The Experts below are selected from a list of 284457 Experts worldwide ranked by ideXlab platform
Yoichi Arai - One of the best experts on this subject based on the ideXlab platform.
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Novel Rat Model of stress urinary incontinence with a retroflexed bladder
International urogynecology journal, 2010Co-Authors: Naoki Kawamorita, Yasuhiro Kaiho, Haruo Nakagawa, Minoru Miyazato, Mabumi Matsushita, Yoichi AraiAbstract:Introduction and hypothesis We created a Rat Model with a retroflexed bladder that mimicked the loss of the posterior urethrovesical angle and compared the results with sham-surgery Rats for the establishment of Rat Models of stress urinary incontinence.
Yasser A. Khadrawy - One of the best experts on this subject based on the ideXlab platform.
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Effect of frankincense oil on the neurochemical changes induced in Rat Model of status epilepticus
Clinical Phytoscience, 2020Co-Authors: Eman N. Hosny, Mohamed E. Elhadidy, Hussein G. Sawie, Ayman Kilany, Yasser A. KhadrawyAbstract:Background The current objective is to evaluate the effect of frankincense oil on the convulsions and the associated neurochemical alteRations produced in pilocarpine-induced status epilepticus Rat Model. Methods Rats were divided randomly into: control, status epilepticus Rat Model and Rat Model of status epilepticus pretreated with frankincense oil daily for 5 days before pilocarpine treatment. On the fifth day, after pilocarpine injection, Rats were observed to evaluate the severity of seizures for 2 h. The oxidative stress parameters malondialdehyde, reduced glutathione and nitric oxide, the proinflammatory cytokines interleukin-6 and interleukin-1β and acetylcholinesterase were determined in the cortex, hippocampus and striatum. Dopamine, norepinephrine and serotonin were measured in the cortex and striatum. Results The status epilepticus Model exhibited repetitive seizures in the form of generalized tonic- clonic convulsions after 30 min. of pilocarpine injection. This was associated with a significant increase in the levels of malondialdehyde and nitric oxide and a significant decrease in reduced glutathione in the three regions. A significant increase was also observed in interleukin-1β, interleukin-6 and acetylcholinesterase. In the cortex and striatum, a significant decrease was recorded in monoamine levels. Pretreatment of Rat Model of status epilepticus with frankincense oil decreased the severity of seizures that appeared in the form of tremors and facial automatisms and prevented the increase in malondialdehyde, nitric oxide, interleukin-1β, interleukin-6 and acetylcholinesterase and the decrease in reduced glutathione induced by pilocarpine in the studied brain regions. Frankincense oil failed to restore the decreased level of cortical serotonin and dopamine. In the striatum, frankincense oil improved the levels of serotonin and norepinephrine but failed to restore the decreased dopamine levels. Conclusion It is clear from the present results that frankincense oil reduced the severity of seizures induced by pilocarpine. This could be mediated by its potent antioxidant and anti-inflammatory effects.
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Effect of frankincense oil on the neurochemical changes induced in Rat Model of status epilepticus
Clinical Phytoscience, 2020Co-Authors: Eman N. Hosny, Mohamed E. Elhadidy, Hussein G. Sawie, Ayman Kilany, Yasser A. KhadrawyAbstract:The current objective is to evaluate the effect of frankincense oil on the convulsions and the associated neurochemical alteRations produced in pilocarpine-induced status epilepticus Rat Model. Rats were divided randomly into: control, status epilepticus Rat Model and Rat Model of status epilepticus pretreated with frankincense oil daily for 5 days before pilocarpine treatment. On the fifth day, after pilocarpine injection, Rats were observed to evaluate the severity of seizures for 2 h. The oxidative stress parameters malondialdehyde, reduced glutathione and nitric oxide, the proinflammatory cytokines interleukin-6 and interleukin-1β and acetylcholinesterase were determined in the cortex, hippocampus and striatum. Dopamine, norepinephrine and serotonin were measured in the cortex and striatum. The status epilepticus Model exhibited repetitive seizures in the form of generalized tonic- clonic convulsions after 30 min. of pilocarpine injection. This was associated with a significant increase in the levels of malondialdehyde and nitric oxide and a significant decrease in reduced glutathione in the three regions. A significant increase was also observed in interleukin-1β, interleukin-6 and acetylcholinesterase. In the cortex and striatum, a significant decrease was recorded in monoamine levels. Pretreatment of Rat Model of status epilepticus with frankincense oil decreased the severity of seizures that appeared in the form of tremors and facial automatisms and prevented the increase in malondialdehyde, nitric oxide, interleukin-1β, interleukin-6 and acetylcholinesterase and the decrease in reduced glutathione induced by pilocarpine in the studied brain regions. Frankincense oil failed to restore the decreased level of cortical serotonin and dopamine. In the striatum, frankincense oil improved the levels of serotonin and norepinephrine but failed to restore the decreased dopamine levels. It is clear from the present results that frankincense oil reduced the severity of seizures induced by pilocarpine. This could be mediated by its potent antioxidant and anti-inflammatory effects.
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Assessment of the antidepressant effect of caffeine using Rat Model of depression induced by reserpine
SpringerOpen, 2018Co-Authors: Yasser A. Khadrawy, Eman N. Hosny, Hussein G. Sawie, Hagar H. MouradAbstract:Abstract Objective The present objective is to evaluate the antidepressant activity of caffeine. Material and methods Three groups of Rats were used; control, reserpine-induced Rat Model of depression, and Rat Model of depression treated daily with caffeine. At the end of the experiment, the motor activity of Rats was measured using open field test. On the next day, the animals of the three groups were sacrificed to measure levels of serotonin, norepinephrine, and dopamine in the cortex and hippocampus by spectrofluorometer. In addition, the levels of lipid peroxidation (MDA), nitric oxide (NO), and reduced glutathione (GSH) together with the activities of acetylcholinesterase (AchE) and Na+, K+, ATPase were measured in the two studied brain regions by spectrophotometer. Results In the Rat Model of depression, the animals showed a significant decrease in motor activity. This was associated with significant decreases in serotonin, norepinephrine, and dopamine in the cortex and hippocampus. However, significant increases in the activities of AchE and Na+, K+, ATPase, and the levels of MDA and NO were recorded in both areas of Rat Model of depression while GSH showed a significant decrease in the hippocampus. Caffeine failed to restore the decrease in motor activity. Caffeine treatment amelioRated the changes in cortical and hippocampal norepinephrine and dopamine and hippocampal serotonin. In addition, it restored MDA and GSH levels. However, it failed to prevent the increased AchE and Na+, K+, ATPase activities, and NO levels. Conclusions The present findings indicate that caffeine has a partial antidepressant effect mediated by its antioxidant activity and enhancement of monoamine levels
Naoki Kawamorita - One of the best experts on this subject based on the ideXlab platform.
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Novel Rat Model of stress urinary incontinence with a retroflexed bladder
International urogynecology journal, 2010Co-Authors: Naoki Kawamorita, Yasuhiro Kaiho, Haruo Nakagawa, Minoru Miyazato, Mabumi Matsushita, Yoichi AraiAbstract:Introduction and hypothesis We created a Rat Model with a retroflexed bladder that mimicked the loss of the posterior urethrovesical angle and compared the results with sham-surgery Rats for the establishment of Rat Models of stress urinary incontinence.
Eman N. Hosny - One of the best experts on this subject based on the ideXlab platform.
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Effect of frankincense oil on the neurochemical changes induced in Rat Model of status epilepticus
Clinical Phytoscience, 2020Co-Authors: Eman N. Hosny, Mohamed E. Elhadidy, Hussein G. Sawie, Ayman Kilany, Yasser A. KhadrawyAbstract:Background The current objective is to evaluate the effect of frankincense oil on the convulsions and the associated neurochemical alteRations produced in pilocarpine-induced status epilepticus Rat Model. Methods Rats were divided randomly into: control, status epilepticus Rat Model and Rat Model of status epilepticus pretreated with frankincense oil daily for 5 days before pilocarpine treatment. On the fifth day, after pilocarpine injection, Rats were observed to evaluate the severity of seizures for 2 h. The oxidative stress parameters malondialdehyde, reduced glutathione and nitric oxide, the proinflammatory cytokines interleukin-6 and interleukin-1β and acetylcholinesterase were determined in the cortex, hippocampus and striatum. Dopamine, norepinephrine and serotonin were measured in the cortex and striatum. Results The status epilepticus Model exhibited repetitive seizures in the form of generalized tonic- clonic convulsions after 30 min. of pilocarpine injection. This was associated with a significant increase in the levels of malondialdehyde and nitric oxide and a significant decrease in reduced glutathione in the three regions. A significant increase was also observed in interleukin-1β, interleukin-6 and acetylcholinesterase. In the cortex and striatum, a significant decrease was recorded in monoamine levels. Pretreatment of Rat Model of status epilepticus with frankincense oil decreased the severity of seizures that appeared in the form of tremors and facial automatisms and prevented the increase in malondialdehyde, nitric oxide, interleukin-1β, interleukin-6 and acetylcholinesterase and the decrease in reduced glutathione induced by pilocarpine in the studied brain regions. Frankincense oil failed to restore the decreased level of cortical serotonin and dopamine. In the striatum, frankincense oil improved the levels of serotonin and norepinephrine but failed to restore the decreased dopamine levels. Conclusion It is clear from the present results that frankincense oil reduced the severity of seizures induced by pilocarpine. This could be mediated by its potent antioxidant and anti-inflammatory effects.
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Effect of frankincense oil on the neurochemical changes induced in Rat Model of status epilepticus
Clinical Phytoscience, 2020Co-Authors: Eman N. Hosny, Mohamed E. Elhadidy, Hussein G. Sawie, Ayman Kilany, Yasser A. KhadrawyAbstract:The current objective is to evaluate the effect of frankincense oil on the convulsions and the associated neurochemical alteRations produced in pilocarpine-induced status epilepticus Rat Model. Rats were divided randomly into: control, status epilepticus Rat Model and Rat Model of status epilepticus pretreated with frankincense oil daily for 5 days before pilocarpine treatment. On the fifth day, after pilocarpine injection, Rats were observed to evaluate the severity of seizures for 2 h. The oxidative stress parameters malondialdehyde, reduced glutathione and nitric oxide, the proinflammatory cytokines interleukin-6 and interleukin-1β and acetylcholinesterase were determined in the cortex, hippocampus and striatum. Dopamine, norepinephrine and serotonin were measured in the cortex and striatum. The status epilepticus Model exhibited repetitive seizures in the form of generalized tonic- clonic convulsions after 30 min. of pilocarpine injection. This was associated with a significant increase in the levels of malondialdehyde and nitric oxide and a significant decrease in reduced glutathione in the three regions. A significant increase was also observed in interleukin-1β, interleukin-6 and acetylcholinesterase. In the cortex and striatum, a significant decrease was recorded in monoamine levels. Pretreatment of Rat Model of status epilepticus with frankincense oil decreased the severity of seizures that appeared in the form of tremors and facial automatisms and prevented the increase in malondialdehyde, nitric oxide, interleukin-1β, interleukin-6 and acetylcholinesterase and the decrease in reduced glutathione induced by pilocarpine in the studied brain regions. Frankincense oil failed to restore the decreased level of cortical serotonin and dopamine. In the striatum, frankincense oil improved the levels of serotonin and norepinephrine but failed to restore the decreased dopamine levels. It is clear from the present results that frankincense oil reduced the severity of seizures induced by pilocarpine. This could be mediated by its potent antioxidant and anti-inflammatory effects.
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Assessment of the antidepressant effect of caffeine using Rat Model of depression induced by reserpine
SpringerOpen, 2018Co-Authors: Yasser A. Khadrawy, Eman N. Hosny, Hussein G. Sawie, Hagar H. MouradAbstract:Abstract Objective The present objective is to evaluate the antidepressant activity of caffeine. Material and methods Three groups of Rats were used; control, reserpine-induced Rat Model of depression, and Rat Model of depression treated daily with caffeine. At the end of the experiment, the motor activity of Rats was measured using open field test. On the next day, the animals of the three groups were sacrificed to measure levels of serotonin, norepinephrine, and dopamine in the cortex and hippocampus by spectrofluorometer. In addition, the levels of lipid peroxidation (MDA), nitric oxide (NO), and reduced glutathione (GSH) together with the activities of acetylcholinesterase (AchE) and Na+, K+, ATPase were measured in the two studied brain regions by spectrophotometer. Results In the Rat Model of depression, the animals showed a significant decrease in motor activity. This was associated with significant decreases in serotonin, norepinephrine, and dopamine in the cortex and hippocampus. However, significant increases in the activities of AchE and Na+, K+, ATPase, and the levels of MDA and NO were recorded in both areas of Rat Model of depression while GSH showed a significant decrease in the hippocampus. Caffeine failed to restore the decrease in motor activity. Caffeine treatment amelioRated the changes in cortical and hippocampal norepinephrine and dopamine and hippocampal serotonin. In addition, it restored MDA and GSH levels. However, it failed to prevent the increased AchE and Na+, K+, ATPase activities, and NO levels. Conclusions The present findings indicate that caffeine has a partial antidepressant effect mediated by its antioxidant activity and enhancement of monoamine levels
Mabumi Matsushita - One of the best experts on this subject based on the ideXlab platform.
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Novel Rat Model of stress urinary incontinence with a retroflexed bladder
International urogynecology journal, 2010Co-Authors: Naoki Kawamorita, Yasuhiro Kaiho, Haruo Nakagawa, Minoru Miyazato, Mabumi Matsushita, Yoichi AraiAbstract:Introduction and hypothesis We created a Rat Model with a retroflexed bladder that mimicked the loss of the posterior urethrovesical angle and compared the results with sham-surgery Rats for the establishment of Rat Models of stress urinary incontinence.
