The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform
Jack R. Leonards - One of the best experts on this subject based on the ideXlab platform.
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Simplified Technic for the Determination of Serum Protein-Bound Iodine
2016Co-Authors: Louise W. Faulkner, Richard P. Levy, Jack R. LeonardsAbstract:A simplified technic for the determination of serum Protein-Bound Iodine (PBI) is presented. Endogenouslylabeled 1131 PBI is completely recovered. The technic is basedon the method of Barker et al. (1) and includes the following modifications: The number of washesof the precipitated serum Proteins are decreased. Unashed iodide standards are used in a sodium carbonate solution rather than an internal standard. A temperature-correction formula is employed to allow the ceric-arsenite-iodide reaction to be carried out at room temperature, and a stable cerate color is producedwith brucine sulfate so that the colorimetry is carried out with greater convenience. The serum PBI concentrations are read directly from a graph, which simplifies the calculations. INTEREST in the technic of determining serum Protein-Bound Iodine (PBI) has continued to increase since this test was recognized as a valuable tool in the diagnosis and management of thyroid disease. Although most of the commonly employed methods for this deter-mination are considered to be accurate and reproducible, they are not completely satisfactory for busy clinical and research labora-tories because they are time consuming and require constant atten-tion by the analyst. In this laboratory, several published methods were tried. The method of Barker et at. (1) employed the internal standard which not only limited the number of samples that could be completed at one time, but involved tedious calculations. Grossman and Grossman (2) presented a method for the production of a stable cerate color with brucine sulfate. Their modification increased the number o
Muzaffer Selçuk - One of the best experts on this subject based on the ideXlab platform.
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EFFECTS OF CHRONIC FLUOROSIS ON THYROXINE, TRIIODOTHYRONINE, AND Protein-Bound Iodine IN COWS
2005Co-Authors: Ali Çinar, Muzaffer SelçukAbstract:SUMMARY: This study was conducted to evaluate the effects of chronic fluorosis in cows on their blood serum levels of thyroxine (T 4 ), triiodothyronine (T 3 ), and Protein-Bound Iodine (PBI). Data collected from twenty cows with chronic fluorosis in the Tendurek Mountain region (altitude about 2000 m) in East Anatolia, Turkey, were compared with data from ten healthy cows from the Van region (altitude 1700 m). Statistically significant differences (p
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effects of chronic fluorosis on thyroxine triiodothyronine and Protein Bound Iodine in cows
2005Co-Authors: Ali Çinar, Muzaffer SelçukAbstract:SUMMARY: This study was conducted to evaluate the effects of chronic fluorosis in cows on their blood serum levels of thyroxine (T 4 ), triiodothyronine (T 3 ), and Protein-Bound Iodine (PBI). Data collected from twenty cows with chronic fluorosis in the Tendurek Mountain region (altitude about 2000 m) in East Anatolia, Turkey, were compared with data from ten healthy cows from the Van region (altitude 1700 m). Statistically significant differences (p<0.05) between the serum values in the fluorotic cows and the controls were found: 5.7±0.48 vs 3.7±0.45 µg/dL for T4, 1.53±0.038 vs 0.97±0.051 ng/mL for T 3 , and 3.8±0.29 vs 2.6±0.23 µg/dL for PBI. Hypothyroidism was therefore evident in the cows with chronic fluorosis.
Louise W. Faulkner - One of the best experts on this subject based on the ideXlab platform.
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Simplified Technic for the Determination of Serum Protein-Bound Iodine
2016Co-Authors: Louise W. Faulkner, Richard P. Levy, Jack R. LeonardsAbstract:A simplified technic for the determination of serum Protein-Bound Iodine (PBI) is presented. Endogenouslylabeled 1131 PBI is completely recovered. The technic is basedon the method of Barker et al. (1) and includes the following modifications: The number of washesof the precipitated serum Proteins are decreased. Unashed iodide standards are used in a sodium carbonate solution rather than an internal standard. A temperature-correction formula is employed to allow the ceric-arsenite-iodide reaction to be carried out at room temperature, and a stable cerate color is producedwith brucine sulfate so that the colorimetry is carried out with greater convenience. The serum PBI concentrations are read directly from a graph, which simplifies the calculations. INTEREST in the technic of determining serum Protein-Bound Iodine (PBI) has continued to increase since this test was recognized as a valuable tool in the diagnosis and management of thyroid disease. Although most of the commonly employed methods for this deter-mination are considered to be accurate and reproducible, they are not completely satisfactory for busy clinical and research labora-tories because they are time consuming and require constant atten-tion by the analyst. In this laboratory, several published methods were tried. The method of Barker et at. (1) employed the internal standard which not only limited the number of samples that could be completed at one time, but involved tedious calculations. Grossman and Grossman (2) presented a method for the production of a stable cerate color with brucine sulfate. Their modification increased the number o
Ali Çinar - One of the best experts on this subject based on the ideXlab platform.
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EFFECTS OF CHRONIC FLUOROSIS ON THYROXINE, TRIIODOTHYRONINE, AND Protein-Bound Iodine IN COWS
2005Co-Authors: Ali Çinar, Muzaffer SelçukAbstract:SUMMARY: This study was conducted to evaluate the effects of chronic fluorosis in cows on their blood serum levels of thyroxine (T 4 ), triiodothyronine (T 3 ), and Protein-Bound Iodine (PBI). Data collected from twenty cows with chronic fluorosis in the Tendurek Mountain region (altitude about 2000 m) in East Anatolia, Turkey, were compared with data from ten healthy cows from the Van region (altitude 1700 m). Statistically significant differences (p
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effects of chronic fluorosis on thyroxine triiodothyronine and Protein Bound Iodine in cows
2005Co-Authors: Ali Çinar, Muzaffer SelçukAbstract:SUMMARY: This study was conducted to evaluate the effects of chronic fluorosis in cows on their blood serum levels of thyroxine (T 4 ), triiodothyronine (T 3 ), and Protein-Bound Iodine (PBI). Data collected from twenty cows with chronic fluorosis in the Tendurek Mountain region (altitude about 2000 m) in East Anatolia, Turkey, were compared with data from ten healthy cows from the Van region (altitude 1700 m). Statistically significant differences (p<0.05) between the serum values in the fluorotic cows and the controls were found: 5.7±0.48 vs 3.7±0.45 µg/dL for T4, 1.53±0.038 vs 0.97±0.051 ng/mL for T 3 , and 3.8±0.29 vs 2.6±0.23 µg/dL for PBI. Hypothyroidism was therefore evident in the cows with chronic fluorosis.
Richard P. Levy - One of the best experts on this subject based on the ideXlab platform.
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Simplified Technic for the Determination of Serum Protein-Bound Iodine
2016Co-Authors: Louise W. Faulkner, Richard P. Levy, Jack R. LeonardsAbstract:A simplified technic for the determination of serum Protein-Bound Iodine (PBI) is presented. Endogenouslylabeled 1131 PBI is completely recovered. The technic is basedon the method of Barker et al. (1) and includes the following modifications: The number of washesof the precipitated serum Proteins are decreased. Unashed iodide standards are used in a sodium carbonate solution rather than an internal standard. A temperature-correction formula is employed to allow the ceric-arsenite-iodide reaction to be carried out at room temperature, and a stable cerate color is producedwith brucine sulfate so that the colorimetry is carried out with greater convenience. The serum PBI concentrations are read directly from a graph, which simplifies the calculations. INTEREST in the technic of determining serum Protein-Bound Iodine (PBI) has continued to increase since this test was recognized as a valuable tool in the diagnosis and management of thyroid disease. Although most of the commonly employed methods for this deter-mination are considered to be accurate and reproducible, they are not completely satisfactory for busy clinical and research labora-tories because they are time consuming and require constant atten-tion by the analyst. In this laboratory, several published methods were tried. The method of Barker et at. (1) employed the internal standard which not only limited the number of samples that could be completed at one time, but involved tedious calculations. Grossman and Grossman (2) presented a method for the production of a stable cerate color with brucine sulfate. Their modification increased the number o
