The Experts below are selected from a list of 14595 Experts worldwide ranked by ideXlab platform
Eiichi Momotani - One of the best experts on this subject based on the ideXlab platform.
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neutralization of interleukin 10 significantly enhances gamma interferon expression in peripheral blood by stimulation with johnin purified Protein Derivative and by infection with mycobacterium avium subsp paratuberculosis in experimentally infected
Infection and Immunity, 2004Co-Authors: Joram Josephat Buza, Hirokazu Hikono, Yasuyuki Mori, Reiko Nagata, Sachiyo Hirayama, Abusaleh M Bari, Yujing Shu, Noriko M Tsuji, Eiichi MomotaniAbstract:Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified Protein Derivative-induced whole-blood gamma interferon (IFN-γ) secretion 23-fold and also increased IFN-γ secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.
Alexander A Bankier - One of the best experts on this subject based on the ideXlab platform.
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tuberculosis value of lateral chest radiography in pre employment screening of patients with positive purified Protein Derivative skin test results
Radiology, 2009Co-Authors: Ronald L Eisenberg, Janneth Romero, Diana Litmanovich, Phillip M. Boiselle, Alexander A BankierAbstract:In a pre-employment setting, acquisition of one posteroanterior radiograph is sufficient for tuberculosis screening of individuals with abnormal purified Protein Derivative skin test results and will substantially reduce radiation exposure in this population.
Steven G. Reed - One of the best experts on this subject based on the ideXlab platform.
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The ID93 tuberculosis vaccine candidate does not induce sensitivity to purified Protein Derivative.
Clinical and Vaccine Immunology, 2014Co-Authors: Susan L. Baldwin, Valerie A. Reese, Brian Granger, Mark T. Orr, Gregory C. Ireton, Rhea N. Coler, Steven G. ReedAbstract:The tuberculin skin test (TST) is a simple and inexpensive test to determine whether individuals have been exposed to Mycobacterium tuberculosis. This test is not always reliable, however, in people previously immunized with BCG and/or who have been exposed to environmental mycobacterial species due to a reaction to purified Protein Derivative (PPD) used in the skin test. An issue with BCG, therefore, is that the resulting sensitization to PPD in some individuals compromises the diagnostic use of the skin test. The ability to induce protective immune responses without sensitizing to the tuberculin skin test will be important properties of next-generation tuberculosis (TB) vaccine candidates. We show here that guinea pigs immunized with the candidate TB vaccine ID93/GLA-SE, currently in clinical trials, do not react to intradermal PPD administration. In contrast, positive DTH responses to both ID93 and components thereof were induced in ID93/GLA-SE-immunized animals, indicating robust but specific cellular responses were present in the immunized animals. Noninterference with the TST is an important factor for consideration in the development of a vaccine against M. tuberculosis.
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human purified Protein Derivative specific cd4 t cells use both cd95 dependent and cd95 independent cytolytic mechanisms
Journal of Immunology, 1998Co-Authors: David M Lewinsohn, Steven G. Reed, Teresa Bement, David H Lynch, Kenneth H Grabstein, Mark R AldersonAbstract:CTL, both CD4+ and CD8+, are essential in the eradication of intracellular pathogens. Data generated using murine T cells have suggested a critical role for CD95 (Fas, Apo-1) in CD4+ T cell-induced apoptosis of target cells. In contrast, CD8+ CTL predominantly use the perforin/granzyme lytic pathway. At present little is known about the mechanism of CD4+ CTL lytic function during intracellular infection in humans. We have used human CD4+ T cells specific for purified Protein Derivative (PPD) of Mycobacterium tuberculosis to explore whether CD95 is the dominant cytolytic mechanism. PPD-reactive CD4+ clones efficiently lysed Ag-pulsed autologous monocytes, adherent macrophages, and EBV-transformed B cells. Addition of an antagonistic CD95 Ab had a minimal effect on cytolysis, whereas addition of MgEGTA to block perforin/granzyme resulted in complete inhibition of killing. In contrast, lysis of activated peripheral blood B cells could be partially blocked with the antagonistic CD95 Ab. Supporting these observations, monocytes, macrophages, and EBV-transformed B cells were not lysed by an agonistic CD95 Ab. Activated B cells were readily lysed by the agonistic CD95 Ab. T cell clones triggered through the TCR with anti-CD3 were capable of lysing the CD95-sensitive Jurkat T cell line in a CD95-dependent manner, but were also able to release granzymes. We conclude that human CD4+ T cells are capable of lysing PPD-pulsed targets using both perforin/granzyme and CD95 pathways. The contribution of CD95 is strictly dependent on target cell susceptibility to CD95-mediated killing.
Hasan Yazici - One of the best experts on this subject based on the ideXlab platform.
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infliximab does not suppress the tuberculin skin test purified Protein Derivative
The Journal of Rheumatology, 2007Co-Authors: Gulen Hatemi, Melike Melikoglu, Izzet Fresko, Seval Masatlioglu, Koray Tascilar, Hasan YaziciAbstract:OBJECTIVE: Tuberculin skin testing with purified Protein Derivative (PPD) is part of tuberculosis (TB) screening in patients receiving infliximab. We assessed whether infliximab, a strong inhibitor of inflammation, suppressed dermal induration, the outcome of this test. We also reassessed the booster phenomenon and the interobserver variability in tuberculin testing. METHODS: Forty-seven patients with various diagnoses, who had had a PPD test before infliximab use, were retested after infliximab treatment. The test was also assessed cross-sectionally among 31 patients with rheumatoid arthritis (RA) after 8.6 [+/- 4.1 standard deviation (SD)] months of infliximab use and in 82 patients with RA who had never used this agent. Booster phenomenon and the interobserver variability of reading the test were reassessed among 163 infliximab-naive patients with RA and Behcet's disease (BD) and 47 healthy controls. RESULTS: Among the 47 patients who received infliximab, and for whom sequential data were available, the mean skin induration was 5.9 +/- 8.0 SD mm before and 6.1 +/- 7.5 mm after 4.8 +/- 3.7 months of treatment (p = 0.890). In the cross-sectional study the mean PPD induration was 7.8 +/- 8.4 mm among infliximab-naive patients with RA, while it was 6.6 +/- 2.1 mm in those receiving infliximab (p = 0.271). Booster phenomenon was observed in 14/49 (29%) of patients with RA, 7/31 (23%) of those with BD, and 1/10 of healthy controls. Interobserver variability of PPD reading was good (kappa = 0.92). CONCLUSION: Infliximab use does not suppress the skin reaction to tuberculin. We confirm the booster phenomenon and that the PPD skin test has an acceptable interobserver reliability for an in vivo test.
Joram Josephat Buza - One of the best experts on this subject based on the ideXlab platform.
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neutralization of interleukin 10 significantly enhances gamma interferon expression in peripheral blood by stimulation with johnin purified Protein Derivative and by infection with mycobacterium avium subsp paratuberculosis in experimentally infected
Infection and Immunity, 2004Co-Authors: Joram Josephat Buza, Hirokazu Hikono, Yasuyuki Mori, Reiko Nagata, Sachiyo Hirayama, Abusaleh M Bari, Yujing Shu, Noriko M Tsuji, Eiichi MomotaniAbstract:Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified Protein Derivative-induced whole-blood gamma interferon (IFN-γ) secretion 23-fold and also increased IFN-γ secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.
