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Edmondo M Benetti - One of the best experts on this subject based on the ideXlab platform.

Marco Fantin - One of the best experts on this subject based on the ideXlab platform.

Nicholas D Spencer - One of the best experts on this subject based on the ideXlab platform.

Lara J Gamble - One of the best experts on this subject based on the ideXlab platform.

  • time of flight secondary ion mass spectrometry three dimensional imaging of Surface modifications in poly caprolactone scaffold pores
    Journal of Biomedical Materials Research Part A, 2019
    Co-Authors: Michael J Taylor, Daniel J Graham, Lara J Gamble
    Abstract:

    Scaffolds composed of synthetic polymers such as poly(caprolactone) (PCL) are widely used for the support and repair of tissues in biomedicine. Pores are common features in scaffolds as they facilitate cell penetration. Various Surface modifications can be performed to promote key biological responses to these scaffolds. However, verifying the chemistry of these materials post Surface modification is problematic due to the combination of three-dimensional (3D) topography and Surface sensitivity. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is commonly used to correlate Surface chemistry with cell response. In this study, 3D imaging mass spectrometry analysis of Surface modified synthetic polymer scaffolds is demonstrated using PCL porous scaffold, a pore filling polymer sample preparation, and 3D imaging ToF-SIMS. We apply a simple sample preparation procedure, filling the scaffold pores with a poly(vinyl alcohol)/glycerol mixture to remove topographic influence on image quality. This filling method allows the scaffold (PCL) and filler secondary ions to be reconstructed into a 3D chemical image of the pore. Furthermore, we show that Surface modifications in the pores of synthetic polymer scaffolds can be mapped in 3D. Imaging of "dry" and "wet" Surface modifications is demonstrated as well as a comparison of Surface modifications with relatively strong ToF-SIMS peaks (fluorocarbon films [FC]) and to more biologically Relevant Surface modification of a protein (bovine serum albumin [BSA]). We demonstrate that Surface modifications can be imaged in 3D showing that characteristic secondary ions associated with FC and BSA are associated with C3 F8 plasma treatment and BSA, respectively within the pore.

David G. Castner - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of the structure of binary and ternary adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling
    Langmuir, 2003
    Co-Authors: Matthew Scott Wagner, Thomas A. Horbett, David G. Castner
    Abstract:

    In complex adsorbed protein films, the biological reactivity of the adsorbed proteins depends on their relative concentrations, organization, conformation, and orientation. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) samples the outermost composition of an adsorbed protein film, providing biologically Relevant information about the organization and availability of the adsorbed proteins in the film. We have previously shown that ToF-SIMS can quantitatively measure the composition of binary adsorbed protein films. However, for one particular binary system studied (immunoglobulin G−fibrinogen), a difference between the ToF-SIMS and 125I-radiolabeled protein adsorption measurements was reported. It was hypothesized that the composition of the protein film at its outermost (and, therefore, most biologically Relevant) Surface was different than that of the overall protein film due to the protrusion of the adsorbed fibrinogen over the adsorbed immunoglobulin G. This study provides further evidence ...