The Experts below are selected from a list of 49968 Experts worldwide ranked by ideXlab platform
Timothy J Barrett - One of the best experts on this subject based on the ideXlab platform.
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rapid pulsed field Gel Electrophoresis protocol for subtyping of campylobacter jejuni
Journal of Clinical Microbiology, 2001Co-Authors: Efrain M Ribot, Collette Fitzgerald, Kristy Kubota, Bala Swaminathan, Timothy J BarrettAbstract:We developed a rapid Pulsed-Field Gel Electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.
Jim Manos - One of the best experts on this subject based on the ideXlab platform.
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Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa
Methods in molecular biology (Clifton N.J.), 2015Co-Authors: Jim ManosAbstract:Pulsed-Field Gel Electrophoresis (PFGE) is a technique used for the separation of high molecular weight restriction fragments from digested bacterial genomic DNA on a Gel matrix by applying an electric field that periodically changes direction. PFGE has been shown to be the most discriminatory and reproducible bacterial strain typing technique available. It is considered the 'gold standard' in epidemiological studies of pathogenic and nonpathogenic microorganisms. This chapter provides a detailed method for using PFGE in the genotyping of the opportunistic Gram-negative bacterial pathogen Pseudomonas aeruginosa.
D. Hocquet - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Pulsed-Field Gel Electrophoresis and whole-genome-sequencing-based typing confirms the accuracy of Pulsed-Field Gel Electrophoresis for the investigation of local Pseudomonas aeruginosa outbreaks
Journal of Hospital Infection, 2020Co-Authors: D. Martak, A. Meunier, M. Sauget, P. Cholley, M. Thouverez, X. Bertrand, B. Valot, D. HocquetAbstract:To determine whether Pulsed-Field Gel Electrophoresis (PFGE) accurately recognizes isolates belonging to clusters defined by techniques based on whole-genome sequencing (WGS) using Pseudomonas aeruginosa as a model.
E. John Threlfall - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis by plasmid analysis and Pulsed-Field Gel Electrophoresis
International journal of antimicrobial agents, 2007Co-Authors: Zerrin Aktas, Martin Day, Cigdem Bal Kayacan, Sukufe Diren, E. John ThrelfallAbstract:Abstract Forty-one paediatric isolates of Salmonella spp. from Cerrahpasa Faculty of Medicine, Istanbul, Turkey, between 2001 and 2004 were examined for susceptibility to various antibiotics and presence of antibiotic resistance genes. Pulsed-Field Gel Electrophoresis and plasmid profiling were used to determine possible genetic relationships among Salmonella enterica subsp. enterica clinical isolates. Plasmids from resistant strains were not transferred by conjugation to recipient Escherichia coli cells. Pulsed-Field Gel Electrophoresis and restriction enzyme digestion analysis of DNA revealed that multidrug-resistant isolates belonged to the same clonal group, characterized by ACSSuT resistotype. Isolates of R-type ACSSuT were positive for the intI gene and possessed a single plasmid of 60MDa.
Efrain M Ribot - One of the best experts on this subject based on the ideXlab platform.
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rapid pulsed field Gel Electrophoresis protocol for subtyping of campylobacter jejuni
Journal of Clinical Microbiology, 2001Co-Authors: Efrain M Ribot, Collette Fitzgerald, Kristy Kubota, Bala Swaminathan, Timothy J BarrettAbstract:We developed a rapid Pulsed-Field Gel Electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.
