The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
K L Johnson - One of the best experts on this subject based on the ideXlab platform.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions.
The Journal of allergy and clinical immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:The role of T Lymphocytes in mediating drug eruptions is uncertain. Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions☆☆☆★★★
Journal of Allergy and Clinical Immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:Abstract Background: The role of T Lymphocytes in mediating drug eruptions is uncertain. Methods: Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. Results: The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Conclusions: Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole. (J ALLERGY CLIN IMMUNOL 1994;94:465-72.)
R S Kalish - One of the best experts on this subject based on the ideXlab platform.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions.
The Journal of allergy and clinical immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:The role of T Lymphocytes in mediating drug eruptions is uncertain. Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions☆☆☆★★★
Journal of Allergy and Clinical Immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:Abstract Background: The role of T Lymphocytes in mediating drug eruptions is uncertain. Methods: Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. Results: The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Conclusions: Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole. (J ALLERGY CLIN IMMUNOL 1994;94:465-72.)
J A Wood - One of the best experts on this subject based on the ideXlab platform.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions.
The Journal of allergy and clinical immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:The role of T Lymphocytes in mediating drug eruptions is uncertain. Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions☆☆☆★★★
Journal of Allergy and Clinical Immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:Abstract Background: The role of T Lymphocytes in mediating drug eruptions is uncertain. Methods: Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. Results: The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Conclusions: Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole. (J ALLERGY CLIN IMMUNOL 1994;94:465-72.)
A Laporte - One of the best experts on this subject based on the ideXlab platform.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions.
The Journal of allergy and clinical immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:The role of T Lymphocytes in mediating drug eruptions is uncertain. Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole.
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Sulfonamide-Reactive Lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions☆☆☆★★★
Journal of Allergy and Clinical Immunology, 1994Co-Authors: R S Kalish, A Laporte, J A Wood, K L JohnsonAbstract:Abstract Background: The role of T Lymphocytes in mediating drug eruptions is uncertain. Methods: Twenty-four patients with eruptions induced by sulfonamide-related drugs were studied to detect Lymphocyte reactivity to drugs. Both the Lymphocyte transformation test and limiting dilution analysis were used as assays for drug-Reactive Lymphocytes. Peripheral blood Lymphocytes were expanded in interleukin-2 and tested for reactivity to sulfamethoxazole and furosemide. Results: The Lymphocyte transformation test results to sulfamethoxazole, sulfisoxazole, and furosemide were found to be generally unreliable with a high rate of false-negative and false-positive results. However, as determined by limiting dilution analysis, sulfamethoxazole-Reactive Lymphocytes were detected in the peripheral blood of one patient at a frequency of 1/172,000. This is within the lower range of frequencies of urushiol-Reactive T cells in the peripheral blood of patients with allergic contact dermatitis to urushiol (poison ivy). Two sulfonamide-Reactive Lymphocyte lines were cultured from two patients. Both lines proliferated in response to sulfamethoxazole but not in response to furosemide, suggesting that furosemide does not cross-react with the sulfonamides. Conclusions: Lymphocytes Reactive to sulfamethoxazole were detected at low frequencies in the peripheral blood of three patients with drug eruptions secondary to administration of sulfamethoxazole. (J ALLERGY CLIN IMMUNOL 1994;94:465-72.)
Alexander Scheffold - One of the best experts on this subject based on the ideXlab platform.
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Mould-Reactive T cells for the diagnosis of invasive mould infection-A prospective study.
Mycoses, 2019Co-Authors: Angela Steinbach, Oliver A. Cornely, Hilmar Wisplinghoff, Astrid Schauss, Joerg J. Vehreschild, Jan Rybniker, Axel Hamprecht, Anne Richter, Petra Bacher, Alexander ScheffoldAbstract:Invasive mould infections (IMI) in immunocompromised patients are difficult to diagnose. Early and targeted treatment is paramount, but minimally invasive tests reliably identifying pathogens are lacking. We previously showed that monitoring pathogen-specific CD4+T cells in peripheral blood using upregulation of induced CD154 positive Lymphocytes can be used to diagnose acute IMI. Here, we validate our findings in an independent patient cohort. We stimulated peripheral blood cells from at-risk patients with Aspergillus spp. and Mucorales lysates and quantitated mould-Reactive CD4/CD69/CD154 positive Lymphocytes via flow cytometry. Mould-Reactive Lymphocytes were quantitated in 115 at-risk patients. In 38 (33%) patients, the test was not evaluable, mainly due to low T cell counts or non-Reactive positive control. Test results were evaluable in 77 (67%) patients. Of these, four patients (5%) had proven IMI and elevated mould-Reactive T cell signals. Of 73 (95%) patients without proven IMI, 59 (81%) had mould-Reactive T cell signals within normal range. Fourteen (19%) patients without confirmed IMI showed elevated T cell signals and 11 of those received antifungal treatment. The mould-Reactive Lymphocyte assay identified presence of IMI with a sensitivity of 100% and specificity of 81%. The mould-Reactive Lymphocyte assay correctly identified all patients with proven IMI. Assay applicability is limited by low T cell counts during bone marrow suppression. The assay has the potential to support diagnosis of invasive mould infection to facilitate tailored treatment even when biopsies are contraindicated or cultures remain negative.
