The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform
John W Griffin - One of the best experts on this subject based on the ideXlab platform.
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phosphorylation dependent immunoreactivity of neurofilaments and the rate of slow axonal transport in the central and peripheral axons of the rat dorsal root ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)
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Phosphorylation‐Dependent Immunoreactivity of Neurofilaments and the Rate of Slow Axonal Transport in the Central and Peripheral Axons of the Rat Dorsal Root Ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)
Soopeang Khor - One of the best experts on this subject based on the ideXlab platform.
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Gel electrophoresis-autoradiographic image analysis of Radiolabeled Protein drug concentration in serum for pharmacokinetic studies.
Journal of Pharmacological and Toxicological Methods, 2020Co-Authors: Di Song, Shang Ma, Soopeang KhorAbstract:Introduction: The purpose of this study was to evaluate the feasibility of using gel electrophoresis combined with autoradiographic image analysis for quantitating Protein drug concentrations in biological fluid for pharmacokinetic studies. Methods: Protein drugs were iodinated using the Iodogen reagent and injected into Sprague–Dawley rats for pharmacokinetic evaluation. Serum samples were analyzed using trichloroacetic acid (TCA)-precipitable counts or sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Commercially available precasted Bis–Tris gradient gels were used for SDS-PAGE. Autoradiography of gel samples was performed using phosphoimager and quantitated using the ImageQuant software. Results: The maximum loading volume for Protein drugs with molecular weight close to that of albumin (∼70 kDa) was about 1 μl, whereas for Protein drugs with larger or smaller molecular weight (i.e., >80 or
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gel electrophoresis autoradiographic image analysis of Radiolabeled Protein drug concentration in serum for pharmacokinetic studies
Journal of Pharmacological and Toxicological Methods, 2002Co-Authors: Di Song, Soopeang KhorAbstract:Introduction: The purpose of this study was to evaluate the feasibility of using gel electrophoresis combined with autoradiographic image analysis for quantitating Protein drug concentrations in biological fluid for pharmacokinetic studies. Methods: Protein drugs were iodinated using the Iodogen reagent and injected into Sprague–Dawley rats for pharmacokinetic evaluation. Serum samples were analyzed using trichloroacetic acid (TCA)-precipitable counts or sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Commercially available precasted Bis–Tris gradient gels were used for SDS-PAGE. Autoradiography of gel samples was performed using phosphoimager and quantitated using the ImageQuant software. Results: The maximum loading volume for Protein drugs with molecular weight close to that of albumin (∼70 kDa) was about 1 μl, whereas for Protein drugs with larger or smaller molecular weight (i.e., >80 or <40 kDa), the maximum loading volume was up to 20 μl/lane. The optimal exposure time was about 18 h or overnight. Standard curves were constructed using serially diluted dosing solution, which was linear over a 10-fold concentration range with a correlation coefficient of .98. Comparing to the soluble human interleukin-13 receptor (shIL-13R) pharmacokinetic profiles from TCA-precipitable counts, the quantitative gel analysis revealed lower concentrations at later time points and a lower bioavailability from intraperitoneal injection. Discussion: This study provided the first systemic evaluation of gel electrophoresis technology for quantitative Protein drug determination in serum and its application in pharmacokinetic studies. The combination of gel electrophoresis with autoradiographic image analysis provided accurate and specific quantitation results. The overnight turnover time allowed routine application in preclinical pharmacokinetic studies.
D R Archer - One of the best experts on this subject based on the ideXlab platform.
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phosphorylation dependent immunoreactivity of neurofilaments and the rate of slow axonal transport in the central and peripheral axons of the rat dorsal root ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)
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Phosphorylation‐Dependent Immunoreactivity of Neurofilaments and the Rate of Slow Axonal Transport in the Central and Peripheral Axons of the Rat Dorsal Root Ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)
Di Song - One of the best experts on this subject based on the ideXlab platform.
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Gel electrophoresis-autoradiographic image analysis of Radiolabeled Protein drug concentration in serum for pharmacokinetic studies.
Journal of Pharmacological and Toxicological Methods, 2020Co-Authors: Di Song, Shang Ma, Soopeang KhorAbstract:Introduction: The purpose of this study was to evaluate the feasibility of using gel electrophoresis combined with autoradiographic image analysis for quantitating Protein drug concentrations in biological fluid for pharmacokinetic studies. Methods: Protein drugs were iodinated using the Iodogen reagent and injected into Sprague–Dawley rats for pharmacokinetic evaluation. Serum samples were analyzed using trichloroacetic acid (TCA)-precipitable counts or sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Commercially available precasted Bis–Tris gradient gels were used for SDS-PAGE. Autoradiography of gel samples was performed using phosphoimager and quantitated using the ImageQuant software. Results: The maximum loading volume for Protein drugs with molecular weight close to that of albumin (∼70 kDa) was about 1 μl, whereas for Protein drugs with larger or smaller molecular weight (i.e., >80 or
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gel electrophoresis autoradiographic image analysis of Radiolabeled Protein drug concentration in serum for pharmacokinetic studies
Journal of Pharmacological and Toxicological Methods, 2002Co-Authors: Di Song, Soopeang KhorAbstract:Introduction: The purpose of this study was to evaluate the feasibility of using gel electrophoresis combined with autoradiographic image analysis for quantitating Protein drug concentrations in biological fluid for pharmacokinetic studies. Methods: Protein drugs were iodinated using the Iodogen reagent and injected into Sprague–Dawley rats for pharmacokinetic evaluation. Serum samples were analyzed using trichloroacetic acid (TCA)-precipitable counts or sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Commercially available precasted Bis–Tris gradient gels were used for SDS-PAGE. Autoradiography of gel samples was performed using phosphoimager and quantitated using the ImageQuant software. Results: The maximum loading volume for Protein drugs with molecular weight close to that of albumin (∼70 kDa) was about 1 μl, whereas for Protein drugs with larger or smaller molecular weight (i.e., >80 or <40 kDa), the maximum loading volume was up to 20 μl/lane. The optimal exposure time was about 18 h or overnight. Standard curves were constructed using serially diluted dosing solution, which was linear over a 10-fold concentration range with a correlation coefficient of .98. Comparing to the soluble human interleukin-13 receptor (shIL-13R) pharmacokinetic profiles from TCA-precipitable counts, the quantitative gel analysis revealed lower concentrations at later time points and a lower bioavailability from intraperitoneal injection. Discussion: This study provided the first systemic evaluation of gel electrophoresis technology for quantitative Protein drug determination in serum and its application in pharmacokinetic studies. The combination of gel electrophoresis with autoradiographic image analysis provided accurate and specific quantitation results. The overnight turnover time allowed routine application in preclinical pharmacokinetic studies.
D F Watson - One of the best experts on this subject based on the ideXlab platform.
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phosphorylation dependent immunoreactivity of neurofilaments and the rate of slow axonal transport in the central and peripheral axons of the rat dorsal root ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)
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Phosphorylation‐Dependent Immunoreactivity of Neurofilaments and the Rate of Slow Axonal Transport in the Central and Peripheral Axons of the Rat Dorsal Root Ganglion
Journal of Neurochemistry, 2008Co-Authors: D R Archer, D F Watson, John W GriffinAbstract:The rate of axonal transport of tubulin, actin, and the neurofilament Proteins was measured in the peripheral and central projections of the rat LS dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the «front» of the Radiolabeled Protein was located 7, 14, and 20 days postinlection. Transport rates calculated for the neurofilament triplet Proteins, tubulin, and actin in the peripheral nerve were ∼1.5-fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼1.7-fold; tubulin/actin, 2.1-fold)