Random Bred Mouse

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D G Whittingham - One of the best experts on this subject based on the ideXlab platform.

  • the dynamic provision of different energy substrates improves development of one cell Random Bred Mouse embryos in vitro
    Reproduction, 1992
    Co-Authors: J J G Brown, D G Whittingham
    Abstract:

    Preliminary observations showed that one-cell embryos from Random-Bred MF1 mice avoid cleavage arrest at the two-cell stage ('in vitro two-cell block') when cultured in modified M16 culture medium containing lactate and pyruvate but lacking glucose. The roles of lactate, pyruvate and glucose during preimplantation development of embryos from Random-Bred mice in vitro were therefore examined. When all three substrates were present continuously during culture, one-cell embryos arrested at the two- to four-cell stages. Improved development to the morula stage after 96 h in culture was obtained in media containing pyruvate alone, lactate and pyruvate, pyruvate and glucose, lactate pyruvate and glucose for the first 24 h, and medium containing lactate and pyruvate for the remaining 72 h. In a second experiment, embryos were cultured in medium containing pyruvate alone, lactate and pyruvate or pyruvate and glucose for the first 24 h, and lactate plus pyruvate medium for the second 24 h. Subsequent transfer to medium containing lactate, pyruvate and glucose supported the morula to blastocyst transition. These results show that developmental arrest in vitro can be overcome by changing the combination of energy substrates at different stages of preimplantation development.

Ikuo Kojima - One of the best experts on this subject based on the ideXlab platform.

  • effect of medium conditioned with rat hepatoma brl cells on 2 cell block of Random Bred Mouse embryos cultured in vitro
    Zygote, 2009
    Co-Authors: Masayuki Kobayashi, Yoshinori Terawaki, Koichi Saito, Kano Kasuga, Ikuo Kojima
    Abstract:

    The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain Mouse strains during in vitro culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 Mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr 10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the beta-A or beta-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.

J J G Brown - One of the best experts on this subject based on the ideXlab platform.

  • the dynamic provision of different energy substrates improves development of one cell Random Bred Mouse embryos in vitro
    Reproduction, 1992
    Co-Authors: J J G Brown, D G Whittingham
    Abstract:

    Preliminary observations showed that one-cell embryos from Random-Bred MF1 mice avoid cleavage arrest at the two-cell stage ('in vitro two-cell block') when cultured in modified M16 culture medium containing lactate and pyruvate but lacking glucose. The roles of lactate, pyruvate and glucose during preimplantation development of embryos from Random-Bred mice in vitro were therefore examined. When all three substrates were present continuously during culture, one-cell embryos arrested at the two- to four-cell stages. Improved development to the morula stage after 96 h in culture was obtained in media containing pyruvate alone, lactate and pyruvate, pyruvate and glucose, lactate pyruvate and glucose for the first 24 h, and medium containing lactate and pyruvate for the remaining 72 h. In a second experiment, embryos were cultured in medium containing pyruvate alone, lactate and pyruvate or pyruvate and glucose for the first 24 h, and lactate plus pyruvate medium for the second 24 h. Subsequent transfer to medium containing lactate, pyruvate and glucose supported the morula to blastocyst transition. These results show that developmental arrest in vitro can be overcome by changing the combination of energy substrates at different stages of preimplantation development.

Masayuki Kobayashi - One of the best experts on this subject based on the ideXlab platform.

  • effect of medium conditioned with rat hepatoma brl cells on 2 cell block of Random Bred Mouse embryos cultured in vitro
    Zygote, 2009
    Co-Authors: Masayuki Kobayashi, Yoshinori Terawaki, Koichi Saito, Kano Kasuga, Ikuo Kojima
    Abstract:

    The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain Mouse strains during in vitro culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 Mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr 10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the beta-A or beta-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.

Yoshinori Terawaki - One of the best experts on this subject based on the ideXlab platform.

  • effect of medium conditioned with rat hepatoma brl cells on 2 cell block of Random Bred Mouse embryos cultured in vitro
    Zygote, 2009
    Co-Authors: Masayuki Kobayashi, Yoshinori Terawaki, Koichi Saito, Kano Kasuga, Ikuo Kojima
    Abstract:

    The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain Mouse strains during in vitro culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 Mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr 10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the beta-A or beta-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.