The Experts below are selected from a list of 7860 Experts worldwide ranked by ideXlab platform
Jose Carlos Rodriguezcabello - One of the best experts on this subject based on the ideXlab platform.
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Recombinant DNA Technology and click chemistry a powerful combination for generating a hybrid elastin like statherin hydrogel to control calcium phosphate mineralization
Beilstein Journal of Nanotechnology, 2017Co-Authors: Mohamed Hamed Misbah, Mercedes Santos, Luis G Quintanilla, Christina Gunter, Matilde Alonso, Andreas Taubert, Jose Carlos RodriguezcabelloAbstract:Understanding the mechanisms responsible for generating different phases and morphologies of calcium phosphate by elastin-like recombinamers is supreme for bioengineering of advanced multifunctional materials. The generation of such multifunctional hybrid materials depends on the properties of their counterparts and the way in which they are assembled. The success of this assembly depends on the different approaches used, such as Recombinant DNA Technology and click chemistry. In the present work, an elastin-like recombinamer bearing lysine amino acids distributed along the recombinamer chain has been cross-linked via Huisgen [2 + 3] cycloaddition. The recombinamer contains the SNA15 peptide domains inspired by salivary statherin, a peptide epitope known to specifically bind to and nucleate calcium phosphate. The benefit of using click chemistry is that the hybrid elastin-like-statherin recombinamers cross-link without losing their fibrillar structure. Mineralization of the resulting hybrid elastin-like-statherin recombinamer hydrogels with calcium phosphate is described. Thus, two different hydroxyapatite morphologies (cauliflower- and plate-like) have been formed. Overall, this study shows that crosslinking elastin-like recombinamers leads to interesting matrix materials for the generation of calcium phosphate composites with potential applications as biomaterials.
Qingsheng Qi - One of the best experts on this subject based on the ideXlab platform.
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The production of polyhydroxyalkanoates in Recombinant Escherichia coli
Bioresource Technol, 2007Co-Authors: Ronglin Li, Hong Zhang, Qingsheng QiAbstract:Polyhydroxyalkanoates, the natural polyester that many microorganisms accumulate to store carbon and reducing equivalents, have been considered as a future alternative of traditional plastic due to their special properties. In Escherichia coli, a previous non-polyhydroxyalkanoates producer, pathway engineering has been developed as a very powerful approach to set up microbial production process through the introduction of direct genetic changes by Recombinant DNA Technology. Various metabolic pathways leading to the polyhydroxyalkanoates accumulation with desirable properties at low-cost and high-productivity have been developed. At the same time, high density fermentation Technology of E. coli provides an efficient polyhydroxyalkanoates production strategy. This review focused on metabolic engineering, fermentation and downstream process aiming to polyhydroxyalkanoates production in E. coli.
Mohamed Hamed Misbah - One of the best experts on this subject based on the ideXlab platform.
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Recombinant DNA Technology and click chemistry a powerful combination for generating a hybrid elastin like statherin hydrogel to control calcium phosphate mineralization
Beilstein Journal of Nanotechnology, 2017Co-Authors: Mohamed Hamed Misbah, Mercedes Santos, Luis G Quintanilla, Christina Gunter, Matilde Alonso, Andreas Taubert, Jose Carlos RodriguezcabelloAbstract:Understanding the mechanisms responsible for generating different phases and morphologies of calcium phosphate by elastin-like recombinamers is supreme for bioengineering of advanced multifunctional materials. The generation of such multifunctional hybrid materials depends on the properties of their counterparts and the way in which they are assembled. The success of this assembly depends on the different approaches used, such as Recombinant DNA Technology and click chemistry. In the present work, an elastin-like recombinamer bearing lysine amino acids distributed along the recombinamer chain has been cross-linked via Huisgen [2 + 3] cycloaddition. The recombinamer contains the SNA15 peptide domains inspired by salivary statherin, a peptide epitope known to specifically bind to and nucleate calcium phosphate. The benefit of using click chemistry is that the hybrid elastin-like-statherin recombinamers cross-link without losing their fibrillar structure. Mineralization of the resulting hybrid elastin-like-statherin recombinamer hydrogels with calcium phosphate is described. Thus, two different hydroxyapatite morphologies (cauliflower- and plate-like) have been formed. Overall, this study shows that crosslinking elastin-like recombinamers leads to interesting matrix materials for the generation of calcium phosphate composites with potential applications as biomaterials.
Raymond F Salemme - One of the best experts on this subject based on the ideXlab platform.
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protein engineering for molecular electronics
Current Opinion in Structural Biology, 1992Co-Authors: Stephen G Sligar, Raymond F SalemmeAbstract:Abstract Recombinant DNA Technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices within a general framework outlining strategies for device fabrication. This review is also published in Current Opinion in BioTechnology 1992, 3 :388–394.
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protein engineering for molecular electronics
Current Opinion in Biotechnology, 1992Co-Authors: Stephen G Sligar, Raymond F SalemmeAbstract:Abstract Recombinant DNA Technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabrication. This review is also published in Current Opinion in Structural Biology 1992, 2 :587–592.
Ronglin Li - One of the best experts on this subject based on the ideXlab platform.
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The production of polyhydroxyalkanoates in Recombinant Escherichia coli
Bioresource Technol, 2007Co-Authors: Ronglin Li, Hong Zhang, Qingsheng QiAbstract:Polyhydroxyalkanoates, the natural polyester that many microorganisms accumulate to store carbon and reducing equivalents, have been considered as a future alternative of traditional plastic due to their special properties. In Escherichia coli, a previous non-polyhydroxyalkanoates producer, pathway engineering has been developed as a very powerful approach to set up microbial production process through the introduction of direct genetic changes by Recombinant DNA Technology. Various metabolic pathways leading to the polyhydroxyalkanoates accumulation with desirable properties at low-cost and high-productivity have been developed. At the same time, high density fermentation Technology of E. coli provides an efficient polyhydroxyalkanoates production strategy. This review focused on metabolic engineering, fermentation and downstream process aiming to polyhydroxyalkanoates production in E. coli.