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R.j. Warrington - One of the best experts on this subject based on the ideXlab platform.
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Lack of Correlation between Severity of Clinical Symptoms, Skin Test Reactivity, and Radioallergosorbent Test Results in Venom-Allergic Patients
Allergy Asthma & Clinical Immunology, 2006Co-Authors: R.j. WarringtonAbstract:Purpose To retrospectively examine the relation between skin Test reactivity, venom-specific immunoglobulin E (IgE) antibody levels, and severity of clinical reaction in patients with insect venom allergy. Method Thirty-six patients (including 15 females) who presented with a history of allergic reactions to insect stings were assessed. The mean age at the time of the reactions was 33.4 ± 15.1 years (range, 4-76 years), and patients were evaluated 43.6 ± 90 months (range, 1-300 months) after the reactions. Clinical reactions were scored according to severity, from 1 (cutaneous manifestations only) to 3 (anaphylaxis with shock). These scores were compared to scores for skin Test reactivity (0 to 5, indicating the log increase in sensitivity from 1 μg/mL to 0.0001 μg/mL) and Radioallergosorbent Test (RAST) levels (0 to 4, indicating venom-specific IgE levels, from undetectable to >17.5 kilounits of antigen per litre [kUA/L]). Results No correlation was found between skin Test reactivity (Spearman's coefficient = 0.15, p = .377) or RAST level (Spearman's coefficient = 0.32, p = .061) and the severity of reaction. Skin Test and RAST scores both differed significantly from clinical severity ( p < .05), but there was a significant correlation between skin Test reactivity and RAST score ( p = .042). There was no correlation between skin Test reactivity and time since reaction (Spearman's coefficient = 0.18, p = .294) nor between RAST and time since reaction ( r = 0.1353, p = .438). Elimination of patients Tested more than 12 months after their reaction still produced no correlation between skin Test reactivity ( p = .681) or RAST score ( p = .183) and the severity of the clinical reaction. Conclusion In venom-allergic patients (in contrast to reported findings in cases of inhalant IgE-mediated allergy), there appears to be no significant correlation between the degree of skin Test reactivity or levels of venom-specific IgE (determined by RAST) and the severity of the clinical reaction.
Zhang Hong-yu - One of the best experts on this subject based on the ideXlab platform.
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Evaluate the Total Potency of Allergen Extract by Radioallergosorbent Test Inhibition
Chinese Journal of Allergy and Clinical Immunology, 2007Co-Authors: Zhang Hong-yuAbstract:To evaluate the total potency of allergen extract by Radioallergosorbent Test(RAST) inhibition.Methods The in-house reference(IHR)of mugwort pollen extract was prepared.Mugwort pollen extract A was prepared follow the same operation procedure,10-fold diluted extract A was definited as extract B.RAST inhibition was done.The inhibition curves was protracted and the 50% inhibition dilution was calculated.Results There was no significant difference between the slope of IHR and the slope of extract A and B.50% inhibition dilution of IHR was 10~(-3.61),50% inhibition dilution of extract A and B was 10~(-3.57)and 10~(-2.62)separately.Compared with IHR,the relative potency of extract A and B was 1 and 0.1 approximately.Conclusions The total potency of allergen extract can be evaluated by RAST inhibition.Quality control of allergen extract can be done based on the relative potency compare to the standardization or the in-house reference.
S. Torii - One of the best experts on this subject based on the ideXlab platform.
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cross allergenicity between aspergillus restrictus aspergillus fumigatus and alternaria alternata determined by Radioallergosorbent Test inhibition
Allergology International, 1996Co-Authors: Tatsuo Sakamoto, Masanori Yamada, Mio Miyake, S. ToriiAbstract:Aspergillus restrictus, an osmophilic fungus, is abundant in house dust. We have shown previously that the incidence of immediate hypersensitivity to A. restrictus is comparable to that for Aspergillus fumigatus and Alternaria alternata in asthmatic children. Radioallergosorbent Test (RAST) inhibition was used to determine whether A. restrictus shares similar allergenic components with A. fumigatus and A. alternata. Mycelial mats of the three species cultivated on completely synthetic media were used for extract preparation. IgE antibodies to each fungus were measured with RAST using a polyvinyl chloride microplate as a solid phase. Analysis of a serum pool obtained from nine asthmatic children with a positive RAST to A. restrictus showed that A. restrictus inhibited the RAST to A. restrictus, A. fumigatus and A. alternata by more than 80%. Similar results were observed with A. fumigatus and A. alternata. Additionally, when 13 serum samples with a positive RAST to A. restrictus were Tested separately, A. restrictus substantially inhibited the A. restrictus RAST in all subjects Tested. A. fumigatus and A. alternata inhibited the A. restrictus RAST in 10 and 8 of the samples studied, respectively. These findings indicate that A. restrictus shares allergenic components with A. fumigatus and A. alternata. The allergenic cross-reactivity between A. fumigatus and A. alternata was also demonstrated.
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allergenic relationships between aspergillus restrictus and aspergillus fumigatus determined by Radioallergosorbent Test inhibition
Arerugī (Allergy), 1991Co-Authors: Tatsuo Sakamoto, Masanori Yamada, S. Torii, Yoshio MatsudaAbstract:: Recently Aspergillus restrictus, a species of osmophilic fungi, has been found to be widely distributed in our inhabited environment. The authors reported previously that the prevalence of hypersensitivity to this fungal species determined by skin prick Test and Radioallergosorbent Test (RAST) was comparable with that to Aspergillus fumigatus. In this study, the allergenic relationships between the two fungi were examined by RAST and RAST inhibition Tests in which A. restrictus extracts prepared from mycelial mats of two different strains cultured in M40Y liquid medium and A. fumigatus, one made from a mycelial mat cultured in YNB liquid medium containing 3% sucrose, were used. In the RAST inhibition Tests using a serum pool obtained from five patients with highly positive RAST to these species, the two extracts of A. restrictus inhibited RAST to A. fumigatus by over 80%, and A. fumigatus extract inhibited RAST to the two strains of A. restrictus by approximately 75%. In A. fumigatus RAST inhibition Tests using individual sera with positive A. fumigatus RAST, a strong inhibition (greater than 60%) elicited by each extract of A. restrictus was observed in 5 of 8 sera Tested. In RAST, nine of 11 serum samples with positive RAST to A. fumigatus showed positive RAST to both strains of A. restrictus. There was a significant correlation between the RAST values of the two fungal species. These results give evidence of the presence of shared allergenic components between the two fungi, and indicate that these components may contain part of major allergens of A. fumigatus.
William K Dolen - One of the best experts on this subject based on the ideXlab platform.
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cross reactivity between cockroach and ladybug using the Radioallergosorbent Test
Annals of Allergy Asthma & Immunology, 2009Co-Authors: Matthew T Clark, Todd Levin, William K DolenAbstract:Background Home infestations from Harmonia axyridis (ladybug) occur throughout the United States. IgE-mediated sensitization with allergic disease has been reported. The prevalence of ladybug sensitization has been studied by questionnaire and allergy Testing in patients diagnosed as having allergic disease. Cross-reactivity with cockroach exists. Objectives To determine the prevalence of ladybug specific IgE in the general population by specific IgE immunoassay and to examine cross-reactivity to cockroach. Methods An experimental solid phase for use in immunoassay was prepared using a ladybug extract, and performance characteristics were determined. Serum samples from 100 adult blood bank donors were Tested using the ladybug specific IgE immunoassay. Known ladybug IgE-positive serum samples obtained from symptomatic patients were used to study cross-reactivity with German cockroach by specific IgE immunoassay inhibition. Results The mean background response of the assay solid phase was 51 fluorescent units with an analytical cutoff of 59 fluorescent units. It was estimated that a response of 88 fluorescent units corresponds to a specific IgE concentration of 0.1 kUa/L. The extinction dilution curve was linear to 0.1 kUa/L. The assay cutoff was set at 0.1 kUa/L. Of the 100 blood donor serum samples, 10 were positive for ladybug specific IgE. Inhibition assays revealed partial cross-reactivity with German cockroach. Conclusion Although an immunoassay solid phase prepared with ladybug whole body extract will identify persons in a general population sensitized to epitopes found in the extract, clinicians performing allergy Testing using whole body ladybug extract should be aware that a positive result may or may not indicate that exposure to actual ladybug allergens is causing sensitization.
Erliyani Sartono - One of the best experts on this subject based on the ideXlab platform.
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comparison of an enzyme linked immunosorbent assay elisa and a Radioallergosorbent Test rast for detection of ige antibodies to brugia malayi
Parasite Immunology, 2003Co-Authors: Sitti Wahyuni, Ronald Van Ree, Andarias Mangali, Taniawati Supali, Maria Yazdanbakhsh, Erliyani SartonoAbstract:SUMMARY The enzyme linked immunosorbent assay (ELISA) for specific IgE antibodies to Brugia malayi was compared with the Radioallergosorbent Test (RAST) for use in immunoepidemiological studies of lymphatic filariasis. Sera used were from individuals (aged 5–82 years) living in an area endemic for lymphatic filariasis in South Sulawesi, Indonesia. The percentage of positive IgE ELISA reactions (52·6%) among the population was lower than the percentage of positive RAST (94·5%). Although an overall significant concordance was found between the two assays (P < 0·001), 328 (42·7%) individuals with a positive RAST result were negative in the ELISA, whereas only 6 (0·8%) subjects were positive by ELISA, yet negative by RAST. When the population was divided into those with active infection (positive for anti-filarial IgG4) and those not infected (mf-negative and negative for anti-filarial IgG4), the correlation between the two Tests was higher in the IgG4-positive (rho = 0·70) than in the IgG4-negative (rho = 0·52) group. These results indicate that in assessment of B. malayi specific IgE antibody, RAST is superior to ELISA. However, given the use of radioactivity in the RAST method and given our results obtained in subjects with high anti-filarial IgG4, one could consider using the IgE-ELISA in areas with high endemicity for filariasis. In areas with low endemicity or where control programs are implemented, sera will have to be Tested by RAST.
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Comparison of an enzyme linked immunosorbent assay (ELISA) and a Radioallergosorbent Test (RAST) for detection of IgE antibodies to Brugia malayi.
Parasite immunology, 2003Co-Authors: Sitti Wahyuni, Ronald Van Ree, Andarias Mangali, Taniawati Supali, Maria Yazdanbakhsh, Erliyani SartonoAbstract:SUMMARY The enzyme linked immunosorbent assay (ELISA) for specific IgE antibodies to Brugia malayi was compared with the Radioallergosorbent Test (RAST) for use in immunoepidemiological studies of lymphatic filariasis. Sera used were from individuals (aged 5–82 years) living in an area endemic for lymphatic filariasis in South Sulawesi, Indonesia. The percentage of positive IgE ELISA reactions (52·6%) among the population was lower than the percentage of positive RAST (94·5%). Although an overall significant concordance was found between the two assays (P
