The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Christine Amourette - One of the best experts on this subject based on the ideXlab platform.
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gulf war illness effects of repeated stress and Pyridostigmine treatment on blood brain barrier permeability and cholinesterase activity in rat brain
Behavioural Brain Research, 2009Co-Authors: Christine Amourette, Ioannis Lamproglou, Laure Barbier, William Fauquette, Amelie Zoppe, Roselyne Viret, Michel DiserboAbstract:Abstract After the first Persian Gulf War, many soldiers have complained of a variety of symptoms designated as “Gulf War Illness”. Among several factors, implication of Pyridostigmine (PB) in late cognitive dysfunction is highly likely. As a hypothesis to explain these behavioural disorders is a potentiation of the operational stress effects by Pyridostigmine. We have previously described that repeated stress combined to Pyridostigmine treatment induces learning dysfunction linked to genomic cerebral modifications [Barbier L, Diserbo M, Lamproglou I, Amourette C, Peinnequin A, Fauquette W. Repeated stress in combination with Pyridostigmine: part II—changes in selected cerebral genes expression. Behav Brain Res 2009;197:292–300; Lamproglou I, Barbier L, Diserbo M, Fauvelle F, Fauquette W, Amourette C. Repeated stress in combination with Pyridostigmine: part I—long-term behavioural consequences. Behav Brain Res 2009;197:301–10]. In the present study, using the same experimental model, we attempted to determine if such modifications are linked to a central passage of Pyridostigmine under stress. Indeed it is known that exposure to stress can disrupt blood–brain barrier (BBB) and thereby increase the neurotoxicity induced by chemicals in many cerebral areas. Adult rats were subjected to repeated stress based on a modification of the pole climbing avoidance technique and treated daily by PB (1.5 mg/kg/day, oral in water), for two 5-day periods separated by 2-day rest. Just after the last stress session, 3 H-Pyridostigmine was administered as a tracer to evaluate BBB breakdown. In brain micro-punches and brain coronal cryosections, we failed to detect any radioactivity in animals chronically stressed and treated by Pyridostigmine. Accordingly, no change of ChE activity was noted in any brain area studied. It thus appears that, in our experimental model, Pyridostigmine induces effects on central nervous system, but these effects do not seem to be mediated by a central passage of Pyridostigmine linked to a BBB opening under stress. These results suggest that Pyridostigmine may have central effects, under stress, via indirect mechanisms emerging from a peripheral pathway.
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repeated stress in combination with Pyridostigmine part i long term behavioural consequences
Behavioural Brain Research, 2009Co-Authors: Ioannis Lamproglou, Laure Barbier, William Fauquette, Michel Diserbo, Florence Fauvelle, Christine AmouretteAbstract:Since their return from the first Persian Gulf War, some veterans have complained of a variety of symptoms that were designated as "Gulf War Illness" (GWI). Among other factors, Pyridostigmine, used as a prophylaxis treatment against intoxication by nerve agents, has been proposed by many authors as a cause of late social and/or cognitive dysfunction related to GWI. One of the hypotheses placed to explain these behavioural disorders is that operational stress has modified the side effects of Pyridostigmine given to soldiers. In an attempt to establish an experimental model of GWI to evaluate the long-term behavioural effects of Pyridostigmine administered in stressful conditions, we have developed a new model of repeated stress based on the pole-climbing avoidance technique. We used it to evaluate the effects of Pyridostigmine treatment combined to repeated stress over the months following the end of the treatment. We observed that this stress induces impulsiveness and aggressiveness in adult male rat. Moreover, Pyridostigmine treatment administered daily 30 min before each stressful session amplifies these behavioural disorders and induces long-term learning dysfunction and slight but significant decrease in phosphocholine level in hippocampus. This suggests that repeated administration of Pyridostigmine combined to pole-climbing avoidance (PCA) stress conditions can induce adverse effects in rat central nervous system.
Michel Diserbo - One of the best experts on this subject based on the ideXlab platform.
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gulf war illness effects of repeated stress and Pyridostigmine treatment on blood brain barrier permeability and cholinesterase activity in rat brain
Behavioural Brain Research, 2009Co-Authors: Christine Amourette, Ioannis Lamproglou, Laure Barbier, William Fauquette, Amelie Zoppe, Roselyne Viret, Michel DiserboAbstract:Abstract After the first Persian Gulf War, many soldiers have complained of a variety of symptoms designated as “Gulf War Illness”. Among several factors, implication of Pyridostigmine (PB) in late cognitive dysfunction is highly likely. As a hypothesis to explain these behavioural disorders is a potentiation of the operational stress effects by Pyridostigmine. We have previously described that repeated stress combined to Pyridostigmine treatment induces learning dysfunction linked to genomic cerebral modifications [Barbier L, Diserbo M, Lamproglou I, Amourette C, Peinnequin A, Fauquette W. Repeated stress in combination with Pyridostigmine: part II—changes in selected cerebral genes expression. Behav Brain Res 2009;197:292–300; Lamproglou I, Barbier L, Diserbo M, Fauvelle F, Fauquette W, Amourette C. Repeated stress in combination with Pyridostigmine: part I—long-term behavioural consequences. Behav Brain Res 2009;197:301–10]. In the present study, using the same experimental model, we attempted to determine if such modifications are linked to a central passage of Pyridostigmine under stress. Indeed it is known that exposure to stress can disrupt blood–brain barrier (BBB) and thereby increase the neurotoxicity induced by chemicals in many cerebral areas. Adult rats were subjected to repeated stress based on a modification of the pole climbing avoidance technique and treated daily by PB (1.5 mg/kg/day, oral in water), for two 5-day periods separated by 2-day rest. Just after the last stress session, 3 H-Pyridostigmine was administered as a tracer to evaluate BBB breakdown. In brain micro-punches and brain coronal cryosections, we failed to detect any radioactivity in animals chronically stressed and treated by Pyridostigmine. Accordingly, no change of ChE activity was noted in any brain area studied. It thus appears that, in our experimental model, Pyridostigmine induces effects on central nervous system, but these effects do not seem to be mediated by a central passage of Pyridostigmine linked to a BBB opening under stress. These results suggest that Pyridostigmine may have central effects, under stress, via indirect mechanisms emerging from a peripheral pathway.
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repeated stress in combination with Pyridostigmine part i long term behavioural consequences
Behavioural Brain Research, 2009Co-Authors: Ioannis Lamproglou, Laure Barbier, William Fauquette, Michel Diserbo, Florence Fauvelle, Christine AmouretteAbstract:Since their return from the first Persian Gulf War, some veterans have complained of a variety of symptoms that were designated as "Gulf War Illness" (GWI). Among other factors, Pyridostigmine, used as a prophylaxis treatment against intoxication by nerve agents, has been proposed by many authors as a cause of late social and/or cognitive dysfunction related to GWI. One of the hypotheses placed to explain these behavioural disorders is that operational stress has modified the side effects of Pyridostigmine given to soldiers. In an attempt to establish an experimental model of GWI to evaluate the long-term behavioural effects of Pyridostigmine administered in stressful conditions, we have developed a new model of repeated stress based on the pole-climbing avoidance technique. We used it to evaluate the effects of Pyridostigmine treatment combined to repeated stress over the months following the end of the treatment. We observed that this stress induces impulsiveness and aggressiveness in adult male rat. Moreover, Pyridostigmine treatment administered daily 30 min before each stressful session amplifies these behavioural disorders and induces long-term learning dysfunction and slight but significant decrease in phosphocholine level in hippocampus. This suggests that repeated administration of Pyridostigmine combined to pole-climbing avoidance (PCA) stress conditions can induce adverse effects in rat central nervous system.
Jihyun An - One of the best experts on this subject based on the ideXlab platform.
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neuromuscular blockade reversal with sugammadex versus Pyridostigmine glycopyrrolate in laparoscopic cholecystectomy a randomized trial of effects on postoperative gastrointestinal motility
Korean Journal of Anesthesiology, 2020Co-Authors: Jihyun AnAbstract:Background Acetylcholinesterase inhibitors (e.g., Pyridostigmine bromide) are used for neuromuscular blockade (NMB) reversal in patients undergoing surgery under general anesthesia (GA). Concurrent use of anticholinergic agents (e.g., glycopyrrolate) decreases cholinergic side effects but can impede bowel movements. Sugammadex has no cholinergic effects; its use modifies recovery of gastrointestinal (GI) motility following laparoscopic cholecystectomy compared to Pyridostigmine/glycopyrrolate. This study evaluated the contribution of sugammadex to the recovery of GI motility compared with Pyridostigmine and glycopyrrolate. Methods We conducted a prospective study of patients who underwent laparoscopic cholecystectomy. Patients were randomly allocated to the experimental group (sugammadex, Group S) or control group (Pyridostigmine-glycopyrrolate, Group P). After anesthesia (propofol and rocuronium, and 2% sevoflurane), recovery was induced by injection of sugammadex or a Pyridostigmine-glycopyrrolate mixture. As a primary outcome, patients recorded the time of their first passage of flatus ('gas-out time') and defecation. The secondary outcome was stool types. Results One-hundred and two patients participated (Group S, 49; Group P, 53). Mean time from injection of NMB reversal agents to gas-out time was 15.03 (6.36-20.25) h in Group S and 20.85 (16.34-25.86) h in Group P (P = 0.001). Inter-group differences were significant. Time until the first defecation as well as types of stools was not significantly different. Conclusions Sugammadex after laparoscopic cholecystectomy under GA resulted in an earlier first postoperative passage of flatus compared with the use of a mixture of Pyridostigmine and glycopyrrolate. These findings suggest that the use of sugammadex has positive effects on the recovery of GI motility.
Ioannis Lamproglou - One of the best experts on this subject based on the ideXlab platform.
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gulf war illness effects of repeated stress and Pyridostigmine treatment on blood brain barrier permeability and cholinesterase activity in rat brain
Behavioural Brain Research, 2009Co-Authors: Christine Amourette, Ioannis Lamproglou, Laure Barbier, William Fauquette, Amelie Zoppe, Roselyne Viret, Michel DiserboAbstract:Abstract After the first Persian Gulf War, many soldiers have complained of a variety of symptoms designated as “Gulf War Illness”. Among several factors, implication of Pyridostigmine (PB) in late cognitive dysfunction is highly likely. As a hypothesis to explain these behavioural disorders is a potentiation of the operational stress effects by Pyridostigmine. We have previously described that repeated stress combined to Pyridostigmine treatment induces learning dysfunction linked to genomic cerebral modifications [Barbier L, Diserbo M, Lamproglou I, Amourette C, Peinnequin A, Fauquette W. Repeated stress in combination with Pyridostigmine: part II—changes in selected cerebral genes expression. Behav Brain Res 2009;197:292–300; Lamproglou I, Barbier L, Diserbo M, Fauvelle F, Fauquette W, Amourette C. Repeated stress in combination with Pyridostigmine: part I—long-term behavioural consequences. Behav Brain Res 2009;197:301–10]. In the present study, using the same experimental model, we attempted to determine if such modifications are linked to a central passage of Pyridostigmine under stress. Indeed it is known that exposure to stress can disrupt blood–brain barrier (BBB) and thereby increase the neurotoxicity induced by chemicals in many cerebral areas. Adult rats were subjected to repeated stress based on a modification of the pole climbing avoidance technique and treated daily by PB (1.5 mg/kg/day, oral in water), for two 5-day periods separated by 2-day rest. Just after the last stress session, 3 H-Pyridostigmine was administered as a tracer to evaluate BBB breakdown. In brain micro-punches and brain coronal cryosections, we failed to detect any radioactivity in animals chronically stressed and treated by Pyridostigmine. Accordingly, no change of ChE activity was noted in any brain area studied. It thus appears that, in our experimental model, Pyridostigmine induces effects on central nervous system, but these effects do not seem to be mediated by a central passage of Pyridostigmine linked to a BBB opening under stress. These results suggest that Pyridostigmine may have central effects, under stress, via indirect mechanisms emerging from a peripheral pathway.
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repeated stress in combination with Pyridostigmine part i long term behavioural consequences
Behavioural Brain Research, 2009Co-Authors: Ioannis Lamproglou, Laure Barbier, William Fauquette, Michel Diserbo, Florence Fauvelle, Christine AmouretteAbstract:Since their return from the first Persian Gulf War, some veterans have complained of a variety of symptoms that were designated as "Gulf War Illness" (GWI). Among other factors, Pyridostigmine, used as a prophylaxis treatment against intoxication by nerve agents, has been proposed by many authors as a cause of late social and/or cognitive dysfunction related to GWI. One of the hypotheses placed to explain these behavioural disorders is that operational stress has modified the side effects of Pyridostigmine given to soldiers. In an attempt to establish an experimental model of GWI to evaluate the long-term behavioural effects of Pyridostigmine administered in stressful conditions, we have developed a new model of repeated stress based on the pole-climbing avoidance technique. We used it to evaluate the effects of Pyridostigmine treatment combined to repeated stress over the months following the end of the treatment. We observed that this stress induces impulsiveness and aggressiveness in adult male rat. Moreover, Pyridostigmine treatment administered daily 30 min before each stressful session amplifies these behavioural disorders and induces long-term learning dysfunction and slight but significant decrease in phosphocholine level in hippocampus. This suggests that repeated administration of Pyridostigmine combined to pole-climbing avoidance (PCA) stress conditions can induce adverse effects in rat central nervous system.
Laure Barbier - One of the best experts on this subject based on the ideXlab platform.
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gulf war illness effects of repeated stress and Pyridostigmine treatment on blood brain barrier permeability and cholinesterase activity in rat brain
Behavioural Brain Research, 2009Co-Authors: Christine Amourette, Ioannis Lamproglou, Laure Barbier, William Fauquette, Amelie Zoppe, Roselyne Viret, Michel DiserboAbstract:Abstract After the first Persian Gulf War, many soldiers have complained of a variety of symptoms designated as “Gulf War Illness”. Among several factors, implication of Pyridostigmine (PB) in late cognitive dysfunction is highly likely. As a hypothesis to explain these behavioural disorders is a potentiation of the operational stress effects by Pyridostigmine. We have previously described that repeated stress combined to Pyridostigmine treatment induces learning dysfunction linked to genomic cerebral modifications [Barbier L, Diserbo M, Lamproglou I, Amourette C, Peinnequin A, Fauquette W. Repeated stress in combination with Pyridostigmine: part II—changes in selected cerebral genes expression. Behav Brain Res 2009;197:292–300; Lamproglou I, Barbier L, Diserbo M, Fauvelle F, Fauquette W, Amourette C. Repeated stress in combination with Pyridostigmine: part I—long-term behavioural consequences. Behav Brain Res 2009;197:301–10]. In the present study, using the same experimental model, we attempted to determine if such modifications are linked to a central passage of Pyridostigmine under stress. Indeed it is known that exposure to stress can disrupt blood–brain barrier (BBB) and thereby increase the neurotoxicity induced by chemicals in many cerebral areas. Adult rats were subjected to repeated stress based on a modification of the pole climbing avoidance technique and treated daily by PB (1.5 mg/kg/day, oral in water), for two 5-day periods separated by 2-day rest. Just after the last stress session, 3 H-Pyridostigmine was administered as a tracer to evaluate BBB breakdown. In brain micro-punches and brain coronal cryosections, we failed to detect any radioactivity in animals chronically stressed and treated by Pyridostigmine. Accordingly, no change of ChE activity was noted in any brain area studied. It thus appears that, in our experimental model, Pyridostigmine induces effects on central nervous system, but these effects do not seem to be mediated by a central passage of Pyridostigmine linked to a BBB opening under stress. These results suggest that Pyridostigmine may have central effects, under stress, via indirect mechanisms emerging from a peripheral pathway.
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repeated stress in combination with Pyridostigmine part i long term behavioural consequences
Behavioural Brain Research, 2009Co-Authors: Ioannis Lamproglou, Laure Barbier, William Fauquette, Michel Diserbo, Florence Fauvelle, Christine AmouretteAbstract:Since their return from the first Persian Gulf War, some veterans have complained of a variety of symptoms that were designated as "Gulf War Illness" (GWI). Among other factors, Pyridostigmine, used as a prophylaxis treatment against intoxication by nerve agents, has been proposed by many authors as a cause of late social and/or cognitive dysfunction related to GWI. One of the hypotheses placed to explain these behavioural disorders is that operational stress has modified the side effects of Pyridostigmine given to soldiers. In an attempt to establish an experimental model of GWI to evaluate the long-term behavioural effects of Pyridostigmine administered in stressful conditions, we have developed a new model of repeated stress based on the pole-climbing avoidance technique. We used it to evaluate the effects of Pyridostigmine treatment combined to repeated stress over the months following the end of the treatment. We observed that this stress induces impulsiveness and aggressiveness in adult male rat. Moreover, Pyridostigmine treatment administered daily 30 min before each stressful session amplifies these behavioural disorders and induces long-term learning dysfunction and slight but significant decrease in phosphocholine level in hippocampus. This suggests that repeated administration of Pyridostigmine combined to pole-climbing avoidance (PCA) stress conditions can induce adverse effects in rat central nervous system.
