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Carla N. Alvarez - One of the best experts on this subject based on the ideXlab platform.
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Redescription of the female, male, larva and Pupa of Sabethes (Sabethoides) glaucodaemon (Dyar & Shannon) (Diptera: Culicidae) and description of the female genitalia
Zootaxa, 2020Co-Authors: Marina Stein, Maycon Sebastião Alberto Santos Neves, Debora N. Bangher, Carla N. AlvarezAbstract:Sabethes ( Sabethoides ) glaucodaemon was described for the first time by Dyar & Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and Pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and Pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the Pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa . ( Sbo .) tridentatus are discussed.
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redescription of the female male larva and Pupa of sabethes sabethoides glaucodaemon dyar shannon diptera culicidae and description of the female genitalia
Zootaxa, 2020Co-Authors: Marina Stein, Maycon Sebastião Alberto Santos Neves, Debora N. Bangher, Carla N. AlvarezAbstract:Sabethes ( Sabethoides ) glaucodaemon was described for the first time by Dyar & Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and Pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and Pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the Pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa . ( Sbo .) tridentatus are discussed.
Luis A. Quesada-allué - One of the best experts on this subject based on the ideXlab platform.
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White Pupa: a Ceratitis capitata mutant lacking catecholamines for tanning the Puparium
Insect Biochemistry and Molecular Biology, 1995Co-Authors: Pablo Wappner, Karl J. Kramer, Theodore L. Hopkins, Matthew E. Merritt, Jacob Schaefer, Luis A. Quesada-alluéAbstract:Abstract The white Pupa mutant of the Mediterranean fruit fly, Ceratitis capitata , fails to tan the Puparium, but develops normal larval and adult cuticular structures. We found that the Puparium of this mutant underwent minor stiffening at the beginning of Pupariation, but subsequently did not increase further in stiffness. By the end of poparium formation, it was fivefold less resistant to compression than the wild type strain, Scanning electron microscopy of cross-sections of Puparial exuviae revealed a dense sclerotized cuticle in the wild type, whereas the white Pupa cuticle was quite distinct, with the inner two-thirds consisting of unsclerotized lamellae and the outer third being a dense, nonlaminar, amorphous layer. Puparial catecholamine levels were also very low in the white Pupa when compared with the wild type strain, in which N -β-alanyldopamine (NBAD) predominated. However, in mutant hemolymph, NBAD, N -acetyldopamine (NADA), and dopamine were about 10 times more concentrated than in the normal phenotype. By injecting 1- 14 C-β-alanine as a tracer, we confirmed that N -β-alanyldopamine incorporation into the Puparium was much lower in the white Pupa than in the wild type strain. However, insoluble cuticle phenoloxidase activity was similar in the two strains. Tanning occurred in vitro when white Pupa Puparial cuticle, free of epidermis, was incubated with either NBAD or NADA, and melanization occurred when the cuticle was incubated with dopamine, demonstrating that tanning enzymes, but not substrates, were present in white Pupa Puparial cuticle. Solid state 3 C nuclear magnetic resonance spectroscopy revealed that more chitin as well as less protein, catechols and β-alanine were present in the white Pupa cuticle relative to the wild type. We conclude that the white Pupa mutant is defective in the mechanism that provides hemolymph catecholamines to the Puparial cuticle; this defect prevents normal sclerotization and pigmentation.
Marina Stein - One of the best experts on this subject based on the ideXlab platform.
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Redescription of the female, male, larva and Pupa of Sabethes (Sabethoides) glaucodaemon (Dyar & Shannon) (Diptera: Culicidae) and description of the female genitalia
Zootaxa, 2020Co-Authors: Marina Stein, Maycon Sebastião Alberto Santos Neves, Debora N. Bangher, Carla N. AlvarezAbstract:Sabethes ( Sabethoides ) glaucodaemon was described for the first time by Dyar & Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and Pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and Pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the Pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa . ( Sbo .) tridentatus are discussed.
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redescription of the female male larva and Pupa of sabethes sabethoides glaucodaemon dyar shannon diptera culicidae and description of the female genitalia
Zootaxa, 2020Co-Authors: Marina Stein, Maycon Sebastião Alberto Santos Neves, Debora N. Bangher, Carla N. AlvarezAbstract:Sabethes ( Sabethoides ) glaucodaemon was described for the first time by Dyar & Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and Pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and Pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the Pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa . ( Sbo .) tridentatus are discussed.
Pablo Wappner - One of the best experts on this subject based on the ideXlab platform.
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White Pupa: a Ceratitis capitata mutant lacking catecholamines for tanning the Puparium
Insect Biochemistry and Molecular Biology, 1995Co-Authors: Pablo Wappner, Karl J. Kramer, Theodore L. Hopkins, Matthew E. Merritt, Jacob Schaefer, Luis A. Quesada-alluéAbstract:Abstract The white Pupa mutant of the Mediterranean fruit fly, Ceratitis capitata , fails to tan the Puparium, but develops normal larval and adult cuticular structures. We found that the Puparium of this mutant underwent minor stiffening at the beginning of Pupariation, but subsequently did not increase further in stiffness. By the end of poparium formation, it was fivefold less resistant to compression than the wild type strain, Scanning electron microscopy of cross-sections of Puparial exuviae revealed a dense sclerotized cuticle in the wild type, whereas the white Pupa cuticle was quite distinct, with the inner two-thirds consisting of unsclerotized lamellae and the outer third being a dense, nonlaminar, amorphous layer. Puparial catecholamine levels were also very low in the white Pupa when compared with the wild type strain, in which N -β-alanyldopamine (NBAD) predominated. However, in mutant hemolymph, NBAD, N -acetyldopamine (NADA), and dopamine were about 10 times more concentrated than in the normal phenotype. By injecting 1- 14 C-β-alanine as a tracer, we confirmed that N -β-alanyldopamine incorporation into the Puparium was much lower in the white Pupa than in the wild type strain. However, insoluble cuticle phenoloxidase activity was similar in the two strains. Tanning occurred in vitro when white Pupa Puparial cuticle, free of epidermis, was incubated with either NBAD or NADA, and melanization occurred when the cuticle was incubated with dopamine, demonstrating that tanning enzymes, but not substrates, were present in white Pupa Puparial cuticle. Solid state 3 C nuclear magnetic resonance spectroscopy revealed that more chitin as well as less protein, catechols and β-alanine were present in the white Pupa cuticle relative to the wild type. We conclude that the white Pupa mutant is defective in the mechanism that provides hemolymph catecholamines to the Puparial cuticle; this defect prevents normal sclerotization and pigmentation.
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White Pupa: a Ceratitb3 capitata Mutant Lacking Catecholamines for Tanning the
1995Co-Authors: Pablo Wappner, Karl J. Kramer, Theodore L. Hopkins, Matthew E. MerrittAbstract:The white Pupa mutant of the Mediterranean fruit fly, Ceratitis capitata, fails to tan the Puparium, but develops normal larval and adult cuticular structures. We found that the Puparium of this mutant underwent minor stiffening at the beginning of Pupariation, but subsequently did not increase further in stiffness. By the end of Puparium formation, it was fivefoid less resistant to compression than the wild type strain. Scanning electron microscopy of cross-sections of Puparial exuviae revealed a dense sclerotized cuticle in the wild type, whereas the white Pupa cuticle was quite distinct, with the inner two-thirds consisting of unsclerotized lamellae and the outer third being a dense, nonlaminar, amorphous layer. Puparial catecholamine levels were also very low in the white Pupa when compared with the wild type strain, in which N-~-alanyldopamine (NBAD) predominated. However, in mutant hemolymph, NBAD, N-acetyldopamine (NADA), and dopamine were about 10 times more concentrated than in the normal phenotype. By injecting 1-14C-~-alanine as a tracer, we confirmed that N-~-alanyldopamine incorporation into the Puparium was much lower in the white Pupa than in the wild type strain. However, insoluble cuticle phenoloxidase activity was similar in the two strains. Tanning occurred in vitro when white Pupa Puparial cuticle, free of epidermis, was incubated with either NBAD or NADA, and melanization occurred when the cuticle was incubated with dopamine, demonstrating that tanning enzymes, but not substrates, were present in white Pupa Puparial cuticle. Solid state '3C nuclear magnetic resonance spectroscopy revealed that more chitin as well as less protein, catechois and ~-alanine were present in the white Pupa cuticle relative to the wild type. We conclude that the white Pupa mutant is defective in the mechanism that provides hemolymph catecholamines to the Puparial cuticle; this defect prevents normal sclerotization and pigmentation.
Jiang Yan-zhong - One of the best experts on this subject based on the ideXlab platform.
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Supercritical CO_2 Extraction of Silkworm Pupa Oil
Technology & Development of Chemical Industry, 2013Co-Authors: Jiang Yan-zhongAbstract:The extraction of silkworm Pupa oil by supercritical fluid CO 2 was studied.Based on the single factor experiment by orthogonal experiment,the effects of the parameters such as temperature,pressure and time on the yield of silkworm Pupa oil were studied with 8kg/h of CO 2 flow.The results showed that the extraction temperature was the more important factor that affected the effect of extraction,following the extraction time and pressure.The optimal conditions of super critical fluid CO 2 were: extracting temperature 50℃,pressure 25MPa,time 2.5h.Under these conditions the yield of silkworm Pupa oil was 29.45%.
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Extraction of Silkworm-Pupa Oil
Technology & Development of Chemical Industry, 2008Co-Authors: Jiang Yan-zhongAbstract:The extraction methods of silkworm-Pupa oil were introduced.Supercritical CO2 extraction method and traditional solvent extraction method was compared.The results showed that the quality of silkworm-Pupa oil by supercritical CO2 method was better.
