The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Chengxi Cao - One of the best experts on this subject based on the ideXlab platform.
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retardation signal for fluorescent determination of total Protein Content via rapid and sensitive chip moving reaction boundary electrophoretic titration
Analytical Chemistry, 2014Co-Authors: Houyu Wang, Yongting Shi, Jian Yan, Jingyu Dong, Hua Xiao, Haiyang Xie, Liuyin Fan, Chengxi CaoAbstract:A novel concept and theory of moving reaction boundary (MRB) retardation signal (RMRB) was advanced for determination of total Protein Content via MRB electrophoretic titration (MRBET). The theoretical results revealed that the retardation extent of boundary displacment, viz., the RMRB value, was as a function of Protein Content. Thus, the RMRB value of a sample could be used to determine its total Protein Content according to the relevant calibration curve. To demonstrate the concept and theoretical results, a novel microdevice was designed for the relevant experiments of MRBET. The microdevice has 30 identical work cells, each of which is composed of five ultrashort single microchannels (5 mm). In the microdevice, fluorescein isothiocyanate (FITC) was used to denote MRB motion and RMRB value for the first time, the polyacrylamide gel (PAG) containing Protein sample was photopolymerized in microchannels, and the MRB was created with acid or alkali and target Protein sample. As compared to the classic Kje...
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a visual detection of Protein Content based on titration of moving reaction boundary electrophoresis
Analytica Chimica Acta, 2013Co-Authors: Houyu Wang, Liuyin Fan, Chengye Guo, Chengang Guo, Lei Zhang, Chengxi CaoAbstract:Abstract A visual electrophoretic titration method was firstly developed from the concept of moving reaction boundary (MRB) for Protein Content analysis. In the developed method, when the voltage was applied, the hydroxide ions in the cathodic vessel moved towards the anode, and neutralized the carboxyl groups of Protein immobilized via highly cross-linked polyacrylamide gel (PAG), generating a MRB between the alkali and the immobilized Protein. The boundary moving velocity (VMRB) was as a function of Protein Content, and an acid–base indicator was used to denote the boundary displacement. As a proof of concept, standard model Proteins and biological samples were chosen for the experiments to study the feasibility of the developed method. The experiments revealed that good linear calibration functions between VMRB and Protein Content (correlation coefficients R > 0.98). The experiments further demonstrated the following merits of developed method: (1) weak influence of non-Protein nitrogen additives (e.g., melamine) adulterated in Protein samples, (2) good agreement with the classic Kjeldahl method (R = 0.9945), (3) fast measuring speed in total Protein analysis of large samples from the same source, and (4) low limit of detection (0.02–0.15 mg mL−1 for Protein Content), good precision (R.S.D. of intra-day less than 1.7% and inter-day less than 2.7%), and high recoveries (105–107%).
Irene Biancarosa - One of the best experts on this subject based on the ideXlab platform.
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amino acid composition Protein Content and nitrogen to Protein conversion factors of 21 seaweed species from norwegian waters
Journal of Applied Phycology, 2017Co-Authors: Irene Biancarosa, Marit Espe, Christian Guido Bruckner, Svenja Heesch, Nina S Liland, Rune Waagbo, Bente E Torstensen, Erikjan LockAbstract:The Norwegian seaweed industry is expanding and there is a need for accurate estimates of Protein Content of seaweed species from Norwegian waters. A solid method to calculate Protein Content is through the sum of the proteomic amino acids; however, it can be expensive and beyond the capacities of many laboratories. The most commonly used method to quantify Protein is based on the assessment of crude Protein from overall nitrogen Content, using the traditional nitrogen-to-Protein conversion factor of 6.25. However, this approach can be inaccurate when applied to seaweeds, often resulting in an overestimation of their Protein Content. Specific nitrogen-to-Protein conversion factors, calculated from amino acid composition and total nitrogen, give a more reliable Protein quantification in seaweeds. However, no such factors are available for species from Norwegian waters. This study was designed to characterize the amino acid composition of 21 seaweed species from Norwegian waters and use the amino acid data to estimate Protein Contents of the seaweeds. Crude Protein analysis (nitrogen × 6.25) was performed and resulted in overestimation (18–44 %) of the Protein Content compared to the sum of proteomic amino acids. Specific nitrogen-to-Protein conversion factors, calculated for each species, ranged from 3.53 ± 0.1 to 5.13 ± 0.1. This study provides nutritional data on Norwegian seaweeds, covering a relatively wide range of species. Moreover, it is the first study to assess nitrogen-to-Protein conversion factors on such species.
Siti K Maznah - One of the best experts on this subject based on the ideXlab platform.
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effect of soaking in potassium hydroxide solution on the curing tensile properties and extractable Protein Content of natural rubber latex films
Polymer Testing, 2008Co-Authors: Siti K Maznah, Idris Hanafi, M E Azhar, Amirah Baharin, Mas Haris Mas Rosemal HakimAbstract:Natural rubber latex films were soaked in potassium hydroxide solution of various pH. The effects of soaking on the crosslink density, extractable Protein Content and tensile properties of the films were investigated. Potassium hydroxide solution was believed to be effective in reducing extractable Protein Content by leaching out the extractable Protein from natural rubber latex films. Results showed that crosslink density and the extractable Protein Content of the films reduced with increasing pH of the KOH solution. The tensile properties, except elongation at break, reduce when pH of KOH solution is above 12. Extractable Protein Content was reduced especially when the pH of the solution was greater than 12.0.
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effect of acid treatment on extractable Protein Content crosslink density and tensile properties of natural rubber latex films
Polymer Testing, 2008Co-Authors: Siti K Maznah, Idris Hanafi, M E Azhar, Amirah Baharin, M Mas Rosmal H HakimAbstract:Abstract The effect of acid treatment on the extractable Protein Content and tensile properties of natural rubber latex films was studied. The main aim was to introduce an alternative method to tackle the Protein allergy in natural rubber latex products. Natural rubber latex was treated with various amounts of 7.0% hydrochloric acid to get pH values in the range of 2.0–9.0 at 70 °C. Results showed that the extractable Protein Content of the films reduced with reducing pH. The crosslink density, tensile strength and the elongation at break of acid treated films were slightly lower than that of the untreated films. The moduli at 100% and 300% elongation of the films were not affected by acid treatment. Acid treatment was found effective in reducing extractable Protein Content in natural rubber latex films.
M Mas Rosmal H Hakim - One of the best experts on this subject based on the ideXlab platform.
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effect of acid treatment on extractable Protein Content crosslink density and tensile properties of natural rubber latex films
Polymer Testing, 2008Co-Authors: Siti K Maznah, Idris Hanafi, M E Azhar, Amirah Baharin, M Mas Rosmal H HakimAbstract:Abstract The effect of acid treatment on the extractable Protein Content and tensile properties of natural rubber latex films was studied. The main aim was to introduce an alternative method to tackle the Protein allergy in natural rubber latex products. Natural rubber latex was treated with various amounts of 7.0% hydrochloric acid to get pH values in the range of 2.0–9.0 at 70 °C. Results showed that the extractable Protein Content of the films reduced with reducing pH. The crosslink density, tensile strength and the elongation at break of acid treated films were slightly lower than that of the untreated films. The moduli at 100% and 300% elongation of the films were not affected by acid treatment. Acid treatment was found effective in reducing extractable Protein Content in natural rubber latex films.
Erikjan Lock - One of the best experts on this subject based on the ideXlab platform.
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amino acid composition Protein Content and nitrogen to Protein conversion factors of 21 seaweed species from norwegian waters
Journal of Applied Phycology, 2017Co-Authors: Irene Biancarosa, Marit Espe, Christian Guido Bruckner, Svenja Heesch, Nina S Liland, Rune Waagbo, Bente E Torstensen, Erikjan LockAbstract:The Norwegian seaweed industry is expanding and there is a need for accurate estimates of Protein Content of seaweed species from Norwegian waters. A solid method to calculate Protein Content is through the sum of the proteomic amino acids; however, it can be expensive and beyond the capacities of many laboratories. The most commonly used method to quantify Protein is based on the assessment of crude Protein from overall nitrogen Content, using the traditional nitrogen-to-Protein conversion factor of 6.25. However, this approach can be inaccurate when applied to seaweeds, often resulting in an overestimation of their Protein Content. Specific nitrogen-to-Protein conversion factors, calculated from amino acid composition and total nitrogen, give a more reliable Protein quantification in seaweeds. However, no such factors are available for species from Norwegian waters. This study was designed to characterize the amino acid composition of 21 seaweed species from Norwegian waters and use the amino acid data to estimate Protein Contents of the seaweeds. Crude Protein analysis (nitrogen × 6.25) was performed and resulted in overestimation (18–44 %) of the Protein Content compared to the sum of proteomic amino acids. Specific nitrogen-to-Protein conversion factors, calculated for each species, ranged from 3.53 ± 0.1 to 5.13 ± 0.1. This study provides nutritional data on Norwegian seaweeds, covering a relatively wide range of species. Moreover, it is the first study to assess nitrogen-to-Protein conversion factors on such species.
