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Helga Lotz - One of the best experts on this subject based on the ideXlab platform.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between rocket height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid fine needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 ± 11.9 g/l vs 1.35 ± 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between "rocket" height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid find needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 +/- 11.9 g/l vs. 1.35 +/- 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
G. B. Salabè - One of the best experts on this subject based on the ideXlab platform.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between rocket height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid fine needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 ± 11.9 g/l vs 1.35 ± 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between "rocket" height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid find needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 +/- 11.9 g/l vs. 1.35 +/- 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
Luciano Corvo - One of the best experts on this subject based on the ideXlab platform.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between rocket height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid fine needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 ± 11.9 g/l vs 1.35 ± 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
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thyroglobulin determined in thyroid fine needle aspiration biopsies by Radial Immunodiffusion and electroImmunodiffusion
Clinical Chemistry and Laboratory Medicine, 1996Co-Authors: G. B. Salabè, Luciano Corvo, Helga LotzAbstract:Radial Immunodiffusion and electroImmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by Radial Immunodiffusion (0.5-3.0 g/l), and between "rocket" height and thyroglobulin concentration by electroImmunodiffusion (0.1-2.0 g/l). A nearly complete correlation was observed between the two methods (r = 0.97). In Radial Immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/l. The intrathyroidal concentration of thyroglobulin was determined by Radial Immunodiffusion and the thyroid find needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory- and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 +/- 11.9 g/l vs. 1.35 +/- 0.8 g/l, p = 0.005). In conclusion Radial Immunodiffusion and electroImmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by Radial Immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.
J Howard - One of the best experts on this subject based on the ideXlab platform.
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comparison of igg concentrations by Radial Immunodiffusion electrophoretic gamma globulin concentrations and total globulins in neonatal foals
Equine Veterinary Journal, 2017Co-Authors: Lisa Tscheschlok, Monica Venner, J HowardAbstract:REASONS FOR PERFORMING STUDY: Failure of transfer of passive immunity (FTPI) in foals is associated with a risk of infection and death. The current diagnostic gold standard is quantification of immunoglobulins using Radial Immunodiffusion (IgG-RID). Routine diagnosis is often performed using semi-quantitative tests. Concentrations of serum electrophoretic gamma globulins (EGG) and total globulins may be useful to assess FTPI, but few studies have investigated their use. OBJECTIVES: To assess agreement between IgG-RID and EGG, and evaluate the accuracy of total globulin concentration to diagnose FTPI based on both IgG-RID and EGG. STUDY DESIGN: Prospective study. METHODS: 360 serum samples were harvested at 6-24 hours post natum from 60 German Warmblood foals. Concentrations of EGG, IgG-RID and total globulin concentration (calculated from total proteins and albumin) were measured. Agreement between EGG and IgG-RID was assessed using Bland-Altman plots and Passing-Bablok regression. The accuracy of total globulin concentration was assessed using rank correlation and ROC curve analysis. RESULTS: Good agreement was found with slightly lower EGG than IgG-RID concentrations (Bland-Altman systemic bias, -1.9 g/L) which was more pronounced at higher concentrations (regression equation: IgG-RID = -0.78 +1.28xEGG). Correlations between total globulin concentration and EGG, and total globulin concentration and IgG-RID were 0.93 and 0.79, respectively. The area under the curve was 0.982 and 0.952 for EGG 95% with 71.2% (IgG-RID) and 90.5% (EGG) specificity for 90% with 66.0% (IgG-RID) and 87.9% (EGG) specificity for <8 g/L. CONCLUSIONS: There is good agreement between EGG and IgG-RID, with slightly more conservative estimates of immunoglobulins obtained using EGG. Total globulins may be a useful and economic quantitative screening test with cut-offs achieving high sensitivities, but analyser-specific cut-offs may be necessary. This article is protected by copyright. All rights reserved. KEYWORDS: IgG; electrophoresis; foal; globulins; horse; Radial Immunodiffusion. This article is protected by copyright. All rights reserved.
J. M. Molleda - One of the best experts on this subject based on the ideXlab platform.
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Concentrations of plasma immunoglobulins in the dog as determined by laser nephelometry. Comparison with Radial Immunodiffusion and enzyme-linked immunosorbent assay
Clinical Chemistry and Laboratory Medicine, 1997Co-Authors: Pedro J. Ginel, Juan M. Margarito, R. Lucena, J. M. MolledaAbstract:Summary: Concentrations of immunoglobuli ns were determined by laser nephelometry and, for comparison, by single Radial Immunodiffusion assay and a sandwich enzyme-linked immunosorbent assay (ELISA) in plasma samples of normal dogs. Correlation between methods was high, although the mean IgM concentrations determined by laser nephelometry were significantly higher than those determined by the single Radial immunodirrusion assay or ELISA. In contrast, the mean IgA concentrations were significantly lower than those determined by ELISA. Laser nephelometry was the most reproducible method. The between-assay coefficient of variation was 4.12% for IgG, 6.98% for IgM and 6.35% for IgA. Laser nephelometry and ELISA showed similar accuracies, and both were more accurate than single Radial immunodirrusion. Finally, laser nephelometry was much more sensitive than single Radial immunodifrusion, but less sensitive than the ELISA method. In conclusion, results of the three methods were in general comparable. The higher range of linearity, precision and accuracy of laser nephelometry, and the availability of automated nephelometers make this the method of choice for quantifying canine plasma immunoglobulins.