The Experts below are selected from a list of 1815 Experts worldwide ranked by ideXlab platform
B Nock - One of the best experts on this subject based on the ideXlab platform.
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of Neuroscience Methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:Abstract A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of neuroscience methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of less than 1% and 6%, respectively. The assay was used to evaluate several potential methods for removing digitonin from biological samples (digitonin extracts from guinea pig brain membranes). Dialysis and G-25 Sephadex chromatography were ineffective. However, protein and digitonin can be effectively separated by ammonium sulfate precipitation followed by dialysis. The kappa 1 opioid Receptor survived these procedures with no change in affinity for [3H]U-69,593. In conclusion, the hemolytic assay for digitonin appears to provide a practical means for determining detergent concentrations during Receptor purification and characterization and for evaluating potential methods for detergent removal. Although an in depth analysis of the assay was carried out only for digitonin, CHAPS and deoxycholate also caused 50% hemolysis at concentrations well below those commonly used for Receptor Solubilization and, therefore, the general assay procedures might have applicability for measurement of these and perhaps other detergents used in Receptor Solubilization as well.
B W Moore - One of the best experts on this subject based on the ideXlab platform.
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of Neuroscience Methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:Abstract A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of neuroscience methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of less than 1% and 6%, respectively. The assay was used to evaluate several potential methods for removing digitonin from biological samples (digitonin extracts from guinea pig brain membranes). Dialysis and G-25 Sephadex chromatography were ineffective. However, protein and digitonin can be effectively separated by ammonium sulfate precipitation followed by dialysis. The kappa 1 opioid Receptor survived these procedures with no change in affinity for [3H]U-69,593. In conclusion, the hemolytic assay for digitonin appears to provide a practical means for determining detergent concentrations during Receptor purification and characterization and for evaluating potential methods for detergent removal. Although an in depth analysis of the assay was carried out only for digitonin, CHAPS and deoxycholate also caused 50% hemolysis at concentrations well below those commonly used for Receptor Solubilization and, therefore, the general assay procedures might have applicability for measurement of these and perhaps other detergents used in Receptor Solubilization as well.
A L Giordano - One of the best experts on this subject based on the ideXlab platform.
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of Neuroscience Methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:Abstract A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of neuroscience methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of less than 1% and 6%, respectively. The assay was used to evaluate several potential methods for removing digitonin from biological samples (digitonin extracts from guinea pig brain membranes). Dialysis and G-25 Sephadex chromatography were ineffective. However, protein and digitonin can be effectively separated by ammonium sulfate precipitation followed by dialysis. The kappa 1 opioid Receptor survived these procedures with no change in affinity for [3H]U-69,593. In conclusion, the hemolytic assay for digitonin appears to provide a practical means for determining detergent concentrations during Receptor purification and characterization and for evaluating potential methods for detergent removal. Although an in depth analysis of the assay was carried out only for digitonin, CHAPS and deoxycholate also caused 50% hemolysis at concentrations well below those commonly used for Receptor Solubilization and, therefore, the general assay procedures might have applicability for measurement of these and perhaps other detergents used in Receptor Solubilization as well.
M Bruckner - One of the best experts on this subject based on the ideXlab platform.
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of Neuroscience Methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:Abstract A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of
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A simple, highly sensitive assay for measurement of digitonin during Receptor Solubilization.
Journal of neuroscience methods, 1992Co-Authors: B W Moore, A L Giordano, M Bruckner, B NockAbstract:A simple and highly sensitive assay for measuring total digitonin in biological samples is described. The assay is based on the ability of digitonin to hemolyze red blood cells. The precision and reproducibility of the assay was excellent with intra- and inter-assay variabilities of less than 1% and 6%, respectively. The assay was used to evaluate several potential methods for removing digitonin from biological samples (digitonin extracts from guinea pig brain membranes). Dialysis and G-25 Sephadex chromatography were ineffective. However, protein and digitonin can be effectively separated by ammonium sulfate precipitation followed by dialysis. The kappa 1 opioid Receptor survived these procedures with no change in affinity for [3H]U-69,593. In conclusion, the hemolytic assay for digitonin appears to provide a practical means for determining detergent concentrations during Receptor purification and characterization and for evaluating potential methods for detergent removal. Although an in depth analysis of the assay was carried out only for digitonin, CHAPS and deoxycholate also caused 50% hemolysis at concentrations well below those commonly used for Receptor Solubilization and, therefore, the general assay procedures might have applicability for measurement of these and perhaps other detergents used in Receptor Solubilization as well.
Stephen P. Watson - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Ins(1,4,5)P3 Receptors from rat cerebellum and bovine adrenal cortex.
Biochimica et biophysica acta, 1993Co-Authors: Alison M. White, Mark A. Varney, Nobuaki Maeda, Katsuhiko Mikoshiba, Stephen P. WatsonAbstract:Abstract Ins(1,4,5)P 3 Receptors in adrenal cortical and cerebellar membranes can be distinguished by their affinities for Ins(1,4,5)P 3 as well as the potencies with which heparin and Mg 2+ inhibit binding. We have found that the differences in Ins(1,4,5)P 3 affinity and heparin inhibition are maintained upon Receptor Solubilization and purification. In contrast to this, heparin-agarose affinity purification of solubilized cerebellar Receptors reduces the potency of Mg 2+ inhibition to that in adrenal cortex. These results suggest that Ins(1,4,5)P 3 Receptors in adrenal cortex are structurally distinct from those in cerebellum. Monoclonal antibodies raised against C- and N-terminal regions of mouse cerebellar Ins(1,4,5)P 3 Receptors recognize 250–300-kDa proteins in both rat cerebellum and bovine adrenal cortex.
