The Experts below are selected from a list of 201 Experts worldwide ranked by ideXlab platform
Heli Sirén - One of the best experts on this subject based on the ideXlab platform.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
Sari Metsämuuronen - One of the best experts on this subject based on the ideXlab platform.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
Katja Lyytikäinen - One of the best experts on this subject based on the ideXlab platform.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
Kaj Backfolk - One of the best experts on this subject based on the ideXlab platform.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
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Determination of xylo-oligosaccharides in enzymatically hydrolysed Pulp by liquid chromatography and capillary electrophoresis
Cellulose, 2013Co-Authors: Sari Metsämuuronen, Katja Lyytikäinen, Kaj Backfolk, Heli SirénAbstract:Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft Pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft Pulp when the bleached Material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the Pulp Material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft Pulp.
Meiji Okamoto - One of the best experts on this subject based on the ideXlab platform.
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the use of fungal inoculants in the ensiling of potato Pulp effect of temperature and duration of storage on silage fermentation characteristics
Asian-australasian Journal of Animal Sciences, 2006Co-Authors: A Okine, Y Aibibula, Masaaki Hanada, Meiji OkamotoAbstract:A 3x3 factorial design experiment was conducted to investigate the effect of temperature and duration of storage on the fermentation quality of potato Pulp ensiled with two fungal inoculants under laboratory conditions. The inoculants, Rhizopus oryzae (R) and Amylomyces rouxii (A) were each added to potato Pulp Material to contain at least 1×10 6 CFU/g fresh matter, and silages without additives served as controls. The silages were stored under three temperature regimes; 4, 12 and 25°C. Three silos per treatment from every temperature regime were opened on days 7, 24 and 40 days after ensiling to investigate treatment effects on fermentation quality, starch and sugar concentrations. Increase in temperature and duration of storage had a positive significant effect (p 0.05) on the fermentation quality of the silages. Sugar concentration in the silages decreased with increase in temperature (p<0.01) but increased (p<0.05) with progression of duration of storage. The fungal inoculants had no effect on starch degradation in PPS. The results suggest that storage temperature and duration of storage are more important in determining the rate of fermentation than addition of the fungal inoculants in PPS.
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The Use of Fungal Inoculants in the Ensiling of Potato Pulp: Effect of Temperature and Duration of Storage on Silage Fermentation Characteristics
Asian-Australasian Journal of Animal Sciences, 2006Co-Authors: A Okine, Y Aibibula, Masaaki Hanada, Meiji OkamotoAbstract:A 3x3 factorial design experiment was conducted to investigate the effect of temperature and duration of storage on the fermentation quality of potato Pulp ensiled with two fungal inoculants under laboratory conditions. The inoculants, Rhizopus oryzae (R) and Amylomyces rouxii (A) were each added to potato Pulp Material to contain at least 1×10 6 CFU/g fresh matter, and silages without additives served as controls. The silages were stored under three temperature regimes; 4, 12 and 25°C. Three silos per treatment from every temperature regime were opened on days 7, 24 and 40 days after ensiling to investigate treatment effects on fermentation quality, starch and sugar concentrations. Increase in temperature and duration of storage had a positive significant effect (p 0.05) on the fermentation quality of the silages. Sugar concentration in the silages decreased with increase in temperature (p
