The Experts below are selected from a list of 42291 Experts worldwide ranked by ideXlab platform

Takeshi Wada - One of the best experts on this subject based on the ideXlab platform.

  • establishment and characterization of a cisplatin Resistant Cell Line kb r derived from oral carcinoma Cell Line kb
    International Journal of Oncology, 2007
    Co-Authors: Kenji Negoro, Yukio Yamano, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Kazuaki Fushimi, Takeshi Wada
    Abstract:

    To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-Resistant Cell Line, KB-R, from CDDP-sensitive oral carcinoma Cell Line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more Resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevated at least five-fold or more in KB-R, compared with KB. The expression status of ATP binding cassette (ABC) transporter genes, which belong to multi-drug resistance genes, was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction and real-time PCR. MRP1 and MRP2 were up-regulated, whereas MDR1 was down-regulated. Pathway and ontology analysis using the Ingenuity Pathway Analysis tool indicated three highly significant genetic networks including 105 of the 1,718 overexpressed genes and one network including 35 'Cell-to-Cell signaling and interaction' related genes. Our results suggested that these Cell Lines, KB and KB-R, may be useful for searching the candidate genes responsible for CDDP-resistance and for further study to understand the mechanism of CDDP-resistance.

  • Establishment and gene analysis of a cisplatin-Resistant Cell Line, Sa-3R, derived from oral squamous Cell carcinoma.
    Oncology reports, 2005
    Co-Authors: Ken Nakatani, Katsuhiro Uzawa, Takeshi Wada, Megumi Nakamura, Naohiko Seki, Hideki Tanzawa, Shigeyuki Fujita
    Abstract:

    Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous Cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-Resistant Cell Line, Sa-3R, from a CDDP-sensitive Cell Line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the Cell Lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R Cell Lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R Cell Line may be, in part, associated with the mechanism.

  • Cross-resistance of platinum derivatives in H-1R, a cisplatin-Resistant Cell Line.
    Oncology reports, 1994
    Co-Authors: Kenji Negoro, Yukio Yamano, Dai Nakashima, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Takeshi Wada
    Abstract:

    We previously established H-1R Cells, a cisplatin (CDDP)-Resistant Cell Line, from H-1 Cells, a CDDP-sensitive oral carcinoma Cell Line. The aim of this study was to identify the molecular mechanism of cross-resistance to antitumor drugs containing a platinum agent in H-1R Cells. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay and clonogenecity assay indicated that H-1R Cells showed strong cross-resistance to carboplatin, nedaplatin and oxaliplatin. The expression status of the copper transporter and organic cation transporters was confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The transporters ATP7A, ATP7B, hCtr1, hOCT1 and hOCT2 were up-regulated, whereas hOCT3 was down-regulated. The Cellular glutathione level was elevated 2-fold in H-1R Cells compared with H-1 Cells. Our results suggested that H-1 and H-1R Cells may be useful in searching for candidate genes responsible for cross-resistance to platinum derivatives and for further studies to understand the mechanism of platinum resistance.

Ken Nakatani - One of the best experts on this subject based on the ideXlab platform.

  • establishment and characterization of a cisplatin Resistant Cell Line kb r derived from oral carcinoma Cell Line kb
    International Journal of Oncology, 2007
    Co-Authors: Kenji Negoro, Yukio Yamano, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Kazuaki Fushimi, Takeshi Wada
    Abstract:

    To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-Resistant Cell Line, KB-R, from CDDP-sensitive oral carcinoma Cell Line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more Resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevated at least five-fold or more in KB-R, compared with KB. The expression status of ATP binding cassette (ABC) transporter genes, which belong to multi-drug resistance genes, was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction and real-time PCR. MRP1 and MRP2 were up-regulated, whereas MDR1 was down-regulated. Pathway and ontology analysis using the Ingenuity Pathway Analysis tool indicated three highly significant genetic networks including 105 of the 1,718 overexpressed genes and one network including 35 'Cell-to-Cell signaling and interaction' related genes. Our results suggested that these Cell Lines, KB and KB-R, may be useful for searching the candidate genes responsible for CDDP-resistance and for further study to understand the mechanism of CDDP-resistance.

  • Establishment and gene analysis of a cisplatin-Resistant Cell Line, Sa-3R, derived from oral squamous Cell carcinoma.
    Oncology reports, 2005
    Co-Authors: Ken Nakatani, Katsuhiro Uzawa, Takeshi Wada, Megumi Nakamura, Naohiko Seki, Hideki Tanzawa, Shigeyuki Fujita
    Abstract:

    Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous Cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-Resistant Cell Line, Sa-3R, from a CDDP-sensitive Cell Line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the Cell Lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R Cell Lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R Cell Line may be, in part, associated with the mechanism.

  • Cross-resistance of platinum derivatives in H-1R, a cisplatin-Resistant Cell Line.
    Oncology reports, 1994
    Co-Authors: Kenji Negoro, Yukio Yamano, Dai Nakashima, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Takeshi Wada
    Abstract:

    We previously established H-1R Cells, a cisplatin (CDDP)-Resistant Cell Line, from H-1 Cells, a CDDP-sensitive oral carcinoma Cell Line. The aim of this study was to identify the molecular mechanism of cross-resistance to antitumor drugs containing a platinum agent in H-1R Cells. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay and clonogenecity assay indicated that H-1R Cells showed strong cross-resistance to carboplatin, nedaplatin and oxaliplatin. The expression status of the copper transporter and organic cation transporters was confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The transporters ATP7A, ATP7B, hCtr1, hOCT1 and hOCT2 were up-regulated, whereas hOCT3 was down-regulated. The Cellular glutathione level was elevated 2-fold in H-1R Cells compared with H-1 Cells. Our results suggested that H-1 and H-1R Cells may be useful in searching for candidate genes responsible for cross-resistance to platinum derivatives and for further studies to understand the mechanism of platinum resistance.

Katsuhiro Uzawa - One of the best experts on this subject based on the ideXlab platform.

  • establishment and characterization of a cisplatin Resistant Cell Line kb r derived from oral carcinoma Cell Line kb
    International Journal of Oncology, 2007
    Co-Authors: Kenji Negoro, Yukio Yamano, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Kazuaki Fushimi, Takeshi Wada
    Abstract:

    To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-Resistant Cell Line, KB-R, from CDDP-sensitive oral carcinoma Cell Line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more Resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevated at least five-fold or more in KB-R, compared with KB. The expression status of ATP binding cassette (ABC) transporter genes, which belong to multi-drug resistance genes, was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction and real-time PCR. MRP1 and MRP2 were up-regulated, whereas MDR1 was down-regulated. Pathway and ontology analysis using the Ingenuity Pathway Analysis tool indicated three highly significant genetic networks including 105 of the 1,718 overexpressed genes and one network including 35 'Cell-to-Cell signaling and interaction' related genes. Our results suggested that these Cell Lines, KB and KB-R, may be useful for searching the candidate genes responsible for CDDP-resistance and for further study to understand the mechanism of CDDP-resistance.

  • Establishment and gene analysis of a cisplatin-Resistant Cell Line, Sa-3R, derived from oral squamous Cell carcinoma.
    Oncology reports, 2005
    Co-Authors: Ken Nakatani, Katsuhiro Uzawa, Takeshi Wada, Megumi Nakamura, Naohiko Seki, Hideki Tanzawa, Shigeyuki Fujita
    Abstract:

    Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous Cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-Resistant Cell Line, Sa-3R, from a CDDP-sensitive Cell Line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the Cell Lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R Cell Lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R Cell Line may be, in part, associated with the mechanism.

  • Cross-resistance of platinum derivatives in H-1R, a cisplatin-Resistant Cell Line.
    Oncology reports, 1994
    Co-Authors: Kenji Negoro, Yukio Yamano, Dai Nakashima, Kengo Saito, Ken Nakatani, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Katsuhiro Uzawa, Takeshi Wada
    Abstract:

    We previously established H-1R Cells, a cisplatin (CDDP)-Resistant Cell Line, from H-1 Cells, a CDDP-sensitive oral carcinoma Cell Line. The aim of this study was to identify the molecular mechanism of cross-resistance to antitumor drugs containing a platinum agent in H-1R Cells. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay and clonogenecity assay indicated that H-1R Cells showed strong cross-resistance to carboplatin, nedaplatin and oxaliplatin. The expression status of the copper transporter and organic cation transporters was confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The transporters ATP7A, ATP7B, hCtr1, hOCT1 and hOCT2 were up-regulated, whereas hOCT3 was down-regulated. The Cellular glutathione level was elevated 2-fold in H-1R Cells compared with H-1 Cells. Our results suggested that H-1 and H-1R Cells may be useful in searching for candidate genes responsible for cross-resistance to platinum derivatives and for further studies to understand the mechanism of platinum resistance.

Gian Luigi Russo - One of the best experts on this subject based on the ideXlab platform.

Shigeyuki Fujita - One of the best experts on this subject based on the ideXlab platform.

  • Establishment and gene analysis of a cisplatin-Resistant Cell Line, Sa-3R, derived from oral squamous Cell carcinoma.
    Oncology reports, 2005
    Co-Authors: Ken Nakatani, Katsuhiro Uzawa, Takeshi Wada, Megumi Nakamura, Naohiko Seki, Hideki Tanzawa, Shigeyuki Fujita
    Abstract:

    Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous Cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-Resistant Cell Line, Sa-3R, from a CDDP-sensitive Cell Line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the Cell Lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R Cell Lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R Cell Line may be, in part, associated with the mechanism.