The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform
Kazumitsu Ueda - One of the best experts on this subject based on the ideXlab platform.
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effect of cisplatin treatment on speckled distribution of a serine arginine rich nuclear Protein Crop luc7a
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear Protein Crop/Luc7A.
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Crop luc7a a novel serine arginine rich nuclear Protein isolated from cisplatin resistant cell line1
FEBS Letters, 2000Co-Authors: Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Kouji Umehara, Yoshitake Terano, Kazumitsu UedaAbstract:A novel putative SR Protein, designated cisplatin resistance-associated overexpressed Protein (Crop), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of Crop contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of Crop is about 2–3-fold higher in mRNA and Protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. Crop is the human homologue of yeast Luc7p, which is supposed to be involved in 5′-splice site recognition and is essential for vegetative growth.
Yoichi Nishii - One of the best experts on this subject based on the ideXlab platform.
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effect of cisplatin treatment on speckled distribution of a serine arginine rich nuclear Protein Crop luc7a
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear Protein Crop/Luc7A.
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Crop luc7a a novel serine arginine rich nuclear Protein isolated from cisplatin resistant cell line1
FEBS Letters, 2000Co-Authors: Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Kouji Umehara, Yoshitake Terano, Kazumitsu UedaAbstract:A novel putative SR Protein, designated cisplatin resistance-associated overexpressed Protein (Crop), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of Crop contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of Crop is about 2–3-fold higher in mRNA and Protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. Crop is the human homologue of yeast Luc7p, which is supposed to be involved in 5′-splice site recognition and is essential for vegetative growth.
Noriyuki Kioka - One of the best experts on this subject based on the ideXlab platform.
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effect of cisplatin treatment on speckled distribution of a serine arginine rich nuclear Protein Crop luc7a
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear Protein Crop/Luc7A.
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Crop luc7a a novel serine arginine rich nuclear Protein isolated from cisplatin resistant cell line1
FEBS Letters, 2000Co-Authors: Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Kouji Umehara, Yoshitake Terano, Kazumitsu UedaAbstract:A novel putative SR Protein, designated cisplatin resistance-associated overexpressed Protein (Crop), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of Crop contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of Crop is about 2–3-fold higher in mRNA and Protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. Crop is the human homologue of yeast Luc7p, which is supposed to be involved in 5′-splice site recognition and is essential for vegetative growth.
Yoshiyuki Kakehi - One of the best experts on this subject based on the ideXlab platform.
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effect of cisplatin treatment on speckled distribution of a serine arginine rich nuclear Protein Crop luc7a
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear Protein Crop/Luc7A.
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Crop luc7a a novel serine arginine rich nuclear Protein isolated from cisplatin resistant cell line1
FEBS Letters, 2000Co-Authors: Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Kouji Umehara, Yoshitake Terano, Kazumitsu UedaAbstract:A novel putative SR Protein, designated cisplatin resistance-associated overexpressed Protein (Crop), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of Crop contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of Crop is about 2–3-fold higher in mRNA and Protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. Crop is the human homologue of yeast Luc7p, which is supposed to be involved in 5′-splice site recognition and is essential for vegetative growth.
Masaki Morishima - One of the best experts on this subject based on the ideXlab platform.
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effect of cisplatin treatment on speckled distribution of a serine arginine rich nuclear Protein Crop luc7a
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
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Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear Protein Crop/Luc7A.
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hiroshi Umehara, Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Jun Koizumi, Masatoshi Hagiwara, Kazumitsu UedaAbstract:Abstract The C-half of cisplatin resistance-associated overexpressed Protein (Crop), an SR-related Protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of Crop in cellular localization, subnuclear localization, and Protein–Protein interaction. Crop fused with green fluorescent Protein, GFP–Crop, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that Crop interacted with SF2/ASF, an SR Protein involved in RNA splicing, as well as Crop itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the Protein–Protein interaction. Crop was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP–Crop was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR Proteins including Crop.
-
Crop luc7a a novel serine arginine rich nuclear Protein isolated from cisplatin resistant cell line1
FEBS Letters, 2000Co-Authors: Yoichi Nishii, Masaki Morishima, Yoshiyuki Kakehi, Noriyuki Kioka, Teruo Amachi, Kouji Umehara, Yoshitake Terano, Kazumitsu UedaAbstract:A novel putative SR Protein, designated cisplatin resistance-associated overexpressed Protein (Crop), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of Crop contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of Crop is about 2–3-fold higher in mRNA and Protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. Crop is the human homologue of yeast Luc7p, which is supposed to be involved in 5′-splice site recognition and is essential for vegetative growth.
