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Philippe Prior - One of the best experts on this subject based on the ideXlab platform.
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Sequencing of K60, Type Strain of the Major Plant Pathogen Ralstonia solanacearum
Journal of Bacteriology, 2012Co-Authors: Benoît Remenant, Philippe Prior, Lavanya Babujee, Aurélie Lajus, Claudine Médigue, Caitilyn AllenAbstract:Ralstonia solanacearum is a widespread and destructive plant pathogen. We present the genome of the type strain, K60 (phylotype IIA, sequevar 7). Sequevar 7 strains cause ongoing tomato bacterial wilt outbreaks in the southeastern United States. K60 generally resembles R. solanacearum CFBP2957, a Caribbean tomato isolate, but has almost 360 unique genes.
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Evolutionary history of Ralstonia solanacearum inferred from MLSA
2012Co-Authors: Emmanuel Wicker, Pierre Lefeuvre, Jean-charles De Cambiaire, Christophe Lemaire, Stéphane Poussier, Philippe PriorAbstract:We used multilocus sequence analysis (MLSA) on a worldwide collection of the plant pathogenic Ralstonia solanacearum (Betaproteobacteria) to retrace its complex evolutionary history. Using genetic imprints left during Ralstonia solanacearum evolution, we were able to delineate distinct evolutionary complex displaying contrasting dynamics. Among the phylotypes already described (I, IIA, IIB, III, IV), eight groups of strains with distinct evolutionary patterns, named clades, were identified. From our recombination analysis, we identified 21 recombination events that occurred within and across these lineages. Although appearing the most divergent and ancestral phylotype, phylotype IV was inferred as a gene donor for the majority of the recombination events that we detected. Whereas this phylotype apparently fuelled the species diversity, ongoing diversification was mainly detected within phylotype I, IIA and III. These three groups presented a recent expanding population structure, a high level of homologous recombination, and evidences of long distance migrations. Factors such as adaptation to a specific host or intense trading of infected crops may have promoted this diversification. Whether Ralstonia solanacearum lineages will eventually evolve in distinct species remains difficult to predict. The intensification of cropping and increase of geographical dispersion may favour situations of phylotype sympatry and promote higher exchange of key factors for host adaptation from their common genetic pool. (Resume d'auteur)
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Complete Genome Sequence of the Plant Pathogen Ralstonia solanacearum Strain Po82
Journal of Bacteriology, 2011Co-Authors: Jin Xu, Philippe Prior, Huajun Zheng, Biao Tang, Jingsheng Xu, Hao Zhang, Qian Tian, Liqing ZhangAbstract:Ralstonia solanacearum strain Po82, a phylotype IIB/sequevar 4 strain, was found to be pathogenic to both solanaceous plants and banana. Here, we report the complete genome sequence of Po82 and its comparison with seven published R. solanacearum genomes.
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Broad Diversity of Ralstonia solanacearum Strains in Cameroon
Plant Disease, 2009Co-Authors: Gabriel Mahbou Somo Toukam, Caitilyn Allen, Emmanuel Wicker, Gilles Cellier, Caroline Guilbaud, Rémi Kahane, Philippe PriorAbstract:Mahbou Somo Toukam, G., Cellier, G., Wicker, E., Guilbaud, C., Kahane, R., Allen, C., and Prior, P. 2009. Broad diversity of Ralstonia solanacearum strains in Cameroon. Plant Dis. 93:1123-1130. In 2005, an extensive survey of bacterial wilt in Cameroon collected 110 strains of Ralstonia solanacearum from wilting tomato, potato, pepper, huckleberry (Solanum scabrum), sesame, and amaranth. The genetic diversity and phylogeny of selected strains from Cameroon were assessed by multiplex–polymerase chain reaction (PCR), race 3/biovar 2–specific PCR, and sequence analyses of the mutS and egl genes. These data were compared with those from 33 reference strains covering the known diversity within the R. solanacearum species complex. Strains isolated in Cameroon clustered into three of the four known phylotypes: I (Asian), II (American), and III (African). Lowland tomato strains belonged to phylotype I and were quite homogeneous. The strains belonging to phylotype II were genetically diverse, and partitioned into subclusters IIA and IIB (sequevar 1, race 3/biovar 2). Cameroon strains in the African phylotype III were distinct from reference strains from Zimbabwe or the Indian Ocean, highlighting the genetic diversity present within this phylotype. Strains from potatoes growing in the highlands of West Cameroon fell into both phylotypes II (race 3/biovar 2) and III. These phylotype II and III highland strains attacked both potato and tomato and could therefore pose an economic threat to potato and tomato crops throughout Central Africa. This is the first comprehensive report on the genetic diversity of R. solanacearum strains in Cameroon.
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Horizontal gene transfer between Ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays.
The ISME Journal, 2009Co-Authors: Alice Guidot, Philippe Prior, Bénédicte Coupat, Saliou Fall, Franck BertollaAbstract:Horizontal gene transfer between Ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays
Caitilyn Allen - One of the best experts on this subject based on the ideXlab platform.
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ralfuranone thioether production by the plant pathogen Ralstonia solanacearum
ChemBioChem, 2013Co-Authors: Julia Pauly, Caitilyn Allen, Dieter Spiteller, Jeanine Linz, Jonathan M Jacobs, Markus Nett, Dirk HoffmeisterAbstract:: Ralfuranones are aryl-substituted furanone secondary metabolites of the Gram-negative plant pathogen Ralstonia solanacearum. New sulfur-containing ralfuranone derivatives were identified, including the methyl thioether-containing ralfuranone D. Isotopic labeling in vivo, as well as headspace analyses of volatiles from R. solanacearum liquid cultures, established a mechanism for the transfer of an intact methylthio group from L-methionine or α-keto-γ-methylthiobutyric acid. The methylthio acceptor molecule ralfuranone I, a previously postulated biosynthetic intermediate in ralfuranone biosynthesis, was isolated and characterized by NMR. The highly reactive Michael acceptor system of this intermediate readily reacts with various thiols, including glutathione.
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Sequencing of K60, Type Strain of the Major Plant Pathogen Ralstonia solanacearum
Journal of Bacteriology, 2012Co-Authors: Benoît Remenant, Philippe Prior, Lavanya Babujee, Aurélie Lajus, Claudine Médigue, Caitilyn AllenAbstract:Ralstonia solanacearum is a widespread and destructive plant pathogen. We present the genome of the type strain, K60 (phylotype IIA, sequevar 7). Sequevar 7 strains cause ongoing tomato bacterial wilt outbreaks in the southeastern United States. K60 generally resembles R. solanacearum CFBP2957, a Caribbean tomato isolate, but has almost 360 unique genes.
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Broad Diversity of Ralstonia solanacearum Strains in Cameroon
Plant Disease, 2009Co-Authors: Gabriel Mahbou Somo Toukam, Caitilyn Allen, Emmanuel Wicker, Gilles Cellier, Caroline Guilbaud, Rémi Kahane, Philippe PriorAbstract:Mahbou Somo Toukam, G., Cellier, G., Wicker, E., Guilbaud, C., Kahane, R., Allen, C., and Prior, P. 2009. Broad diversity of Ralstonia solanacearum strains in Cameroon. Plant Dis. 93:1123-1130. In 2005, an extensive survey of bacterial wilt in Cameroon collected 110 strains of Ralstonia solanacearum from wilting tomato, potato, pepper, huckleberry (Solanum scabrum), sesame, and amaranth. The genetic diversity and phylogeny of selected strains from Cameroon were assessed by multiplex–polymerase chain reaction (PCR), race 3/biovar 2–specific PCR, and sequence analyses of the mutS and egl genes. These data were compared with those from 33 reference strains covering the known diversity within the R. solanacearum species complex. Strains isolated in Cameroon clustered into three of the four known phylotypes: I (Asian), II (American), and III (African). Lowland tomato strains belonged to phylotype I and were quite homogeneous. The strains belonging to phylotype II were genetically diverse, and partitioned into subclusters IIA and IIB (sequevar 1, race 3/biovar 2). Cameroon strains in the African phylotype III were distinct from reference strains from Zimbabwe or the Indian Ocean, highlighting the genetic diversity present within this phylotype. Strains from potatoes growing in the highlands of West Cameroon fell into both phylotypes II (race 3/biovar 2) and III. These phylotype II and III highland strains attacked both potato and tomato and could therefore pose an economic threat to potato and tomato crops throughout Central Africa. This is the first comprehensive report on the genetic diversity of R. solanacearum strains in Cameroon.
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bacterial wilt disease and the Ralstonia solanacearum species complex
Bacterial wilt disease and the Ralstonia solanacearum species complex., 2005Co-Authors: Caitilyn Allen, Philippe Prior, A C HaywardAbstract:Contents: Preface. Introductory overviews: Research on bacterial wilt: a perspective on international linkages and access to the literature; the current bacterial wilt situation: a global overview - Epidemiology of Ralstonia solanacearum: introduction and prospectus on the survival of introduction and prospectus on the survival; Fate of Ralstonia solanacearum Biovar 2 as affected by conditions and soil treatments in temperate climate zones; Mechanization has contributed to the spread of bacterial wilt on flue-cured tobacco in the Southeastern USA; Processes in the development of a biocontrol agent against bacterial wilt; Colonization capacity of Ralstonia solanacearum; Tomato strains differing in aggressiveness on tomatoes and weeds; Introduction to Europe of Ralstonia solanacearum Biovar 2, Race 3 in Pelargonium zonale cuttings from Kenya; Seeds from infected tomato plants appear to be free from contamination by Ralstonia solanacearum when tested by PCR or microbiological assays; The viable but non-culturable state in Ralstonia solanacearum: is there a realistic threat to our strategic concepts? - Bacterial wilt disease management: Management of bacterial wilt disease; Management of bacterial wilt in tomato with essential oils and systemic acquired resistance inducers; Monitoring of bacterial wilt in potato propagation material: a success story; Integrated control of potato bacterial wilt in Eastern Africa: the experience of African highlands initiative; Using Brassica spp. as biofumigants to reduce the population of Ralstonia solanacearum; Seed-plot technique: empowerment of farmers in production of bacterial wilt-free seed potato in Kenya and Uganda; Primary bacterial wilt study on tomato in vegetable areas of Ho Chi Minh City, Vietnam; Rhizome solarization and microwave treatment: ecofriendly methods for disinfecting ginger seed rhizomes; Management of bacterial wilt of potato using one-season rotation crops in South-Western Uganda; Potato bacterial wilt management: new prospects for an old problem - Breeding and deployment of wilt-resistant crops: A broad review and perspective on breeding for resistance to bacterial wilt; Progress on genetic enhancement for resistance to groundnut bacterial wilt in China; Search for resistance to bacterial wilt in a Brazilian Capsicum germplasm collection; Solanum phureja and S. stenotomum are sources of resistance to Ralstonia solanacearum for somatic hybrids of potato; Assessment of resistance to bacterial wilt in CIP advanced potato clones; Screening long pepper (Piper spp.) resistance to bacterial wilt caused by Ralstonia solanacearum - Host plant response and disease development: Host resistance to Ralstonia solanacearum; Microscopic studies of root infection in resistant tomato cv. Hawaii 7996; Development of bacterial wilt resistant varieties and basis of resistance in eggplant (Solanum melongena); QTL mapping for bacterial wilt resistance in Hawaii 7996 using AFLP, RGA, and SSR markers; Genetic basis of resistance to bacterial wilt in Arabidopsi thaliana; Roles of the Hrp-secreted PopA protein in Ralstonia solanacearum interactions with plants - Pathogen genetics: A short history of the biochemical and genetic research on Ralstonia solanacearum pathogenesis; The Ralstonia solanacearum complete genome sequence: outputs and prospects; Genes involved in early bacterial wilt pathogenesis; Phase reversion from phenotype conversion mutants to wild type may be induced in Ralstonia solanacearum by a susceptible host-plant; Insertions in the avirulence gene AvrA alter the virulence of Ralstonia solanacearum on Nicotiana tabacum; Ralstonia solanacearum requires type-4 Pili for twitching motility, adherence, natural transformation and virulence; Understanding the molecular basis of bacterial wilt disease: a view from the inside out - R. solanacearum in banana and plantains: Bacterial wilt diseases of banana: evolution and ecology; Comparative genome plasticity of tomato and banana strains o
Gaa Lle Huet - One of the best experts on this subject based on the ideXlab platform.
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breeding for resistances to Ralstonia solanacearum
Frontiers in Plant Science, 2014Co-Authors: Gaa Lle HuetAbstract:Ralstonia solanacearum is one of the most devastating bacterial plant pathogens due to its large host range, worldwide geographic distribution and persistence in fields. This soilborne pathogen is the causal agent of bacterial wilt and it can infect major agricultural crops thereby reducing significantly their yield. To favour infection, the bacterium delivers, through the type three secretion system, effectors that manipulate plant immunity. In this review, the relative efficiency of control strategies and existing resistances to R. solanacearum will be presented. Then, we will describe the genetic and molecular insights gained from the study of bacterial wilt in model plants. Finally, we will explore how the knowledge gathered from unravelling avirulence and virulence mechanisms of R. solanacearum effectors could help to develop more durable resistances in crop plants toward this destructive pathogen.
Huijun Wu - One of the best experts on this subject based on the ideXlab platform.
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bacillus volatiles adversely affect the physiology and ultra structure of Ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt
Scientific Reports, 2017Co-Authors: Hafiz Abdul Samad Tahir, Qin Gu, Huijun WuAbstract:Bacillus volatiles adversely affect the physiology and ultra-structure of Ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt
Dirk Hoffmeister - One of the best experts on this subject based on the ideXlab platform.
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ralfuranone thioether production by the plant pathogen Ralstonia solanacearum
ChemBioChem, 2013Co-Authors: Julia Pauly, Caitilyn Allen, Dieter Spiteller, Jeanine Linz, Jonathan M Jacobs, Markus Nett, Dirk HoffmeisterAbstract:: Ralfuranones are aryl-substituted furanone secondary metabolites of the Gram-negative plant pathogen Ralstonia solanacearum. New sulfur-containing ralfuranone derivatives were identified, including the methyl thioether-containing ralfuranone D. Isotopic labeling in vivo, as well as headspace analyses of volatiles from R. solanacearum liquid cultures, established a mechanism for the transfer of an intact methylthio group from L-methionine or α-keto-γ-methylthiobutyric acid. The methylthio acceptor molecule ralfuranone I, a previously postulated biosynthetic intermediate in ralfuranone biosynthesis, was isolated and characterized by NMR. The highly reactive Michael acceptor system of this intermediate readily reacts with various thiols, including glutathione.