The Experts below are selected from a list of 70458 Experts worldwide ranked by ideXlab platform
Xiaoyuan Zhu - One of the best experts on this subject based on the ideXlab platform.
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease
Elsevier, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future. Keywords: QTL mapping, Rice, Rice false smut disease, Ustilaginoidea viren
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease.
Current Plant Biology, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Abstract Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future.
Mebeaselassie Andargie - One of the best experts on this subject based on the ideXlab platform.
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease
Elsevier, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future. Keywords: QTL mapping, Rice, Rice false smut disease, Ustilaginoidea viren
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease.
Current Plant Biology, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Abstract Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future.
Masahiro Yano - One of the best experts on this subject based on the ideXlab platform.
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fine mapping of rbg2 a Quantitative Trait Locus for resistance to burkholderia glumae on rice chromosome 1
Molecular Breeding, 2015Co-Authors: Ritsuko Mizobuchi, Seiya Tsushima, Shuichi Fukuoka, Hiroyuki Sato, Masahiro YanoAbstract:Bacterial grain rot (BGR), caused by the bacterial pathogen Burkholderia glumae, is a destructive disease of rice. At anthesis, rice panicles are attacked by the pathogen, and the infection causes unfilled or aborted grains, reducing grain yield and quality. Thus, increasing the level of BGR resistance is an important objective for rice breeding. A Quantitative Trait Locus (QTL) on rice chromosome 1 that controls BGR resistance was previously detected in backcross inbred lines (BILs) derived from a cross between Kele, a resistant traditional lowland cultivar (indica) that originated in India, and Hitomebore, a susceptible modern lowland cultivar (temperate japonica) from Japan. Further genetic analyses using a BC3F6 population derived from a cross between a resistant BIL (BC2F5) and Hitomebore confirmed that a QTL for BGR resistance was located on the long arm of chromosome 1. To define more precisely the chromosomal region underlying this QTL, we identified nine BC2F6 plants in which recombination occurred near the QTL. Substitution mapping using homozygous recombinant and nonrecombinant plants demonstrated that the QTL, here designated as Resistance to Burkholderia glumae 2 (RBG2), was located in a 502-kb interval defined by simple sequence repeat markers RM1216 and RM11727.
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fine mapping of sta1 a Quantitative Trait Locus determining stele transversal area on rice chromosome 9
Molecular Breeding, 2010Co-Authors: Yusaku Uga, Kazutoshi Okuno, Masahiro YanoAbstract:The stele (root vascular cylinder) in plants plays an important role in the transport of water and nutrients from the root to the shoot. A Quantitative Trait Locus (QTL) on rice chromosome 9 that controls stele transversal area (STA) was previously detected in an F3 mapping population derived from a cross between the lowland cultivar ‘IR64’, with a small STA, and the upland cultivar ‘Kinandang Patong’, with a large STA. To identify the gene(s) underlying this QTL, we undertook fine mapping of the Locus. We screened eight plants from BC2F3 lines in which recombination occurred near the QTL. Progeny testing of BC2F4 plants was used to determine the genotype classes for the QTL in each BC2F3 line. Accordingly, the STA QTL Sta1 (Stele Transversal Area 1) was mapped between the InDel markers ID07_12 and ID07_14. A candidate genomic region for Sta1 was defined more precisely between markers RM566 and RM24334, which delimit a 359-kb interval in the reference cultivar ‘Nipponbare’. A line homozygous for the ‘Kinandang Patong’ allele of Sta1 had an STA approximately 28.4% larger than that of ‘IR64’. However, Sta1 did not influence maximum or total root length, suggesting that this QTL specifically controls STA.
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a major Quantitative Trait Locus controlling cadmium translocation in rice oryza sativa
New Phytologist, 2009Co-Authors: Daisei Ueno, Masahiro Yano, Izumi Kono, Kengo Yokosho, T Ando, Jian FengAbstract:Summary • The Trait of low cadmium (Cd) accumulation in brown rice (Oryza sativa) is important for food safety. An effective way to reduce Cd accumulation in the grain is to control Cd transfer from the roots to the shoots. Here, we investigated genotypic variation in the shoot Cd concentration among 146 accessions from a rice core collection and performed a Quantitative Trait Locus (QTL) analysis to determine the loci controlling shoot Cd accumulation. Furthermore, we physiologically characterized the two accessions used for QTL analysis. Large genotypic variation (13-fold) in the shoot Cd concentration was found. A major QTL was detected on chromosome 11 using a F 2 population derived from Badari Dhan (a high-Cd accession) and Shwe War (a low-Cd accession). This QTL explained 16.1% of the phenotypic variation in Cd accumulation. Furthermore, this QTL was confirmed by analysis of advanced progeny. Physiological studies showed that Badari Dhan and Shwe War did not differ in uptake of Cd by the roots, but differed greatly in the translocation of Cd from the roots to the shoots. Taken together, our findings suggest that the major QTL detected is responsible for the translocation of Cd from the roots to the shoots. Authors for correspondence:
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hd6 a rice Quantitative Trait Locus involved in photoperiod sensitivity encodes the α subunit of protein kinase ck2
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Yuji Takahashi, Ayahiko Shomura, Takuji Sasaki, Masahiro YanoAbstract:Hd6 is a Quantitative Trait Locus involved in rice photoperiod sensitivity. It was detected in backcross progeny derived from a cross between the japonica variety Nipponbare and the indica variety Kasalath. To isolate a gene at Hd6, we used a large segregating population for the high-resolution and fine-scale mapping of Hd6 and constructed genomic clone contigs around the Hd6 region. Linkage analysis with P1-derived artificial chromosome clone-derived DNA markers delimited Hd6 to a 26.4-kb genomic region. We identified a gene encoding the α subunit of protein kinase CK2 (CK2α) in this region. The Nipponbare allele of CK2α contains a premature stop codon, and the resulting truncated product is undoubtedly nonfunctional. Genetic complementation analysis revealed that the Kasalath allele of CK2α increases days-to-heading. Map-based cloning with advanced backcross progeny enabled us to identify a gene underlying a Quantitative Trait Locus even though it exhibited a relatively small effect on the phenotype.
Craig G Yencho - One of the best experts on this subject based on the ideXlab platform.
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Quantitative Trait Locus mapping for common scab resistance in a tetraploid potato full sib population
Plant Disease, 2021Co-Authors: Guilherme Da Silva Pereira, Kathleen G. Haynes, Marcelo Mollinari, Christian A Thill, Zhaobang Zeng, Craig G YenchoAbstract:Despite the negative impact of common scab (Streptomyces spp.) on the potato industry, little is known about the genetic architecture of resistance to this bacterial disease in the crop. We evaluated a mapping population (~150 full-sibs) derived from a cross between two tetraploid potatoes ('Atlantic' × B1829-5) in three environments (MN11, PA11, ME12) under natural common scab pressure. Three measures to common scab reaction, namely percentage of scabby tubers, and disease area and lesion indices, were found to be highly correlated (>0.76). Due to the large environmental effect, heritability values were zero for all three Traits in MN11, but moderate to high in PA11 and ME12 (0.44~0.79). We identified a single Quantitative Trait Locus (QTL) for lesion index in PA11, ME12 and joint analyses on linkage group 3, explaining 22~30% of the total variation. The identification of QTL haplotypes and candidate genes contributing to disease resistance can support genomics-assisted breeding approaches in the crop.
Aiqing Feng - One of the best experts on this subject based on the ideXlab platform.
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease
Elsevier, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future. Keywords: QTL mapping, Rice, Rice false smut disease, Ustilaginoidea viren
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Mapping of the Quantitative Trait Locus (QTL) conferring resistance to rice false smut disease.
Current Plant Biology, 2018Co-Authors: Mebeaselassie Andargie, Aiqing Feng, Xiaoyuan ZhuAbstract:Abstract Rice false smut disease, caused by Ustilaginoidea virens, is an important grain disease in rice production worldwide. An F2 population of 176 plants was used to conduct Quantitative Trait Locus (QTL) mapping for rice false smut disease resistance. A total of 360 simple sequence repeat (SSR) markers were screened for polymorphism on the parents, susceptible and resistant bulks, which yielded SSR markers linked to false smut disease resistance gene. Bulk segregant analysis located the resistance gene to chromosome 5. QTL analysis also identified two QTLs on chromosome 5, the phenotypic variations explained by these two QTLs are 7.3% and 16.4%. The qRFSr-5.1 was about 0.8 centimorgans (cM) to SSR marker RM18451 accounting for 7.3% of phenotypic variation, and qRFSr-5.2 about 1.1 cM to SSR marker M72 accounting for 16.4%. The SSR markers linked to rice false smut disease resistance genes can be used for marker-assisted selection and also they serve as landmarks for cloning false smut disease resistance genes in the future.
