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Kjeld Hermansen - One of the best experts on this subject based on the ideXlab platform.
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RebAudioside A directly stimulAtes insulin secretion from pAncreAtic betA cells: A glucose-dependent Action viA inhibition of ATP-sensitive K-chAnnels.
Diabetes obesity & metabolism, 2008Co-Authors: Reziwanggu Abudula, Per Bendix Jeppesen, Vladimir V. Matchkov, Holger Nilsson, Christian Aalkjaer, Kjeld HermansenAbstract:Recently, we showed thAt RebAudioside A potently stimulAtes the insulin secretion from isolAted mouse islets in A dose-, glucose- And CA(2+)-dependent mAnner. Little is known About the mechAnisms underlying the insulinotropic Action of RebAudioside A. The Aim of this study wAs to define the signAlling system by which, RebAudioside A Acts. IsolAted mouse islets were used in the cAMP[(125)I] scintillAtion proximity AssAy to meAsure totAl cAMP level, And in A luminometric method to meAsure intrAcellulAr ATP And ADP concentrAtions. ConventionAl And permeAbilized whole-cell configurAtion of the pAtch-clAmp technique wAs used to verify the effect of RebAudioside A on ATP-sensitive K(+)-chAnnels from dispersed single betA cells from isolAted mouse islets. Insulin wAs meAsured by rAdioimmunoAssAy from insulinomA MIN6 cells. In the presence of 16.7 mM glucose, the Addition of the mAximAlly effective concentrAtion of RebAudioside A (10(-9) M) increAsed the ATP/ADP rAtio significAntly, while it did not chAnge the intrAcellulAr cAMP level. RebAudioside A (10(-9) M) And stevioside (10(-6) M) reduced the ATP-sensitive potAssium chAnnel (K(ATP)) conductAnce in A glucose-dependent mAnner. Moreover, RebAudioside A stimulAted the insulin secretion from MIN6 cells in A dose- And glucose-dependent mAnner. In conclusion, the insulinotropic effect of RebAudioside A is mediAted viA inhibition of ATP-sensitive K(+)-chAnnels And requires the presence of high glucose. The inhibition of ATP-sensitive K(+)-chAnnels is probAbly induced by chAnges in the ATP/ADP rAtio. The results indicAte thAt RebAudioside A mAy offer A distinct therApeutic AdvAntAge over sulphonylureAs becAuse of less risk of cAusing hypoglycAemiA.
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the diterpene glycoside RebAudioside A does not improve glycemic control or Affect blood pressure After eight weeks treAtment in the goto kAkizAki rAt
The review of diabetic studies : RDS, 2005Co-Authors: Stig Eric Dyrskog, Per Bendix Jeppesen, J. W. Chen, Lars Porskjaer Christensen, Kjeld HermansenAbstract:The plAnt, SteviA rebAudiAnA Bertoni (SrB), hAs been used for the treAtment of diAbetes in trAditionAl medicine. Previously, we hAve demonstrAted thAt long-term AdministrAtion of the glycoside stevioside hAs insulinotropic, glucAgonostAtic, Anti-hyperglycemic And blood pressure-lowering effects in type 2 diAbetic AnimAl models. The Aim of this study wAs to elucidAte if long-term AdministrAtion of RebAudioside A, Another glycoside isolAted from the plAnt SrB, could improve glycemic control And lower blood pressure in An AnimAl model of type 2 diAbetes. We divided mAle Goto-KAkizAki (GK) rAts into two groups which were fed A stAndArd lAborAtory chow diet for eight weeks. The diet wAs supplemented with orAl RebAudioside A (0.025 g/kg BW/dAy) in the experimentAl group. Blood glucose, weight, blood pressure And food intAke were meAsured weekly. AnimAls were equipped with An intrA-ArteriAl cAtheter, And At week eight the conscious rAts underwent An intrA-ArteriAl glucose tolerAnce test (IAGTT) (2.0 g/kg BW). During the IAGTT, the level of glucose, glucAgon, And insulin responses did not differ significAntly between the two groups. FAsting levels of glucose, glucAgon, insulin or levels of blood lipids did not differ between the groups throughout the study period. We observed no effect on blood pressure or weight development. In conclusion, orAl supplementAtion with RebAudioside A (0.025 g/kg BW/dAy) for eight weeks did not influence blood pressure or glycemic control in GK rAts. RebAudioside A fAiled to show the beneficiAl effects in diAbetic AnimAls previously demonstrAted for stevioside.
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RebAudioside A potently stimulAtes insulin secretion from isolAted mouse islets: studies on the dose-, glucose-, And cAlcium-dependency.
Metabolism: clinical and experimental, 2004Co-Authors: Reziwanggu Abudula, Per Bendix Jeppesen, Stig Eric D. Rolfsen, Jianzhong Xiao, Kjeld HermansenAbstract:ExtrActs of leAves of the plAnt SteviA rebAudiAnA Bertoni (SrB), hAve been used for mAny yeArs in trAditionAl treAtment of diAbetes in South AmericA. SteviA leAves contAin diterpene glycosides, stevioside And RebAudioside A being the most AbundAnt. Recently, it wAs demonstrAted thAt stevioside stimulAtes the insulin secretion both in vitro And in vivo. Subsequently, we wAnted to elucidAte the influence of RebAudioside A on the insulin releAse from mouse islets using stAtic incubAtions, As well As perifusion experiments. RebAudioside A (10(-16) to 10(-6) mol/L) dose-dependently stimulAted the insulin secretion in the presence of 16.7 mmol/L glucose (P 6.6 mmol/L. The effect of RebAudioside A is criticAlly dependent on the presence of extrAcellulAr CA2+, ie, RebAudioside A-induced insulin stimulAtion At high glucose disAppeArs in the Absence of extrAcellulAr CA2+. In conclusion, RebAudioside A possesses insulinotropic effects And mAy serve A potentiAl role As treAtment in type 2 diAbetes mellitus.
Andreas Wawrzun - One of the best experts on this subject based on the ideXlab platform.
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investigAtions on the stAbility of stevioside And RebAudioside A in soft drinks
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Ursula Wolwerrieck, Werner Tomberg, Andreas WawrzunAbstract:The stAbility of the two steviol glycosides stevioside And RebAudioside A And the possible formAtion of the Aglycon steviol in different soft drinks were AnAlyzed in sAmples spiked with stevioside or RebAudioside A After 24, 48, And 72 h storAge times At 80 °C. DegrAdAtion of up to 70% wAs observed, And stevioside wAs less stAble thAn RebAudioside A. Stevioside And RebAudioside A And their degrAdAtion products were AnAlyzed by high-performAnce liquid chromAtogrAphy with ultrAviolet detection (UV-HPLC) on A HILIC AnAlyticAl column, And the identity of the degrAdAtion products wAs confirmed by liquid chromAtogrAphy−electrosprAy ionizAtion mAss spectrometry (LC-ESI-MSn) in negAtive mode. A UV-HPLC method wAs developed using A C18 AnAlyticAl column to exclude the presence of the Aglycon steviol, which gAve A positive response in the forwArd mutAtion AssAy using the sensitive SAlmonellA typhimurium TM677 strAin. The recoveries of steviol with this method rAnged from 95.9 to 109.2%, And the cAlibrAtion curves w...
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investigAtions on the stAbility of stevioside And RebAudioside A in soft drinks
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Ursula Wolwerrieck, Werner Tomberg, Andreas WawrzunAbstract:The stAbility of the two steviol glycosides stevioside And RebAudioside A And the possible formAtion of the Aglycon steviol in different soft drinks were AnAlyzed in sAmples spiked with stevioside or RebAudioside A After 24, 48, And 72 h storAge times At 80 °C. DegrAdAtion of up to 70% wAs observed, And stevioside wAs less stAble thAn RebAudioside A. Stevioside And RebAudioside A And their degrAdAtion products were AnAlyzed by high-performAnce liquid chromAtogrAphy with ultrAviolet detection (UV-HPLC) on A HILIC AnAlyticAl column, And the identity of the degrAdAtion products wAs confirmed by liquid chromAtogrAphy−electrosprAy ionizAtion mAss spectrometry (LC-ESI-MSn) in negAtive mode. A UV-HPLC method wAs developed using A C18 AnAlyticAl column to exclude the presence of the Aglycon steviol, which gAve A positive response in the forwArd mutAtion AssAy using the sensitive SAlmonellA typhimurium TM677 strAin. The recoveries of steviol with this method rAnged from 95.9 to 109.2%, And the cAlibrAtion curves w...
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InvestigAtions on the stAbility of stevioside And RebAudioside A in soft drinks.
Journal of agricultural and food chemistry, 2010Co-Authors: Ursula Wölwer-rieck, Werner Tomberg, Andreas WawrzunAbstract:The stAbility of the two steviol glycosides stevioside And RebAudioside A And the possible formAtion of the Aglycon steviol in different soft drinks were AnAlyzed in sAmples spiked with stevioside or RebAudioside A After 24, 48, And 72 h storAge times At 80 °C. DegrAdAtion of up to 70% wAs observed, And stevioside wAs less stAble thAn RebAudioside A. Stevioside And RebAudioside A And their degrAdAtion products were AnAlyzed by high-performAnce liquid chromAtogrAphy with ultrAviolet detection (UV-HPLC) on A HILIC AnAlyticAl column, And the identity of the degrAdAtion products wAs confirmed by liquid chromAtogrAphy-electrosprAy ionizAtion mAss spectrometry (LC-ESI-MS(n)) in negAtive mode. A UV-HPLC method wAs developed using A C18 AnAlyticAl column to exclude the presence of the Aglycon steviol, which gAve A positive response in the forwArd mutAtion AssAy using the sensitive SAlmonellA typhimurium TM677 strAin. The recoveries of steviol with this method rAnged from 95.9 to 109.2%, And the cAlibrAtion curves were lineAr from 1 to 100 μg/mL with R(2) = 0.9999. The limit of detection wAs 1 μg/mL. ConfirmAtion by LC-ESI-MS(n) resulted in A LOD of 6 ng/mL. The Absence of steviol in the degrAded sAmples could be unAmbiguously confirmed by UV-HPLC And by LC-ESI-MS(n).
Weidong Yan - One of the best experts on this subject based on the ideXlab platform.
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Solid–Liquid Equilibrium of RebAudioside A in Pure And BinAry Mixed Solvents At T = (288.15 to 328.15) K
Journal of Chemical & Engineering Data, 2018Co-Authors: Behnaz Asadzadeh, Jialun Zhong, Weidong YanAbstract:Solid–liquid equilibrium dAtA of RebAudioside A (A nAturAl sweetener) in nine pure solvents (methAnol, ethAnol, 1-propAnol, 2-propAnol, Acetonitrile, 2-methoxyethAnol, 2-ethoxyethAnol, 2-buthoxyeth...
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Solid–Liquid Equilibrium of RebAudioside A in Pure And BinAry Mixed Solvents At T = (288.15 to 328.15) K
2018Co-Authors: Behnaz Asadzadeh, Jialun Zhong, Weidong YanAbstract:Solid–liquid equilibrium dAtA of RebAudioside A (A nAturAl sweetener) in nine pure solvents (methAnol, ethAnol, 1-propAnol, 2-propAnol, Acetonitrile, 2-methoxyethAnol, 2-ethoxyethAnol, 2-buthoxyethAnol, And ethylene glycol) And two binAry mixed solvents (methAnol + ethAnol And 2-methoxyethAnol + ethAnol) were been meAsured by high performAnce liquid chromAtogrAphy At temperAtures rAnging from (288.15 to 328.15) K And Atmospheric pressure. From the experimentAl dAtA, it wAs found thAt the solubility increAsed with the rising temperAture in All studied solvents. The obtAined vAlues for the solubility of RebAudioside A in pure And mixed binAry solvents were correlAted with modified ApelblAt And nonrAndom two-liquid models. The results showed thAt these models cAn AccurAtely correlAte the solubility dAtA obtAined in this work. Furthermore, the crystAl hAbits of RebAudioside A in selected solvents were studied
Reziwanggu Abudula - One of the best experts on this subject based on the ideXlab platform.
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RebAudioside A directly stimulAtes insulin secretion from pAncreAtic betA cells: A glucose-dependent Action viA inhibition of ATP-sensitive K-chAnnels.
Diabetes obesity & metabolism, 2008Co-Authors: Reziwanggu Abudula, Per Bendix Jeppesen, Vladimir V. Matchkov, Holger Nilsson, Christian Aalkjaer, Kjeld HermansenAbstract:Recently, we showed thAt RebAudioside A potently stimulAtes the insulin secretion from isolAted mouse islets in A dose-, glucose- And CA(2+)-dependent mAnner. Little is known About the mechAnisms underlying the insulinotropic Action of RebAudioside A. The Aim of this study wAs to define the signAlling system by which, RebAudioside A Acts. IsolAted mouse islets were used in the cAMP[(125)I] scintillAtion proximity AssAy to meAsure totAl cAMP level, And in A luminometric method to meAsure intrAcellulAr ATP And ADP concentrAtions. ConventionAl And permeAbilized whole-cell configurAtion of the pAtch-clAmp technique wAs used to verify the effect of RebAudioside A on ATP-sensitive K(+)-chAnnels from dispersed single betA cells from isolAted mouse islets. Insulin wAs meAsured by rAdioimmunoAssAy from insulinomA MIN6 cells. In the presence of 16.7 mM glucose, the Addition of the mAximAlly effective concentrAtion of RebAudioside A (10(-9) M) increAsed the ATP/ADP rAtio significAntly, while it did not chAnge the intrAcellulAr cAMP level. RebAudioside A (10(-9) M) And stevioside (10(-6) M) reduced the ATP-sensitive potAssium chAnnel (K(ATP)) conductAnce in A glucose-dependent mAnner. Moreover, RebAudioside A stimulAted the insulin secretion from MIN6 cells in A dose- And glucose-dependent mAnner. In conclusion, the insulinotropic effect of RebAudioside A is mediAted viA inhibition of ATP-sensitive K(+)-chAnnels And requires the presence of high glucose. The inhibition of ATP-sensitive K(+)-chAnnels is probAbly induced by chAnges in the ATP/ADP rAtio. The results indicAte thAt RebAudioside A mAy offer A distinct therApeutic AdvAntAge over sulphonylureAs becAuse of less risk of cAusing hypoglycAemiA.
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RebAudioside A potently stimulAtes insulin secretion from isolAted mouse islets: studies on the dose-, glucose-, And cAlcium-dependency.
Metabolism: clinical and experimental, 2004Co-Authors: Reziwanggu Abudula, Per Bendix Jeppesen, Stig Eric D. Rolfsen, Jianzhong Xiao, Kjeld HermansenAbstract:ExtrActs of leAves of the plAnt SteviA rebAudiAnA Bertoni (SrB), hAve been used for mAny yeArs in trAditionAl treAtment of diAbetes in South AmericA. SteviA leAves contAin diterpene glycosides, stevioside And RebAudioside A being the most AbundAnt. Recently, it wAs demonstrAted thAt stevioside stimulAtes the insulin secretion both in vitro And in vivo. Subsequently, we wAnted to elucidAte the influence of RebAudioside A on the insulin releAse from mouse islets using stAtic incubAtions, As well As perifusion experiments. RebAudioside A (10(-16) to 10(-6) mol/L) dose-dependently stimulAted the insulin secretion in the presence of 16.7 mmol/L glucose (P 6.6 mmol/L. The effect of RebAudioside A is criticAlly dependent on the presence of extrAcellulAr CA2+, ie, RebAudioside A-induced insulin stimulAtion At high glucose disAppeArs in the Absence of extrAcellulAr CA2+. In conclusion, RebAudioside A possesses insulinotropic effects And mAy serve A potentiAl role As treAtment in type 2 diAbetes mellitus.
Ali Ahmad - One of the best experts on this subject based on the ideXlab platform.
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In vitro evAluAtion of the cAriogenic potentiAl of RebAudioside A compAred to sucrose And xylitol
Clinical Oral Investigations, 2019Co-Authors: Johanna Ganter, Sam Doerken, Elmar Hellwig, Ali AhmadAbstract:ObjectivesThe Aim of the present study wAs to evAluAte the growth And Acid synthesis of cAriogenic pAthogens in vitro when RebAudioside A wAs the sole source of cArbon, compAred to sucrose, xylitol, And A commerciAl sweetener contAining RebAudioside A.MAteriAls And methodsFirst, sucrose or sugAr substitutes were Added to humAn sAlivA And the pH wAs meAsured over 10 h. Subsequently, the growth And Acid synthesis efficiency of Streptococcus mutAns , Streptococcus sobrinus , Streptococcus orAlis , LActobAcillus rhAmnosus , LActobAcillus pArAcAsei , And CAndidA AlbicAns were investigAted: After Adding them to minimAl culture mediA contAining only one of the cArbon sources, the opticAl density And pH were determined over 10 h.ResultsA significAnt pH drop only occurred in the sucrose group of humAn sAlivA whereAs no differences were found between the sugAr substitute groups. The individuAl strAins of cAries pAthogens showed significAntly lower growth rAtes And less Acid synthesis in the sugAr replAcement groups, in compArison to sucrose. However, these groups Also showed bActeriAl growth And A decline in pH.ConclusionXylitol AppeAred to be superior to RebAudioside A regArding the inhibition of mutAns streptococci. The results for the remAining microorgAnisms indicAte thAt RebAudioside A cAuses A similAr or lower Acid synthesis thAn xylitol. As sAlivA contAins buffer systems, the metAbolism of RebAudioside A did not seem to shift the equilibrium to deminerAlizAtion.ClinicAl relevAnceRebAudioside A mAy not be suitAble As A tArgeted Streptococcus mutAns treAtment, but it could contribute to A reduction of cAries risk As A replAcement for sucrose.
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In vitro evAluAtion of the cAriogenic potentiAl of RebAudioside A compAred to sucrose And xylitol.
Clinical oral investigations, 2019Co-Authors: Johanna Ganter, Sam Doerken, Elmar Hellwig, Ali AhmadAbstract:The Aim of the present study wAs to evAluAte the growth And Acid synthesis of cAriogenic pAthogens in vitro when RebAudioside A wAs the sole source of cArbon, compAred to sucrose, xylitol, And A commerciAl sweetener contAining RebAudioside A. First, sucrose or sugAr substitutes were Added to humAn sAlivA And the pH wAs meAsured over 10 h. Subsequently, the growth And Acid synthesis efficiency of Streptococcus mutAns, Streptococcus sobrinus, Streptococcus orAlis, LActobAcillus rhAmnosus, LActobAcillus pArAcAsei, And CAndidA AlbicAns were investigAted: After Adding them to minimAl culture mediA contAining only one of the cArbon sources, the opticAl density And pH were determined over 10 h. A significAnt pH drop only occurred in the sucrose group of humAn sAlivA whereAs no differences were found between the sugAr substitute groups. The individuAl strAins of cAries pAthogens showed significAntly lower growth rAtes And less Acid synthesis in the sugAr replAcement groups, in compArison to sucrose. However, these groups Also showed bActeriAl growth And A decline in pH. Xylitol AppeAred to be superior to RebAudioside A regArding the inhibition of mutAns streptococci. The results for the remAining microorgAnisms indicAte thAt RebAudioside A cAuses A similAr or lower Acid synthesis thAn xylitol. As sAlivA contAins buffer systems, the metAbolism of RebAudioside A did not seem to shift the equilibrium to deminerAlizAtion. RebAudioside A mAy not be suitAble As A tArgeted Streptococcus mutAns treAtment, but it could contribute to A reduction of cAries risk As A replAcement for sucrose.
