The Experts below are selected from a list of 24345 Experts worldwide ranked by ideXlab platform
Sonia Montanari - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a new inbred transgenic Rat Strain expressing dsred monomeric fluorescent protein
Transgenic Research, 2014Co-Authors: Eiji Kobayashi, Sonia Montanari, Xinghua Wang, Gustavo Yannarelli, Victor Dayan, Thorsten Berger, Larissa Zocche, Sowmya ViswanathanAbstract:The inbred Rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) Rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We geneRated and characterized a novel inbred Tg Lewis Rat Strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg Rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg Rat skin on wild-type Rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg Rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg Rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation.
Sowmya Viswanathan - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a new inbred transgenic Rat Strain expressing dsred monomeric fluorescent protein
Transgenic Research, 2014Co-Authors: Eiji Kobayashi, Sonia Montanari, Xinghua Wang, Gustavo Yannarelli, Victor Dayan, Thorsten Berger, Larissa Zocche, Sowmya ViswanathanAbstract:The inbred Rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) Rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We geneRated and characterized a novel inbred Tg Lewis Rat Strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg Rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg Rat skin on wild-type Rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg Rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg Rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation.
Arcady L Markel - One of the best experts on this subject based on the ideXlab platform.
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stress genes and hypertension contribution of the isiah Rat Strain study
Current Hypertension Reports, 2018Co-Authors: O E Redina, Arcady L MarkelAbstract:Acute psychoemotional stress is one of the causes of a sharp increase in blood pressure. However, the question if the stress may promote the hypertensive disease development is still open. This review aims, firstly, to show that the genetically determined enhanced responsiveness to stress is linked to sustained hypertension development and, secondly, to characterize the main physiological mechanisms and genetic factors implicated in the pathogenesis of stress-sensitive hypertension. Recent findings helped to characterize the main neuroendocrine mechanisms and the specificity of the genetic background contributing to the stress-sensitive hypertension development in the ISIAH Rats. The ISIAH Rat Strain, which is an original model of the stress-sensitive arterial hypertension, can be considered as “living” proof that the genetic predisposition to increased stress-reactivity can lead to the development of persistent stress-dependent arterial hypertension. The ISIAH Rat Strain is characterized by the genetically determined enhanced response of the neuroendocrine and renal regulatory systems to stress and is a suitable model that allows one to explore the genetic and physiological mechanisms involved in stress-sensitive hypertension development. There are common genetic loci (QTLs) associated with both basal and stress-induced blood pressure (BP) levels as well as QTLs associated with BP and other traits, which may be related to hypertension development in ISIAH Rats. Multiple genes differentially expressed in the target organs/tissues of hypertensive ISIAH and normotensive control Rats are associated with many biological processes and metabolic pathways involved in stress response and arterial hypertension. The genotype of ISIAH Rats is characterized by numerous specific and common SNPs as compared with other models of hypertensive Rats. The results of the studies are valuable for the search for genetic markers specific for stress-induced arterial hypertension, as well as for the selection of new molecular targets that may be potentially useful for prevention and/or therapy of hypertensive disease.
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hypotensive and neurometabolic effects of intragastric reishi ganoderma lucidum administRation in hypertensive isiah Rat Strain
Phytomedicine, 2018Co-Authors: Oleg B Shevelev, Arcady L Markel, A A Seryapina, E L Zavjalov, L A Gerlinskaya, Tatiana N Goryachkovskaya, Nikolay M Slynko, L V Kuibida, S E Peltek, M P MoshkinAbstract:Abstract Background As the standard clinically used hypotensive medicines have many undesirable side effects, there is a need for new therapeutic agents, especially ones of a natural origin. Purpose One possible candidate is extract from the mushroom Reishi (Ganoderma lucidum), which is used in the treatment and prevention of many chronic diseases. Study design and methods To study the effectiveness of Reishi, which grows in the Altai Mountains, as an antihypertensive agent, we intragastrically administered Reishi water extract to adult male hypertensive ISIAH (inherited stress-induced arterial hypertension) Rats. Results After seven weeks, Reishi therapy reduced blood pressure in experimental animals at a level comparable to that of losartan. Unlike losartan, intragastric Reishi introduction significantly increases cerebral blood flow and affects cerebral cortex metabolic patterns, shifting the balance of inhibitory and excitatory neurotransmitters toward excitation. Conclusion Changes in cerebral blood flow and Ratios of neurometabolites suggests Reishi has a potential nootropic effect.
C J Peters - One of the best experts on this subject based on the ideXlab platform.
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infection of inbred Rat Strains with rift valley fever virus development of a congenic resistant Strain and observations on age dependence of resistance
American Journal of Tropical Medicine and Hygiene, 1991Co-Authors: George W Anderson, Joseph A Rosebrock, Anthony J Johnson, Gerald B Jennings, C J PetersAbstract:Abstract A congenic Rat Strain (WF.LEW) was derived from the susceptible Wistar-Furth (WF) (background Strain) and the resistant LEW (donor Strain) inbred Strains and was used to evaluate the phenotypic expression of a dominant Mendelian gene that confers resistance to fatal hepatic disease caused by the ZH501 Strain of Rift Valley fever virus (RVFV). Resistance to hepatic disease developed gradually with age, with full expression at approximately 10 weeks in the WF.LEW and LEW Rat Strains. The ZH501 Strain caused fatal hepatitis in WF Rats regardless of age. However, resistance to the SA75 RVFV Strain (relatively non-pathogenic for adult Rats), was age- and dose-dependent in both WF and LEW Rats. The resistance gene transferred to the newly derived WF.LEW congenic Rat Strain appears to amplify age-dependent resistance of adult Rats, resulting in protection against fatal hepatic disease caused by the virulent ZH501 Strain. The congenic Rat Strain will be a valuable asset in elucidating the mechanism of resistance to Rift Valley fever virus governed by the dominant Mendelian gene.
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infection of inbred Rat Strains with rift valley fever virus development of a congenic resistant Strain and observations on age dependence of resistance
American Journal of Tropical Medicine and Hygiene, 1991Co-Authors: George W Anderson, Joseph A Rosebrock, Anthony J Johnson, Gerald B Jennings, C J PetersAbstract:Abstract A congenic Rat Strain (WF.LEW) was derived from the susceptible Wistar-Furth (WF) (background Strain) and the resistant LEW (donor Strain) inbred Strains and was used to evaluate the phenotypic expression of a dominant Mendelian gene that confers resistance to fatal hepatic disease caused by the ZH501 Strain of Rift Valley fever virus (RVFV). Resistance to hepatic disease developed gradually with age, with full expression at approximately 10 weeks in the WF.LEW and LEW Rat Strains. The ZH501 Strain caused fatal hepatitis in WF Rats regardless of age. However, resistance to the SA75 RVFV Strain (relatively non-pathogenic for adult Rats), was age- and dose-dependent in both WF and LEW Rats. The resistance gene transferred to the newly derived WF.LEW congenic Rat Strain appears to amplify age-dependent resistance of adult Rats, resulting in protection against fatal hepatic disease caused by the virulent ZH501 Strain. The congenic Rat Strain will be a valuable asset in elucidating the mechanism of resistance to Rift Valley fever virus governed by the dominant Mendelian gene.
George W Anderson - One of the best experts on this subject based on the ideXlab platform.
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infection of inbred Rat Strains with rift valley fever virus development of a congenic resistant Strain and observations on age dependence of resistance
American Journal of Tropical Medicine and Hygiene, 1991Co-Authors: George W Anderson, Joseph A Rosebrock, Anthony J Johnson, Gerald B Jennings, C J PetersAbstract:Abstract A congenic Rat Strain (WF.LEW) was derived from the susceptible Wistar-Furth (WF) (background Strain) and the resistant LEW (donor Strain) inbred Strains and was used to evaluate the phenotypic expression of a dominant Mendelian gene that confers resistance to fatal hepatic disease caused by the ZH501 Strain of Rift Valley fever virus (RVFV). Resistance to hepatic disease developed gradually with age, with full expression at approximately 10 weeks in the WF.LEW and LEW Rat Strains. The ZH501 Strain caused fatal hepatitis in WF Rats regardless of age. However, resistance to the SA75 RVFV Strain (relatively non-pathogenic for adult Rats), was age- and dose-dependent in both WF and LEW Rats. The resistance gene transferred to the newly derived WF.LEW congenic Rat Strain appears to amplify age-dependent resistance of adult Rats, resulting in protection against fatal hepatic disease caused by the virulent ZH501 Strain. The congenic Rat Strain will be a valuable asset in elucidating the mechanism of resistance to Rift Valley fever virus governed by the dominant Mendelian gene.
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infection of inbred Rat Strains with rift valley fever virus development of a congenic resistant Strain and observations on age dependence of resistance
American Journal of Tropical Medicine and Hygiene, 1991Co-Authors: George W Anderson, Joseph A Rosebrock, Anthony J Johnson, Gerald B Jennings, C J PetersAbstract:Abstract A congenic Rat Strain (WF.LEW) was derived from the susceptible Wistar-Furth (WF) (background Strain) and the resistant LEW (donor Strain) inbred Strains and was used to evaluate the phenotypic expression of a dominant Mendelian gene that confers resistance to fatal hepatic disease caused by the ZH501 Strain of Rift Valley fever virus (RVFV). Resistance to hepatic disease developed gradually with age, with full expression at approximately 10 weeks in the WF.LEW and LEW Rat Strains. The ZH501 Strain caused fatal hepatitis in WF Rats regardless of age. However, resistance to the SA75 RVFV Strain (relatively non-pathogenic for adult Rats), was age- and dose-dependent in both WF and LEW Rats. The resistance gene transferred to the newly derived WF.LEW congenic Rat Strain appears to amplify age-dependent resistance of adult Rats, resulting in protection against fatal hepatic disease caused by the virulent ZH501 Strain. The congenic Rat Strain will be a valuable asset in elucidating the mechanism of resistance to Rift Valley fever virus governed by the dominant Mendelian gene.
