The Experts below are selected from a list of 13836 Experts worldwide ranked by ideXlab platform
Laurent J. Feldman - One of the best experts on this subject based on the ideXlab platform.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:1 - ArticleIn the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA.
Journal of biomedical materials research. Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc.
Aurélie San Juan - One of the best experts on this subject based on the ideXlab platform.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:1 - ArticleIn the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA.
Journal of biomedical materials research. Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc.
Grégory Ducrocq - One of the best experts on this subject based on the ideXlab platform.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:1 - ArticleIn the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA.
Journal of biomedical materials research. Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc.
Hanna Hlawaty - One of the best experts on this subject based on the ideXlab platform.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:1 - ArticleIn the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA.
Journal of biomedical materials research. Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc.
Isabelle Bataille - One of the best experts on this subject based on the ideXlab platform.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:1 - ArticleIn the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA.
Journal of biomedical materials research. Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
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Tubular cationized Pullulan hydrogels as local reservoirs for plasmid DNA
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Aurélie San Juan, Grégory Ducrocq, Hanna Hlawaty, Isabelle Bataille, Erwann Guénin, Didier Letourneur, Laurent J. FeldmanAbstract:In the present study, we measured the ability of various cationized Pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized Pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethyl-amine (DEAE) on the Pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized Pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized Pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized Pullulan hydrogels. Native Pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized Pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized Pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized Pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies. (c) 2007 Wiley Periodicals, Inc.
