The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Beatrice Descampslatscha - One of the best experts on this subject based on the ideXlab platform.
-
advanced oxidation Protein Products as risk factors for atherosclerotic cardiovascular events in nondiabetic predialysis patients
American Journal of Kidney Diseases, 2005Co-Authors: Beatrice Descampslatscha, Veronique Witkosarsat, Valerie Gausson, Thao Nguyenkhoa, Anh Thu Nguyen, Nadya Mothu, Gerard M London, Paul JungersAbstract:Background: Inflammation and oxidative stress are established risk factors for atherosclerosis, but whether they contribute to the accelerated atherogenesis associated with chronic kidney disease (CKD) remains to be assessed at the predialysis stage. Methods: We prospectively examined the relationship between plasma levels of C-reactive Protein (CRP), fibrinogen, and advanced oxidation Protein Products (AOPPs), as selected markers of inflammation and oxidative stress, and incident first occlusive atherosclerotic cardiovascular (CV) events (ASCVEs) in a single-center cohort of 80 uremic predialysis patients without diabetes with a creatinine clearance ranging from 20 to 40 mL/min/1.73 m2. Results: During follow-up (median, 7 years), 21 patients developed coronary, cerebral, or peripheral artery occlusive accidents, an incidence of 44/1,000 patient-years. Except for older age, their conventional risk factors did not differ compared with the 59 patients who remained free of such accidents. Conversely, plasma levels of CRP (4.3 ± 2.7 versus 2.3 ± 2 mg/L; P = 0.005), fibrinogen (5.6 ± 1.4 versus 4.4 ± 1.2 mg/L; P = 0.0009), and AOPPs (58 ± 20 versus 42 ± 14 μmol/L; P = 0.0002) were significantly greater at baseline, although serum creatinine levels did not differ between the 2 groups. By multivariate Cox regression analysis, age and CRP, fibrinogen, and AOPP levels were significant independent predictors of ASCVEs. Risk factor–adjusted hazard ratios were as follows: age, 1.13 (95% confidence interval, 1.04 to 1.22; P = 0.002); CRP level, 1.37 (95% confidence interval, 1.05 to 1.79; P = 0.02); fibrinogen level, 2.23 (95% confidence interval, 1.20 to 4.13; P = 0.011); and AOPP level, 1.68 (95% confidence interval, 1.12 to 2.51; P = 0.011). Conclusion: CRP, fibrinogen, and AOPP levels independently predict ASCVEs in patients with CKD in the predialysis phase and might directly contribute to the uremia-associated accelerated atherogenesis.
-
biochemical and spectrophotometric significance of advanced oxidized Protein Products
Biochimica et Biophysica Acta, 2004Co-Authors: Chantal Capeillereblandin, Valerie Gausson, Beatrice Descampslatscha, Veronique WitkosarsatAbstract:We previously described the presence of advanced oxidation Protein Products (AOPP), a novel marker of oxidative stress in the plasma of hemodialyzed patients (HD). The present study was carried out to further investigate how myeloperoxidase (MPO)-catalyzed reactions could contribute to AOPP generation in the plasma. First, patterns of plasma Protein oxidation obtained after in vitro incubation of control plasma with hypochlorous acid (HOCl) were compared to those from HD patients and control plasma. The use of various analytical techniques enabled localising and identifying the main oxidized Proteins with albumin (HSA) after Protein separation by size-exclusion chromatography and SDS-PAGE electrophoresis. The characterization of the oxidation level of the individual plasma Proteins in terms of carbonyl groups and 3-nitrotyrosine formations was performed by immunoblotting. Secondly, to highlight the significance of AOPP index monitored by spectrophotometry, spectra were established for plasma fractions from HD patients and compared to data for control plasma and HOCl-treated plasma. The corresponding absorbance difference spectra were matched with external standards such as dityrosine, nitrotyrosine and pentosidine and elaborated chromophoric probe models. Indeed, HSA was chlorinated by HOCl reagent or HOCl generated via the MPO/H(2)O(2)/Cl(-) system and was nitrated by tetranitromethane. Increased absorbances at the range of 340 nm were observed both with chlorinated and nitrated HSA. Finally, our results indicate that HOCl, and not NO(2)(*), generated via MPO activity, could represent one of the pathways for AOPP production in plasma Proteins exposed to activated phagocytes.
-
are advanced oxidation Protein Products potential uremic toxins
Kidney International, 2003Co-Authors: Veronique Witkosarsat, Valerie Gausson, Beatrice DescampslatschaAbstract:Are advanced oxidation Protein Products potential uremic toxins? Oxidative stress, defined as a disruption of the equilibrium between the generation of oxidants and the activity of anti-oxidant systems, plays a significant role in the development of the inflammatory syndrome associated with chronic renal failure and hemodialysis. In our recent work, the aim of which was to better characterize oxidative stress in dialysis patients, we described the presence of oxidized Protein Products, which we have termed advanced oxidation Protein Products (AOPP), in the plasma of dialysis patients and we proposed AOPP as new markers of oxidative stress and potential inflammatory mediators. AOPP represent an exquisite marker of phagocyte-derived oxidative stress, and their role in the pathophysiology of chronic renal failure and dialysis-related complications might be of great importance. Regarding the mechanisms of generation of AOPP, we pointed out the importance of myeloperoxidase and the subsequent generation of chlorinated oxidants, previously considered solely as microbicidal agents, in the formation of AOPP. Indeed, AOPP appear to act as true inflammatory mediators since they are able to trigger the oxidative burst and the synthesis of inflammatory cytokines in neutrophils, as well as in monocytes. Thus, it could be hypothesized that the AOPP, which arise from the reaction between chlorinated oxidants and plasma Proteins, constitute new uremic toxins with pro-inflammatory effects.
-
iron therapy advanced oxidation Protein Products and carotid artery intima media thickness in end stage renal disease
Circulation, 2002Co-Authors: Tilman B. Drüeke, Veronique Witkosarsat, Ziad Massy, Beatrice Descampslatscha, A P Guerin, Sylvain J Marchais, Valerie Gausson, G LondonAbstract:Background— Increased common carotid artery intima-media thickness (CCA-IMT) is a marker of early atherosclerosis. Low-grade inflammation is associated with the pathogenesis of atherosclerosis. Low-grade inflammation and increased CCA-IMT are observed in end-stage renal disease (ESRD). Oxidative stress is involved in uremia-related inflammation. Advanced oxidation Protein Products (AOPP) are markers of oxidant-mediated Protein damage in ESRD. Intravenous iron given to patients on hemodialysis (HD) might induce oxidative stress. We investigated the relationships between AOPP, iron therapy, and CCA-IMT in stable HD patients. Methods and Results— Plasma AOPP and blood chemistry, including iron status, were analyzed in a cohort of 79 ESRD patients on HD. Measurements of CCA-IMT and CCA diameter, as assessed by B-mode ultrasonography, were obtained in 60 patients. AOPP levels were elevated in ESRD patients, and in univariate (r=0.42, P<0.0001) and multivariate analyses (r=0.38, P<0.001), they correlated with s...
-
advanced oxidation Protein Products as novel mediators of inflammation and monocyte activation in chronic renal failure
Journal of Immunology, 1998Co-Authors: Veronique Witkosarsat, Tilman B. Drüeke, Chantal Capeillereblandin, Miriam A Friedlander, Anh Thu Nguyen, Thao Nguyen Khoa, Sandrine Canteloup, Jeanmichel Dayer, P Jungers, Beatrice DescampslatschaAbstract:We previously demonstrated the presence of advanced oxidation Protein Products (AOPP), a novel marker of oxidative stress in the plasma of uremic patients receiving maintenance dialysis. The present study in a cohort of 162 uremic patients showed that plasma concentrations of AOPP increased with progression of chronic renal failure and were closely related to advanced glycation end Products (AGE)-pentosidine (r = 0.52, p < 0.001), taken as a marker of AGE. In vivo, the relevance of AOPP and AGE-pentosidine in monocyte-mediated inflammatory syndrome associated with uremia was evidenced by close correlations between AOPP or AGE-pentosidine and monocyte activation markers, including neopterin, IL-1R antagonist, TNF-α, and TNF soluble receptors (TNF-sR55 and TNF-sR75). To determine the mechanisms by which AOPP and AGE could be directly involved in monocyte activation, AOPP-human serum albumin (HSA) and AGE-HSA were produced in vitro by treating HSA with oxidants or glucose, respectively. Spectroscopic analysis confirmed that AOPP-HSA contains carbonyls and dityrosine. Both AOPP-HSA and AGE-HSA, but not purified dityrosine, were capable of triggering the oxidative burst of human monocytes in cultures. The AOPP-HSA-induced respiratory burst was dependent on the chlorinated nature of the oxidant and on the molar ratio HSA/HOCl. Collectively, these data first demonstrate that AOPP act as a mediator of oxidative stress and monocyte respiratory burst, which points to monocytes as both target and actor in the immune dysregulation associated with chronic uremia.
Veronique Witkosarsat - One of the best experts on this subject based on the ideXlab platform.
-
advanced oxidation Protein Products as risk factors for atherosclerotic cardiovascular events in nondiabetic predialysis patients
American Journal of Kidney Diseases, 2005Co-Authors: Beatrice Descampslatscha, Veronique Witkosarsat, Valerie Gausson, Thao Nguyenkhoa, Anh Thu Nguyen, Nadya Mothu, Gerard M London, Paul JungersAbstract:Background: Inflammation and oxidative stress are established risk factors for atherosclerosis, but whether they contribute to the accelerated atherogenesis associated with chronic kidney disease (CKD) remains to be assessed at the predialysis stage. Methods: We prospectively examined the relationship between plasma levels of C-reactive Protein (CRP), fibrinogen, and advanced oxidation Protein Products (AOPPs), as selected markers of inflammation and oxidative stress, and incident first occlusive atherosclerotic cardiovascular (CV) events (ASCVEs) in a single-center cohort of 80 uremic predialysis patients without diabetes with a creatinine clearance ranging from 20 to 40 mL/min/1.73 m2. Results: During follow-up (median, 7 years), 21 patients developed coronary, cerebral, or peripheral artery occlusive accidents, an incidence of 44/1,000 patient-years. Except for older age, their conventional risk factors did not differ compared with the 59 patients who remained free of such accidents. Conversely, plasma levels of CRP (4.3 ± 2.7 versus 2.3 ± 2 mg/L; P = 0.005), fibrinogen (5.6 ± 1.4 versus 4.4 ± 1.2 mg/L; P = 0.0009), and AOPPs (58 ± 20 versus 42 ± 14 μmol/L; P = 0.0002) were significantly greater at baseline, although serum creatinine levels did not differ between the 2 groups. By multivariate Cox regression analysis, age and CRP, fibrinogen, and AOPP levels were significant independent predictors of ASCVEs. Risk factor–adjusted hazard ratios were as follows: age, 1.13 (95% confidence interval, 1.04 to 1.22; P = 0.002); CRP level, 1.37 (95% confidence interval, 1.05 to 1.79; P = 0.02); fibrinogen level, 2.23 (95% confidence interval, 1.20 to 4.13; P = 0.011); and AOPP level, 1.68 (95% confidence interval, 1.12 to 2.51; P = 0.011). Conclusion: CRP, fibrinogen, and AOPP levels independently predict ASCVEs in patients with CKD in the predialysis phase and might directly contribute to the uremia-associated accelerated atherogenesis.
-
biochemical and spectrophotometric significance of advanced oxidized Protein Products
Biochimica et Biophysica Acta, 2004Co-Authors: Chantal Capeillereblandin, Valerie Gausson, Beatrice Descampslatscha, Veronique WitkosarsatAbstract:We previously described the presence of advanced oxidation Protein Products (AOPP), a novel marker of oxidative stress in the plasma of hemodialyzed patients (HD). The present study was carried out to further investigate how myeloperoxidase (MPO)-catalyzed reactions could contribute to AOPP generation in the plasma. First, patterns of plasma Protein oxidation obtained after in vitro incubation of control plasma with hypochlorous acid (HOCl) were compared to those from HD patients and control plasma. The use of various analytical techniques enabled localising and identifying the main oxidized Proteins with albumin (HSA) after Protein separation by size-exclusion chromatography and SDS-PAGE electrophoresis. The characterization of the oxidation level of the individual plasma Proteins in terms of carbonyl groups and 3-nitrotyrosine formations was performed by immunoblotting. Secondly, to highlight the significance of AOPP index monitored by spectrophotometry, spectra were established for plasma fractions from HD patients and compared to data for control plasma and HOCl-treated plasma. The corresponding absorbance difference spectra were matched with external standards such as dityrosine, nitrotyrosine and pentosidine and elaborated chromophoric probe models. Indeed, HSA was chlorinated by HOCl reagent or HOCl generated via the MPO/H(2)O(2)/Cl(-) system and was nitrated by tetranitromethane. Increased absorbances at the range of 340 nm were observed both with chlorinated and nitrated HSA. Finally, our results indicate that HOCl, and not NO(2)(*), generated via MPO activity, could represent one of the pathways for AOPP production in plasma Proteins exposed to activated phagocytes.
-
are advanced oxidation Protein Products potential uremic toxins
Kidney International, 2003Co-Authors: Veronique Witkosarsat, Valerie Gausson, Beatrice DescampslatschaAbstract:Are advanced oxidation Protein Products potential uremic toxins? Oxidative stress, defined as a disruption of the equilibrium between the generation of oxidants and the activity of anti-oxidant systems, plays a significant role in the development of the inflammatory syndrome associated with chronic renal failure and hemodialysis. In our recent work, the aim of which was to better characterize oxidative stress in dialysis patients, we described the presence of oxidized Protein Products, which we have termed advanced oxidation Protein Products (AOPP), in the plasma of dialysis patients and we proposed AOPP as new markers of oxidative stress and potential inflammatory mediators. AOPP represent an exquisite marker of phagocyte-derived oxidative stress, and their role in the pathophysiology of chronic renal failure and dialysis-related complications might be of great importance. Regarding the mechanisms of generation of AOPP, we pointed out the importance of myeloperoxidase and the subsequent generation of chlorinated oxidants, previously considered solely as microbicidal agents, in the formation of AOPP. Indeed, AOPP appear to act as true inflammatory mediators since they are able to trigger the oxidative burst and the synthesis of inflammatory cytokines in neutrophils, as well as in monocytes. Thus, it could be hypothesized that the AOPP, which arise from the reaction between chlorinated oxidants and plasma Proteins, constitute new uremic toxins with pro-inflammatory effects.
-
iron therapy advanced oxidation Protein Products and carotid artery intima media thickness in end stage renal disease
Circulation, 2002Co-Authors: Tilman B. Drüeke, Veronique Witkosarsat, Ziad Massy, Beatrice Descampslatscha, A P Guerin, Sylvain J Marchais, Valerie Gausson, G LondonAbstract:Background— Increased common carotid artery intima-media thickness (CCA-IMT) is a marker of early atherosclerosis. Low-grade inflammation is associated with the pathogenesis of atherosclerosis. Low-grade inflammation and increased CCA-IMT are observed in end-stage renal disease (ESRD). Oxidative stress is involved in uremia-related inflammation. Advanced oxidation Protein Products (AOPP) are markers of oxidant-mediated Protein damage in ESRD. Intravenous iron given to patients on hemodialysis (HD) might induce oxidative stress. We investigated the relationships between AOPP, iron therapy, and CCA-IMT in stable HD patients. Methods and Results— Plasma AOPP and blood chemistry, including iron status, were analyzed in a cohort of 79 ESRD patients on HD. Measurements of CCA-IMT and CCA diameter, as assessed by B-mode ultrasonography, were obtained in 60 patients. AOPP levels were elevated in ESRD patients, and in univariate (r=0.42, P<0.0001) and multivariate analyses (r=0.38, P<0.001), they correlated with s...
-
advanced oxidation Protein Products as novel mediators of inflammation and monocyte activation in chronic renal failure
Journal of Immunology, 1998Co-Authors: Veronique Witkosarsat, Tilman B. Drüeke, Chantal Capeillereblandin, Miriam A Friedlander, Anh Thu Nguyen, Thao Nguyen Khoa, Sandrine Canteloup, Jeanmichel Dayer, P Jungers, Beatrice DescampslatschaAbstract:We previously demonstrated the presence of advanced oxidation Protein Products (AOPP), a novel marker of oxidative stress in the plasma of uremic patients receiving maintenance dialysis. The present study in a cohort of 162 uremic patients showed that plasma concentrations of AOPP increased with progression of chronic renal failure and were closely related to advanced glycation end Products (AGE)-pentosidine (r = 0.52, p < 0.001), taken as a marker of AGE. In vivo, the relevance of AOPP and AGE-pentosidine in monocyte-mediated inflammatory syndrome associated with uremia was evidenced by close correlations between AOPP or AGE-pentosidine and monocyte activation markers, including neopterin, IL-1R antagonist, TNF-α, and TNF soluble receptors (TNF-sR55 and TNF-sR75). To determine the mechanisms by which AOPP and AGE could be directly involved in monocyte activation, AOPP-human serum albumin (HSA) and AGE-HSA were produced in vitro by treating HSA with oxidants or glucose, respectively. Spectroscopic analysis confirmed that AOPP-HSA contains carbonyls and dityrosine. Both AOPP-HSA and AGE-HSA, but not purified dityrosine, were capable of triggering the oxidative burst of human monocytes in cultures. The AOPP-HSA-induced respiratory burst was dependent on the chlorinated nature of the oxidant and on the molar ratio HSA/HOCl. Collectively, these data first demonstrate that AOPP act as a mediator of oxidative stress and monocyte respiratory burst, which points to monocytes as both target and actor in the immune dysregulation associated with chronic uremia.
Amarinder Singh Bawa - One of the best experts on this subject based on the ideXlab platform.
-
Functional and edible uses of soy Protein Products
Comprehensive Reviews in Food Science and Food Safety, 2008Co-Authors: Preeti Singh, S. N. Sabapathy, R Kumar, Amarinder Singh BawaAbstract:ABSTRACT: Consumers are becoming increasingly interested in healthful foods and are open to soy Protein ingredients. Soybeans as food are very versatile and a rich source of essential nutrients. They are also an excellent source of good-quality Protein, comparable to other Protein foods, and suitable for all ages. Adverse nutritional and other undesirable effects followed by the consumption of raw soybean meal have been attributed to the presence of endogenous inhibitors of digestive enzymes and lectins, as well as poor digestibility. To improve the nutritional quality of soy foods, inhibitors and lectins are generally inactivated by heat or eliminated by fractionation during food processing. Soybeans provide an alternative source of Protein for people who are allergic to milk Protein. Soy Protein is highly digestible (92% to 100%) and contains all essential amino acids. Although relatively low in methionine, it is a good source of lysine. Soy-Protein Products contain a high concentration of isoflavones, up to 1 g/kg. Increased acceptance of soy Proteins is due to unmatched qualities like good functional properties in food applications, high nutritional quality, abundance, availability, and low cost. At present the various forms of soy Proteins are primarily utilized for their functional effects rather than their nutritional properties. This article summarizes the integrated overview of the widely available, scattered information about the nutritional and functional uses of the soy Proteins when applied in food systems and intends to present the most current knowledge with an interest to stimulate further research to optimize their beneficial effects.
R Kumar - One of the best experts on this subject based on the ideXlab platform.
-
Functional and edible uses of soy Protein Products
Comprehensive Reviews in Food Science and Food Safety, 2008Co-Authors: Preeti Singh, S. N. Sabapathy, R Kumar, Amarinder Singh BawaAbstract:ABSTRACT: Consumers are becoming increasingly interested in healthful foods and are open to soy Protein ingredients. Soybeans as food are very versatile and a rich source of essential nutrients. They are also an excellent source of good-quality Protein, comparable to other Protein foods, and suitable for all ages. Adverse nutritional and other undesirable effects followed by the consumption of raw soybean meal have been attributed to the presence of endogenous inhibitors of digestive enzymes and lectins, as well as poor digestibility. To improve the nutritional quality of soy foods, inhibitors and lectins are generally inactivated by heat or eliminated by fractionation during food processing. Soybeans provide an alternative source of Protein for people who are allergic to milk Protein. Soy Protein is highly digestible (92% to 100%) and contains all essential amino acids. Although relatively low in methionine, it is a good source of lysine. Soy-Protein Products contain a high concentration of isoflavones, up to 1 g/kg. Increased acceptance of soy Proteins is due to unmatched qualities like good functional properties in food applications, high nutritional quality, abundance, availability, and low cost. At present the various forms of soy Proteins are primarily utilized for their functional effects rather than their nutritional properties. This article summarizes the integrated overview of the widely available, scattered information about the nutritional and functional uses of the soy Proteins when applied in food systems and intends to present the most current knowledge with an interest to stimulate further research to optimize their beneficial effects.
-
adhesives and plastics based on soy Protein Products
Industrial Crops and Products, 2002Co-Authors: R Kumar, Veena Choudhary, Saroj Mishra, I K Varma, Bo MattiasonAbstract:Significance of eco-friendly materials based on easily renewable natural resources, and the finite nature of petrochemical resources, has necessitated the development of polymers from agricultural processing by Products such as soy Proteins from oil processing. Although, considerable work was done in the early part of last century on polymers based on soy Protein, there was almost no activity in this field for the last fifty years. There is a need to critically analyse the available literature on soy Protein based polymeric materials. Therefore, an attempt is made to review the state-of-the-art of the polymeric materials with emphasis on adhesives and plastics derived from soy Protein, a renewable resource abundantly available in nature.
Bo Mattiason - One of the best experts on this subject based on the ideXlab platform.
-
adhesives and plastics based on soy Protein Products
Industrial Crops and Products, 2002Co-Authors: R Kumar, Veena Choudhary, Saroj Mishra, I K Varma, Bo MattiasonAbstract:Significance of eco-friendly materials based on easily renewable natural resources, and the finite nature of petrochemical resources, has necessitated the development of polymers from agricultural processing by Products such as soy Proteins from oil processing. Although, considerable work was done in the early part of last century on polymers based on soy Protein, there was almost no activity in this field for the last fifty years. There is a need to critically analyse the available literature on soy Protein based polymeric materials. Therefore, an attempt is made to review the state-of-the-art of the polymeric materials with emphasis on adhesives and plastics derived from soy Protein, a renewable resource abundantly available in nature.
