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Klaus-diete Schlüte - One of the best experts on this subject based on the ideXlab platform.
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes - Fig 1
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:De- and redifferentiation of ARVC in culture with 20% FCS (A) Freshly isolated ARVC with their typical rod shape form (Day 0) completely rounded down by degrading sarcomeres starting in the periphery (Day 1) in the first days of culture. They lost all their contractile elements (Day 2) followed by formation of Pseudopodia-like structures (spreading; Day 3–5) and subsequent reformation of their contractile elements indicating de novo sarcomerogenesis (Day 6). At day six in culture, cross striation was clearly detectable again. (B) Increase in cardiomyocytes with Pseudopodia-like structures normalized to all counted cardiomyocytes (spreading in %) during cultivation time (n = 33 cell preparations). Data are presented as means ± SEM
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:BackgroundRecent findings suggest that adult terminally differentiated cardiomyocytes adapt to stress by cellular de- and redifferentiation. In the present study we tested the hypothesis that swiprosin-1 is a key player in this process. Furthermore, the relationship between swiprosin-1 and β-adrenoceptor coupling was analyzed.MethodsIn order to study the function of swiprosin-1 in adult rat ventricular cardiomyocytes (ARVC) they were isolated and cultured in a medium containing 20% fetal calf serum (FCS). Changes in cell morphology of ARVC during cultivation were quantified by light and confocal laser scan microscopy. Small interfering RNA (siRNA) was used to reduce the expression of swiprosin-1. The impact of calcium on swiprosin-1 dependent processes was investigated with Bapta-AM. Immunoblot techniques and qRT-PCR were performed to measure mRNA and protein expression.ResultsIn culture, ARVC first lost their contractile elements, which was followed by a formation of Pseudopodia-like structures (spreading). Swiprosin-1 was detected in ARVC at all time points. However, swiprosin-1 expression was increased when ARVC started to spread. Reduction of swiprosin-1 expression with siRNA delayed ARVC spreading. Similarly, Bapta-AM attenuated swiprosin-1 expression and spreading of ARVC. Furthermore, swiprosin-1 expression correlated with the expression of G protein-coupled receptor kinase 2 (GRK2). Moreover, silencing of swiprosin-1 was associated with a down regulation of GRK2 and caused a sensitization of β-adrenergic receptors.ConclusionSwiprosin-1 is required for ARVC to adapt to culture conditions. Additionally, it seems to be involved in the desensitization of β-adrenergic receptors. Assuming that ARVC adapt to cardiac stress in a similar way, swiprosin-1 may play a key role in cardiac remodeling.
Franziska Nippe - One of the best experts on this subject based on the ideXlab platform.
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes - Fig 1
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:De- and redifferentiation of ARVC in culture with 20% FCS (A) Freshly isolated ARVC with their typical rod shape form (Day 0) completely rounded down by degrading sarcomeres starting in the periphery (Day 1) in the first days of culture. They lost all their contractile elements (Day 2) followed by formation of Pseudopodia-like structures (spreading; Day 3–5) and subsequent reformation of their contractile elements indicating de novo sarcomerogenesis (Day 6). At day six in culture, cross striation was clearly detectable again. (B) Increase in cardiomyocytes with Pseudopodia-like structures normalized to all counted cardiomyocytes (spreading in %) during cultivation time (n = 33 cell preparations). Data are presented as means ± SEM
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:BackgroundRecent findings suggest that adult terminally differentiated cardiomyocytes adapt to stress by cellular de- and redifferentiation. In the present study we tested the hypothesis that swiprosin-1 is a key player in this process. Furthermore, the relationship between swiprosin-1 and β-adrenoceptor coupling was analyzed.MethodsIn order to study the function of swiprosin-1 in adult rat ventricular cardiomyocytes (ARVC) they were isolated and cultured in a medium containing 20% fetal calf serum (FCS). Changes in cell morphology of ARVC during cultivation were quantified by light and confocal laser scan microscopy. Small interfering RNA (siRNA) was used to reduce the expression of swiprosin-1. The impact of calcium on swiprosin-1 dependent processes was investigated with Bapta-AM. Immunoblot techniques and qRT-PCR were performed to measure mRNA and protein expression.ResultsIn culture, ARVC first lost their contractile elements, which was followed by a formation of Pseudopodia-like structures (spreading). Swiprosin-1 was detected in ARVC at all time points. However, swiprosin-1 expression was increased when ARVC started to spread. Reduction of swiprosin-1 expression with siRNA delayed ARVC spreading. Similarly, Bapta-AM attenuated swiprosin-1 expression and spreading of ARVC. Furthermore, swiprosin-1 expression correlated with the expression of G protein-coupled receptor kinase 2 (GRK2). Moreover, silencing of swiprosin-1 was associated with a down regulation of GRK2 and caused a sensitization of β-adrenergic receptors.ConclusionSwiprosin-1 is required for ARVC to adapt to culture conditions. Additionally, it seems to be involved in the desensitization of β-adrenergic receptors. Assuming that ARVC adapt to cardiac stress in a similar way, swiprosin-1 may play a key role in cardiac remodeling.
Schlüte Klaus-diete - One of the best experts on this subject based on the ideXlab platform.
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes
Public Library of Science, 2016Co-Authors: Nippe Franziska, Schreckenberg Rolf, Hess Antonia, Webe Marti, Schlüte Klaus-dieteAbstract:Recent findings suggest that adult terminally differentiated cardiomyocytes adapt to stress by cellular de- and redifferentiation. In the present study we tested the hypothesis that swiprosin-1 is a key player in this process. Furthermore, the relationship between swiprosin-1 and β-adrenoceptor coupling was analyzed.In order to study the function of swiprosin-1 in adult rat ventricular cardiomyocytes (ARVC) they were isolated and cultured in a medium containing 20% fetal calf serum (FCS). Changes in cell morphology of ARVC during cultivation were quantified by light and confocal laser scan microscopy. Small interfering RNA (siRNA) was used to reduce the expression of swiprosin-1. The impact of calcium on swiprosin-1 dependent processes was investigated with Bapta-AM. Immunoblot techniques and qRT-PCR were performed to measure mRNA and protein expression.In culture, ARVC first lost their contractile elements, which was followed by a formation of Pseudopodia-like structures (spreading). Swiprosin-1 was detected in ARVC at all time points. However, swiprosin-1 expression was increased when ARVC started to spread. Reduction of swiprosin-1 expression with siRNA delayed ARVC spreading. Similarly, Bapta-AM attenuated swiprosin-1 expression and spreading of ARVC. Furthermore, swiprosin-1 expression correlated with the expression of G protein-coupled receptor kinase 2 (GRK2). Moreover, silencing of swiprosin-1 was associated with a down regulation of GRK2 and caused a sensitization of β-adrenergic receptors.Swiprosin-1 is required for ARVC to adapt to culture conditions. Additionally, it seems to be involved in the desensitization of β-adrenergic receptors. Assuming that ARVC adapt to cardiac stress in a similar way, swiprosin-1 may play a key role in cardiac remodeling
Andrew J. Gooday - One of the best experts on this subject based on the ideXlab platform.
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High diversity of deep-sea Gromia from the Arabian Sea revealed by small subunit rDNA sequence analysis
Marine Biology, 2006Co-Authors: Ana Aranda Da Silva, Jan Pawlowski, Andrew J. GoodayAbstract:Gromia is a large marine protist with filose Pseudopodia and ovoid test, common in coastal intertidal and sublittoral waters. Although deep-water Gromia -like morphospecies were discovered in the 1990s, their relations to the shallow water species have never been established. Moreover, very little is known about the diversity within Gromia , reflecting the fact that these morphologically relatively simple protists have few characters useful for species identification. Consequently, we have analysed the SSU rDNA and ITS rDNA genes to examine gromiid diversity in two different areas located on the Oman and Pakistan margins of the Arabian Sea (water depths 1,000–2,000 m). In total, 27 deep-sea gromiid sequences of the SSU rDNA gene and six sequences of the ITS rDNA region were obtained. Our data confirm that Gromia -like protists from the bathyal deep sea are related to the shallow-water gromiids. Within Arabian Sea Gromia , we identified seven distinctive lineages, five of which form a monophyletic group branching as a sister group to shallow-water species. Six lineages of Arabian Sea Gromia can be defined morphologically, while one lineage includes specimens that look identical to specimens from two other lineages. This indicates that each Gromia lineage represents probably a separate species and suggests that deep-sea gromiid diversity is higher than indicated by their simple morphology.
Antonia Hess - One of the best experts on this subject based on the ideXlab platform.
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes - Fig 1
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:De- and redifferentiation of ARVC in culture with 20% FCS (A) Freshly isolated ARVC with their typical rod shape form (Day 0) completely rounded down by degrading sarcomeres starting in the periphery (Day 1) in the first days of culture. They lost all their contractile elements (Day 2) followed by formation of Pseudopodia-like structures (spreading; Day 3–5) and subsequent reformation of their contractile elements indicating de novo sarcomerogenesis (Day 6). At day six in culture, cross striation was clearly detectable again. (B) Increase in cardiomyocytes with Pseudopodia-like structures normalized to all counted cardiomyocytes (spreading in %) during cultivation time (n = 33 cell preparations). Data are presented as means ± SEM
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The Effects of Swiprosin-1 on the Formation of Pseudopodia-Like Structures and β-Adrenoceptor Coupling in Cultured Adult Rat Ventricular Cardiomyocytes
2016Co-Authors: Franziska Nippe, Rolf Schreckenberg, Antonia Hess, Marti Webe, Klaus-diete SchlüteAbstract:BackgroundRecent findings suggest that adult terminally differentiated cardiomyocytes adapt to stress by cellular de- and redifferentiation. In the present study we tested the hypothesis that swiprosin-1 is a key player in this process. Furthermore, the relationship between swiprosin-1 and β-adrenoceptor coupling was analyzed.MethodsIn order to study the function of swiprosin-1 in adult rat ventricular cardiomyocytes (ARVC) they were isolated and cultured in a medium containing 20% fetal calf serum (FCS). Changes in cell morphology of ARVC during cultivation were quantified by light and confocal laser scan microscopy. Small interfering RNA (siRNA) was used to reduce the expression of swiprosin-1. The impact of calcium on swiprosin-1 dependent processes was investigated with Bapta-AM. Immunoblot techniques and qRT-PCR were performed to measure mRNA and protein expression.ResultsIn culture, ARVC first lost their contractile elements, which was followed by a formation of Pseudopodia-like structures (spreading). Swiprosin-1 was detected in ARVC at all time points. However, swiprosin-1 expression was increased when ARVC started to spread. Reduction of swiprosin-1 expression with siRNA delayed ARVC spreading. Similarly, Bapta-AM attenuated swiprosin-1 expression and spreading of ARVC. Furthermore, swiprosin-1 expression correlated with the expression of G protein-coupled receptor kinase 2 (GRK2). Moreover, silencing of swiprosin-1 was associated with a down regulation of GRK2 and caused a sensitization of β-adrenergic receptors.ConclusionSwiprosin-1 is required for ARVC to adapt to culture conditions. Additionally, it seems to be involved in the desensitization of β-adrenergic receptors. Assuming that ARVC adapt to cardiac stress in a similar way, swiprosin-1 may play a key role in cardiac remodeling.
