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Jason A Burdick - One of the best experts on this subject based on the ideXlab platform.
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Local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell–extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies—proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels—mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair. The extracellular matrix surrounding cells plays a significant role in their behaviour. The spreading, mechanosensing and differentiation of mesenchymal stem cells are shown to be dependent on the early Deposition and remodelling of local nascent Proteins within degradable and viscoelastic hydrogels.
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local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell-extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies-proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels-mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair.
Claudia Loebel - One of the best experts on this subject based on the ideXlab platform.
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Local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell–extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies—proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels—mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair. The extracellular matrix surrounding cells plays a significant role in their behaviour. The spreading, mechanosensing and differentiation of mesenchymal stem cells are shown to be dependent on the early Deposition and remodelling of local nascent Proteins within degradable and viscoelastic hydrogels.
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local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell-extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies-proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels-mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair.
Gerald G Fuller - One of the best experts on this subject based on the ideXlab platform.
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the influence of Protein Deposition on contact lens tear film stability
Colloids and Surfaces B: Biointerfaces, 2019Co-Authors: Noelle I Rabiah, Charles W Scales, Gerald G FullerAbstract:Abstract When contact lenses (CLs) are worn, they are subject to Deposition of the surrounding biomolecules found in the tear film (TF) of the eye. There is a correlation between Protein Deposition on CLs and feelings of discomfort in patients, but it has not been well understood if these feelings of discomfort arise solely from immunogenic reactions to the Protein deposits or a physical instability of the tear film on Protein-fouled CLs. This study compared two hydrogel CLs: etafilcon A (polyhydroxyethylmethacrylate-based hydrogel) and senofilcon A (silicone hydrogel with internal wetting agent) to elucidate how lysozyme and mucin sorption affect the wettability of CLs and understand the potential impact on TF stability in vivo. Here, we use “wettability” to refer to the stability of a film of phosphate buffered saline on the CL surface. A custom-built platform was used to conduct experiments that monitored the stability of phosphate-buffered saline (PBS) and artificial tear solution (ATS) on clean and fouled CLs. PBS was more stable (wettable) on etafilcon A than senofilcon A, and both CLs showed increased wettability after Protein-fouling. However, surface wettability in PBS did not correlate with the stability of ATS on the CLs. The viscoelastic interface of ATS slowed drainage, making evaporation the primary thinning factor, in addition to the presence of a disjoining pressure that stabilized the thin film. From this, we conclude that Protein Deposition increases CL wettability, but does not alter tear film stability and we predict that CL susceptibility to evaporation is a better predictor of TF stability than wettability.
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corneal cell adhesion to contact lens hydrogel materials enhanced via tear film Protein Deposition
PLOS ONE, 2014Co-Authors: Claire M. Elkins, Qin M Qi, Gerald G FullerAbstract:Tear film Protein Deposition on contact lens hydrogels has been well characterized from the perspective of bacterial adhesion and viability. However, the effect of Protein Deposition on lens interactions with the corneal epithelium remains largely unexplored. The current study employs a live cell rheometer to quantify human corneal epithelial cell adhesion to soft contact lenses fouled with the tear film Protein lysozyme. PureVision balafilcon A and AirOptix lotrafilcon B lenses were soaked for five days in either phosphate buffered saline (PBS), borate buffered saline (BBS), or Sensitive Eyes Plus Saline Solution (Sensitive Eyes), either pure or in the presence of lysozyme. Treated contact lenses were then contacted to a live monolayer of corneal epithelial cells for two hours, after which the contact lens was sheared laterally. The apparent cell monolayer relaxation modulus was then used to quantify the extent of cell adhesion to the contact lens surface. For both lens types, lysozyme increased corneal cell adhesion to the contact lens, with the apparent cell monolayer relaxation modulus increasing up to an order of magnitude in the presence of Protein. The magnitude of this increase depended on the identity of the soaking solution: lenses soaked in borate-buffered solutions (BBS, Sensitive Eyes) exhibited a much greater increase in cell attachment upon Protein addition than those soaked in PBS. Significantly, all measurements were conducted while subjecting the cells to moderate surface pressures and shear rates, similar to those experienced by corneal cells in vivo.
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Corneal Cell Adhesion to Contact Lens Hydrogel Materials Enhanced via Tear Film Protein Deposition
2014Co-Authors: Claire M. Elkins, Gerald G FullerAbstract:Tear film Protein Deposition on contact lens hydrogels has been well characterized from the perspective of bacterial adhesion and viability. However, the effect of Protein Deposition on lens interactions with the corneal epithelium remains largely unexplored. The current study employs a live cell rheometer to quantify human corneal epithelial cell adhesion to soft contact lenses fouled with the tear film Protein lysozyme. PureVision balafilcon A and AirOptix lotrafilcon B lenses were soaked for five days in either phosphate buffered saline (PBS), borate buffered saline (BBS), or Sensitive Eyes Plus Saline Solution (Sensitive Eyes), either pure or in the presence of lysozyme. Treated contact lenses were then contacted to a live monolayer of corneal epithelial cells for two hours, after which the contact lens was sheared laterally. The apparent cell monolayer relaxation modulus was then used to quantify the extent of cell adhesion to the contact lens surface. For both lens types, lysozyme increased corneal cell adhesion to the contact lens, with the apparent cell monolayer relaxation modulus increasing up to an order of magnitude in the presence of Protein. The magnitude of this increase depended on the identity of the soaking solution: lenses soaked in borate-buffered solutions (BBS, Sensitive Eyes) exhibited a much greater increase in cell attachment upon Protein addition than those soaked in PBS. Significantly, all measurements were conducted while subjecting the cells to moderate surface pressures and shear rates, similar to those experienced by cornea
Dominique P Bureau - One of the best experts on this subject based on the ideXlab platform.
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estimating changes in essential amino acid requirements of rainbow trout and atlantic salmon as a function of body weight or diet composition using a novel factorial requirement model
Aquaculture, 2019Co-Authors: Katheline Hua, Dominique P BureauAbstract:Abstract As fish feeds are increasingly being formulated with a variety of economical Protein sources, more attention needs to be paid to ensure that the essential amino acids (EAAs) requirements of the fish are met adequately. A novel factorial AA requirement model was developed by incorporating the effect of dietary and biological factors on AA requirement and utilization for two salmonid fish species, Atlantic salmon and rainbow trout. The factorial model determines AA requirement of fish based on species, expected body weight gain, body Protein Deposition, AA profile in body Protein Deposition, maintenance AA requirement, and inevitable AA metabolism. Simulation using this novel model suggests that EAA requirement (expressed as % diet DM) progressively decreases when the live weight of the fish increases. Atlantic salmon appears to have higher EAA requirements (estimated as % diet DM) than rainbow trout at the same body weight classes, and the rate of decrease in EAA requirement with the increase of body weight appears to be slower in Atlantic salmon. Model simulations also suggest that the composition of the diet composition also influences the estimates of EAA requirements. The novel factorial model could be a helpful tool in formulating cost-effective aquaculture feeds. This modelling approach can be adapted to other fish species.
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diet digestible energy content affects lysine utilization but not dietary lysine requirements of rainbow trout oncorhynchus mykiss for maximum growth
Aquaculture, 2004Co-Authors: Pedro Encarnacao, M Rodehutscord, Cornelis F M De Lange, Dirk Hoehler, Wafa Bureau, Dominique P BureauAbstract:The effect of diet composition on essential amino acid (EAA) utilization and requirements is a topic of some controversy in fish. A growth trial was conducted to examine the effect of diet digestible energy (DE) content on lysine utilization and lysine requirement in rainbow trout (Oncorhynchus mykiss). Twelve isoproteic (40% digestible Protein (DP)) practical wheat-gluten-based diets, with two DE levels (16 and 20 MJ/kg) and six lysine levels (1.2% to 2.5%), were hand fed to near-satiety to triplicate groups of rainbow trout (initial weight 24 g) over 12 weeks. Across dietary lysine levels, higher (P 0.10) of diet DE content on whole body Protein Deposition. Based on body weight gain, diet DE content did not affect (P>0.10) lysine requirements, expressed as dietary concentration, while different statistical models yielded different (P<0.05) estimates of lysine requirements (% in diet, low vs. high diet DE content): broken line regression, 1.74 vs. 1.86; exponential, 2.28 vs. 2.33; asymptotic, 2.29 vs. 2.13. These results indicate that expressing lysine requirements in relation to DE content of the diet is not appropriate. At marginally deficient lysine intake levels, efficiency of lysine utilization for whole body Protein Deposition was affected both by lysine and DE levels in the diet. Effect of diet DE content on lysine utilization was also confirmed through liver glutamate dehydrogenase (GDH) activity. The effect of diet DE content on essential AA utilization of rainbow trout appears to be different from that seen in pigs and poultry.
Robert L Mauck - One of the best experts on this subject based on the ideXlab platform.
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Local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell–extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies—proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels—mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair. The extracellular matrix surrounding cells plays a significant role in their behaviour. The spreading, mechanosensing and differentiation of mesenchymal stem cells are shown to be dependent on the early Deposition and remodelling of local nascent Proteins within degradable and viscoelastic hydrogels.
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local nascent Protein Deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three dimensional hydrogels
Nature Materials, 2019Co-Authors: Claudia Loebel, Robert L Mauck, Jason A BurdickAbstract:Hydrogels serve as valuable tools for studying cell-extracellular matrix interactions in three-dimensional environments that recapitulate aspects of native extracellular matrix. However, the impact of early Protein Deposition on cell behaviour within hydrogels has largely been overlooked. Using a bio-orthogonal labelling technique, we visualized nascent Proteins within a day of culture across a range of hydrogels. In two engineered hydrogels of interest in three-dimensional mechanobiology studies-proteolytically degradable covalently crosslinked hyaluronic acid and dynamic viscoelastic hyaluronic acid hydrogels-mesenchymal stromal cell spreading, YAP/TAZ nuclear translocation and osteogenic differentiation were observed with culture. However, inhibition of cellular adhesion to nascent Proteins or reduction in nascent Protein remodelling reduced mesenchymal stromal cell spreading and nuclear translocation of YAP/TAZ, resulting in a shift towards adipogenic differentiation. Our findings emphasize the role of nascent Proteins in the cellular perception of engineered materials and have implications for in vitro cell signalling studies and application to tissue repair.