Racemic Mixture

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Rong Xiao - One of the best experts on this subject based on the ideXlab platform.

  • In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Yaohui Li, Xiaotian Zhou, Hongbo Liang, Yan Xu, Jiawei Jiang, Rong Xiao, Lei Wang, Ye Ni
    Abstract:

    BackgroundCandida parapsilosis ( R )-carbonyl reductase (RCR) and ( S )-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic ( R,S )-1-phenyl-1,2-ethanediol (PED) to its ( S )-isomer. RCR catalyzes ( R )-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to ( S )-PED. However, the stereoconversion efficiency of Racemic Mixture to ( S )-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to ( S )-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways.ResultsAn in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis . With respect to wild-type, recombinant C. parapsilosis /pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic ( R,S )-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to ( S )-PED. The ratio of k _ cat / K _ M for SCR catalyzing ( R )-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to ( S )-PED. Using these strategies, the recombinant C. parapsilosis /pCP-RCR catalyzed Racemic PED to its ( S )-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h.ConclusionsWe established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

  • in situ expression of r carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to s phenyl 1 2 ethanediol in candida parapsilosis cctcc m203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Xiaotian Zhou, Hongbo Liang, Jiawei Jiang, Lei Wang, Rong Xiao
    Abstract:

    Candida parapsilosis (R)-carbonyl reductase (RCR) and (S)-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic (R,S)-1-phenyl-1,2-ethanediol (PED) to its (S)-isomer. RCR catalyzes (R)-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to (S)-PED. However, the stereoconversion efficiency of Racemic Mixture to (S)-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to (S)-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways. An in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis. With respect to wild-type, recombinant C. parapsilosis/pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic (R,S)-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to (S)-PED. The ratio of k cat /K M for SCR catalyzing (R)-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to (S)-PED. Using these strategies, the recombinant C. parapsilosis/pCP-RCR catalyzed Racemic PED to its (S)-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h. We established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

Rongzhen Zhang - One of the best experts on this subject based on the ideXlab platform.

  • In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Yaohui Li, Xiaotian Zhou, Hongbo Liang, Yan Xu, Jiawei Jiang, Rong Xiao, Lei Wang, Ye Ni
    Abstract:

    BackgroundCandida parapsilosis ( R )-carbonyl reductase (RCR) and ( S )-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic ( R,S )-1-phenyl-1,2-ethanediol (PED) to its ( S )-isomer. RCR catalyzes ( R )-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to ( S )-PED. However, the stereoconversion efficiency of Racemic Mixture to ( S )-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to ( S )-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways.ResultsAn in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis . With respect to wild-type, recombinant C. parapsilosis /pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic ( R,S )-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to ( S )-PED. The ratio of k _ cat / K _ M for SCR catalyzing ( R )-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to ( S )-PED. Using these strategies, the recombinant C. parapsilosis /pCP-RCR catalyzed Racemic PED to its ( S )-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h.ConclusionsWe established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

  • in situ expression of r carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to s phenyl 1 2 ethanediol in candida parapsilosis cctcc m203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Xiaotian Zhou, Hongbo Liang, Jiawei Jiang, Lei Wang, Rong Xiao
    Abstract:

    Candida parapsilosis (R)-carbonyl reductase (RCR) and (S)-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic (R,S)-1-phenyl-1,2-ethanediol (PED) to its (S)-isomer. RCR catalyzes (R)-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to (S)-PED. However, the stereoconversion efficiency of Racemic Mixture to (S)-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to (S)-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways. An in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis. With respect to wild-type, recombinant C. parapsilosis/pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic (R,S)-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to (S)-PED. The ratio of k cat /K M for SCR catalyzing (R)-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to (S)-PED. Using these strategies, the recombinant C. parapsilosis/pCP-RCR catalyzed Racemic PED to its (S)-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h. We established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

Ye Ni - One of the best experts on this subject based on the ideXlab platform.

  • In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Yaohui Li, Xiaotian Zhou, Hongbo Liang, Yan Xu, Jiawei Jiang, Rong Xiao, Lei Wang, Ye Ni
    Abstract:

    BackgroundCandida parapsilosis ( R )-carbonyl reductase (RCR) and ( S )-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic ( R,S )-1-phenyl-1,2-ethanediol (PED) to its ( S )-isomer. RCR catalyzes ( R )-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to ( S )-PED. However, the stereoconversion efficiency of Racemic Mixture to ( S )-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to ( S )-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways.ResultsAn in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis . With respect to wild-type, recombinant C. parapsilosis /pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic ( R,S )-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to ( S )-PED. The ratio of k _ cat / K _ M for SCR catalyzing ( R )-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to ( S )-PED. Using these strategies, the recombinant C. parapsilosis /pCP-RCR catalyzed Racemic PED to its ( S )-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h.ConclusionsWe established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

Jiawei Jiang - One of the best experts on this subject based on the ideXlab platform.

  • In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Yaohui Li, Xiaotian Zhou, Hongbo Liang, Yan Xu, Jiawei Jiang, Rong Xiao, Lei Wang, Ye Ni
    Abstract:

    BackgroundCandida parapsilosis ( R )-carbonyl reductase (RCR) and ( S )-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic ( R,S )-1-phenyl-1,2-ethanediol (PED) to its ( S )-isomer. RCR catalyzes ( R )-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to ( S )-PED. However, the stereoconversion efficiency of Racemic Mixture to ( S )-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to ( S )-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways.ResultsAn in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis . With respect to wild-type, recombinant C. parapsilosis /pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic ( R,S )-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to ( S )-PED. The ratio of k _ cat / K _ M for SCR catalyzing ( R )-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to ( S )-PED. Using these strategies, the recombinant C. parapsilosis /pCP-RCR catalyzed Racemic PED to its ( S )-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h.ConclusionsWe established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

  • in situ expression of r carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to s phenyl 1 2 ethanediol in candida parapsilosis cctcc m203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Xiaotian Zhou, Hongbo Liang, Jiawei Jiang, Lei Wang, Rong Xiao
    Abstract:

    Candida parapsilosis (R)-carbonyl reductase (RCR) and (S)-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic (R,S)-1-phenyl-1,2-ethanediol (PED) to its (S)-isomer. RCR catalyzes (R)-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to (S)-PED. However, the stereoconversion efficiency of Racemic Mixture to (S)-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to (S)-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways. An in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis. With respect to wild-type, recombinant C. parapsilosis/pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic (R,S)-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to (S)-PED. The ratio of k cat /K M for SCR catalyzing (R)-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to (S)-PED. Using these strategies, the recombinant C. parapsilosis/pCP-RCR catalyzed Racemic PED to its (S)-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h. We established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

Lei Wang - One of the best experts on this subject based on the ideXlab platform.

  • In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Yaohui Li, Xiaotian Zhou, Hongbo Liang, Yan Xu, Jiawei Jiang, Rong Xiao, Lei Wang, Ye Ni
    Abstract:

    BackgroundCandida parapsilosis ( R )-carbonyl reductase (RCR) and ( S )-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic ( R,S )-1-phenyl-1,2-ethanediol (PED) to its ( S )-isomer. RCR catalyzes ( R )-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to ( S )-PED. However, the stereoconversion efficiency of Racemic Mixture to ( S )-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to ( S )-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways.ResultsAn in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis . With respect to wild-type, recombinant C. parapsilosis /pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic ( R,S )-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to ( S )-PED. The ratio of k _ cat / K _ M for SCR catalyzing ( R )-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to ( S )-PED. Using these strategies, the recombinant C. parapsilosis /pCP-RCR catalyzed Racemic PED to its ( S )-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h.ConclusionsWe established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.

  • in situ expression of r carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of Racemic Mixture to s phenyl 1 2 ethanediol in candida parapsilosis cctcc m203011
    Microbial Cell Factories, 2016
    Co-Authors: Rongzhen Zhang, Xiaotian Zhou, Hongbo Liang, Jiawei Jiang, Lei Wang, Rong Xiao
    Abstract:

    Candida parapsilosis (R)-carbonyl reductase (RCR) and (S)-carbonyl reductase (SCR) are involved in the stereoconversion of Racemic (R,S)-1-phenyl-1,2-ethanediol (PED) to its (S)-isomer. RCR catalyzes (R)-PED to 2-hydroxyacetophenone (2-HAP), and SCR catalyzes 2-HAP to (S)-PED. However, the stereoconversion efficiency of Racemic Mixture to (S)-PED is not high because of an activity imbalance between RCR and SCR, with RCR performing at a lower rate than SCR. To realize the efficient preparation of Racemic Mixture to (S)-PED, an in situ expression of RCR and a two-stage control strategy were introduced to rebalance the RCR- and SCR-mediated pathways. An in situ expression plasmid pCP was designed and RCR was successfully expressed in C. parapsilosis. With respect to wild-type, recombinant C. parapsilosis/pCP-RCR exhibited over four-fold higher activity for catalyzing Racemic (R,S)-PED to 2-HAP, while maintained the activity for catalyzing 2-HAP to (S)-PED. The ratio of k cat /K M for SCR catalyzing (R)-PED and RCR catalyzing 2-HAP was about 1.0, showing the good balance between the functions of SCR and RCR. Based on pH and temperature preferences of RCR and SCR, a two-stage control strategy was devised, where pH and temperature were initially set at 5.0 and 30 °C for RCR rapidly catalyzing Racemic PED to 2-HAP, and then adjusted to 4.5 and 35 °C for SCR transforming 2-HAP to (S)-PED. Using these strategies, the recombinant C. parapsilosis/pCP-RCR catalyzed Racemic PED to its (S)-isomer with an optical purity of 98.8 % and a yield of 48.4 %. Most notably, the biotransformation duration was reduced from 48 to 13 h. We established an in situ expression system for RCR in C. parapsilosis to rebalance the functions between RCR and SCR. Then we designed a two-stage control strategy based on pH and temperature preferences of RCR and SCR, better rebalancing RCR and SCR-mediated chiral biosynthesis pathways. This work demonstrates a method to improve chiral biosyntheses via in situ expression of rate-limiting enzyme and a multi-stage control strategy to rebalance asymmetric pathways.