The Experts below are selected from a list of 3012 Experts worldwide ranked by ideXlab platform
Liu Guiqing - One of the best experts on this subject based on the ideXlab platform.
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expression and significance of transforming growth factor β_1 and c myc gene in the Pulmonary Blood Vessel of patients with congenital heart disease associated with Pulmonary hypertension
Shandong Medical Journal, 2007Co-Authors: Liu GuiqingAbstract:[Objective] To study the expression of transforming growth factor β1(TGF-β1) and C-MYC gene in the Pulmonary Blood Vessel of patients with congenital heart disease(CHD) associated with Pulmonary hypertension(PH),and their relationship with pathological behavior of grading and staging of PH.To explore their roles in the genesis of CHD complicated with PH.[Methods] 112 patients with CHD were divided into three groups: no-PH group(33patients),with preoperative mean Pulmonary artery pressure(MPAP) 20 mmHg;PH group(55patients),with preoperative MPAP20 mmHg,and cyanosis group(24patients),with preoperative MPAP70 mmHg.The lung tissue samples were examined from 112 patients by surgical biopsy of lung.Lung tissue was examined for expression of TGF-β1 and C-MYC by immunohistochemical technique.Pathological grading of Pulmonary vascular changes was standardized by Heath-Edwards.[Results] TGF-β1 was found mainly in the cytoplasm of endotheliocyte,smooth muscle cells and fibroblasts of Pulmonary artery.C-MYC gene was found mainly in the karyon of endotheliocytes,smooth muscle cells and fibroblasts of Pulmonary artery.The intensity(A) of TGF-β1 and C-MYC expression in the lung tissues of PH groups was increased gradually with the grading increasing.The intensity expression(A)of TGF-β1 and C-MYC in the lung tissues in all pathological degree groups showed statistic differences.The expression of TGF-β1 and C-MYC had significant correlation with pathological grade.[Conclusion] TGF-β1 and C-MYC gene have an important biological behavior in the genesis of PH of CHD.TGF-β1 induces C-MYC gene positively.They improve the proliferation of smooth muscle cell and fibroblasts.
Raul San Jose Estepar - One of the best experts on this subject based on the ideXlab platform.
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Pulmonary artery enlargement is associated with right ventricular dysfunction and loss of Blood volume in small Pulmonary Vessels in chronic obstructive Pulmonary disease
Circulation-cardiovascular Imaging, 2015Co-Authors: Michael J Wells, Anand S Iyer, Farbod N Rahaghi, Surya P Bhatt, Himanshu Gupta, Thomas S Denney, Steven G Lloyd, Louis J Dellitalia, Hrudaya Nath, Raul San Jose EsteparAbstract:Background— Chronic obstructive Pulmonary disease causes significant morbidity and concomitant Pulmonary vascular disease and cardiac dysfunction are associated with poor prognosis. Computed tomography–detected relative Pulmonary artery (PA) enlargement defined as a PA to ascending aorta diameter ratio >1 (PA:A>1) is a marker for Pulmonary hypertension and predicts chronic obstructive Pulmonary disease exacerbations. However, little is known about the relationship between the PA:A ratio, Pulmonary Blood volume, and cardiac function. Methods and Results— A single-center prospective cohort study of patients with chronic obstructive Pulmonary disease was conducted. Clinical characteristics and computed tomography metrics, including the PA:A and Pulmonary Blood Vessel volume, were measured. Ventricular functions, volumes, and dimensions were measured by cine cardiac MRI with 3-dimensional analysis. Linear regression examined the relationships between clinical characteristics, computed tomography and cardiac MRI metrics, and 6-minute walk distance. Twenty-four patients were evaluated and those with PA:A>1 had higher right ventricular (RV) end-diastolic and end-systolic volume indices accompanied by lower RV ejection fraction (52±7% versus 60±9%; P =0.04). The PA:A correlated inversely with total intraparenchymal Pulmonary Blood Vessel volume and the volume of distal Vessels with a cross-sectional area of 1, and hyperinflation correlated with reduced RV ejection fraction. Both PA diameter and reduced RV ejection fraction were independently associated with reduced 6-minute walk distance. Conclusions— The loss of Blood volume in distal Pulmonary Vessels is associated with PA enlargement on computed tomography. Cardiac MRI detects early RV dysfunction and remodeling in nonsevere chronic obstructive Pulmonary disease patients with a PA:A>1. Both RV dysfunction and PA enlargement are independently associated with reduced walk distance. Clinical Trial Registration— URL: . Unique identifier: [NCT00608764][1]. [1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT00608764&atom=%2Fcirccvim%2F8%2F4%2Fe002546.atom
Tetsuya Kodama - One of the best experts on this subject based on the ideXlab platform.
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analysis of tumor vascularization in a mouse model of metastatic lung cancer
Scientific Reports, 2019Co-Authors: Ariunbuyan Sukhbaatar, Maya Sakamoto, Shiro Mori, Tetsuya KodamaAbstract:Therapies targeting tumor vasculature would improve the treatment of lung metastasis, although the early changes in vascular structure are incompletely understood. Here, we show that obstructive metastatic foci in lung arterioles decrease the Pulmonary vascular network. To generate a mouse model of lung metastasis activation, luciferase-expressing tumor cells were inoculated into the subiliac lymph node (SiLN) of an MXH10/Mo-lpr/lpr mouse, and metastatic tumor cells in the lungs were activated by SiLN resection. Activation of metastases was monitored by in vivo bioluminescence imaging. Pulmonary Blood Vessel characteristics were analyzed using ex vivo micro-computed tomography. The enhanced permeability and retention (EPR) effect in neovasculature after tumor cell activation was evaluated from the accumulation of intravenously injected indocyanine green (ICG) liposomes. Metastatic foci in lung arterioles were investigated histologically. Micro-computed tomography revealed decreases in Pulmonary Blood Vessel length, volume and number of branching nodes during the early stage of metastasis caused by metastatic foci. ICG liposome accumulation by the EPR effect was not detected. Histology identified metastatic foci in lung arterioles. The lack of an EPR effect after the formation of metastatic foci in lung arterioles makes conventional systemic chemotherapy ineffective for lung metastasis. Thus, alternative therapeutic methods of drug delivery are needed.
Michael J Wells - One of the best experts on this subject based on the ideXlab platform.
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Pulmonary artery enlargement is associated with right ventricular dysfunction and loss of Blood volume in small Pulmonary Vessels in chronic obstructive Pulmonary disease
Circulation-cardiovascular Imaging, 2015Co-Authors: Michael J Wells, Anand S Iyer, Farbod N Rahaghi, Surya P Bhatt, Himanshu Gupta, Thomas S Denney, Steven G Lloyd, Louis J Dellitalia, Hrudaya Nath, Raul San Jose EsteparAbstract:Background— Chronic obstructive Pulmonary disease causes significant morbidity and concomitant Pulmonary vascular disease and cardiac dysfunction are associated with poor prognosis. Computed tomography–detected relative Pulmonary artery (PA) enlargement defined as a PA to ascending aorta diameter ratio >1 (PA:A>1) is a marker for Pulmonary hypertension and predicts chronic obstructive Pulmonary disease exacerbations. However, little is known about the relationship between the PA:A ratio, Pulmonary Blood volume, and cardiac function. Methods and Results— A single-center prospective cohort study of patients with chronic obstructive Pulmonary disease was conducted. Clinical characteristics and computed tomography metrics, including the PA:A and Pulmonary Blood Vessel volume, were measured. Ventricular functions, volumes, and dimensions were measured by cine cardiac MRI with 3-dimensional analysis. Linear regression examined the relationships between clinical characteristics, computed tomography and cardiac MRI metrics, and 6-minute walk distance. Twenty-four patients were evaluated and those with PA:A>1 had higher right ventricular (RV) end-diastolic and end-systolic volume indices accompanied by lower RV ejection fraction (52±7% versus 60±9%; P =0.04). The PA:A correlated inversely with total intraparenchymal Pulmonary Blood Vessel volume and the volume of distal Vessels with a cross-sectional area of 1, and hyperinflation correlated with reduced RV ejection fraction. Both PA diameter and reduced RV ejection fraction were independently associated with reduced 6-minute walk distance. Conclusions— The loss of Blood volume in distal Pulmonary Vessels is associated with PA enlargement on computed tomography. Cardiac MRI detects early RV dysfunction and remodeling in nonsevere chronic obstructive Pulmonary disease patients with a PA:A>1. Both RV dysfunction and PA enlargement are independently associated with reduced walk distance. Clinical Trial Registration— URL: . Unique identifier: [NCT00608764][1]. [1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT00608764&atom=%2Fcirccvim%2F8%2F4%2Fe002546.atom
Ariunbuyan Sukhbaatar - One of the best experts on this subject based on the ideXlab platform.
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analysis of tumor vascularization in a mouse model of metastatic lung cancer
Scientific Reports, 2019Co-Authors: Ariunbuyan Sukhbaatar, Maya Sakamoto, Shiro Mori, Tetsuya KodamaAbstract:Therapies targeting tumor vasculature would improve the treatment of lung metastasis, although the early changes in vascular structure are incompletely understood. Here, we show that obstructive metastatic foci in lung arterioles decrease the Pulmonary vascular network. To generate a mouse model of lung metastasis activation, luciferase-expressing tumor cells were inoculated into the subiliac lymph node (SiLN) of an MXH10/Mo-lpr/lpr mouse, and metastatic tumor cells in the lungs were activated by SiLN resection. Activation of metastases was monitored by in vivo bioluminescence imaging. Pulmonary Blood Vessel characteristics were analyzed using ex vivo micro-computed tomography. The enhanced permeability and retention (EPR) effect in neovasculature after tumor cell activation was evaluated from the accumulation of intravenously injected indocyanine green (ICG) liposomes. Metastatic foci in lung arterioles were investigated histologically. Micro-computed tomography revealed decreases in Pulmonary Blood Vessel length, volume and number of branching nodes during the early stage of metastasis caused by metastatic foci. ICG liposome accumulation by the EPR effect was not detected. Histology identified metastatic foci in lung arterioles. The lack of an EPR effect after the formation of metastatic foci in lung arterioles makes conventional systemic chemotherapy ineffective for lung metastasis. Thus, alternative therapeutic methods of drug delivery are needed.
