The Experts below are selected from a list of 19971 Experts worldwide ranked by ideXlab platform
Francisco Lakay - One of the best experts on this subject based on the ideXlab platform.
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comparative analysis of environmental dna extraction and Purification Methods from different humic acid rich soils
Journal of Applied Microbiology, 2007Co-Authors: Francisco Lakay, Alfred Botha, Bernard A PriorAbstract:AIM: To establish a rapid, improved soil environmental DNA extraction and Purification protocol. Methods AND RESULTS: Three different soil DNA isolation and four Purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction Methods. The inclusion of soil washing prior to cell lysis decreased the amount of Purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful Purification. CONCLUSIONS: Optimization of a Purification protocol confirmed that only a combination of previously described Methods proved sufficient in yielding pure environmental DNA from humic-rich soils. Total processing time for DNA extraction and subsequent Purification from multiple samples was considerably more rapid than the previously described Methods. SIGNIFICANCE AND IMPACT OF THE STUDY: This study developed a new optimized soil DNA extraction and Purification protocol that is suitable for different environmental sources that are rich in humic acid content.
Jeanpierre Halle - One of the best experts on this subject based on the ideXlab platform.
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evaluation of alginate Purification Methods effect on polyphenol endotoxin and protein contamination
Journal of Biomedical Materials Research Part A, 2006Co-Authors: Julie Dusseault, Martin Menard, Stefania Polizu, Guillaume Jourdan, Lhocine Yahia, Jeanpierre HalleAbstract:Alginate, a polysaccharide extracted from brown seaweed, is widely used for the microencapsulation of islets of Langerhans, allowing their transplantation without immunosuppression. This natural polymer is known to be largely contaminated. The implantation of islets encapsulated using unpurified alginate leads to the development of fibrotic cell overgrowth around the microcapsules and normalization of the blood glucose is restricted to a very short period if it is achieved at all. Several research groups have developed their own Purification method and obtained relatively good results. No comparative evaluation of the efficiencies of these Methods has been published. We conducted an evaluative study of five different alginate preparations: a pharmaceutical-grade alginate in its raw state, the same alginate after Purification according to three different published Methods, and a commercially available purified alginate. The results showed that all Purification Methods reduced the amounts of known contaminants, that is, polyphenols, endotoxins, and proteins, although with varying efficiencies. Increased viscosity of alginate solutions was observed after Purification of the alginates. Despite a general efficiency in decreasing contamination levels, all of the purified alginates contained relatively high residual amounts of protein contaminants. Because proteins may be immunogenic, these residual proteins may have a role in persisting microcapsule immunogenicity.
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Evaluation of alginate Purification Methods: Effect on polyphenol, endotoxin, and protein contamination
Journal of Biomedical Materials Research Part A, 2006Co-Authors: Julie Dusseault, Martin Menard, Stefania Polizu, Guillaume Jourdan, Lhocine Yahia, Jeanpierre HalleAbstract:Alginate, a polysaccharide extracted from brown seaweed, is widely used for the microencapsulation of islets of Langerhans, allowing their transplantation without immunosuppression. This natural polymer is known to be largely contaminated. The implantation of islets encapsulated using unpurified alginate leads to the development of fibrotic cell overgrowth around the microcapsules and normalization of the blood glucose is restricted to a very short period if it is achieved at all. Several research groups have developed their own Purification method and obtained relatively good results. No comparative evaluation of the efficiencies of these Methods has been published. We conducted an evaluative study of five different alginate preparations: a pharmaceutical-grade alginate in its raw state, the same alginate after Purification according to three different published Methods, and a commercially available purified alginate. The results showed that all Purification Methods reduced the amounts of known contaminants, that is, polyphenols, endotoxins, and proteins, although with varying efficiencies. Increased viscosity of alginate solutions was observed after Purification of the alginates. Despite a general efficiency in decreasing contamination levels, all of the purified alginates contained relatively high residual amounts of protein contaminants. Because proteins may be immunogenic, these residual proteins may have a role in persisting microcapsule immunogenicity. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Bernard A Prior - One of the best experts on this subject based on the ideXlab platform.
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comparative analysis of environmental dna extraction and Purification Methods from different humic acid rich soils
Journal of Applied Microbiology, 2007Co-Authors: Francisco Lakay, Alfred Botha, Bernard A PriorAbstract:AIM: To establish a rapid, improved soil environmental DNA extraction and Purification protocol. Methods AND RESULTS: Three different soil DNA isolation and four Purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction Methods. The inclusion of soil washing prior to cell lysis decreased the amount of Purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful Purification. CONCLUSIONS: Optimization of a Purification protocol confirmed that only a combination of previously described Methods proved sufficient in yielding pure environmental DNA from humic-rich soils. Total processing time for DNA extraction and subsequent Purification from multiple samples was considerably more rapid than the previously described Methods. SIGNIFICANCE AND IMPACT OF THE STUDY: This study developed a new optimized soil DNA extraction and Purification protocol that is suitable for different environmental sources that are rich in humic acid content.
S. V. Sudareva - One of the best experts on this subject based on the ideXlab platform.
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Modern Methods for removing carbon dioxide from flue gases emitted by thermal power stations
Thermal Engineering, 2009Co-Authors: N. N. Ezhova, S. V. SudarevaAbstract:Modern Methods for removing carbon dioxide from flue gases emitted by power installations are briefly reviewed. Along with processes introduced at present at foreign power plants, new Purification Methods, the development of which is in progress, are discussed.
A. N. Kazakov - One of the best experts on this subject based on the ideXlab platform.
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Prospective technologies for using biohydrogen in power installations on the basis of fuel cells (a review)
Thermal Engineering, 2013Co-Authors: D. O. Dunikov, V. I. Borzenko, S. P. Malyshenko, D. V. Blinov, A. N. KazakovAbstract:The present state of technology for obtaining hydrogen by biological Methods and for purifying it is reviewed from the viewpoint of its possible use in kilowatt-class power installations. Hybrid membranesorption biohydrogen Purification Methods combining membrane-based pretreatment and sorption-based final treatment, also with the use of metal hydrides, should be regarded as the most efficient ones.
