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James P Mcnamee - One of the best experts on this subject based on the ideXlab platform.
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analysis of gene expression in two human derived cell lines exposed in vitro to a 1 9 ghz pulse modulated Radiofrequency Field
Proteomics, 2007Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, Carole L Yauk, George R Douglas, Andrew Williams, A Mariampillai, Stephanie Lui, James P McnameeAbstract:There is considerable controversy surrounding the biological effects of Radiofrequency (RF) Fields, as emitted by mobile phones. Previous work from our laboratory has shown no effect related to the exposure of 1.9 GHz pulse-modulated RF Fields on the expression of 22,000 genes in a human glioblastoma-derived cell-line (U87MG) at 6 h following a 4 h RF Field exposure period. As a follow-up to this study, we have now examined the effect of RF Field exposure on the possible expression of late onset genes in U87MG cells after a 24 h RF exposure period. In addition, a human monocyte-derived cell-line (Mono-Mac-6, MM6) was exposed to intermittent (5 min ON, 10 min OFF) RF Fields for 6 h and then gene expression was assessed immediately after exposure and at 18 h postexposure. Both cell lines were exposed to 1.9 GHz pulse-modulated RF Fields for 6 or 24 h at specific absorption rates (SARs) of 0.1-10.0 W/kg. In support of our previous results, we found no evidence that nonthermal RF Field exposure could alter gene expression in either cultured U87MG or MM6 cells, relative to nonirradiated control groups. However, exposure of both cell-lines to heat-shock conditions (43 degrees C for 1 h) caused an alteration in the expression of a number of well-characterized heat-shock proteins.
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analysis of proto oncogene and heat shock protein gene expression in human derived cell lines exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
International Journal of Radiation Biology, 2006Co-Authors: Vinita Chauhan, G B Gajda, A Thansandote, A Mariampillai, James P McnameeAbstract:Purpose: Several studies have reported that Radiofrequency (RF) Fields, as emitted by mobile phones, may cause changes in gene expression in cultured human cell-lines. The current study was undertaken to evaluate this possibility in two human-derived immune cell-lines.Materials and methods: HL-60 and Mono-Mac-6 (MM6) cells were individually exposed to intermittent (5 min on, 10 min off) 1.9 GHz pulse-modulated RF Fields at a average specific absorption rate (SAR) of 1 and 10 W/kg at 37 ± 0.5°C for 6 h. Concurrent negative and positive (heat-shock for 1 h at 43°C) controls were conducted with each experiment. Immediately following RF Field exposure (T = 6 h) and 18 h post-exposure (T = 24 h), cell pellets were collected from each of the culture dishes and analyzed for transcript levels of proto-oncogenes (c-jun, c-myc and c-fos) and the stress-related genes (heat shock proteins (HSP) HSP27 and HSP70B) by quantitative reverse transcriptase polymerase chain reaction (RT-PCR).Results: No significant effects w...
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gene expression analysis of a human lymphoblastoma cell line exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, G B Gajda, E Lemay, A Thansandote, A Mariampillai, James P McnameeAbstract:Abstract Chauhan, V., Mariampillai, A., Bellier, P. V., Qutob, S. S., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 424–429 (2006). This study was designed to determine whether Radiofrequency (RF) Fields of the type used for wireless communications could elicit a cellular stress response. As general indicators of a cellular stress response, we monitored changes in proto-oncogene and heat-shock protein expression. Exponentially growing human lymphoblastoma cells (TK6) were exposed to 1.9 GHz pulse-modulated RF Fields at average specific absorption rates (SARs) of 1 and 10 W/kg. Perturbations in the expression levels of the proto-oncogenes FOS, JUN and MYC after exposure to sham and RF Fields were assessed by real-time RT-PCR. In addition, the transcript levels of the cellular stress proteins HSP27 and inducible HSP70 were also monitor...
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dna damage in human leukocytes after acute in vitro exposure to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Lavallee, B. F., Lemay, E. P., Marro, L. and Thansandote, A. DNA Damage in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 158, 534–537 (2002). Blood cultures from human volunteers were exposed to an acute 1.9 GHz pulse-modulated Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) ranged from 0 to 10 W/kg, and the temperature within the cultures during the exposure was maintained at 37.0 ± 0.5°C. DNA damage was quantified in leukocytes by the alkaline comet assay and the cytokinesis-block micronucleus assay. When compared to the sham-treated controls, no evidence of increased primary DNA damage was detected by any parameter for any of the RF-Field-exposed cultures when evaluated using the alkaline comet assay. Furthermore, no significant differences in the frequency of binucleated cells, incidence of mi...
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dna damage and micronucleus induction in human leukocytes after acute in vitro exposure to a 1 9 ghz continuous wave Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, Susan M Miller, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay E. P., Lavallee, B. F., Marro, L. and Thansandote, A. DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field. Radiat. Res. 158, 523–533 (2002). Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h exposure was maintained at 37.0 ± 0.5°C. Concurrent negative (incubator) and positive (1.5 Gy 137Cs γ radiation) control cultures were run for each experiment. DNA damage was quantified immediately after RF-Field exposure using the alkaline comet assay, and four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. No evidence of increase...
A Thansandote - One of the best experts on this subject based on the ideXlab platform.
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microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Sami S Qutob, Vinita Chauhan, P V Bellier, Carole L Yauk, George R Douglas, Lynn Berndt, Andrew Williams, G B Gajda, E Lemay, A ThansandoteAbstract:Abstract Qutob, S. S., Chauhan, V., Bellier, P. V., Yauk, C. L., Douglas, G. R., Berndt, L., Williams, A., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Microarray Gene Expression Profiling of a Human Glioblastoma Cell Line Exposed In Vitro to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 636–644 (2006). The widespread use of mobile phones has led to public concerns about the health effects associated with exposure to Radiofrequency (RF) Fields. The paramount concern of most persons relates to the potential of these Fields to cause cancer. Unlike ionizing radiation, RF Fields used for mobile telecommunications (800–1900 MHz) do not possess sufficient energy to directly damage DNA. Most rodent bioassay and in vitro genotoxicity/mutation studies have reported that RF Fields at non-thermal levels have no direct mutagenic, genotoxic or carcinogenic effects. However, some evidence has suggested that RF Fields may cause detectable postexposure changes in gene expression. Theref...
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analysis of proto oncogene and heat shock protein gene expression in human derived cell lines exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
International Journal of Radiation Biology, 2006Co-Authors: Vinita Chauhan, G B Gajda, A Thansandote, A Mariampillai, James P McnameeAbstract:Purpose: Several studies have reported that Radiofrequency (RF) Fields, as emitted by mobile phones, may cause changes in gene expression in cultured human cell-lines. The current study was undertaken to evaluate this possibility in two human-derived immune cell-lines.Materials and methods: HL-60 and Mono-Mac-6 (MM6) cells were individually exposed to intermittent (5 min on, 10 min off) 1.9 GHz pulse-modulated RF Fields at a average specific absorption rate (SAR) of 1 and 10 W/kg at 37 ± 0.5°C for 6 h. Concurrent negative and positive (heat-shock for 1 h at 43°C) controls were conducted with each experiment. Immediately following RF Field exposure (T = 6 h) and 18 h post-exposure (T = 24 h), cell pellets were collected from each of the culture dishes and analyzed for transcript levels of proto-oncogenes (c-jun, c-myc and c-fos) and the stress-related genes (heat shock proteins (HSP) HSP27 and HSP70B) by quantitative reverse transcriptase polymerase chain reaction (RT-PCR).Results: No significant effects w...
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gene expression analysis of a human lymphoblastoma cell line exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, G B Gajda, E Lemay, A Thansandote, A Mariampillai, James P McnameeAbstract:Abstract Chauhan, V., Mariampillai, A., Bellier, P. V., Qutob, S. S., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 424–429 (2006). This study was designed to determine whether Radiofrequency (RF) Fields of the type used for wireless communications could elicit a cellular stress response. As general indicators of a cellular stress response, we monitored changes in proto-oncogene and heat-shock protein expression. Exponentially growing human lymphoblastoma cells (TK6) were exposed to 1.9 GHz pulse-modulated RF Fields at average specific absorption rates (SARs) of 1 and 10 W/kg. Perturbations in the expression levels of the proto-oncogenes FOS, JUN and MYC after exposure to sham and RF Fields were assessed by real-time RT-PCR. In addition, the transcript levels of the cellular stress proteins HSP27 and inducible HSP70 were also monitor...
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dna damage in human leukocytes after acute in vitro exposure to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Lavallee, B. F., Lemay, E. P., Marro, L. and Thansandote, A. DNA Damage in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 158, 534–537 (2002). Blood cultures from human volunteers were exposed to an acute 1.9 GHz pulse-modulated Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) ranged from 0 to 10 W/kg, and the temperature within the cultures during the exposure was maintained at 37.0 ± 0.5°C. DNA damage was quantified in leukocytes by the alkaline comet assay and the cytokinesis-block micronucleus assay. When compared to the sham-treated controls, no evidence of increased primary DNA damage was detected by any parameter for any of the RF-Field-exposed cultures when evaluated using the alkaline comet assay. Furthermore, no significant differences in the frequency of binucleated cells, incidence of mi...
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dna damage and micronucleus induction in human leukocytes after acute in vitro exposure to a 1 9 ghz continuous wave Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, Susan M Miller, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay E. P., Lavallee, B. F., Marro, L. and Thansandote, A. DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field. Radiat. Res. 158, 523–533 (2002). Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h exposure was maintained at 37.0 ± 0.5°C. Concurrent negative (incubator) and positive (1.5 Gy 137Cs γ radiation) control cultures were run for each experiment. DNA damage was quantified immediately after RF-Field exposure using the alkaline comet assay, and four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. No evidence of increase...
P V Bellier - One of the best experts on this subject based on the ideXlab platform.
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analysis of gene expression in two human derived cell lines exposed in vitro to a 1 9 ghz pulse modulated Radiofrequency Field
Proteomics, 2007Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, Carole L Yauk, George R Douglas, Andrew Williams, A Mariampillai, Stephanie Lui, James P McnameeAbstract:There is considerable controversy surrounding the biological effects of Radiofrequency (RF) Fields, as emitted by mobile phones. Previous work from our laboratory has shown no effect related to the exposure of 1.9 GHz pulse-modulated RF Fields on the expression of 22,000 genes in a human glioblastoma-derived cell-line (U87MG) at 6 h following a 4 h RF Field exposure period. As a follow-up to this study, we have now examined the effect of RF Field exposure on the possible expression of late onset genes in U87MG cells after a 24 h RF exposure period. In addition, a human monocyte-derived cell-line (Mono-Mac-6, MM6) was exposed to intermittent (5 min ON, 10 min OFF) RF Fields for 6 h and then gene expression was assessed immediately after exposure and at 18 h postexposure. Both cell lines were exposed to 1.9 GHz pulse-modulated RF Fields for 6 or 24 h at specific absorption rates (SARs) of 0.1-10.0 W/kg. In support of our previous results, we found no evidence that nonthermal RF Field exposure could alter gene expression in either cultured U87MG or MM6 cells, relative to nonirradiated control groups. However, exposure of both cell-lines to heat-shock conditions (43 degrees C for 1 h) caused an alteration in the expression of a number of well-characterized heat-shock proteins.
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microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Sami S Qutob, Vinita Chauhan, P V Bellier, Carole L Yauk, George R Douglas, Lynn Berndt, Andrew Williams, G B Gajda, E Lemay, A ThansandoteAbstract:Abstract Qutob, S. S., Chauhan, V., Bellier, P. V., Yauk, C. L., Douglas, G. R., Berndt, L., Williams, A., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Microarray Gene Expression Profiling of a Human Glioblastoma Cell Line Exposed In Vitro to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 636–644 (2006). The widespread use of mobile phones has led to public concerns about the health effects associated with exposure to Radiofrequency (RF) Fields. The paramount concern of most persons relates to the potential of these Fields to cause cancer. Unlike ionizing radiation, RF Fields used for mobile telecommunications (800–1900 MHz) do not possess sufficient energy to directly damage DNA. Most rodent bioassay and in vitro genotoxicity/mutation studies have reported that RF Fields at non-thermal levels have no direct mutagenic, genotoxic or carcinogenic effects. However, some evidence has suggested that RF Fields may cause detectable postexposure changes in gene expression. Theref...
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gene expression analysis of a human lymphoblastoma cell line exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, G B Gajda, E Lemay, A Thansandote, A Mariampillai, James P McnameeAbstract:Abstract Chauhan, V., Mariampillai, A., Bellier, P. V., Qutob, S. S., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 424–429 (2006). This study was designed to determine whether Radiofrequency (RF) Fields of the type used for wireless communications could elicit a cellular stress response. As general indicators of a cellular stress response, we monitored changes in proto-oncogene and heat-shock protein expression. Exponentially growing human lymphoblastoma cells (TK6) were exposed to 1.9 GHz pulse-modulated RF Fields at average specific absorption rates (SARs) of 1 and 10 W/kg. Perturbations in the expression levels of the proto-oncogenes FOS, JUN and MYC after exposure to sham and RF Fields were assessed by real-time RT-PCR. In addition, the transcript levels of the cellular stress proteins HSP27 and inducible HSP70 were also monitor...
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dna damage in human leukocytes after acute in vitro exposure to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Lavallee, B. F., Lemay, E. P., Marro, L. and Thansandote, A. DNA Damage in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 158, 534–537 (2002). Blood cultures from human volunteers were exposed to an acute 1.9 GHz pulse-modulated Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) ranged from 0 to 10 W/kg, and the temperature within the cultures during the exposure was maintained at 37.0 ± 0.5°C. DNA damage was quantified in leukocytes by the alkaline comet assay and the cytokinesis-block micronucleus assay. When compared to the sham-treated controls, no evidence of increased primary DNA damage was detected by any parameter for any of the RF-Field-exposed cultures when evaluated using the alkaline comet assay. Furthermore, no significant differences in the frequency of binucleated cells, incidence of mi...
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dna damage and micronucleus induction in human leukocytes after acute in vitro exposure to a 1 9 ghz continuous wave Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, Susan M Miller, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay E. P., Lavallee, B. F., Marro, L. and Thansandote, A. DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field. Radiat. Res. 158, 523–533 (2002). Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h exposure was maintained at 37.0 ± 0.5°C. Concurrent negative (incubator) and positive (1.5 Gy 137Cs γ radiation) control cultures were run for each experiment. DNA damage was quantified immediately after RF-Field exposure using the alkaline comet assay, and four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. No evidence of increase...
G B Gajda - One of the best experts on this subject based on the ideXlab platform.
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microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Sami S Qutob, Vinita Chauhan, P V Bellier, Carole L Yauk, George R Douglas, Lynn Berndt, Andrew Williams, G B Gajda, E Lemay, A ThansandoteAbstract:Abstract Qutob, S. S., Chauhan, V., Bellier, P. V., Yauk, C. L., Douglas, G. R., Berndt, L., Williams, A., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Microarray Gene Expression Profiling of a Human Glioblastoma Cell Line Exposed In Vitro to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 636–644 (2006). The widespread use of mobile phones has led to public concerns about the health effects associated with exposure to Radiofrequency (RF) Fields. The paramount concern of most persons relates to the potential of these Fields to cause cancer. Unlike ionizing radiation, RF Fields used for mobile telecommunications (800–1900 MHz) do not possess sufficient energy to directly damage DNA. Most rodent bioassay and in vitro genotoxicity/mutation studies have reported that RF Fields at non-thermal levels have no direct mutagenic, genotoxic or carcinogenic effects. However, some evidence has suggested that RF Fields may cause detectable postexposure changes in gene expression. Theref...
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analysis of proto oncogene and heat shock protein gene expression in human derived cell lines exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
International Journal of Radiation Biology, 2006Co-Authors: Vinita Chauhan, G B Gajda, A Thansandote, A Mariampillai, James P McnameeAbstract:Purpose: Several studies have reported that Radiofrequency (RF) Fields, as emitted by mobile phones, may cause changes in gene expression in cultured human cell-lines. The current study was undertaken to evaluate this possibility in two human-derived immune cell-lines.Materials and methods: HL-60 and Mono-Mac-6 (MM6) cells were individually exposed to intermittent (5 min on, 10 min off) 1.9 GHz pulse-modulated RF Fields at a average specific absorption rate (SAR) of 1 and 10 W/kg at 37 ± 0.5°C for 6 h. Concurrent negative and positive (heat-shock for 1 h at 43°C) controls were conducted with each experiment. Immediately following RF Field exposure (T = 6 h) and 18 h post-exposure (T = 24 h), cell pellets were collected from each of the culture dishes and analyzed for transcript levels of proto-oncogenes (c-jun, c-myc and c-fos) and the stress-related genes (heat shock proteins (HSP) HSP27 and HSP70B) by quantitative reverse transcriptase polymerase chain reaction (RT-PCR).Results: No significant effects w...
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gene expression analysis of a human lymphoblastoma cell line exposed in vitro to an intermittent 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2006Co-Authors: Vinita Chauhan, Sami S Qutob, P V Bellier, G B Gajda, E Lemay, A Thansandote, A Mariampillai, James P McnameeAbstract:Abstract Chauhan, V., Mariampillai, A., Bellier, P. V., Qutob, S. S., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. P. Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 424–429 (2006). This study was designed to determine whether Radiofrequency (RF) Fields of the type used for wireless communications could elicit a cellular stress response. As general indicators of a cellular stress response, we monitored changes in proto-oncogene and heat-shock protein expression. Exponentially growing human lymphoblastoma cells (TK6) were exposed to 1.9 GHz pulse-modulated RF Fields at average specific absorption rates (SARs) of 1 and 10 W/kg. Perturbations in the expression levels of the proto-oncogenes FOS, JUN and MYC after exposure to sham and RF Fields were assessed by real-time RT-PCR. In addition, the transcript levels of the cellular stress proteins HSP27 and inducible HSP70 were also monitor...
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dna damage in human leukocytes after acute in vitro exposure to a 1 9 ghz pulse modulated Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Lavallee, B. F., Lemay, E. P., Marro, L. and Thansandote, A. DNA Damage in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 158, 534–537 (2002). Blood cultures from human volunteers were exposed to an acute 1.9 GHz pulse-modulated Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) ranged from 0 to 10 W/kg, and the temperature within the cultures during the exposure was maintained at 37.0 ± 0.5°C. DNA damage was quantified in leukocytes by the alkaline comet assay and the cytokinesis-block micronucleus assay. When compared to the sham-treated controls, no evidence of increased primary DNA damage was detected by any parameter for any of the RF-Field-exposed cultures when evaluated using the alkaline comet assay. Furthermore, no significant differences in the frequency of binucleated cells, incidence of mi...
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dna damage and micronucleus induction in human leukocytes after acute in vitro exposure to a 1 9 ghz continuous wave Radiofrequency Field
Radiation Research, 2002Co-Authors: James P Mcnamee, P V Bellier, G B Gajda, E Lemay, B F Lavallee, L Marro, Susan M Miller, A ThansandoteAbstract:Abstract McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay E. P., Lavallee, B. F., Marro, L. and Thansandote, A. DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field. Radiat. Res. 158, 523–533 (2002). Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) Radiofrequency (RF) Field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h exposure was maintained at 37.0 ± 0.5°C. Concurrent negative (incubator) and positive (1.5 Gy 137Cs γ radiation) control cultures were run for each experiment. DNA damage was quantified immediately after RF-Field exposure using the alkaline comet assay, and four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. No evidence of increase...
Emmanuelle Poque - One of the best experts on this subject based on the ideXlab platform.
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Effects of Radiofrequency Field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique
Cell Stress and Chaperones, 2020Co-Authors: Emmanuelle Poque, Hermanus J. Ruigrok, Delia Arnaud-cormos, Denis Habauzit, Yann Chappe, Catherine Martin, Florence Poulletier De Gannes, Annabelle Hurtier, Andre Garenne, Isabelle LagroyeAbstract:As of today, only acute effects of RF Fields have been confirmed to represent a potential health hazard and they are attributed to non-specific heating (≥ 1 °C) under high-level exposure. Yet, the possibility that environmental RF impact living matter in the absence of temperature elevation needs further investigation. Since HSF1 is both a thermosensor and the master regulator of heat-shock stress response in eukaryotes, it remains to assess HSF1 activation in live cells under exposure to low-level RF signals. We thus measured basal, temperature-induced, and chemically induced HSF1 trimerization, a mandatory step on the cascade of HSF1 activation, under RF exposure to continuous wave (CW), Global System for Mobile (GSM), and Wi-Fi-modulated 1800 MHz signals, using a bioluminescence resonance energy transfer technique (BRET) probe. Our results show that, as expected, HSF1 is heat-activated by acute exposure of transiently transfected HEK293T cells to a CW RF Field at a specific absorption rate of 24 W/kg for 30 min. However, we found no evidence of HSF1 activation under the same RF exposure condition when the cell culture medium temperature was fixed. We also found no experimental evidence that, at a fixed temperature, chronic RF exposure for 24 h at a SAR of 1.5 and 6 W/kg altered the potency or the maximal capability of the proteasome inhibitor MG132 to activate HSF1, whatever signal used. We only found that RF exposure to CW signals (1.5 and 6 W/kg) and GSM signals (1.5 W/kg) for 24 h marginally decreased basal HSF1 activity.
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effects of Radiofrequency Fields on ras and erk kinases activity in live cells using the bioluminescence resonance energy transfer technique
International Journal of Radiation Biology, 2020Co-Authors: Emmanuelle Poque, Florence Poulletier De Gannes, Annabelle Hurtier, Andre Garenne, Isabelle Lagroye, Hermanus Ruigrok, Delia Arnaudcormos, Lorenza Patrignoni, Remy Renom, Philippe LevequeAbstract:Purpose: The present study was conducted to re-evaluate the effect of low-level 1800 MHz RF signals on RAS/MAPK activation in live cells.Material and methods: Using Bioluminescence Resonance Energy Transfer technique (BRET), we assessed the effect of Continuous wave (CW) and Global System for Mobile (GSM)-modulated 1800 MHz signals (up to 2 W/kg) on ERK and RAS kinases' activity in live HuH7 cells.Results: We found that Radiofrequency Field (RF) exposure for 24 h altered neither basal level of RAS and ERK activation nor the potency of phorbol-12-myristate-13-acetate (PMA) to activate RAS and ERK kinases. However, we found that exposure to GSM-modulated 1800 MHz signals at 2 W/kg decreased the PMA maximal efficacy to activate both RAS and ERK kinases' activity. Exposure with CW 1800 MHz signal at 2 W/kg only decreased maximal efficacy of PMA to activate ERK but not RAS. No effects of RF exposure at 0.5 W/kg was observed on maximal efficacy of PMA to activate either RAS or ERK whatever the signal used.Conclusions: Our results indicate that RF exposure decreases the efficiency of the cascade of events, which, from the binding of PMA to its receptor(s), leads to the activation of RAS and ERK kinases.
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Effects of Radiofrequency Field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique
Cell Stress and Chaperones, 2020Co-Authors: Emmanuelle Poque, Hermanus J. Ruigrok, Delia Arnaud-cormos, Denis Habauzit, Yann Chappe, Catherine Martin, Florence Poulletier De Gannes, Annabelle Hurtier, Andre Garenne, Isabelle LagroyeAbstract:As of today, only acute effects of RF Fields have been confirmed to represent a potential health hazard and they are attributed to non-specific heating (>= 1 degrees C) under high-level exposure. Yet, the possibility that environmental RF impact living matter in the absence of temperature elevation needs further investigation. Since HSF1 is both a thermosensor and the master regulator of heat-shock stress response in eukaryotes, it remains to assess HSF1 activation in live cells under exposure to low-level RF signals. We thus measured basal, temperature-induced, and chemically induced HSF1 trimerization, a mandatory step on the cascade of HSF1 activation, under RF exposure to continuous wave (CW), Global System for Mobile (GSM), and Wi-Fi-modulated 1800 MHz signals, using a bioluminescence resonance energy transfer technique (BRET) probe. Our results show that, as expected, HSF1 is heat-activated by acute exposure of transiently transfected HEK293T cells to a CW RF Field at a specific absorption rate of 24 W/kg for 30 min. However, we found no evidence of HSF1 activation under the same RF exposure condition when the cell culture medium temperature was fixed. We also found no experimental evidence that, at a fixed temperature, chronic RF exposure for 24 h at a SAR of 1.5 and 6 W/kg altered the potency or the maximal capability of the proteasome inhibitor MG132 to activate HSF1, whatever signal used. We only found that RF exposure to CW signals (1.5 and 6 W/kg) and GSM signals (1.5 W/kg) for 24 h marginally decreased basal HSF1 activity.
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activation of the trpv1 thermoreceptor induced by modulated or unmodulated 1800 mhz Radiofrequency Field exposure
Radiation Research, 2017Co-Authors: Emmanuelle Poque, Florence Poulletier De Gannes, Annabelle Hurtier, Hermanus Ruigrok, Delia Arnaudcormos, G RuffieAbstract:The existence of effects of Radiofrequency Field exposure at environmental levels on living tissues and organisms remains controversial, in particular regarding potential “nonthermal” effects produced in the absence of temperature elevation. Therefore, we investigated whether TRPV1, one of the most studied thermosensitive channels, can be activated by the heat produced by Radiofrequency Fields and by some specific nonthermal interaction with the Fields. We have recently shown that TRPV1 activation can be assessed in real-time on live cells using the bioluminescence resonance energy transfer technique. Taking advantage of this innovative assay, we monitored TRPV1 thermal and chemical modes of activation under Radiofrequency exposure at 1800 MHz using different signals (CW, GSM, UMTS, LTE, Wi-Fi and WiMAX) at specific absorption rates between 8 and 32 W/kg. We showed that, as expected, TRPV1 channels were activated by the heat produced by Radiofrequency Field exposure of transiently-transfected HEK293T cell...