by ideXlab platform
Cindy K Miranti - One of the best experts on this subject based on the ideXlab platform.
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tetraspanin kai1 cd82 suppresses invasion by inhibiting integrin dependent crosstalk with c met receptor and src kinases
Oncogene, 2006Co-Authors: S C Sridhar, Cindy K MirantiAbstract:KAI1/CD82, a tetraspanin protein, was first identified as a metastasis suppressor in Prostate cancer. How loss of CD82 expression promotes cancer metastasis is unknown. Restoration of CD82 expression to physiological levels in the metastatic Prostate Cell Line PC3 inhibits integrin-mediated Cell migration and invasion, but does not affect integrin expression. Integrin-dependent activation of the receptor kinase c-Met is dramatically reduced in CD82-expressing Cells, as is c-Met activation by its ligand HGF/SF. CD82 expression also reduced integrin-induced activation and phosphorylation of the cytoplasmic tyrosine kinase Src, and its downstream substrates p130Cas and FAK Y861. Inhibition of c-Met expression or Src kinase function reduced matrigel invasion of PC3 Cells to the same extent as CD82 expression. These data indicate that CD82 functions to suppress integrin-induced invasion by regulating signaling to c-Met and Src kinases, and suggests that CD82 loss may promote metastasis by removing a negative regulator of c-Met and Src signaling.
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Tetraspanin KAI1/CD82 suppresses invasion by inhibiting integrin-dependent crosstalk with c-Met receptor and Src kinases.
Oncogene, 2005Co-Authors: Suganthi Sridhar, Cindy K MirantiAbstract:KAI1/CD82, a tetraspanin protein, was first identified as a metastasis suppressor in Prostate cancer. How loss of CD82 expression promotes cancer metastasis is unknown. Restoration of CD82 expression to physiological levels in the metastatic Prostate Cell Line PC3 inhibits integrin-mediated Cell migration and invasion, but does not affect integrin expression. Integrin-dependent activation of the receptor kinase c-Met is dramatically reduced in CD82-expressing Cells, as is c-Met activation by its ligand HGF/SF. CD82 expression also reduced integrin-induced activation and phosphorylation of the cytoplasmic tyrosine kinase Src, and its downstream substrates p130Cas and FAK Y861. Inhibition of c-Met expression or Src kinase function reduced matrigel invasion of PC3 Cells to the same extent as CD82 expression. These data indicate that CD82 functions to suppress integrin-induced invasion by regulating signaling to c-Met and Src kinases, and suggests that CD82 loss may promote metastasis by removing a negative regulator of c-Met and Src signaling.
Fioretta Palombi - One of the best experts on this subject based on the ideXlab platform.
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The Adherent/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response
Infection and immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta Palombi, Paola De CesarisAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
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the adherent invasive escherichia coli strain lf82 invades and persists in human Prostate Cell Line rwpe 1 activating a strong inflammatory response
Infection and Immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta PalombiAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
Joy L. Ware - One of the best experts on this subject based on the ideXlab platform.
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Proteomic analysis of the tumorigenic human Prostate Cell Line M12 after microCell‐mediated transfer of chromosome 19 demonstrates reduction of vimentin
ELECTROPHORESIS, 2003Co-Authors: Xuhui Liu, Zendra E. Zehner, Colleen Jackson-cook, Joy L. WareAbstract:Critical alterations in proteins that accompany or control the aggressiveness of human Prostate cancers remain poorly defined. Previously we demonstrated that the highly tumorigenic, metastatic human Prostate Cell Line M12 was converted to a slow growing, poorly tumorigenic Cell Line by introduction of an intact human chromosome 19, generating the M12 (F6) hybrid Cells. The objective of this report was to identify changes in the protein profile of these M12(F6) microCell hybrid Cells. A combination of two-dimensional gel electrophoresis and matrix assisted laser desorption-time of flight-mass spectroscopy was used to compare proteins made by these two Cell Lines. No consistently increased proteins were identified. However, seven proteins were reproducibly reduced more than twofold: vimentin, hsp90, ATP synthase, 26S protease regulatory subunit, heterogeneous nuclear ribonucleoprotein, T-Complex protein 1 β, and α-1 tubulin. The striking reduction in vimentin protein was accompanied by significantly decreased vimentin mRNA, revealed by Northern blotting. Our findings implicate reduced vimentin in the conversion of these tumorigenic Prostate epithelial Cells into slow growing, less aggressive Cells. These studies demonstrate that application of proteomic analysis to specific problems in an experimental context can yield biologically relevant information about the Prostate cancer Cell phenotype.
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Proteomic analysis of the tumorigenic human Prostate Cell Line M12 after microCell-mediated transfer of chromosome 19 demonstrates reduction of vimentin.
Electrophoresis, 2003Co-Authors: Xuhui Liu, Zendra E. Zehner, Colleen Jackson-cook, Joy L. WareAbstract:Critical alterations in proteins that accompany or control the aggressiveness of human Prostate cancers remain poorly defined. Previously we demonstrated that the highly tumorigenic, metastatic human Prostate Cell Line M12 was converted to a slow growing, poorly tumorigenic Cell Line by introduction of an intact human chromosome 19, generating the M12 (F6) hybrid Cells. The objective of this report was to identify changes in the protein profile of these M12(F6) microCell hybrid Cells. A combination of two-dimensional gel electrophoresis and matrix assisted laser desorption-time of flight-mass spectroscopy was used to compare proteins made by these two Cell Lines. No consistently increased proteins were identified. However, seven proteins were reproducibly reduced more than twofold: vimentin, hsp90, ATP synthase, 26S protease regulatory subunit, heterogeneous nuclear ribonucleoprotein, T-Complex protein 1 beta, and alpha-1 tubulin. The striking reduction in vimentin protein was accompanied by significantly decreased vimentin mRNA, revealed by Northern blotting. Our findings implicate reduced vimentin in the conversion of these tumorigenic Prostate epithelial Cells into slow growing, less aggressive Cells. These studies demonstrate that application of proteomic analysis to specific problems in an experimental context can yield biologically relevant information about the Prostate cancer Cell phenotype.
Guido Gambara - One of the best experts on this subject based on the ideXlab platform.
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The Adherent/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response
Infection and immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta Palombi, Paola De CesarisAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
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the adherent invasive escherichia coli strain lf82 invades and persists in human Prostate Cell Line rwpe 1 activating a strong inflammatory response
Infection and Immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta PalombiAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
Maria Pia Conte - One of the best experts on this subject based on the ideXlab platform.
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The Adherent/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response
Infection and immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta Palombi, Paola De CesarisAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
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the adherent invasive escherichia coli strain lf82 invades and persists in human Prostate Cell Line rwpe 1 activating a strong inflammatory response
Infection and Immunity, 2016Co-Authors: Maria Pia Conte, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Cecilia Ambrosi, Mauro Nicoletti, Carlo Zagaglia, Guido Gambara, Fioretta PalombiAbstract:Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect Prostate Cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 Prostate Cell Line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within Prostate epithelial Cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host Cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 Cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-Cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
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Features of uropathogenic Escherichia coli able to invade a Prostate Cell Line.
The new microbiologica, 2016Co-Authors: Catia Longhi, Marta Aleandri, Massimiliano Marazzato, Antonietta Lucia Conte, Antonella Comanducci, Anna Riccioli, Elio Ziparo, Maria Stefania Lepanto, Paola Goldoni, Maria Pia ConteAbstract:RWPE-1 normal Prostate Cells were tested as an experimental model for adhesion/invasion assays by genotypically and phenotypically characterized community uropathogenic strains of Escherichia coli (UPEC), a frequent cause of urinary tract infections (UTIs) and significant etiologic agent also in bacterial prostatitis. Adhesive ability and strong biofilm production was significantly associated with the bacterial invasive phenotype. Invasive strains derived mainly from male and pediatric patients. This study suggests that such a Cell model could usefully integrate other available methods of urovirulence analysis, to deepen knowledge on the bacterial interaction with host Cells.
