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Josep Guarro - One of the best experts on this subject based on the ideXlab platform.
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2010. Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp
2016Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseu-dallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has dem-onstrated that they are different species. Since a teleomorph was not observed among isolates recently identifi ed as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described. Keywords Heterothallism, Scedosporium spp. Pseudallescheria spp. Until recently, it was thought that S. apiospermum was its anamorph [ 5,6], but based on a multilocus sequence analysis, Gilgado et al. [ 2] delineated numerous phyloge-netic species among P. boydii or S. apiospermum isolates
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Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp. nov.
Medical mycology, 2010Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseudallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has demonstrated that they are different species. Since a teleomorph was not observed among isolates recently identified as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described.
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molecular and phenotypic data supporting distinct species statuses for scedosporium apiospermum and Pseudallescheria boydii and the proposed new species scedosporium dehoogii
Journal of Clinical Microbiology, 2008Co-Authors: Felix Gilgado, Josep Cano, Deanna A. Sutton, Josepa Gené, Josep GuarroAbstract:Based on the morphological, physiologic, and molecular (β-tubulin gene) study of 141 isolates of the Pseudallescheria boydii species complex (including several synonyms) and relatives, the new species Scedosporium dehoogii is proposed. Scedosporium apiospermum and P. boydii are considered two different species and the new name Scedosporium boydii is proposed for the anamorph of the latter species. A summary of the key morphological and physiological features for distinguishing the species of Pseudallescheria/Scedosporium is provided.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. β-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84T, CBS 311.72T and CBS 129.78T.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. beta-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84(T), CBS 311.72(T) and CBS 129.78(T).
Felix Gilgado - One of the best experts on this subject based on the ideXlab platform.
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2010. Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp
2016Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseu-dallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has dem-onstrated that they are different species. Since a teleomorph was not observed among isolates recently identifi ed as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described. Keywords Heterothallism, Scedosporium spp. Pseudallescheria spp. Until recently, it was thought that S. apiospermum was its anamorph [ 5,6], but based on a multilocus sequence analysis, Gilgado et al. [ 2] delineated numerous phyloge-netic species among P. boydii or S. apiospermum isolates
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abundance of Pseudallescheria scedosporium species in the australian urban environment suggests a possible source for scedosporiosis including the colonization of airways in cystic fibrosis
Medical Mycology, 2010Co-Authors: Felix Gilgado, Azian Harun, Sharon C.-a. Chen, Wieland MeyerAbstract:Members of the Pseudallescheria/Scedosporium species complex are emerging opportunistic fungal pathogens which have the capacity to colonize patients with damaged airways, including those with cystic fibrosis (CF). Assuming human infection is acquired via inhalation of fungal spores from the environment, we performed a qualitative environmental survey encompassing 25 urban, semirural and rural sites in the greater Sydney region to determine the prevalence of Pseudallescheria/Scedosporium species. Soil sampling revealed an abundance of Pseudallescheria/Scedosporium, particularly in locations associated with high human activity. No variation was noted during repeated sampling at different times of the year. Strains of Scedosporium aurantiacum were most frequently isolated (54.6%), followed by Scedosporium prolificans (43%), P. boydii (2.1%) and S. dehoogii (0.3%). The findings coincide with the relatively high prevalence of Scedosporium infections in Australia and their presence as colonizers in CF patients. They emphasize the importance of environmental studies to assess the clinical risk of infection.
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Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp. nov.
Medical mycology, 2010Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseudallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has demonstrated that they are different species. Since a teleomorph was not observed among isolates recently identified as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described.
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Abundance of Pseudallescheria/Scedosporium species in the Australian urban environment suggests a possible source for scedosporiosis including the colonization of airways in cystic fibrosis
Medical Mycology, 2010Co-Authors: Azian Harun, Felix Gilgado, Sharon C.-a. Chen, Wieland MeyerAbstract:Members of the Pseudallescheria/Scedosporium species complex are emerging opportunistic fungal pathogens which have the capacity to colonize patients with damaged airways, including those with cystic fibrosis (CF). Assuming human infection is acquired via inhalation of fungal spores from the environment, we performed a qualitative environmental survey encompassing 25 urban, semirural and rural sites in the greater Sydney region to determine the prevalence of Pseudallescheria/Scedosporium species. Soil sampling revealed an abundance of Pseudallescheria/Scedosporium, particularly in locations associated with high human activity. No variation was noted during repeated sampling at different times of the year. Strains of Scedosporium aurantiacum were most frequently isolated (54.6%), followed by Scedosporium prolificans (43%), P. boydii (2.1%) and S. dehoogii (0.3%). The findings coincide with the relatively high prevalence of Scedosporium infections in Australia and their presence as colonizers in CF patients. They emphasize the importance of environmental studies to assess the clinical risk of infection.
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molecular and phenotypic data supporting distinct species statuses for scedosporium apiospermum and Pseudallescheria boydii and the proposed new species scedosporium dehoogii
Journal of Clinical Microbiology, 2008Co-Authors: Felix Gilgado, Josep Cano, Deanna A. Sutton, Josepa Gené, Josep GuarroAbstract:Based on the morphological, physiologic, and molecular (β-tubulin gene) study of 141 isolates of the Pseudallescheria boydii species complex (including several synonyms) and relatives, the new species Scedosporium dehoogii is proposed. Scedosporium apiospermum and P. boydii are considered two different species and the new name Scedosporium boydii is proposed for the anamorph of the latter species. A summary of the key morphological and physiological features for distinguishing the species of Pseudallescheria/Scedosporium is provided.
G S De Hoog - One of the best experts on this subject based on the ideXlab platform.
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Susceptibility and diversity in the therapy-refractory genus scedosporium
'American Society for Microbiology', 2014Co-Authors: Lackner M., Hagen F., Meis J.f.g.m., Gerrits-van Den Ende A.h., Vu D., Fritz J., Moussa T.a., G S De HoogAbstract:Contains fulltext : 136891.pdf (publisher's version ) (Open Access)Scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. Acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. Inter- and intraspecific diversities of Scedosporium species were analyzed for three partitions (rDNA internal transcribed spacer gene [ITS], partial beta-tubulin gene, and amplified fragment length polymorphism profiles), with the aim to establish distribution of resistance between species, populations, and strains. Heterogeneity of and recombination between lineages were determined, and distances between clusters were calculated using a centroid approach. Clinical, geographic, and antifungal data were plotted on diversity networks. Scedosporium minutisporum, Scedosporium desertorum, and Scedosporium aurantiacum were distinguished unambiguously in all partitions and had differential antifungal susceptibility profiles (ASP). Pseudallescheria fusoidea and Pseudallescheria ellipsoidea were indistinguishable from Scedosporium boydii. Pseudallescheria angusta took an intermediate position between Scedosporium apiospermum and S. boydii. Scedosporium boydii and S. apiospermum had identical ASP. Differences in (multi)resistance were linked to individual strains. S. apiospermum and S. boydii showed limited interbreeding and were recognized as valid, sympatric species. The S. apiospermum/S. boydii group, comprising the main clinically relevant Scedosporium species, consists of separate lineages and is interpreted as a complex undergoing sympatric evolution with incomplete lineage sorting. In routine diagnostics, the lineages in S. apiospermum/S. boydii are indicated with the umbrella descriptor "S. apiospermum complex"; individual species can be identified with rDNA ITS with 96.3% confidence. Voriconazole is recommended as the first-line treatment; resistance against this compound is rare
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Susceptibility and diversity in the therapy-refractory genus scedosporium
2014Co-Authors: Lackner M., Hagen F., Meis J.f.g.m., Gerrits-van Den Ende A.h., Vu D., Fritz J., Moussa T.a., G S De HoogAbstract:Scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. Acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. Inter- and intraspecific diversities of Scedosporium species were analyzed for three partitions (rDNA internal transcribed spacer gene [ITS], partial beta-tubulin gene, and amplified fragment length polymorphism profiles), with the aim to establish distribution of resistance between species, populations, and strains. Heterogeneity of and recombination between lineages were determined, and distances between clusters were calculated using a centroid approach. Clinical, geographic, and antifungal data were plotted on diversity networks. Scedosporium minutisporum, Scedosporium desertorum, and Scedosporium aurantiacum were distinguished unambiguously in all partitions and had differential antifungal susceptibility profiles (ASP). Pseudallescheria fusoidea and Pseudallescheria ellipsoidea were indistinguishable from Scedosporium boydii. Pseudallescheria angusta took an intermediate position between Scedosporium apiospermum and S. boydii. Scedosporium boydii and S. apiospermum had identical ASP. Differences in (multi)resistance were linked to individual strains. S. apiospermum and S. boydii showed limited interbreeding and were recognized as valid, sympatric species. The S. apiospermum/S. boydii group, comprising the main clinically relevant Scedosporium species, consists of separate lineages and is interpreted as a complex undergoing sympatric evolution with incomplete lineage sorting. In routine diagnostics, the lineages in S. apiospermum/S. boydii are indicated with the umbrella descriptor "S. apiospermum complex"; individual species can be identified with rDNA ITS with 96.3% confidence. Voriconazole is recommended as the first-line treatment; resistance against this compound is rare
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Molecular identification tools for sibling species of Scedosporium and Pseudallescheria
Medical mycology, 2011Co-Authors: Michaela Lackner, A H G Gerrits Van Den Ende, Corné H. W. Klaassen, Jacques F. Meis, G S De HoogAbstract:The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 s-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii.
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reverse line blot hybridisation screening of Pseudallescheria scedosporium species in patients with cystic fibrosis
Mycoses, 2011Co-Authors: A Gerrits H G Van Den Ende, Jean-philippe Bouchara, G S De Hoog, I Accoceberry, F Hernandez, Isabelle Durandjoly, L DelhaesAbstract:The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.
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Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis.
Mycoses, 2011Co-Authors: A H G Gerrits Van Den Ende, G S De Hoog, I Accoceberry, I Durand-joly, J-p Bouchara, F Hernandez, L DelhaesAbstract:The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.
Josepa Gené - One of the best experts on this subject based on the ideXlab platform.
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2010. Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp
2016Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseu-dallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has dem-onstrated that they are different species. Since a teleomorph was not observed among isolates recently identifi ed as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described. Keywords Heterothallism, Scedosporium spp. Pseudallescheria spp. Until recently, it was thought that S. apiospermum was its anamorph [ 5,6], but based on a multilocus sequence analysis, Gilgado et al. [ 2] delineated numerous phyloge-netic species among P. boydii or S. apiospermum isolates
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Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp. nov.
Medical mycology, 2010Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseudallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has demonstrated that they are different species. Since a teleomorph was not observed among isolates recently identified as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described.
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molecular and phenotypic data supporting distinct species statuses for scedosporium apiospermum and Pseudallescheria boydii and the proposed new species scedosporium dehoogii
Journal of Clinical Microbiology, 2008Co-Authors: Felix Gilgado, Josep Cano, Deanna A. Sutton, Josepa Gené, Josep GuarroAbstract:Based on the morphological, physiologic, and molecular (β-tubulin gene) study of 141 isolates of the Pseudallescheria boydii species complex (including several synonyms) and relatives, the new species Scedosporium dehoogii is proposed. Scedosporium apiospermum and P. boydii are considered two different species and the new name Scedosporium boydii is proposed for the anamorph of the latter species. A summary of the key morphological and physiological features for distinguishing the species of Pseudallescheria/Scedosporium is provided.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. β-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84T, CBS 311.72T and CBS 129.78T.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. beta-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84(T), CBS 311.72(T) and CBS 129.78(T).
Josep Cano - One of the best experts on this subject based on the ideXlab platform.
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2010. Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp
2016Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseu-dallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has dem-onstrated that they are different species. Since a teleomorph was not observed among isolates recently identifi ed as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described. Keywords Heterothallism, Scedosporium spp. Pseudallescheria spp. Until recently, it was thought that S. apiospermum was its anamorph [ 5,6], but based on a multilocus sequence analysis, Gilgado et al. [ 2] delineated numerous phyloge-netic species among P. boydii or S. apiospermum isolates
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Heterothallism in Scedosporium apiospermum and description of its teleomorph Pseudallescheria apiosperma sp. nov.
Medical mycology, 2010Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:Scedosporium apiospermum has traditionally been thought of as the anamorph of Pseudallescheria boydii (Microascaceae, Ascomycota), but recent molecular studies has demonstrated that they are different species. Since a teleomorph was not observed among isolates recently identified as S. apiospermum, we investigated whether this species could be heterothallic. In this study, 15 isolates of S. apiospermum were paired in all possible combinations, including self-pairings. Several combinations produced fertile ascomata typical of the genus Pseudallescheria, while all isolates were self-sterile. The isolates were grouped into two different mating types. Crosses among F1 progeny ascospores demonstrated a bi-allelic heterothallic mating system. The new species Pseudallescheria apiosperma, teleomorph of S. apiospermum, is proposed and described.
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molecular and phenotypic data supporting distinct species statuses for scedosporium apiospermum and Pseudallescheria boydii and the proposed new species scedosporium dehoogii
Journal of Clinical Microbiology, 2008Co-Authors: Felix Gilgado, Josep Cano, Deanna A. Sutton, Josepa Gené, Josep GuarroAbstract:Based on the morphological, physiologic, and molecular (β-tubulin gene) study of 141 isolates of the Pseudallescheria boydii species complex (including several synonyms) and relatives, the new species Scedosporium dehoogii is proposed. Scedosporium apiospermum and P. boydii are considered two different species and the new name Scedosporium boydii is proposed for the anamorph of the latter species. A summary of the key morphological and physiological features for distinguishing the species of Pseudallescheria/Scedosporium is provided.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. β-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84T, CBS 311.72T and CBS 129.78T.
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Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Felix Gilgado, Josep Cano, Josepa Gené, Josep GuarroAbstract:During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. beta-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84(T), CBS 311.72(T) and CBS 129.78(T).
