The Experts below are selected from a list of 4107 Experts worldwide ranked by ideXlab platform

Guoxing Nie - One of the best experts on this subject based on the ideXlab platform.

  • effects of Rehmannia glutinosa on growth performance immunological parameters and disease resistance to aeromonas hydrophila in common carp cyprinus carpio l
    Aquaculture, 2015
    Co-Authors: Junli Wang, Xiaolin Meng, Yanting Luo, Xiao Yan, Xianghui Kong, Guoxing Nie
    Abstract:

    Abstract To explore the feasibility of Rehmannia glutinosa (RG) as a fish-feed additive, the effects of RG on the growth performance and the immune function of common carp, Cyprinus carpio L. (C. carpio), were investigated. Dried Rehmannia root powder (DP), prepared Rehmannia root powder (PP), dried Rehmannia root extract (DE) and prepared Rehmannia root extract (PE) were incorporated into the basal diet that was used to feed common carp for 80 d. The 540 juvenile fish with an average weight 7.46 ± 1.06 g were randomly allocated into nine groups, which were each fed one of the following diets: the basal diet as the control (Con), a diet adding 2% DP (LDP), a diet adding 4% DP (HDP), a diet adding 2% PP (LPP), a diet adding 4% PP (HPP), a diet adding 0.5% DE (LDE), a diet adding 1% DE (HDE), a diet adding 0.5% PE (LPE) or a diet adding 1% PE (HPE). The results indicated that the growth performance significantly increased in the groups HDP, LPP, HPP and HDE, compared with the control (P

Huang Luqi - One of the best experts on this subject based on the ideXlab platform.

  • Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein
    MOLECULES, 2016
    Co-Authors: Teng Zhongqiu, Ye Shen, Li Jing, Lin Zhongping, Chen Min, Wang Min, Li Man, Dong Hongran, Huang Luqi
    Abstract:

    Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a top grade herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).National Science Fund for Distinguished Young Scholars [81325023]; China Postdoctoral Science Foundation [2014M551005]; State Administration of Traditional Chinese Medicine, Chinese Medicine Scientific Research Projects [201107011]SCI(E)PubMedARTICLEzhongqiuteng@163.com; shenye70@hotmail.com; justinworking@126.com; linzp@pku.edu.cn; cm315keke@163.com; wangmincacms@sohu.com; liman887@163.com; dhyopp@126.com; huangluqi01@126.com92

Hongran Dong - One of the best experts on this subject based on the ideXlab platform.

  • construction and quality analysis of transgenic Rehmannia glutinosa containing tmv and cmv coat protein
    Molecules, 2016
    Co-Authors: Zhongqiu Teng, Man Li, Min Chen, Ye Shen, Min Wang, Hongran Dong
    Abstract:

    Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a “top grade” herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).

Ye Shen - One of the best experts on this subject based on the ideXlab platform.

  • construction and quality analysis of transgenic Rehmannia glutinosa containing tmv and cmv coat protein
    Molecules, 2016
    Co-Authors: Zhongqiu Teng, Man Li, Min Chen, Ye Shen, Min Wang, Hongran Dong
    Abstract:

    Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a “top grade” herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).

  • Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein
    MOLECULES, 2016
    Co-Authors: Teng Zhongqiu, Ye Shen, Li Jing, Lin Zhongping, Chen Min, Wang Min, Li Man, Dong Hongran, Huang Luqi
    Abstract:

    Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a top grade herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).National Science Fund for Distinguished Young Scholars [81325023]; China Postdoctoral Science Foundation [2014M551005]; State Administration of Traditional Chinese Medicine, Chinese Medicine Scientific Research Projects [201107011]SCI(E)PubMedARTICLEzhongqiuteng@163.com; shenye70@hotmail.com; justinworking@126.com; linzp@pku.edu.cn; cm315keke@163.com; wangmincacms@sohu.com; liman887@163.com; dhyopp@126.com; huangluqi01@126.com92

Junli Wang - One of the best experts on this subject based on the ideXlab platform.

  • effects of Rehmannia glutinosa on growth performance immunological parameters and disease resistance to aeromonas hydrophila in common carp cyprinus carpio l
    Aquaculture, 2015
    Co-Authors: Junli Wang, Xiaolin Meng, Yanting Luo, Xiao Yan, Xianghui Kong, Guoxing Nie
    Abstract:

    Abstract To explore the feasibility of Rehmannia glutinosa (RG) as a fish-feed additive, the effects of RG on the growth performance and the immune function of common carp, Cyprinus carpio L. (C. carpio), were investigated. Dried Rehmannia root powder (DP), prepared Rehmannia root powder (PP), dried Rehmannia root extract (DE) and prepared Rehmannia root extract (PE) were incorporated into the basal diet that was used to feed common carp for 80 d. The 540 juvenile fish with an average weight 7.46 ± 1.06 g were randomly allocated into nine groups, which were each fed one of the following diets: the basal diet as the control (Con), a diet adding 2% DP (LDP), a diet adding 4% DP (HDP), a diet adding 2% PP (LPP), a diet adding 4% PP (HPP), a diet adding 0.5% DE (LDE), a diet adding 1% DE (HDE), a diet adding 0.5% PE (LPE) or a diet adding 1% PE (HPE). The results indicated that the growth performance significantly increased in the groups HDP, LPP, HPP and HDE, compared with the control (P