The Experts below are selected from a list of 2649 Experts worldwide ranked by ideXlab platform
Edward F. Domino - One of the best experts on this subject based on the ideXlab platform.
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Zinc Protoporphyrin, Zinc Ion, and Protoporphyrin Reduce Focal Cerebral Ischemia
Stroke, 1996Co-Authors: Yong Jie Zhao, Guo-yuan Yang, Edward F. DominoAbstract:Background and Purpose Zinc Protoporphyrin pretreatment protects against temporary focal ischemic brain injury in rats. However, it is not known whether the Zinc or the Protoporphyrin portion of Zinc Protoporphyrin has effects on focal cerebral ischemia. Hence, all three agents were compared with regard to infarct size and edema in a rat model of middle cerebral artery occlusion. Methods Four groups of adult male Sprague-Dawley rats were subjected to 2 hours of temporary middle cerebral artery occlusion followed by 22 hours of reperfusion. Each group was pretreated 30 minutes before middle cerebral artery occlusion with 0.9% NaCl, and then three groups were given equimolar doses of Zinc Protoporphyrin, Zinc chloride, or Protoporphyrin, respectively. Regional cerebral blood flow in the ischemic cortex was monitored with a laser Doppler flowmeter. Cerebral infarct size, brain water content, and ion content were measured 24 hours after the onset of occlusion. Results Regional cerebral blood flow during middl...
Yong Jie Zhao - One of the best experts on this subject based on the ideXlab platform.
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Zinc Protoporphyrin, Zinc Ion, and Protoporphyrin Reduce Focal Cerebral Ischemia
Stroke, 1996Co-Authors: Yong Jie Zhao, Guo-yuan Yang, Edward F. DominoAbstract:Background and Purpose Zinc Protoporphyrin pretreatment protects against temporary focal ischemic brain injury in rats. However, it is not known whether the Zinc or the Protoporphyrin portion of Zinc Protoporphyrin has effects on focal cerebral ischemia. Hence, all three agents were compared with regard to infarct size and edema in a rat model of middle cerebral artery occlusion. Methods Four groups of adult male Sprague-Dawley rats were subjected to 2 hours of temporary middle cerebral artery occlusion followed by 22 hours of reperfusion. Each group was pretreated 30 minutes before middle cerebral artery occlusion with 0.9% NaCl, and then three groups were given equimolar doses of Zinc Protoporphyrin, Zinc chloride, or Protoporphyrin, respectively. Regional cerebral blood flow in the ischemic cortex was monitored with a laser Doppler flowmeter. Cerebral infarct size, brain water content, and ion content were measured 24 hours after the onset of occlusion. Results Regional cerebral blood flow during middl...
Guo-yuan Yang - One of the best experts on this subject based on the ideXlab platform.
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Zinc Protoporphyrin, Zinc Ion, and Protoporphyrin Reduce Focal Cerebral Ischemia
Stroke, 1996Co-Authors: Yong Jie Zhao, Guo-yuan Yang, Edward F. DominoAbstract:Background and Purpose Zinc Protoporphyrin pretreatment protects against temporary focal ischemic brain injury in rats. However, it is not known whether the Zinc or the Protoporphyrin portion of Zinc Protoporphyrin has effects on focal cerebral ischemia. Hence, all three agents were compared with regard to infarct size and edema in a rat model of middle cerebral artery occlusion. Methods Four groups of adult male Sprague-Dawley rats were subjected to 2 hours of temporary middle cerebral artery occlusion followed by 22 hours of reperfusion. Each group was pretreated 30 minutes before middle cerebral artery occlusion with 0.9% NaCl, and then three groups were given equimolar doses of Zinc Protoporphyrin, Zinc chloride, or Protoporphyrin, respectively. Regional cerebral blood flow in the ischemic cortex was monitored with a laser Doppler flowmeter. Cerebral infarct size, brain water content, and ion content were measured 24 hours after the onset of occlusion. Results Regional cerebral blood flow during middl...
Patrick E. Mckinney - One of the best experts on this subject based on the ideXlab platform.
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Acute elevation of blood lead levels within hours of ingestion of large quantities of lead shot.
Journal of toxicology. Clinical toxicology, 2000Co-Authors: Patrick E. MckinneyAbstract:Background: Ingestion of elemental lead foreign bodies is felt to have a low risk of clinically significant lead absorption unless gastrointestinal pathology and/or prolonged transit time are present. We present a case of ingestion of a large quantity of small diameter lead shot accompanied by rapid elevation of blood lead levels. Case Report: A 5½-year-old previously healthy girl was found eating the pellets from an ankle weight. She vomited and complained of abdominal pain. In the emergency department, she had no complaints and normal vital signs. An abdominal X-ray showed thousands of small, round, metallic density objects in the stomach. Her white blood cell count was 14,700/mm3, and the hemoglobin, mean corpuscular volume, free erythrocyte Protoporphyrin, Zinc Protoporphyrin, biochemistry panel 21, and urinalysis were normal. She had no prior lead level for comparison. Whole-bowel irrigation was begun and she passed over 11 stools with pellets as well as other foreign bodies (erasers, bead, etc.) in ...
Hermann Berg - One of the best experts on this subject based on the ideXlab platform.
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Membrane electroporation increases photodynamic effects
Journal of Electroanalytical Chemistry, 2000Co-Authors: Aihua Zhou, Man Liu, Cristina Baciu, Brigitte Glück, Hermann BergAbstract:Abstract Electroporation of membranes is used widely for drug delivery. Photodynamic action consists of three main steps: (A) incorporation of the sensitizer through a membrane into cells; (B) photoxidation of cell constituents and (C) reoxidation of the reduced sensitizer by oxygen etc. The mechanisms of (B) and (C) have been studied widely in past decades. However, the mechanism of transport (A) of sensitizers to targets as the rate limiting step has not been studied to the same extent. Therefore we applied membrane and cell wall electroporation of human histiocytic lymphoma U937 and Saccharomyces cerevisiae cells in order to incorporate rapidly the reliable photodynamic agents thiopyronine, Protoporphyrin, Zinc phthalocyanine, copper phthalocyanine sulfonate, adriamycin and daunomysin, well-tried cytostatic agents. Depending on field strength and pulse width, 50–90% of cells become electroporated, then the dye diffuses rapidly into the cells, which reseal their membranes over a period of 6–10 min. Illumination for 10–15 min destroys all resealed cells faster than the same amount of unporated cells as in the case of the control (without pulse treatment) either by oxidation of cell components caused by excited dyes or singlet oxygen treatment. By this synergism of electroporation and photodynamic action at the same time, it is possible to kill all cells in a much shorter time than under usual conditions, e.g. in the control suspension. A combination of electrochemotherapeutic needle electrodes with a light conductor for a LASER connection will be effective for therapy.
