The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform
Beverly Egyir - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana.
BMC microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The blaTEM gene was detected in 4% (4/98) of E. coli isolates in this study. The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana
BMC Microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Background Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Results Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm^2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm^2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The bla _ TEM gene was detected in 4% (4/98) of E. coli isolates in this study. Conclusion The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
Esther Dsani - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana.
BMC microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The blaTEM gene was detected in 4% (4/98) of E. coli isolates in this study. The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana
BMC Microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Background Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Results Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm^2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm^2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The bla _ TEM gene was detected in 4% (4/98) of E. coli isolates in this study. Conclusion The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
K. Muraleedharan - One of the best experts on this subject based on the ideXlab platform.
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Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of Raw Meat
International Journal of Biological Macromolecules, 2017Co-Authors: P. Mujeeb Rahman, V.m. Abdul Mujeeb, K. MuraleedharanAbstract:As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging. A facile, one pot procedure was adopted for the preparation of the C-ZnC films. In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO. The prepared films were found to be much superior when compared to bare chitosan and other conventional films. Two bacterial strains that commonly contaminate in packed Meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film. Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite. The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for Raw Meat. The prepared pouches showed significant action against the microbes in Raw Meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4 °C. The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of Raw Meat.
Basil Benduri Kaburi - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana.
BMC microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The blaTEM gene was detected in 4% (4/98) of E. coli isolates in this study. The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana
BMC Microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Background Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Results Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm^2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm^2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The bla _ TEM gene was detected in 4% (4/98) of E. coli isolates in this study. Conclusion The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
Ernest Kenu - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana.
BMC microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The blaTEM gene was detected in 4% (4/98) of E. coli isolates in this study. The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from Raw Meat in Greater Accra region, Ghana
BMC Microbiology, 2020Co-Authors: Esther Dsani, Edwin Afari, Anthony Danso-appiah, Ernest Kenu, Basil Benduri Kaburi, Beverly EgyirAbstract:Background Typically, Raw Meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of Meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of Raw Meat, the antimicrobial susceptibility and Extended Spectrum Beta Lactamase (ESBL) production in E. coli isolates from Raw Meat. Results Total Plate Counts exceeded the acceptable limit of 5.0 log CFU/ cm^2 in 60.5% (124/205) of Raw Meat samples. Total Coliform Counts in 70.7% (145/205) of samples were in excess of the acceptable limit of 2.5 log CFU/cm^2. E. coli was detected in about half of Raw Meat samples (48%), ranging from 9.5–79.0% among the slaughter sites. Isolates were susceptible to meropenem (100%), ceftriaxone (99%), cefotaxime (98%), chloramphenicol (97%), gentamycin (97%), ciprofloxacin (92%) and amikacin (92%), but resistant to ampicillin (57%), tetracycline (45%), sulfamethoxazole-trimethoprim (21%) and cefuroxime (17%). Multi-drug resistance (MDR) was identified in 22% of the isolates. The bla _ TEM gene was detected in 4% (4/98) of E. coli isolates in this study. Conclusion The levels of microbial contamination of Raw Meat in this study were unacceptable. Meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli that are resistant to most antibiotics in use. We recommend an enhanced surveillance for antibiotic resistance in food products for the early detection of emerging resistant bacteria species in the food chain.
