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John M Krochta - One of the best experts on this subject based on the ideXlab platform.
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swelling and Protein Absorption desorption of thermo sensitive lactitol based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
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Swelling and Protein Absorption/desorption of thermo-sensitive lactitol- based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
D Chacon - One of the best experts on this subject based on the ideXlab platform.
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swelling and Protein Absorption desorption of thermo sensitive lactitol based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
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Swelling and Protein Absorption/desorption of thermo-sensitive lactitol- based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
Mark J Kurth - One of the best experts on this subject based on the ideXlab platform.
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swelling and Protein Absorption desorption of thermo sensitive lactitol based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
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Swelling and Protein Absorption/desorption of thermo-sensitive lactitol- based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
You Lo Hsieh - One of the best experts on this subject based on the ideXlab platform.
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swelling and Protein Absorption desorption of thermo sensitive lactitol based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
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Swelling and Protein Absorption/desorption of thermo-sensitive lactitol- based polyether polyol hydrogels
Polymer, 2000Co-Authors: D Chacon, You Lo Hsieh, Mark J Kurth, John M KrochtaAbstract:Abstract A series of thermo-sensitive hydrogels has been prepared from reactions of acylated poly(ethylene glycol) bis(carboxymethyl) ether (PEGBCOCl) (M n =600 dalton ) with lactitol-based polyether polyols (LPEPs). These LPEP hydrogels swelled extensively in water at neutral pH and their swelling behaviors depended strongly on the PEGBCOCl:LPEP molar ratios or extent of PEGBCOCl crosslinking. A maximum swelling of 81 fold was observed on hydrogels formed with LPEP (M n =4055 dalton ) at a PEGBCOCl:LPEP molar ratio of 4.25. At temperatures above 25°C, the hydrogels exhibited a phase transition and collapsed, expelling water. These super-absorbent hydrogels were stable under acidic conditions, but were sensitive to base hydrolysis. Enzyme Proteins, i.e. lipase, were incorporated in the hydrogels (0.4–1.2 mg/g) by immersing the collapsed hydrogels in the Protein solutions at 25°C. Protein desorption at 40°C occurred rapidly with over 90% of Protein released during the first hour. The extent of Protein desorption was similar among hydrogels with varying levels of absorbed Proteins. The release of the lipase Protein molecules is due to the structural collapse of the hydrogels and is not diffusion controlled.
Li Jieshou - One of the best experts on this subject based on the ideXlab platform.
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Serum citrulline is a simple quantitative marker for small intestinal enterocytes mass and Absorption function in short bowel patients.
The Journal of surgical research, 2005Co-Authors: Gong Jian-feng, Zhu Weiming, Li Ning, Liu Fangnan, Luo Nan, Li JieshouAbstract:Background To investigate the clinical significance of serum citrulline in evaluating the remnant small bowel enterocytes mass and absorptive function in short bowel (SB) patients. Materials and methods Serum citrulline concentrations were determined using high-performance liquid chromatography (HPLC) in 22 SBS patients and 33 healthy controls. Five-hour urine D-xylose excretion and digestive Protein Absorption were measured using HPLC and micro-Kjeldahl method, respectively. Small bowel length and surface area were assessed on X-ray radiograph. Correlations between serum citrulline levels and small bowel length, small bowel surface, and nutritional substrate digestive Absorption percentage were analyzed. For six patients receiving bowel rehabilitation therapy, serum citrulline, D-xylose excretion, and intestinal Protein Absorption were measured pre- and immediately postmanagement, and their correlations were analyzed. Results Serum citrulline levels were significantly lower in SB patients compared with healthy controls. In SB patients, they correlated well with remnant small bowel length (r = 0.82, P < 0.001), surface area (r = 0.86, P < 0.001), 5-h urine D-xylose excretion (r = 0.56, P = 0.007), and digestive Protein Absorption (r = 0.48, P = 0.046). The increased percentage of serum citrulline level in six patients receiving rehabilitation therapy followed a trend of correlating with that of intestinal Protein Absorption (r = 0.79, P = 0.063) and urine D-xylose excretion (r = 0.81, P = 0.053). Conclusions In patients with short bowel syndrome, serum citrulline is a simple and accurate biomarker for the severity of intestinal failure and may be a candidate marker for the gut-trophic effects of bowel rehabilitation therapies.
